RESUMEN
OBJECTIVE: To compare the expression of stem cell-related genes in the endometrium (END), superficial endometriosis (SE), and deep infiltrating endometriosis (DIE). STUDY DESIGN: We performed a prospective pilot study of six women suffering from SE and DIE who gave consent for laparoscopy surgery, endometrial biopsies, and participation in this study. Quantitative RT-PCR analysis of 84 stem cell-related genes was performed in 18 biopsy samples. RESULTS: A total of 40 of 84 genes were expressed in SE and DIE, but were different from END as follows. Seven genes were over-expressed in SE and 33 genes were under-expressed in DIE compared with END. Two genes were only over-expressed in SE and three genes were only over-expressed in DIE. Six under-expressed genes were exclusively located in SE and one was only located in DIE. The remaining 31 genes were not different among the groups. There was no significant difference in gene expression between SE and DIE samples. CONCLUSION: Tissue of DIE and SE appears to have similar stem cell-related genes. Nevertheless, there are differences in gene expression between SE and DIE.
Asunto(s)
Endometriosis/genética , Endometrio/metabolismo , ARN Mensajero/metabolismo , Células Madre/metabolismo , Adulto , Biopsia , Endometriosis/metabolismo , Endometriosis/patología , Femenino , Expresión Génica , Humanos , Laparoscopía , Proyectos Piloto , Estudios Prospectivos , Adulto JovenRESUMEN
The objective of this study was to assess the feasibility and safety of laparoscopic rectosigmoid anterior wall discoid resection for endometriosis using the circular stapler. A retrospective analysis was conducted of nine consecutive patients undergoing laparoscopic radical excision of pelvic endometriosis, including bowel anterior wall discoid excision, at the Fertility and Pelvic Surgery Clinic and private hospitals in São Paulo, Brazil. The selected intervention was a radical laparoscopic endometriosis resection, including rectosigmoid anterior wall excision with the circular stapler. For certain types of bowel endometriosis, the anterior wall discoid stapler excision proved a suitable option that diminishes the chances of serious complications such as bowel fistula or anastomosis dehiscence.