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1.
Artículo en Inglés | MEDLINE | ID: mdl-38874686

RESUMEN

PURPOSE: To define the spectrum of germline pathogenic variants (PVs) and copy number variant (CNV) in cancer susceptibility genes to the burden of breast and ovarian cancer (BC, OvC) in high-risk Brazilians in Minas Gerais with health insurance, southeast Brazil, undergoing multigene panel testing (MGPT). METHODS: Genotyping eligible individuals with health insurance in the Brazilian healthcare system for Hereditary Breast and Ovarian Cancer Syndrome to undergo molecular testing for 44 or 141-gene panels, a decision that was insurance driven. RESULTS: Overall, 701 individuals clinically defined as high BC/OvC risk, underwent MGPT from 1/2021 to 10/2022, with ~ 50% genotyped with a 44-gene panel and the rest with a 141-gene panel. Overall, 16.4% and 22.6% of genotyped individuals harbored PVs using 44-gene and the 141 gene panel, respectively. The most frequently mutated genes were: BRCA2 (3.7%); BRCA1 (3.6%) and monoallelic MUTYH (3.1%). CONCLUSION: The rate of PVs detected in high-risk individuals in this study was twice the 10% threshold used in Brazilian health guidelines. MGPT doubled the detection rate of PVs in cancer susceptibility genes in high-risk individuals compared with BRCA1/BRCA2 genotyping alone. The spectrum of PVs in Southern Brazil is diverse, with few recurring variants such as TP53 (0.6%), suggesting regional founder effects. The use of MGPT in hereditary cancer in Minas Gerais significantly increased the detection rate of P/LPVs compared to existing guidelines and should be considered as the primary genotyping modality in assessing hereditary cancer risk in Brazil.

2.
J Toxicol Environ Health A ; 87(3): 120-132, 2024 02.
Artículo en Inglés | MEDLINE | ID: mdl-37969104

RESUMEN

There is a growing concern regarding the adverse risks exposure to cylindrospermopsin (CYN) might exert on animals and humans. However, data regarding the toxicity of this cyanotoxin to neotropical fish species are scarce. Using the fish species Poecilia reticulata, the influence of CYN concentrations equal to and above the tolerable for drinking water may produce on liver was determined by assessing biomarkers of antioxidant defense mechanisms and correlated to qualitative and semiquantitative histopathological observations. Adult females were exposed to 0.0 (Control); 0.5, 1 and 1.5 µg/L pure CYN for 24 or 96 hr, in triplicate. Subsequently the livers were extracted for biochemical assays and histopathological evaluation. Catalase (CAT) activity was significantly increased only by 1.5 µg/L CYN-treatment, at both exposure times. Glutathione -S-transferase (GST) activity presented a biphasic response for both exposure times. It was markedly decreased after exposure by 0.5 µg/L CYN treatment but significantly elevated by 1.5 µg/L CYN treatment. All CYN treatments produced histopathological alterations, as evidenced by hepatocyte cords degeneration, steatosis, inflammatory infiltration, melanomacrophage centers, vessel congestion, and areas with necrosis. Further, an IORG >35 was achieved for all treatments, indicative of the presence of severe histological alterations in P. reticulata hepatic parenchyma and stroma. Taken together, data demonstrated evidence that CYN-induced hepatotoxicity in P. reticulata appears to be associated with an imbalance of antioxidant defense mechanisms accompanied by histopathological liver alterations. It is worthy to note that exposure to low environmentally-relevant CYN concentrations might constitute a significant risk to health of aquatic organisms.


Asunto(s)
Toxinas Bacterianas , Poecilia , Animales , Antioxidantes/metabolismo , Toxinas Bacterianas/toxicidad , Hígado/metabolismo , Oxidación-Reducción , Estrés Oxidativo , Uracilo/toxicidad
3.
Int J Mol Sci ; 24(15)2023 Aug 04.
Artículo en Inglés | MEDLINE | ID: mdl-37569805

RESUMEN

Patients with end-stage kidney disease (ESKD) suffer from high levels of protein-bound uremic toxins (PBUTs) that contribute to various comorbidities. Conventional dialysis methods are ineffective in removing these PBUTs. A potential solution could be offered by a bioartificial kidney (BAK) composed of porous membranes covered by proximal tubule epithelial cells (PTECs) that actively secrete PBUTs. However, BAK development is currently being hampered by a lack of knowledge regarding the cytocompatibility of the dialysis fluid (DF) that comes in contact with the PTECs. Here, we conducted a comprehensive functional assessment of the DF on human conditionally immortalized PTECs (ciPTECs) cultured as monolayers in well plates, on Transwell® inserts, or on hollow fiber membranes (HFMs) that form functional units of a BAK. We evaluated cell viability markers, monolayer integrity, and PBUT clearance. Our results show that exposure to DF did not affect ciPTECs' viability, membrane integrity, or function. Seven anionic PBUTs were efficiently cleared from the perfusion fluid containing a PBUTs cocktail or uremic plasma, an effect which was enhanced in the presence of albumin. Overall, our findings support that the DF is cytocompatible and does not compromise ciPTECs function, paving the way for further advancements in BAK development and its potential clinical application.


Asunto(s)
Fallo Renal Crónico , Toxinas Biológicas , Humanos , Diálisis Renal/métodos , Tóxinas Urémicas , Fallo Renal Crónico/terapia , Fallo Renal Crónico/metabolismo , Riñón/metabolismo , Túbulos Renales Proximales/metabolismo , Soluciones para Diálisis/metabolismo , Toxinas Biológicas/metabolismo
4.
Pediatr Nephrol ; 37(12): 2985-2996, 2022 12.
Artículo en Inglés | MEDLINE | ID: mdl-35286457

RESUMEN

Chronic kidney disease (CKD) is a major healthcare burden that takes a toll on the quality of life of many patients. Emerging evidence indicates that a substantial proportion of these patients carry a genetic defect that contributes to their disease. Any effort to reduce the percentage of patients with a diagnosis of nephropathy heading towards kidney replacement therapies should therefore be encouraged. Besides early genetic screenings and registries, in vitro systems that mimic the complexity and pathophysiological aspects of the disease could advance the screening for targeted and personalized therapies. In this regard, the use of patient-derived cell lines, as well as the generation of disease-specific cell lines via gene editing and stem cell technologies, have significantly improved our understanding of the molecular mechanisms underlying inherited kidney diseases. Furthermore, organs-on-chip technology holds great potential as it can emulate tissue and organ functions that are not found in other, more simple, in vitro models. The personalized nature of the chips, together with physiologically relevant read-outs, provide new opportunities for patient-specific assessment, as well as personalized strategies for treatment. In this review, we summarize the major kidney-on-chip (KOC) configurations and present the most recent studies on the in vitro representation of genetic kidney diseases using KOC-driven strategies.


Asunto(s)
Dispositivos Laboratorio en un Chip , Insuficiencia Renal Crónica , Humanos , Calidad de Vida , Riñón , Pruebas Genéticas , Insuficiencia Renal Crónica/genética , Insuficiencia Renal Crónica/terapia
5.
Environ Toxicol ; 37(9): 2244-2258, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-35661388

RESUMEN

Citrate functionalized iron oxide nanoparticles (IONPs) are employed for various purposes-including environmental remediation but the interaction of IONPs with aquatic contaminants is poorly understood. Among those, glyphosate-based herbicides are toxic and affect target organs such as the liver. Evaluations of livers of female Poecilia reticulata by exposures to IONPs at a concentration of 0.3 mg/L were performed with association to: (1) 0.65 mg of glyphosate per litter and (2) 1.3 mg of glyphosate per litter of Roundup Original, and (3) glyphosate P.A at 0.65 mg/L. These associations were carried out progressively, after 7, 14, and 21 days. We detected circulatory disturbances, inflammatory responses, activation of the immune system, regressive changes, and progressive responses with changes in the connective tissue and decreased glycogen reserve from days 14 to 21. Ultrastructural changes in the Disse space and microvilli of hepatocytes indicated decreased contact surface area. In general, the damage was time and concentration dependent, increasing from 7 to 14 days and tending to stabilize from 14 to 21 days. Therefore, herbicide-associated IONPs functioned as xenobiotics inducing intense cellular detoxification processes and activation of hepatic immune responses.


Asunto(s)
Herbicidas , Poecilia , Animales , Femenino , Glicina/análogos & derivados , Herbicidas/toxicidad , Hígado , Nanopartículas Magnéticas de Óxido de Hierro , Poecilia/fisiología , Glifosato
6.
J Trop Pediatr ; 68(3)2022 04 05.
Artículo en Inglés | MEDLINE | ID: mdl-35466380

RESUMEN

OBJECTIVE: To describe the 25-hydroxyvitamin D [25(OH)D] concentrations in children and adolescents and to verify the association with the body mass index z-score (ZBMI), lipid profile and Homeostasis Model Assessment-Insulin Resistance (HOMA-IR). METHOD: Cross-sectional study with 170 children and adolescents aged between 4 and 15 years (106 normal weight and 64 overweight) from a public institution in Santo André-Brazil. Weight, height and waist circumference were verified and ZBMI and waist-to-height ratio (WHtR) were calculated. Biochemical analysis: 25(OH)D levels [deficiency: 25(OH)D < 20 ng/ml]; glycemia and insulin (HOMA-IR), lipid profile and ultra-sensitive C-reactive protein. RESULTS: Mean age was 8.37 ± 3.17 years; 89 (52.4%) were male; 77 (45.3%) Caucasians and 121 (71.2%) pre-pubescent. Overweight was observed in 64 (37.6%), dyslipidemia in 108 (63.5%) and 25(OH)D deficiency in 117 (68.8%) of the individuals. ZBMI (r = -0.209; p = 0.006), WHtR (r = -0.154; p = 0.045), triglycerides (TGs) (r = -0.161; p = 0.037) and TGs/high-density lipoprotein (HDL) ratio (r = -0.168; p = 0.028) were inversely correlated with 25(OH)D concentrations. Overweight children and adolescents were four times more likely to have vitamin D deficiency (odds ratio = 4.28; 95% confidence interval 1.152 to 4.907; p = 0.019), after adjustment for pubertal development (prepubertal), sex (male), HDL-c (<45 mg/dl), non-HDL (>120 mg/dl), TG/HDL ratio (>2.0) and HOMA-IR. CONCLUSIONS: A high prevalence of vitamin D deficiency (68.8%) was observed. There was an independent association between vitamin D deficiency and overweight, not observed for dyslipidemia and insulin resistance. The data point to the need for periodic monitoring of serum concentrations of 25(OH)D and reinforcement of guidelines for combating and preventing overweight in the pediatric age group.


Asunto(s)
Resistencia a la Insulina , Deficiencia de Vitamina D , Adolescente , Índice de Masa Corporal , Calcifediol , Niño , Preescolar , Estudios Transversales , Femenino , Humanos , Masculino , Sobrepeso/epidemiología , Triglicéridos , Vitamina D , Vitaminas
7.
Int J Biometeorol ; 64(2): 243-252, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31250109

RESUMEN

This paper discusses the influence of the user thermal comfort conditions on the time spent in open spaces through a case study performed in Bauru, Sao Paulo, Brazil. The study was carried out in two subspaces of a zoo, under warm and wet conditions. Although the subspaces had different levels of shading, they were of similar interest to visitors. The methodology adopted the collection of objective (air and globe temperature, humidity, and velocity) and subjective data (by application of questionnaires), for a sample of 174 users, and local observations with photographic records each 3 min. Data and images obtained enabled the characterization of the local microclimates and contributed to evaluate (1) user's thermal perception (7-point scale), (2) affective evaluation of the thermal comfort (4-point scale), (3) thermal preference (7-point scale), (4) calculated thermal perception (through PET (physiological equivalent temperature) index), and (5) user's exposure time in each subspace. The great contributions of this work were to identify that in the subspace with bigger shading and less rigorous microclimatic conditions, users had exposure time longer than another more exposed to solar radiation and to validate a method to measure user's exposition time through sequential photos. These results contribute to a discussion that has yet been little explored in the literature of the area, proving the strong influence of the thermal comfort conditions in the users' exposure time in open spaces.


Asunto(s)
Microclima , Sensación Térmica , Brasil , Humedad , Temperatura
8.
Arch Toxicol ; 93(12): 3397-3418, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31664498

RESUMEN

The kidney is frequently involved in adverse effects caused by exposure to foreign compounds, including drugs. An early prediction of those effects is crucial for allowing novel, safe drugs entering the market. Yet, in current pharmacotherapy, drug-induced nephrotoxicity accounts for up to 25% of the reported serious adverse effects, of which one-third is attributed to antimicrobials use. Adverse drug effects can be due to direct toxicity, for instance as a result of kidney-specific determinants, or indirectly by, e.g., vascular effects or crystals deposition. Currently used in vitro assays do not adequately predict in vivo observed effects, predominantly due to an inadequate preservation of the organs' microenvironment in the models applied. The kidney is highly complex, composed of a filter unit and a tubular segment, together containing over 20 different cell types. The tubular epithelium is highly polarized, and the maintenance of this polarity is critical for optimal functioning and response to environmental signals. Cell polarity is dependent on communication between cells, which includes paracrine and autocrine signals, as well as biomechanic and chemotactic processes. These processes all influence kidney cell proliferation, migration, and differentiation. For drug disposition studies, this microenvironment is essential for prediction of toxic responses. This review provides an overview of drug-induced injuries to the kidney, details on relevant and translational biomarkers, and advances in 3D cultures of human renal cells, including organoids and kidney-on-a-chip platforms.


Asunto(s)
Enfermedades Renales/inducido químicamente , Riñón/efectos de los fármacos , Pruebas de Toxicidad/métodos , Animales , Biomarcadores Farmacológicos/análisis , Humanos , Técnicas In Vitro , Riñón/fisiopatología , Pruebas de Toxicidad/instrumentación
9.
Mol Phylogenet Evol ; 100: 322-332, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-27118180

RESUMEN

The Potomida genus (Bivalvia, Unionida) has a Circum-Mediterranean distribution and like other freshwater mussel species, its populations have suffered dramatic declines. Although this genus is currently considered as monotypic, it has a long history of taxonomic revisions and presently many aspects of its systematics and evolutionary history are unclear. We sampled a total of 323 individuals from 39 different sites across the Potomida genus distribution, and sequenced two mitochondrial (16S rDNA and Cytochrome c Oxidase Subunit I) and one nuclear (28S rDNA) genes to clarify its phylogeny and phylogeographic history. Our results show that the genus includes two well-supported clades, one comprising solely the western Mediterranean species Potomida littoralis, and the other including two eastern Mediterranean species, the Greek endemic P. acarnanica and the Anatolian and Middle Eastern P. semirugata. We suggest that Potomida started radiating during the upper Miocene, and that both vicariance and dispersal events shaped the diversification and distribution of the genus along the Mediterranean region. P. littoralis is further divided in two mitochondrial lineages, one restricted to Europe and the other occurring mostly in North Africa. Moreover, some European basins present both lineages in sympatry. The conservation status of the three recognized species should be reevaluated, particularly P. acarnanica, since it is restricted to two Greek river basins presenting a high risk of extinction. Overall, our results clarify some important gaps in knowledge concerning the phylogeny, phylogeography and evolution of the Potomida genus in the Mediterranean region with important taxonomical, ecological and conservational implications.


Asunto(s)
Bivalvos/genética , Animales , Secuencia de Bases , ADN Mitocondrial/genética , Complejo IV de Transporte de Electrones/genética , Evolución Molecular , Agua Dulce , Especiación Genética , Región Mediterránea , Tipificación de Secuencias Multilocus , Filogenia , Filogeografía , ARN Ribosómico 16S/genética
11.
Rev Assoc Med Bras (1992) ; 70(2): e20230816, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38422317

RESUMEN

OBJECTIVE: The aim of this study was to analyze the prescription of packed red blood cells performed by emergency physicians for adults with sickle cell anemia. METHODS: Transfusions performed in adults with sickle cell anemia treated at an emergency service in São Bernardo do Campo, São Paulo Brazil, between January 2018 and January 2022 were evaluated. For data comparison, the chi-square2 test was used. The significance level adopted was 5%. RESULTS: A total of 114 transfusions were performed. The mean age was 41.8±16.4 years, and pretransfusion hemoglobin was 6.1±1.23 g/dL. Regarding the indication, the adequacy of transfusions performed in symptomatic individuals was significantly higher compared to asymptomatic individuals (100% vs. 3.9%, p<0.001). Symptomatic individuals received excessive volumes of packed red blood cells less frequently than asymptomatic individuals (17.5% vs. 56.9%, p<0.001). The filtered subtype, indicated for sickle cell anemia, was prescribed in only a quarter of the patients. However, non-indicated subtypes were frequently prescribed. CONCLUSION: This study found low adequacy in the indication and calculation of the transfusion volume of packed red blood cells in asymptomatic individuals. Few patients received filtered red blood cells, resulting in increased risks of transfusion reactions. On the contrary, non-indicated subtypes were prescribed in a quarter of transfusions, which resulted in higher costs and delay in receiving packed red blood cells.


Asunto(s)
Anemia de Células Falciformes , Adulto , Humanos , Persona de Mediana Edad , Brasil , Anemia de Células Falciformes/terapia , Transfusión Sanguínea , Hospitales , Eritrocitos
12.
Microbiol Resour Announc ; : e0117823, 2024 Mar 27.
Artículo en Inglés | MEDLINE | ID: mdl-38534151

RESUMEN

We report the draft genome sequence of Agarivorans aestuarii strain ZMCS4, isolated from Cladostephus spongiosus. The assembled genome consists of 4.5 Mbp, comprising 25 contigs and 4,128 coding sequences. This genome will provide insights into further studies on relevant CAZymes involved in the hydrolysis of algal cell walls.

13.
Eval Rev ; : 193841X231197741, 2023 Aug 23.
Artículo en Inglés | MEDLINE | ID: mdl-37610037

RESUMEN

To improve one of the lowest rates of literacy and numeracy in the world, the government of Brazil has targeted public education reform, given the strong link between an educated population and economic growth. This study examines the academic performance of the Brazilian public primary school system. It addresses the empirical shortcomings of prior research to examine the dynamics of the relationship between academic performance scores and several demographic and institutional variables, such as socioeconomic characteristics, variations in school infrastructure and school complexity, and teachers' human capital. We employed quantile regression to explore the determinants of academic performance across 35,490 schools in rural and urban environments in Brazil. The dependent variable in our analysis captures the academic performance score, as measured by Brazil's education authorities, of each school in our dataset. The model includes several education-related indices used in prior research and, as explanatory factors, measures of teachers' human capital and the students' socioeconomic level, which synthesizes information on parents' education and household income. The results suggest that several institutional variables, including access to school libraries, computer facilities, projectors, and televisions, are positively and significantly related to the academic performance of primary students in Brazil's system of public education. Furthermore, students' socioeconomic level is positively associated with their academic performance.

14.
Einstein (Sao Paulo) ; 21: eAO0251, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37341220

RESUMEN

OBJECTIVE: To compare serum amyloid A concentrations between overweight and eutrophic children and adolescents and to relate it to lipid profiles, glucose tolerance, and carotid intima-media thickness. METHODS: One hundred children and adolescents (mean age: 10.8±3.16 years) were included and divided into two groups: overweight and non-overweight. The following were evaluated: Z-score body mass index, carotid intima-media thickness, lipid metabolism biomarkers (lipid profile and apolipoproteins A1 and B), inflammatory biomarkers (ultra-sensitive C-reactive protein and serum amyloid A), and glucose homeostasis model assessment of insulin resistance. RESULTS: The groups were homogeneous in age, sex, and pubertal stage. Higher levels of triglycerides, apolipoprotein B, homeostasis model assessment of insulin resistance, ultrasensitive C-reactive protein, serum amyloid A, and carotid intima-media thickness were observed in the overweight group. In the multivariate analysis, age (OR=1.73; 95%CI: 1.16-2.60, p=0.007), Z-score body mass index (OR=3.76; 95%CI: 1.64-8.59, p=0.002), apolipoprotein-B (OR=1.1; 95%CI: 1.01-1.2, p=0.030), and carotid intima-media thickness (OR=5.00; 95%CI: 1.38-18.04, p=0.014) were independently associated with serum amyloid A levels above the fourth quartile of the studied sample (>9.4mg/dL). CONCLUSION: Overweight children and adolescents had higher serum amyloid A concentrations than eutrophic children. There was an independent association between higher concentrations of serum amyloid A and Z-score, body mass index, apolipoprotein B, and carotid intima-media thickness, indicating the importance of this inflammatory biomarker in identifying the early risk of atherosclerosis.


Asunto(s)
Grosor Intima-Media Carotídeo , Resistencia a la Insulina , Humanos , Adolescente , Niño , Proteína C-Reactiva , Proteína Amiloide A Sérica , Glucosa , Sobrepeso
15.
J Cardiovasc Pharmacol Ther ; 28: 10742484231169644, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37194899

RESUMEN

INTRODUCTION: Ticagrelor might reduce infarct size by exerting a more potent antiplatelet effect or by promoting a potential conditioning stimulus in ST-elevation myocardial infarction (STEMI) patients. Pre-infarction angina (PIA) is an effective preconditioning stimulus that reduces ischemia-reperfusion injury. Because little is known on the interaction of PIA in STEMI-patients loaded with ticagrelor, we sought to determine if patients loaded with ticagrelor had improved clinical outcomes as compared to clopidogrel and to study if it is modulated by the presence of PIA. METHODS: From 1272 STEMI patients submitted to primary percutaneous coronary intervention and treated with clopidogrel or ticagrelor from January 2008 to December 2018, 826 were analyzed after propensity score matching. Infarct size was estimated using peak creatine kinase (CK) and troponin T (TnT), and clinical impact was evaluated through cumulative major cardiac and cerebrovascular events (MACCE) at 1-year follow-up. Matched patients and their interaction with PIA were analyzed. RESULTS: Patients loaded with ticagrelor had lower peak CK [1405.50 U/L (730.25-2491.00), P < .001] and TnT [3.58 ng/mL (1.73-6.59), P < .001)], regardless of PIA. The presence of PIA was associated with lower CK (P = .030), but not TnT (P = .097). There was no interaction between ticagrelor loading and PIA (P = .788 for TnT and P = .555 for CK). There was no difference in MACCE incidence between clopidogrel or ticagrelor loading (P = .129). Cumulative survival was also similar between clopidogrel or ticagrelor, regardless of PIA (P = .103). CONCLUSION: Ticagrelor reduced infarct sizes independently and without a synergic effect with PIA. Despite reducing infarct size, clinical outcomes were similar across both groups.


Asunto(s)
Intervención Coronaria Percutánea , Infarto del Miocardio con Elevación del ST , Humanos , Ticagrelor/efectos adversos , Clopidogrel/efectos adversos , Infarto del Miocardio con Elevación del ST/diagnóstico , Infarto del Miocardio con Elevación del ST/tratamiento farmacológico , Inhibidores de Agregación Plaquetaria/efectos adversos , Angina de Pecho/tratamiento farmacológico , Intervención Coronaria Percutánea/efectos adversos , Resultado del Tratamiento
16.
Rev Assoc Med Bras (1992) ; 69(2): 285-290, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36722654

RESUMEN

OBJECTIVE: The aim of this study was to describe homocysteine concentrations in overweight and obese children and adolescents and relate them to blood pressure levels, renal function, and insulin resistance. METHODS: This is a cross-sectional and observational study with 64 overweight children and adolescents (mean age: 11.6±3.5 years) in outpatient follow-up. The following parameters were evaluated: body mass index z-score, waist-to-height circumference ratio, pubertal stage, blood pressure, serum homocysteine, glycemia, insulin, lipid profile, renal function, high-sensitivity C-reactive protein, microalbuminuria, and creatinuria. Statistical analysis: analysis of variance and logistic regression (dependent variable: homocysteine) (p<0.05). RESULTS: The mean body mass index z-score was 2.9±1.1. The mean homocysteine concentrations were 8.6±2.2 µmol/L (10th and 90th percentiles: 6.6 and 11.2 µmol/L, respectively), with no difference when compared with children with severe obesity and obesity/overweight (p=0.431). High values of waist-to-height ratio (93.8%), systolic blood pressure (18.8%), diastolic blood pressure (12.5%), glycemia (4.7%), low-density lipoprotein cholesterol (31.1%), triglycerides (35.9%), non-high-density lipoprotein cholesterol (34.4%), and microalbuminuria (21.9%) were obtained. The mean glomerular filtration rate was 122.9±24.6 mL/min/1.73 m². Homocysteine concentrations were not associated with any of the studied variables (R²=0.095). CONCLUSION: Homocysteine concentrations in overweight children and adolescents (mean 8.6±2.2 µmol/L) were not associated with body mass index z-score, blood pressure, renal function, and insulin resistance.


Asunto(s)
Homocisteína , Resistencia a la Insulina , Sobrepeso , Obesidad Infantil , Adolescente , Niño , Humanos , Presión Sanguínea/fisiología , Índice de Masa Corporal , Estudios Transversales , Sobrepeso/complicaciones , Obesidad Infantil/complicaciones , Factores de Riesgo , Homocisteína/sangre
17.
Stem Cell Res Ther ; 14(1): 353, 2023 12 10.
Artículo en Inglés | MEDLINE | ID: mdl-38072933

RESUMEN

BACKGROUND: Ischemia/reperfusion injury is the leading cause of acute kidney injury (AKI). The current standard of care focuses on supporting kidney function, stating the need for more efficient and targeted therapies to enhance repair. Mesenchymal stromal cells (MSCs) and their secretome, either as conditioned medium (CM) or extracellular vesicles (EVs), have emerged as promising options for regenerative therapy; however, their full potential in treating AKI remains unknown. METHODS: In this study, we employed an in vitro model of chemically induced ischemia using antimycin A combined with 2-deoxy-D-glucose to induce ischemic injury in proximal tubule epithelial cells. Afterwards we evaluated the effects of MSC secretome, CM or EVs obtained from adipose tissue, bone marrow, and umbilical cord, on ameliorating the detrimental effects of ischemia. To assess the damage and treatment outcomes, we analyzed cell morphology, mitochondrial health parameters (mitochondrial activity, ATP production, mass and membrane potential), and overall cell metabolism by metabolomics. RESULTS: Our findings show that ischemic injury caused cytoskeletal changes confirmed by disruption of the F-actin network, energetic imbalance as revealed by a 50% decrease in the oxygen consumption rate, increased oxidative stress, mitochondrial dysfunction, and reduced cell metabolism. Upon treatment with MSC secretome, the morphological derangements were partly restored and ATP production increased by 40-50%, with umbilical cord-derived EVs being most effective. Furthermore, MSC treatment led to phenotype restoration as indicated by an increase in cell bioenergetics, including increased levels of glycolysis intermediates, as well as an accumulation of antioxidant metabolites. CONCLUSION: Our in vitro model effectively replicated the in vivo-like morphological and molecular changes observed during ischemic injury. Additionally, treatment with MSC secretome ameliorated proximal tubule damage, highlighting its potential as a viable therapeutic option for targeting AKI.


Asunto(s)
Lesión Renal Aguda , Vesículas Extracelulares , Células Madre Mesenquimatosas , Humanos , Secretoma , Isquemia/terapia , Isquemia/metabolismo , Vesículas Extracelulares/metabolismo , Lesión Renal Aguda/terapia , Lesión Renal Aguda/metabolismo , Metabolismo Energético , Oxidación-Reducción , Células Madre Mesenquimatosas/metabolismo , Adenosina Trifosfato/metabolismo
18.
Chemosphere ; 328: 138590, 2023 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-37028726

RESUMEN

Iron oxide nanoparticles (IONPs) are advanced materials for water remediation technologies. It is therefore relevant to evaluate the cellular and tissue behavior of fishes in response to IONPs and their associations with agrochemicals such as glyphosate (GLY) and glyphosate-based herbicides (GBHs). Iron accumulation, tissue integrity and lipid distribution in the hepatocytes of Poecilia reticulata (guppy) were investigated in a control group and in groups exposed to soluble iron ions, namely IFe (0.3 mgFe/L), IONPs (0.3 mgFe/L), and IONPs, associated with GLY (0.65 mg/L), GBHs 0.65 mgGLY/L (IONPs + GBH1), and 1.30 mgGLY/L (IONPs + GBH2), for 7, 14, and 21 days, followed by an equal period of postexposure in clean reconstituted water. The results showed that the accumulation of iron was greater in the subjects in the IONP treatment group when compared to that in the Ife group. In addition, the subjects in the mixtures with GBHs had a greater accumulation of iron than those in the IONP + GLY treatment group. Tissue integrity assessments demonstrated an intense accumulation of lipids, formation of necrotic zones and leukocyte infiltrates in all the treated groups, with a greater quantity of lipids in the animals treated with IONP + GLY and IFe. During postexposure, the results indicated an elimination of iron in all treated groups, reaching the same level as the control group, throughout the 21 days postexposure. Thus, the damage caused to animal livers by IONP mixtures is reversible, providing promising results for the development of safe environmental remediation practices using nanoparticles.


Asunto(s)
Herbicidas , Hepatopatías , Nanopartículas , Poecilia , Animales , Herbicidas/toxicidad , Hierro , Nanopartículas Magnéticas de Óxido de Hierro , Lípidos , Compuestos Férricos , Glifosato
19.
Environ Toxicol Pharmacol ; 100: 104144, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37149012

RESUMEN

Iron oxide nanoparticles (IONP) are promising alternatives to environmental remediation, so this study investigates IONP single and associated to contaminants, in this case, glyphosate (GLY) and Roundup® (GBH) in Poecilia reticulata (guppy). The guppies have internal development, therefore this study analyzed female gonads to establish the developmental stages of P. reticulata and evaluate effects of exposure (7, 14 and 21 days) and post-exposure (same period) to the treatments with Iron ions 0.3 mg Fe/L (IFe); IONP 0.3 mg Fe/L; IONP 0.3 mgFe/L + GBH 0,65 mgGLY/L (IONP+GBH1); IONP 0.3 mgFe/L + GBH 1.30 mgGLY/L (IONP+GBH2); and IONP 0.3 mgFe/L + GLY 0.65 mg/L (IONP+GLY). The development was organized in immature, development, and gestation phases. The damage in all treatments after 21 days of exposure was evident in reaction patterns regressive inflammatory, and circulatory including total histopathologic index of liver, nevertheless there was a damage recovery trend during post-exposure period.


Asunto(s)
Ovario , Poecilia , Animales , Femenino , Hígado , Nanopartículas Magnéticas de Óxido de Hierro , Glifosato
20.
Rev Assoc Med Bras (1992) ; 68(12): 1698-1704, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36477102

RESUMEN

OBJECTIVE: The aim of this study was to describe the prevalence of anemia and iron deficiency anemia (IDA) in infants and verify the association of iron deficiency with nutritional status. METHODS: This cross-sectional and observational study included 104 infants aged between 7 and 9 months, assisted from August to September 2021 by the Family Health Strategy program in Sousa municipality (Paraíba, Brazil). Clinical and anthropometric data were collected, and a 24-h food recall questionnaire was applied using the DietPro software (version 5.0) in order to verify food consumption and assess iron intake. Variables associated with iron deficiency (p<0.05) were analyzed using multiple logistic regression. RESULTS: Anemia and IDA were observed in 40.4% and 19.2% of infants, respectively. Only one infant was taking prophylactic supplementation (ferrous sulfate). Infants with IDA presented reduced hemoglobin (p<0.001) and ferritin (p<0.001) and increased Z-scores of body mass index-for-age (Z-BMI) (p=0.027), weight-for-height (p=0.007), and weight-for-age (p=0.032). All Z-scores were inversely correlated with ferritin (Z-BMI [rho: -0.37; p<0.001], weight-for-height [rho: -0.37; p<0.001], and weight-for-age [rho: -0.29; p=0.002]). Ferritin was also directly correlated with daily iron intake (rho: 0.22; p=0.018). Finally, multiple logistic regression showed a significant and direct association of iron deficiency with weight-for-height Z-score (odds ratio: 2.86; 95% confidence interval: 1.38-5.64; p=0.004). CONCLUSION: About 60% of infants presented anemia or IDA. Iron deficiency was associated with the weight-for-height Z-score, showing the vulnerability of infants during the introduction of complementary feeding.


Asunto(s)
Anemia Ferropénica , Anemia , Deficiencias de Hierro , Lactante , Humanos , Anemia Ferropénica/epidemiología , Hierro/metabolismo , Estado Nutricional , Brasil/epidemiología , Estudios Transversales , Ferritinas , Hemoglobinas/análisis , Prevalencia
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