Meta-analysis of salt marsh vegetation impacts and recovery: a synthesis following the Deepwater Horizon oil spill.

Marine oil spills continue to be a global issue, heightened by spill events such as the 2010 Deepwater Horizon spill in the Gulf of Mexico, the largest marine oil spill in US waters and among the largest worldwide, affecting over 1,000 km of sensitive wetland shorelines, primarily salt marshes supporting numerous ecosystem functions. To synthesize the effects of the oil spill on foundational vegetation species in the salt marsh ecosystem, Spartina alterniflora and Juncus roemerianus, we performed a meta-analysis using data from 10 studies and 255 sampling sites over seven years post-spill. We examined the hypotheses that the oil spill reduced plant cover, stem density, vegetation height, aboveground biomass, and belowground biomass, and tracked the degree of effects temporally to estimate recovery time frames. All plant metrics indicated impacts from oiling, with 20-100% maximum reductions depending on oiling level and marsh zone. Peak reductions of ~70-90% in total plant cover, total aboveground biomass, and belowground biomass were observed for heavily oiled sites at the marsh edge. Both Spartina and Juncus were impacted, with Juncus affected to a greater degree. Most plant metrics had recovery time frames of three years or longer, including multiple metrics with incomplete recovery over the duration of our data, at least seven years post-spill. Belowground biomass was particularly concerning, because it declined over time in contrast with recovery trends in most aboveground metrics, serving as a strong indicator of ongoing impact, limited recovery, and impaired resilience. We conclude that the Deepwater Horizon spill had multiyear impacts on salt marsh vegetation, with full recovery likely to exceed 10 years, particularly in heavily oiled marshes, where erosion may preclude full recovery. Vegetation impacts and delayed recovery is likely to have exerted substantial influences on ecosystem processes and associated species, especially along heavily oiled shorelines. Our synthesis affords a greater understanding of ecosystem impacts and recovery following the Deepwater Horizon oil spill, and informs environmental impact analysis, contingency planning, emergency response, damage assessment, and restoration efforts related to oil spills.

Coastal eutrophication as a driver of salt marsh loss.

Salt marshes are highly productive coastal wetlands that provide important ecosystem services such as storm protection for coastal cities, nutrient removal and carbon sequestration. Despite protective measures, however, worldwide losses of these ecosystems have accelerated in recent decades. Here we present data from a nine-year whole-ecosystem nutrient-enrichment experiment. Our study demonstrates that nutrient enrichment, a global problem for coastal ecosystems, can be a driver of salt marsh loss. We show that nutrient levels commonly associated with coastal eutrophication increased above-ground leaf biomass, decreased the dense, below-ground biomass of bank-stabilizing roots, and increased microbial decomposition of organic matter. Alterations in these key ecosystem properties reduced geomorphic stability, resulting in creek-bank collapse with significant areas of creek-bank marsh converted to unvegetated mud. This pattern of marsh loss parallels observations for anthropogenically nutrient-enriched marshes worldwide, with creek-edge and bay-edge marsh evolving into mudflats and wider creeks. Our work suggests that current nutrient loading rates to many coastal ecosystems have overwhelmed the capacity of marshes to remove nitrogen without deleterious effects. Projected increases in nitrogen flux to the coast, related to increased fertilizer use required to feed an expanding human population, may rapidly result in a coastal landscape with less marsh, which would reduce the capacity of coastal regions to provide important ecological and economic services.

A test of biological trait analysis with nematodes and an anthropogenic stressor.

Aquatic ecosystems are fundamentally altered by nutrient enrichment, and effective monitoring tools are needed to detect biological responses especially in the early stages of eutrophication. We tested the utility of biological trait analysis (BTA) to quantify the temporal responses of nematodes inhabiting salt marsh creeks that were experimentally enriched with nutrients for 6 years. Feeding, body shape, and tail shape traits were characterized on >6000 nematodes from annual samples from enriched and non-enriched sites. Here, we ask if trait combinations are more effective than single traits in detecting the magnitude and rate of change. We also sought to identify combinations of traits that best distinguish natural from nutrient-induced variation. BTA revealed that feeding, body shape, and all traits combined equally detected the response to nutrient enrichment. Compared to single traits however, BTAs were more sensitive to temporal trends and better distinguished natural variation from the response to nutrient enrichment. Tail shape traits (that might respond to altered sediment texture or geochemistry) were not affected by enrichment, and feeding traits yielded the greatest difference between enriched and reference communities indicating that changes in food resources drove responses. Feeding traits provided the highest quality information content in our study, and the use of feeding traits alone may adequately identify anthropogenic effects in many studies. However, we caution that body shape, tail shape, and feeding traits were strongly interrelated at our study site, and a diversity of trait groups may increase the information content of BTAs in more diverse habitats.

Toxicity and bioaccumulation of TNT in marine fish in sediment exposures.

The bioaccumulation potential and toxicity of 2,4,6-trinitrotoluene (TNT) spiked to sediment was evaluated in juvenile sheepshead minnows (JSHM, Cyprinodon variegatus) and adult freckled blennies (FB, Hypsoblennius ionthas). The JSHM were exposed for 4 days in the presence or absence of a mesh separating fish from sediment. FB were exposed to sediment for 7 days. During the 24-day storage period (4 °C), extensive transformation of spiked TNT occurred and concentrations are expressed as the sum of TNT, aminodinitrotoluenes and diaminonitrotoluenes (SumTNT), on a dry weight basis. SumTNT in the overlying water, not exchanged during exposure, increased gradually. Survival was high (≥ 90%) for JSHM exposed to 7 mg kg(-1) and FB exposed to up to 260 mg kg(-1). All SHM died after 24 h exposure to 340 mg kg(-1). Isolation from sediment did not significantly affect water concentrations or decrease bioaccumulation. Uptake from contact to sediment was likely negligible and bioaccumulation was from the overlying water. The feeding rate of FB exposed to 1700 µmol kg(-1) sediment suspended in water for 24-h was significantly reduced by 50%.

Recovery of the salt marsh periwinkle (Littoraria irrorata) 9 years after the Deepwater Horizon oil spill: Size matters.

Prior studies indicated salt marsh periwinkles (Littoraria irrorata) were strongly impacted in heavily oiled marshes for at least 5 years following the Deepwater Horizon oil spill. Here, we detail longer-term effects and recovery over nine years. Our analysis found that neither density nor population size structure recovered at heavily oiled sites where snails were smaller and variability in size structure and density was increased. Total aboveground live plant biomass and stem density remained lower over time in heavily oiled marshes, and we speculate that the resulting more open canopy stimulated benthic microalgal production contributing to high spring periwinkle densities or that the lower stem density reduced the ability of subadults and small adults to escape predation. Our data indicate that periwinkle population recovery may take one to two decades after the oil spill at moderately oiled and heavily oiled sites, respectively.

Effects of diesel and interactions with copper and other metals in an estuarine sediment microbial community.

Estuarine sediment microcosms were treated with combinations of diesel, copper (at two levels), and a mixture of heavy metals (mercury, cadmium, lead, and chromium; at two levels) mimicking the contaminant loadings found in harbor sediments. The effects on the microbial community were monitored by polar lipid fatty acid analysis. Diesel addition increased microbial biomass, caused shifts in some fatty acid structural groups, and decreased starvation biomarkers. Incorporation of diesel hydrocarbons into lipids was expressed as an increase in the proportion of odd-carbon-number fatty acids. No treatment with the metals mixture (mercury, cadmium, lead, and chromium) alone significantly changed any parameter derived from the polar lipid fatty acids, but the increase in microbial biomass from diesel addition was higher with the metals mixture, possibly because of indirect effects caused by reductions in grazing resulting from metal-induced toxicity to bacteriovorous nematodes. Copper also modified the effects of diesel addition, preventing biomass increase but not diesel degradation, suggesting that some of the energy gained from diesel oxidation was expended combating copper toxicity. In the present study, observations indicate that metals in general, and copper in particular, can modify the response of sedimentary microorganisms to petroleum-hydrocarbon contaminants.

Effects of phenanthrene- and metal-contaminated sediment on the feeding activity of the Harpacticoid Copepod, Schizopera knabeni.

The effects of sediments contaminated with sublethal concentrations of phenanthrene (PAH) and metals (Cd, Hg, Pb) were evaluated in relation to their influence on the feeding activity of a harpacticoid copepod, Schizopera knabeni. A metal mixture (at the ratio of 5Pb:3Cd:2Hg) and Cd alone reduced grazing rates of S. knabeni feeding on (14)C-labeled microalgae. Cadmium alone and Cd combined with phenanthrene significantly decreased grazing rates of S. knabeni at Cd concentrations above 49 mg kg(-1) dry sediment. No grazing was observed in 98, 106, or 157 mg kg(-1) dry sediment Cd alone or in sediment contaminated with phenanthrene (98 mg kg(-1) dry sediment) combined with Cd at these concentrations. Phenanthrene alone also caused a significant decrease (55%) in S. knabeni grazing rates. Feeding ceased above 344 mg kg(-1) dry sediment of the metal mixture alone and combined with phenanthrene. Results were consistent with an independent effect on feeding when Cd and phenanthrene were combined. When other metals were added (Pb and Hg) to the mixture, results were consistent with an additive influence on feeding rate. Because the underlying mechanisms of toxicity for metals and PAH are probably different, our observations suggest that reductions in grazing probably did not directly contribute to the lethal effects of phenanthrene or metals. The absence of interactive effects on feeding suggests that metal-PAH interactive effects on lethality have a different underlying mechanism and that reductions in grazing probably did not directly contribute to the lethality effects of phenanthrene or metals in S. knabeni.

Legacy effects of Hurricane Katrina influenced marsh shoreline erosion following the Deepwater Horizon oil spill.

Disturbance interactions occur when one perturbation influences the severity and perhaps the baseline state of succeeding disturbances. Natural and anthropogenic disturbances are frequent in dynamic coastal ecosystems and can often be linked. We evaluated potential for disturbance interactions associated with the 2010 Deepwater Horizon (DWH) oil spill, which was preceded by disturbance from Hurricane Katrina in 2005, by quantifying marsh shoreline retreat across both events. Our goal was to determine the degree to which Hurricane Katrina altered baseline rates of erosion prior to the DWH spill. We quantified erosion rate and fetch from aerial images of northern Barataria Bay, Louisiana marsh shorelines classified as reference, moderately-oiled, and heavily-oiled over three pre-spill time periods (1998-2004, prior to Hurricane Katrina; 2004-2005, during Katrina; 2005-2010, post-Katrina but pre-oil spill) and a post-spill period from 2010 to 2013. Prior to Hurricane Katrina, marsh shoreline erosion rates were low (from 0.38 to 1.10 m yr-1). In contrast during Hurricane Katrina (2004-2005), erosion increased by 661% and 756%, respectively, for shorelines that would subsequently become moderately and heavily-oiled; reference shoreline erosion increased by 59%. These high erosion rates were associated with increased fetch and higher wave action due to loss of protective geomorphic features such as small islands and spits and persisted during the post-Katrina/pre-spill period of 2005-2010 (0.62, 1.38, and 2.07 m yr-1 for reference, moderately, and heavily-oiled shorelines, respectively). Erosion rates increased modestly after the DWH event (reference = 1.13 m yr-1, moderate oiling = 1.45 m yr-1; heavy oiling = 2.77 m yr-1), but not significantly, compared to the post-Katrina period. Consequently, we could not detect a post-spill increase in marsh shoreline erosion. Rather, we concluded that Hurricane Katrina reset the erosion baseline, thereby connecting the two disturbances, and was the major driver of marsh shoreline erosion at our research sites during the study period.

Mixtures of metals and polynuclear aromatic hydrocarbons elicit complex, nonadditive toxicological interactions in meiobenthic copepods.

The acute toxicity of metal-polynuclear aromatic hydrocarbon (PAH) mixtures (i.e., Cd, Hg, Pb, fluoranthene, and phenanthrene) associated with sediments was assessed in two benthic copepods. Schizopera knabeni was exposed to sediment amended with single contaminants and mixtures. Adult S. knabeni were highly tolerant of single-contaminant exposures to phenanthrene, Cd, Hg, and Pb as well as a mixture of Cd, Hg, and Pb. Binary experiments revealed that although phenanthrene was synergistic with Cd and Hg, the phenanthrene-Cd synergism was much stronger (2.8 times more lethal than predicted). When a mixture of Cd, Hg, and Pb was combined with phenanthrene, a synergistic response was observed, eliciting 1.5 times greater lethality than predicted. A Cd-phenanthrene synergism in S. knabeni was also observed in aqueous exposures, suggesting that the interaction was related to a pharmacological insult rather than a sediment-related exposure effect. An antagonism between Cd, Hg, and Pb was indicated, and this antagonism may have moderated the Cd-phenanthrene synergism in mixtures containing Cd, Hg, Pb, and phenanthrene. Experiments with Amphiascoides atopus revealed that phenanthrene and fluoranthene were each synergistic with Cd in aqueous exposures. Our studies suggest that interactive toxicity among metal-PAH mixtures may be common among benthic copepods and that strong synergistic effects observed in binary mixtures may be moderated in more diverse contaminant mixtures. However, the strength of the observed synergisms raises concerns that established sediment quality criteria may not be protective for organisms jointly exposed to PAH and metals, especially Cd-PAH mixtures.

Shoreline oiling effects and recovery of salt marsh macroinvertebrates from the Deepwater Horizon Oil Spill.

Salt marshes in northern Barataria Bay, Louisiana, USA were oiled, sometimes heavily, in the aftermath of the Deepwater Horizon oil spill. Previous studies indicate that fiddler crabs (in the genus Uca) and the salt marsh periwinkle (Littoraria irrorata) were negatively impacted in the short term by the spill. Here, we detail longer-term effects and recovery from moderate and heavy oiling over a 3-year span, beginning 30 months after the spill. Although neither fiddler crab burrow density nor diameter differed between oiled and reference sites when combined across all sampling events, these traits differed among some individual sampling periods consistent with a pattern of lingering oiling impacts. Periwinkle density, however, increased in all oiling categories and shell-length groups during our sampling period, and periwinkle densities were consistently highest at moderately oiled sites where Spartina alterniflora aboveground biomass was highest. Periwinkle shell length linearly increased from a mean of 16.5 to 19.2 mm over the study period at reference sites. In contrast, shell lengths at moderately oiled and heavily oiled sites increased through month 48 after the spill, but then decreased. This decrease was associated with a decline in the relative abundance of large adults (shell length 21-26 mm) at oiled sites which was likely caused by chronic hydrocarbon toxicity or oil-induced effects on habitat quality or food resources. Overall, the recovery of S. alterniflora facilitated the recovery of fiddler crabs and periwinkles. However, our long-term record not only indicates that variation in periwinkle mean shell length and length-frequency distributions are sensitive indicators of the health and recovery of the marsh, but agrees with synoptic studies of vegetation and infaunal communities that full recovery of heavily oiled sites will take longer than 66 months.

Effects of nutrient enrichment, depuration substrate, and body size on the trophic transfer of cadmium associated with microalgae to the benthic amphipod Leptocheirus plumulosus.

Bioavailability and nutrient effects on the trophic transfer of Cd associated with microalgae to the marine benthic amphipod Leptocheirus plumulosus were investigated. Cadmium assimilation efficiency (AE) of suspension-feeding L. plumulosus significantly varied among three algal species tested (Nitzschia punctata, Thalassiosira weissflogii, and Isochrysis galbana). Depuration substrate greatly influenced Cd AE for L. plumulosus (AE was much higher for nonburrowed amphipods), probably because sediment burrowing allowed L. plumulosus to feed as a surface deposit feeder. The L. plumulosus body size, ranging from 0.5 to 2.0 mm, did not affect Cd AE. Nitrate enrichment from 0 to 180 microM in algal culture significantly increased Cd AE from 9.4 to 18.8% for T. weissflogii, from 10.0 to 27.3% for N. punctata, and from 10.0 to 16.2% for I. galbana; nitrate enrichment from 0 to 60 microM did not influence Cd AE in any algal species tested. Physiological turnover rate constants of Cd in L. plumulosus ranged from 0.016 to 0.025/h for the three species and were independent of nitrate addition. Nitrate enrichment strongly enhanced Cd distribution in algal cytoplasm. Phosphate enrichment (0-7.5 miroM) did not significantly affect Cd AEs in L. plumulosus. Overall, a significant linear relationship was observed between the Cd AE of L. plumulosus and the fraction of Cd available in algal cytoplasm. Our work suggests that eutrophication by nitrate enrichment has the potential to enhance the trophic transfer of Cd from microalgae to suspension-feeding benthic invertebrates.

Bioavailability of polycyclic aromatic hydrocarbons in field-contaminated Anacostia River (Washington, DC) sediment.

Sediment-water partitioning behavior and bioavailability of five polycyclic aromatic hydrocarbons (PAHs; phenanthrene, pyrene, chrysene, benzo[k]fluoranthene, and benzo[a]pyrene) were measured in field-contaminated sediment collected from moderately polluted regions of the Anacostia River (Washington, DC, U.S.A.). Much of the sediment PAH burden was resistant to desorption: Effective partition coefficients were 2- to 10-fold greater than expected from literature values, and more than 80% of PAHs remained sorbed after treatment of the sediment with a nonionic polymeric adsorbent (Amberlite XAD-2) for 20 h. Bioaccumulation, elimination, and assimilation of each PAH in the deposit-feeding tubificid oligochaete Ilyodrilus templetoni were measured and compared with the equivalent measurements from laboratory-inoculated sediment. Ilyodrilus templetoni effectively accessed the desorption-resistant fraction of these organic contaminants, as exhibited by high single-gut passage assimilation efficiencies (ASEs) of the five PAHs (60% < ASE < 90%). However, steady-state accumulations of PAHs by I. templetoni were very low and consistent with low pore-water concentrations. The present results suggest that steady-state accumulation of PAHs is controlled by pore-water concentrations and is not necessarily related to route of uptake or assimilation efficiencies.

Response of salt marshes to oiling from the Deepwater Horizon spill: Implications for plant growth, soil surface-erosion, and shoreline stability.

We investigated the initial impacts and post spill recovery of salt marshes over a 3.5-year period along northern Barataria Bay, LA, USA exposed to varying degrees of Deepwater Horizon oiling to determine the effects on shoreline-stabilizing vegetation and soil processes. In moderately oiled marshes, surface soil total petroleum hydrocarbon concentrations were ~70mgg(-1) nine months after the spill. Though initial impacts of moderate oiling were evident, Spartina alterniflora and Juncus roemerianus aboveground biomass and total live belowground biomass were equivalent to reference marshes within 24-30months post spill. In contrast, heavily oiled marsh plants did not fully recover from oiling with surface soil total petroleum hydrocarbon concentrations that exceeded 500mgg(-1) nine months after oiling. Initially, heavy oiling resulted in near complete plant mortality, and subsequent recovery of live aboveground biomass was only 50% of reference marshes 42months after the spill. Heavy oiling also changed the vegetation structure of shoreline marshes from a mixed Spartina-Juncus community to predominantly Spartina; live Spartina aboveground biomass recovered within 2-3years, however, Juncus showed no recovery. In addition, live belowground biomass (0-12cm) in heavily oiled marshes was reduced by 76% three and a half years after the spill. Detrimental effects of heavy oiling on marsh plants also corresponded with significantly lower soil shear strength, lower sedimentation rates, and higher vertical soil-surface erosion rates, thus potentially affecting shoreline salt marsh stability.

Exposure-related effects on Cd bioaccumulation explain toxicity of Cd-phenanthrene mixtures in Hyalella azteca.

Little is known regarding mixture effects of metals and polynuclear aromatic hydrocarbon (PAHs) under environmentally relevant exposure regimes. Standard U.S. Environmental Protection Agency (U.S. EPA) procedures were applied and extended to test effects of phenanthrene (Phen) on sediment-Cd uptake, aqueous-Cd uptake, and Cd-elimination kinetics in the amphipod Hyalella azteca. In sediment exposures, Phen increased the projected equilibrium-tissue concentration of Cd from 47.2 (36.2-58.3) to 221.1 microg/g ([117.8-324.3], 95% confidence intervals [CI] in parentheses). Although Cd bioaccumulation increased markedly in sediment exposures, dissolved Cd concentrations and physical-chemical parameters indicative of Cd bioavailability were unaffected by Phen. Further, in water-only exposures, Phen had no effect on Cd bioaccumulation or Cd-elimination kinetics. These results indicate that increased Cd bioaccumulation in Cd-Phen mixtures occurred via a sediment-mediated process and was likely a function of increased uptake associated with feeding (i.e., Phen-induced alterations in ingestion and/or digestive processes). Observed increases in H. azteca lethality when exposed to Cd-Phen mixtures in sediment, but not in water-only exposures, likely resulted from increased Cd bioaccumulation rate rather than a true toxicological synergism. Thus, apparent synergisms and antagonisms may result from exposure-mediated effects in sediment that are unrelated to toxicological interactions. Implications of these findings regarding sediment-quality assessment and suggestions for future studies are discussed.

Facilitative and Inhibitory Interactions Among Estuarine Meiobenthic Harpacticoid Copepods.

In the meiofauna communities of Louisiana estuaries (USA), the harpacticoid copepods Scottolana canadensis and Pseudostenhelia wellsi are predominant species. Scottolana canadensis is a semisessile burrow-dweller capable of subsurface suspension and deposit feeding. Pseudostenhelia wellsi is also semisessile but builds extensive networks of mucus tubes within the top 1 cm of muddy sediments, and appears to graze on its inner tubewalls. Tube building by P. wellsi generates meiofauna-sized structure and adds cohesiveness to surface sediments, as well as providing potential food and grazing substrates for other meiofuna. Monospecific patches of P. wellsi and S. canadensis (250 individuals/5 cm2 ) were artificially generated in laboratory microcosms to determine if the unique lifestyle and sedimentary effects of either species facilitate or inhibit colonization by two other errant, burrowing harpacticoids common in the community, Nitrocra lacustris and Cletocamptus deitersi. These two species share similar foraging and burrowing behaviors and similar effects on sediment structure, which sharply contrast with those of P. wellsi and S. canadensis. Pseudostenhelia wellsi tube patches facilitated colonization by both S. canadensis and N. lacustris, but strongly inhibited colonization by C. deitersi. Scottolana canadensis patches were unattractive to N. lacustris. As P. wellsi showed the strongest effects on colonization by other harpacticoids, its mechanisms of facilitation/inhibition were also studied. In laboratory microcosms, cultured S. canadensis and N. lacustris were offered patches of azoic sediments, mucin-enriched sediments without structure, azoic sediments with agar tube mimics (structure), and sediments with natural P. wellsi tubes (mucus and structure). Both mucus enrichment and inert tube structure acted as strong facilitants to N. lacustris copepodites and adults overall. However, neither effect alone facilitated patch colonization by N. lacustris adult females and S. canadensis copepodites and adults. Their colonization was facilitated specifically by natural P. wellsi tubes. These experiments demonstrate that species interactions in harpacticoid communities can quickly influence spatial patterns, and those patterns may be mediated by species-specific effects on the sedimentary environment (e.g., mucus tube, burrows, increased flushing, erodability, etc.). However, spatial patterns cannot be predicted easily by contrasting the compatibility of one species' biogenic effects with those of another.

Indirect effects of contaminants in aquatic ecosystems.

Contaminants such as petroleum hydrocarbons, heavy metals and pesticides can cause direct toxic effects when released into aquatic environments. Sensitive species may be impaired by sublethal effects or decimated by lethality, and this ecological alteration may initiate a trophic cascade or a release from competition that secondarily leads to responses in tolerant species. Contaminants may exert direct effects on keystone facilitator and foundation species, and contaminant-induced changes in nutrient and oxygen dynamics may alter ecosystem function. Thus, populations and communities in nature may be directly and/or indirectly affected by exposure to pollutants. While the direct effects of toxicants usually reduce organism abundance, indirect effects may lead to increased or decreased abundance. Here we review 150 papers that reference indirect toxicant effects in aquatic environments. Studies of accidental contaminant release, chronic contamination and experimental manipulations have identified indirect contaminant effects in pelagic and benthic communities caused by many types of pollutants. Contaminant-induced changes in behavior, competition and predation/grazing rate can alter species abundances or community composition, and enhance, mask or spuriously indicate direct contaminant effects. Trophic cascades were found in 60% of the manipulative studies and, most commonly, primary producers increased in abundance when grazers were selectively eliminated by contaminants. Competitive release may also be common, but is difficult to distinguish from trophic cascades because few experiments are designed to isolate the mechanism(s) causing indirect effects. Indirect contaminant effects may have profound implications in environments with strong trophic cascades such as the freshwater pelagic. In spite of their undesirable environmental influence, contaminants can be useful manipulative tools for the study of trophic and competitive interactions in natural communities.

Bioavailability and assimilation of sediment-associated benzo[a]pyrene by Ilyodrilus templetoni (Oligochaeta).

Benzo[a]pyrene (BaP)-amended sediment was desorbed by a sequential batch method using an isopropanol solution wash. The observed isotherm showed no evidence of desorption resistance, as indicated by increased partitioning to the solid phase at low concentrations. This was consistent with the prediction of minimal desorption resistance for highly hydrophobic compounds using a biphasic model. Bioavailability of BaP in desorbed sediments was assessed by toxicokinetic measures of uptake, bioaccumulation, and elimination in the deposit-feeding, freshwater tubificid oligochaete Ilyodrilus templetoni. Worms were exposed to sediments with BaP concentrations of approximately 26 and 11 microg/g dry weight sediment after desorption for one and three batches, respectively. The I. templetoni tissue concentration attained an apparent steady state after approximately one month and resulted in a biota-sediment accumulation factor of approximately 1.3 for both sediments. This is consistent with the paradigm that pore-water concentration predicts the uptake of organic contaminants into lipids despite the literature data showing that the major uptake route for BaP is likely from the ingestion of sediment particles. Ilyodrilus templetoni exhibited a high assimilation efficiency (80%) during a single-gut passage, a low elimination rate (k(c) = 0.0032 h(-1)), and negligible biotransformation of sediment-associated BaP.

Differential tolerance among cryptic species: a potential cause of pollutant-related reductions in genetic diversity.

Differential mortality of cryptic species (i.e., morphologically similar but genetically distinct sibling species) may contribute to observed reductions in genetic diversity at contaminated sites if the members of a complex of cryptic species exhibit differential responses to the contaminants that are present. We conducted toxicity bioassays with both polynuclear aromatic hydrocarbon and metal contamination on Cletocamptus fourchensis and C. stimpsoni from two intensively sampled locations. Previous molecular and detailed morphological analyses segregated these as cryptic species from the cosmopolitan C. deitersi. We found that these species occur together at two field sites and that they exhibit unique toxic responses to heavy metals, suggesting differential tolerances at contaminated sites. These findings suggest that reported losses of genetic diversity at contaminated sites may represent a reduction in species diversity rather than a loss of the presumed less-tolerant genotypes within a species. They also suggest that members of a cryptic species complex should not be used in laboratory toxicity tests unless populations are genetically characterized. Future studies using genetic diversity as a marker of contaminant effects should consider the possibility of undetected cryptic species.

Bioavailability of desorption-resistant phenanthrene to the oligochaete Ilyodrilus templetoni.

We investigated bioavailability, as measured by the biota-sediment accumulation factor (BSAF), of reversibly sorbed and desorption-resistant phenanthrene to the deposit-feeding freshwater tubificid oligochaete Ilyodrilus templetoni. Desorption-resistant, phenanthrene-contaminated sediments were prepared by a sequential batch desorption method by washing with an isopropanol solution. The BSAFs averaged 1.20 +/- 0.32 and 0.59 +/- 0.13 for reversibly sorbed phenanthrene and desorption-resistant phenanthrene, respectively, indicating a significantly reduced bioavailability of desorption-resistant phenanthrene. A generalized model assuming a linear relationship between pore-water concentration and normalized bioaccumulation described 91% of the variance for both measured and selected literature data of the BSAF. The reduced bioavailibility of desorption-resistant phenanthrene was thus well described by physical and chemical measures of partitioning between pore water and sediment.

The toxicological interaction between ocean acidity and metals in coastal meiobenthic copepods.

Increased atmospheric CO(2) concentrations are causing greater dissolution of CO(2) into seawater, and are ultimately responsible for today's ongoing ocean acidification. We manipulated seawater acidity by addition of HCl and by increasing CO(2) concentration and observed that two coastal harpacticoid copepods, Amphiascoides atopus and Schizopera knabeni were both more sensitive to increased acidity when generated by CO(2). The present study indicates that copepods living in environments more prone to hypercapnia, such as mudflats where S. knabeni lives, may be less sensitive to future acidification. Ocean acidification is also expected to alter the toxicity of waterborne metals by influencing their speciation in seawater. CO(2) enrichment did not affect the free-ion concentration of Cd but did increase the free-ion concentration of Cu. Antagonistic toxicities were observed between CO(2) with Cd, Cu and Cu free-ion in A. atopus. This interaction could be due to a competition for H(+) and metals for binding sites.