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1.
J Arthroplasty ; 2024 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-39089393

RESUMEN

BACKGROUND: This study investigated the perioperative outcomes of patients undergoing conversion total hip arthroplasty (THA) after failed peri-hip bone flap grafting (PBFG) and compared them with those patients undergoing primary THA for osteonecrosis of the femoral head (ONFH). METHODS: From January 2010 to December 2021, 163 Chinese patients (163 hips) were treated by conversion THA after failed PBFG (containing 94 patients who had pedicled vascularized iliac bone flap grafting and 69 patients who had pedicled vascularized greater trochanter bone flap grafting), and 178 Chinese patients were treated by primary THA. The preoperative baseline data and perioperative indicators in both groups were compared. RESULTS: In the conversion group, patients had significantly greater blood loss, a longer length of stay, and greater changes in serum hemoglobin than those in the primary THA group (P < 0.05). The operative room time, transfusion volume, calculated blood loss, changes in serum hematocrit, and increased superficial infection (P > 0.05) in the conversion group were greater compared with the primary cohort; however, the difference was not statistically significant. The mean postoperative Harris Hip Scoring System (HHS) of the PBFG group at the one-month follow-up was 81, and the control group had an 82 score. Importantly, subgroup analysis of the PBFG group indicated that there was no significant difference between patients who had prior pedicled vascularized iliac bone flap grafting and pedicled vascularized greater trochanter bone flap grafting (P > 0.05), except for the operative room time (P = 0.032). CONCLUSION: Hip-sparing surgery of ONFH did not make THA more difficult or lead to more peri-operative complications, but increased blood loss and extended hospital stay from a prior PBFG are still notable problems in clinical practice. Thus, it is necessary for surgeons to focus attention on the improvement of the preoperative condition and prepare for any specific intraoperative challenges.

2.
BMC Musculoskelet Disord ; 23(1): 999, 2022 Nov 19.
Artículo en Inglés | MEDLINE | ID: mdl-36401231

RESUMEN

BACKGROUND: Recently, tranexamic acid (TXA) and epsilon aminocaproic acid (EACA) have been applied in total hip arthroplasty (THA). However, doubts in clinicians' minds about which medicine is more efficient and economical in THA need to be clarified. Therefore, this study compared the efficacy and cost of the intraoperative administration of TXA and EACA per surgery in decreasing perioperative blood transfusion rates in THA. METHODS:  This study enrolled patients who underwent THA between January 2019 to December 2020. A total of 295 patients were retrospectively divided to receive topical combined with intravenous TXA (n = 94), EACA (n = 97) or control (n = 104). The primary endpoints included transfusions, estimated perioperative blood loss, cost per patient and the drop in the haemoglobin and haematocrit levels. RESULTS: Patients who received EACA had greater total blood loss, blood transfusion rates, changes in HGB levels and mean cost of blood transfusion per patient (P < 0.05) compared with patients who received TXA. In addition, both TXA and EACA groups had significantly fewer perioperative blood loss, blood transfusion, operation time and changes in haemoglobin and haematocrit levels than the control group (P < 0.05). Cost savings in the TXA and EACA groups were 736.00 RMB and 408.00 RMB per patient, respectively. CONCLUSIONS: The application of perioperative antifibrinolytics notably reduces the need for perioperative blood transfusions. What's more, this study demonstrated that TXA is superior to EACA for decreasing blood loss and transfusion rates while at a lower cost per surgery. These results indicate that TXA may be the optimum antifibrinolytics for THA in Chinese area rather than EACA.


Asunto(s)
Antifibrinolíticos , Artroplastia de Reemplazo de Cadera , Ácido Tranexámico , Humanos , Artroplastia de Reemplazo de Cadera/efectos adversos , Estudios Retrospectivos , Pérdida de Sangre Quirúrgica/prevención & control , Aminocaproatos , Ácido Aminocaproico , Hemoglobinas
3.
Plant Cell Rep ; 39(10): 1369-1380, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32712731

RESUMEN

KEY MESSAGE: Arabidopsis, tobacco, tomato and rice with merA/merB expressed reduced mercury concentration of leaves, fruits or grains. These mercury-breathing plants produce agricultural products with acceptable levels of mercury from contaminated soil. Mercury contamination in plant food products can cause serious health risks to consumers. Transgenic approaches to enhance mercury phytoremediation have been accomplished with expression of bacterial merA and merB genes to convert toxic organic mercury to less toxic elemental mercury. However, little is known whether these genes can be used to produce safe foods from plants grown on mercury-contaminated land. We have used Arabidopsis and tobacco as model plants for leafy vegetables, and tomato and rice as representative fruit and grain crops to investigate whether merA and merB expression allows for production of safe foods from mercury-contaminated soils. Our results show that grown on heavily contaminated land with mercury, merA and merB expressing transgenic plants can produce vegetables, fruits and grains safe for human and animal consumption, while the wild-type plants cannot. The merA and merB transgenic plants can also efficiently remove mercury from soil. With increasing mercury contamination problems for the agricultural land worldwide, the use of the merA and merB genes can help produce safe food from mercury-polluted land and also remediate contaminated soils.


Asunto(s)
Mercurio/análisis , Proteínas de Plantas/genética , Plantas/metabolismo , Semillas/metabolismo , Contaminantes del Suelo/toxicidad , Verduras/metabolismo , Biodegradación Ambiental/efectos de los fármacos , Culinaria , Frutas/efectos de los fármacos , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Cloruro de Mercurio/toxicidad , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Proteínas de Plantas/metabolismo , Plantas/efectos de los fármacos , Plantas/genética , Plantas Modificadas Genéticamente , Semillas/efectos de los fármacos , Semillas/crecimiento & desarrollo , Verduras/efectos de los fármacos , Verduras/crecimiento & desarrollo
4.
Ann Plast Surg ; 85(6): 677-684, 2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-32501844

RESUMEN

INTRODUCTION: Nontraumatic osteonecrosis of the femoral head (NONFH) is a common and difficult disease in orthopedics. Magnetic resonance imaging (MRI) assessment of NONFH and bone marrow edema was combined with digital subtraction angiography (DSA) to evaluate the circulatory status of NONFH in different Association Research Circulation Osseous stages. Based on the circulatory obstruction status (venous stasis, arterial ischemia, and arterial occlusion), appropriate perioperative management was adopted to achieve hip joint preservation and effectively delay the time for total hip arthroplasty in young patients. METHODS: From January 2013 to March 2019, 41 orthopedic patients were evaluated for medical imaging. Sixty-one ONFH cases were enrolled. The inclusion criteria include: (1) Clear diagnosis of osteonecrosis of the femoral head. (2) No history of infection in the affected hip, no history of hip surgery, and no congenital hip diseases. The patients enrolled in this study were 8 women and 33 men between the ages of 19 and 64 years (mean, 39.25 ± 8.90 years). Preoperative X-ray, computed tomography, MRI, DSA, and histological data were taken. RESULTS: The combination of DSA and MRI can efficiently show blood supply changes in the femoral head of NONFH patients at different Association Research Circulation Osseous stages; and also can possibly reveal the causes and development of NONFH. Different stages of circulatory obstruction of the femoral head can be clearly distinguished and used to determine the required perioperative management, thus yielding successful surgical outcomes. CONCLUSIONS: The existing classification systems do not fully reflect the progression of circulatory obstruction in ONFH. Each stage of NONFH development has its own characteristics circulatory obstruction. Early-stage NONFH displays characteristic venous stasis of the femoral head, whereas advanced stage NONFH is characterized by insufficient arterial blood supply to the femoral head. Corresponding NONFH treatment strategies should be considered based on their specific circulatory status. This work also provides guidance and recommendations for adopting corresponding femoral head preserving strategies for young patients in different NONFH circulatory status.


Asunto(s)
Necrosis de la Cabeza Femoral , Cabeza Femoral , Adulto , Angiografía de Substracción Digital , Femenino , Fémur , Cabeza Femoral/diagnóstico por imagen , Necrosis de la Cabeza Femoral/diagnóstico por imagen , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Adulto Joven
5.
Planta ; 250(1): 145-162, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-30949762

RESUMEN

MAIN CONCLUSION: The possible molecular mechanisms regulating strawberry fruit ripening were revealed by plant hormone quantification, exogenous hormone application, and RNA-sequencing. Fruit ripening involves a complex interplay among plant hormones. Strawberry is a model for studies on non-climacteric fruit ripening. However, the knowledge on how plant hormones are involved in strawberry ripening is still limited. To understand hormonal actions in the ripening process, we performed genome-wide transcriptome and hormonal analysis for the five major hormones (abscisic acid and catabolites, auxins, cytokinins, gibberellins, and ethylene) in achenes and receptacles (flesh) at different ripening stages of the woodland strawberry Fragaria vesca. Our results demonstrate that the pre-turning stage (a stage with white flesh and red achenes defined in this study) is the transition stage from immature to ripe fruits. The combinatorial analyses of hormone content, transcriptome data, and exogenous hormone treatment indicate that auxin is synthesized predominantly in achenes, while abscisic acid (ABA), bioactive free base cytokinins, gibberellins, and ethylene are mainly produced in receptacles. Furthermore, gibberellin may delay ripening, while ethylene and cytokinin are likely involved at later stages of the ripening process. Our results also provide additional evidence that ABA promotes ripening, while auxin delays it. Although our hormone analysis demonstrates that the total auxin in receptacles remains relatively low and unchanged during ripening, our experimental evidence further indicates that ABA likely enhances expression of the endoplasmic reticulum-localized auxin efflux carrier PIN-LIKES, which may subsequently reduce the auxin level in nucleus. This study provides a global picture for hormonal regulation of non-climacteric strawberry fruit ripening and also evidence for a possible mechanism of ABA and auxin interaction in the ripening process.


Asunto(s)
Fragaria/genética , Regulación de la Expresión Génica de las Plantas , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/metabolismo , Transcriptoma , Ácido Abscísico/análisis , Ácido Abscísico/metabolismo , Citocininas/análisis , Citocininas/metabolismo , Etilenos/análisis , Etilenos/metabolismo , Fragaria/fisiología , Frutas/genética , Frutas/fisiología , Giberelinas/análisis , Giberelinas/metabolismo , Ácidos Indolacéticos/análisis , Ácidos Indolacéticos/metabolismo , Reguladores del Crecimiento de las Plantas/análisis , Proteínas de Plantas/genética
6.
Int Orthop ; 43(5): 1083-1087, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-29876626

RESUMEN

OBJECTIVE: This study aims to investigate the early diagnosis and treatment of steroid-induced osteonecrosis of the femoral head. PATIENTS AND METHODS: From January 2010 to January 2014, a total of 350 patients, who required the use of large amounts of hormones, were enrolled into the study. These patients were followed up every three months after starting the hormone therapy. A total of 62 cases were screened, among which nine cases were asymptomatic. Furthermore, 38 patients were diagnosed as stage I and were given low-molecular weight heparin (LMWH) and vasodilator drugs. Moreover, 22 cases were diagnosed as stage IIa/b and underwent core decompression. In addition, two cases were diagnosed as stage IIc and underwent pedicled bone transplantation. During the follow-up period, ARCO staging was used for radiological evaluation, the HHS score was applied to evaluate for clinical efficacy, and SPSS 22.0 statistical software was used for the data analysis. RESULTS: A total of 60 patients were followed up for 24 months. Among these patients, 38 patients were diagnosed with ARCO stage I and underwent systematic therapy. No progress was found in 29 cases (76.3%). Furthermore, three cases progressed to stage IIb (7.8%), four cases progressed to stage IIc (10.5%), two cases progressed to stage III and IV, respectively (2.6%), and 16 cases (80%) did not progress after core decompression. In the 16 cases at stage IIa and four cases at stage IIb, and four cases (20%) progressed in stage III. The HHS score of stage I was 80.42 ± 3.25 before follow-up, while the HHS score was 86.46 ± 8.54 after follow-up, and the difference was statistically significant (P < 0.05). Furthermore, the HHS score of patients with stage IIa/b was 70.38 ± 4.62 before follow-up, while the HHS score was 80.28 ± 6.72 after follow-up, and the difference was statistically significant (P < 0.01). CONCLUSION: MRI remains as the most effective method for the non-invasive diagnosis of osteonecrosis, at present. Enhanced MRI may be able to detect early osteonecrosis, but further research is needed. Drug treatment and core decompression can achieve satisfactory results at the early stage.


Asunto(s)
Necrosis de la Cabeza Femoral/diagnóstico por imagen , Necrosis de la Cabeza Femoral/terapia , Cabeza Femoral/cirugía , Adolescente , Adulto , Angiografía de Substracción Digital , Anticoagulantes/administración & dosificación , Trasplante Óseo , Descompresión Quirúrgica , Diagnóstico Precoz , Femenino , Cabeza Femoral/efectos de los fármacos , Necrosis de la Cabeza Femoral/inducido químicamente , Necrosis de la Cabeza Femoral/clasificación , Estudios de Seguimiento , Heparina de Bajo-Peso-Molecular/uso terapéutico , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Resultado del Tratamiento , Vasodilatadores , Adulto Joven
7.
J Biol Chem ; 291(12): 6200-17, 2016 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-26792857

RESUMEN

SCCRO (squamous cell carcinoma-related oncogene; also known as DCUN1D1) is a highly conserved gene that functions as an E3 in neddylation. Although inactivation of SCCRO in yeast results in lethality, SCCRO(-/-) mice are viable. The exclusive presence of highly conserved paralogues in higher organisms led us to assess whether compensation by SCCRO paralogues rescues lethality in SCCRO(-/-) mice. Using murine and Drosophila models, we assessed the in vivo activities of SCCRO and its paralogues in cullin neddylation. We found that SCCRO family members have overlapping and antagonistic activity that regulates neddylation and cell proliferation activities in vivo. In flies, both dSCCRO and dSCCRO3 promote neddylation and cell proliferation, whereas dSCCRO4 negatively regulates these processes. Analysis of somatic clones showed that the effects that these paralogues have on proliferation serve to promote cell competition, leading to apoptosis in clones with a net decrease in neddylation activity. We found that dSCCRO and, to a lesser extent, dSCCRO3 rescue the neddylation and proliferation defects promoted by expression of SCCRO4. dSCCRO and dSCCRO3 functioned cooperatively, with their coexpression resulting in an increase in both the neddylated cullin fraction and proliferation activity. In contrast, human SCCRO and SCCRO4 promote, and human SCCRO3 inhibits, neddylation and proliferation when expressed in flies. Our findings provide the first insights into the mechanisms through which SCCRO family members cooperatively regulate neddylation and cell proliferation.


Asunto(s)
Proteínas Cullin/metabolismo , Procesamiento Proteico-Postraduccional , Proteínas Proto-Oncogénicas/fisiología , Animales , Proliferación Celular , Proteínas de Drosophila/fisiología , Drosophila melanogaster , Femenino , Células HeLa , Humanos , Péptidos y Proteínas de Señalización Intracelular , Masculino , Ratones Noqueados , Especificidad de Órganos
8.
Zhonghua Yi Xue Za Zhi ; 94(21): 1627-30, 2014 Jun 03.
Artículo en Zh | MEDLINE | ID: mdl-25152284

RESUMEN

OBJECTIVE: To analyze the progression of asymptomatic osteonecrosis of the femoral head by evaluating its lesion size and location. METHODS: From January 2008 to June 2009, 24 cases or hips were available for outcome analysis. There were 16 males and 8 females with a mean age of 36.4 (22-46) years and a mean body mass index of 25.4 (17.5-35). The stages were Ia (n = 2), b (n = 4), IIa (n = 6), IIb (n = 8) and IIc (n = 4) according to ARCO staging system. The etiologies included corticosteroids (n = 16), alcohol abuse (n = 4) and idiopathic disease (n = 4). Magnetic resonance imaging (MRI) was used to measure lesion sizes with necrotic index method. And lesion locations were classified as medial, central and lateral. RESULTS: The mean follow-up period was 40.3 (32-60) months. Among 7 painful hips, there were corticosteroids (n = 5) and alcohol abuse (n = 2). And 5 hips (20.8%) collapsed. The mean interval between diagnosis and symptom was 40 (36-48) months. The lesion locations were lateral-central (n = 4) lateral-central-medial (n = 3). Significant difference existed between the average necrotic index of symptomatic and asymptomatic hips (45.4% vs 32.7%). CONCLUSION: There is a high risk of progression into symptomatic disease when the necrotic lesion occurs on the lateral and lateral-central or lateral-central-medial zone of the femoral head. Asymptomatic small and medially located lesions may be managed conservatively with close medical imaging observations.


Asunto(s)
Necrosis de la Cabeza Femoral/patología , Osteonecrosis/patología , Adulto , Progresión de la Enfermedad , Femenino , Cadera , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Adulto Joven
9.
Microsurgery ; 33(8): 656-9, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24115327

RESUMEN

The aim of this report is to present our experience on the use of the digital subtraction angiography (DSA) in selection of the vascularized greater trochanter bone grafting for the treatment of the osteonecrosis of femoral head (ONFH) in early stages. Between January 2005 and June 2007, DSA was used to evaluate the blood perfusion of the early stages ONFH in 32 patients (45 hips). There were 18 males and 14 females with an average age of 30 years old. Twenty-one hips were in ARCO stage I, and 24 in ARCO stage II. The arterial blood supply insufficiency was found in 22 hips by DSA, and the venous stasis in 23 hips. The hips with artery blood supply insufficiency received the vascularized greater trochanter bone grafting, and the hips with the venous stasis received the core decompression. All of patients were followed-up with an average of 4.8 years (ranging 2.4-6.6 years). The preoperative Harris Hip score (HHS) in the patients with arterial blood supply insufficiency was 48.18 ± 7.81 and the postoperative HHS was 93.27 ± 3.03. The preoperative HHS in the patients with venous stasis was 44.04 ± 6.40, and the postoperative HHS 92.65 ± .93. The postoperative DSA showed an improved perfusion of the femoral head in 44 hips. Our experience showed that DSA would help to select the appropriate procedure for treatment of ONFH in the early stage.


Asunto(s)
Angiografía de Substracción Digital , Trasplante Óseo/métodos , Necrosis de la Cabeza Femoral/cirugía , Fémur/trasplante , Adolescente , Adulto , Femenino , Fémur/irrigación sanguínea , Fémur/diagnóstico por imagen , Estudios de Seguimiento , Humanos , Masculino , Cuidados Posoperatorios , Cuidados Preoperatorios , Resultado del Tratamiento , Adulto Joven
10.
Gene ; 794: 145753, 2021 Aug 20.
Artículo en Inglés | MEDLINE | ID: mdl-34090961

RESUMEN

The use of male sterile lines is one of the ideal means in hybrid seed production. Despite the widespread application of Ogura cytoplasmic male sterile (CMS) lines, the molecular mechanisms remain largely unknown. In this study, histological analyses of floral buds from a CMS line 40MA and its corresponding maintainer line 40MB were conducted, which indicate that microspore abortion was initiated shortly after the tetrad stage. RNA sequencing was performed to analyze the transcriptomes of floral buds from the tetrad stage and the early microspore stages of these two lines. More than 39 million clean reads were generated for each library, and the portions mapped to the reference genome were all above 70.60%. To further analyze the differentially expressed genes (DEGs), the samples were grouped into four pairs, of which the pair of 40MA and 40MB at the early microspore stage showed the most DEGs (5100 members). According to the abnormal appearance of the tapetum cells in 40MA, a series of tapetum development related genes were screened and analyzed. In addition, a total of 623 genes with differential expressions in the tetrad stage, but not in the early microspore stage between the two lines were filtered as the microspore abortion initiation related candidates. Twelve genes were selected to validate the sequencing result by quantitative RT-PCR. In this study, we identified a number of candidate genes involved in the initiation of microspore degeneration, which may provide a new perspective to unravel the molecular mechanism of Ogura CMS.


Asunto(s)
Perfilación de la Expresión Génica/métodos , Proteínas de Plantas/genética , Raphanus/fisiología , Flores/genética , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Secuenciación de Nucleótidos de Alto Rendimiento , Infertilidad Vegetal , Polen/genética , Polen/fisiología , Raphanus/genética , Análisis de Secuencia de ARN , Secuenciación del Exoma
11.
Proc Natl Acad Sci U S A ; 104(52): 20826-31, 2007 Dec 26.
Artículo en Inglés | MEDLINE | ID: mdl-18087040

RESUMEN

Inactivation of the transcription factor p53 is central to carcinogenesis. Yet only approximately one-half of cancers have p53 loss-of-function mutations. Here, we demonstrate a mechanism for p53 inactivation by apoptosis repressor with caspase recruitment domain (ARC), a protein induced in multiple cancer cells. The direct binding in the nucleus of ARC to the p53 tetramerization domain inhibits p53 tetramerization. This exposes a nuclear export signal in p53, triggering Crm1-dependent relocation of p53 to the cytoplasm. Knockdown of endogenous ARC in breast cancer cells results in spontaneous tetramerization of endogenous p53, accumulation of p53 in the nucleus, and activation of endogenous p53 target genes. In primary human breast cancers with nuclear ARC, p53 is almost always WT. Conversely, nearly all breast cancers with mutant p53 lack nuclear ARC. We conclude that nuclear ARC is induced in cancer cells and negatively regulates p53.


Asunto(s)
Neoplasias de la Mama/embriología , Caspasas/metabolismo , Proteínas del Citoesqueleto/fisiología , Regulación Neoplásica de la Expresión Génica , Proteínas del Tejido Nervioso/fisiología , Proteína p53 Supresora de Tumor/fisiología , Transporte Activo de Núcleo Celular , Línea Celular Tumoral , Proteínas del Citoesqueleto/metabolismo , Dimerización , Humanos , Carioferinas/metabolismo , Modelos Biológicos , Mutación , Proteínas del Tejido Nervioso/metabolismo , Estructura Terciaria de Proteína , ARN Interferente Pequeño/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Transcripción Genética , Proteína p53 Supresora de Tumor/química , Proteína Exportina 1
12.
Environ Pollut ; 247: 180-187, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30677662

RESUMEN

Hydrotalcite (HT) is a layered double hydroxide (LDH), which is considered as a potential adsorbent to remove anion contaminants. In this study, adsorption of chromate (CrO4) and phosphate (PO4) on HT was conducted at various pH and temperatures. Related adsorption mechanisms were determined via the isotherm, kinetic, and competitive adsorption studies as well as the Cr K-edge X-ray absorption fine-structure (XAFS) spectroscopy. The maximum adsorption capacities for CrO4 and PO4 on HT were 0.16 and 0.23 mmol g-1. Regarding adsorption kinetics, CrO4 and PO4 adsorption on HT could be well described by the second order model, and the rate coefficient of CrO4 and PO4 on HT decreased significantly with the increasing pH from 5 to 9. The adsorption kinetics for CrO4 and PO4 were divided into fast and slow stages with the boundary at 15 min. This biphasic adsorption behavior might be partially attributed to multiple reactive pathways including anion exchange and surface complexation. Fitting results of Cr K-edge EXAFS analysis showed a direct bonding between CrO4 and Al on HT surfaces. Such a surface complexation appeared to be the rate-limiting step for CrO4 adsorption on HT. By contrast, the diffusion through the hydrated interlayer space of HT was the major rate-limiting step for PO4. This study determined the adsorption behaviors of CrO4 and PO4 on HT, including the initial transfer process and the subsequent adsorption mechanisms. Such information could improve the strategy to use HT as the potential adsorbent for the remediation of anionic pollutants.


Asunto(s)
Hidróxido de Aluminio/química , Cromatos/química , Hidróxido de Magnesio/química , Modelos Químicos , Fosfatos/química , Adsorción , Hidróxidos , Cinética , Espectroscopía de Absorción de Rayos X
13.
3 Biotech ; 8(7): 311, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-30003000

RESUMEN

High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small RNA libraries and one degradome library. A total of 518 known and 976 novel miRNAs were identified, of which, 338 known and 18 novel miRNAs were expressed in all six libraries, respectively. A total of 52 known and 57 novel miRNAs were identified to be significantly differentially expressed between RE and RL, and 195 mRNAs were verified to be the targets of 194 miRNAs by degradome sequencing. According to the degradome analysis, 11 differentially expressed miRNAs had miRNA-mRNA targets, and 13 targets were identified for these 11 miRNAs. Of the 13 miRNA-mRNA targets, 4 genes (RSG11079.t1, RSG11844.t1, RSG16775.t1, and RSG42419.t1) were involved in hormone-mediated signaling pathway, 2 gens (RSG11079.t1 and RSG16775.t1) were related to post-embryonic root development, and 1 gene (RSG23799.t1) was involved in anatomical structure morphogenesis, according to the GO function analysis for biological process. Target Genes participated in these processes are important candidates for further studies. This study provides valuable information for a better understanding of the molecular mechanisms involved in radish tuberous root formation and development.

14.
Sci Rep ; 7: 46545, 2017 05 09.
Artículo en Inglés | MEDLINE | ID: mdl-28484233

RESUMEN

Mercury contamination in food can pose serious health risks to consumers and coal-fired power plants have been identified as the major source of mercury emissions. To assess the current state of mercury pollution in food crops grown near coal-fired power plants, we measured the total mercury concentration in vegetables and grain crops collected from farms located near two coal-fired power plants. We found that 79% of vegetable samples and 67% of grain samples exceeded the PTWI's food safety standards. The mercury concentrations of soil samples were negatively correlated with distances from the studied coal-fired power plants, and the mercury contents in lettuce, amaranth, water spinach, cowpea and rice samples were correlated with the mercury contents in soil samples, respectively. Also, the mercury concentrations in vegetable leaves were much higher than those in roots and the mercury content of vegetable leaves decreased significantly after water rinses. Our calculation suggests that probable weekly intake of mercury for local residents, assuming all of their vegetables and grains are from their own farmland, may exceed the toxicologically tolerable values allowed, and therefore long-term consumptions of these contaminated vegetables and grains may pose serious health risks.


Asunto(s)
Carbón Mineral , Mercurio/análisis , Oryza/química , Centrales Eléctricas , Contaminantes del Suelo/análisis , Suelo/química , Verduras/química , Vigna/química , Oryza/crecimiento & desarrollo , Hojas de la Planta/química , Verduras/crecimiento & desarrollo , Vigna/crecimiento & desarrollo
15.
Pharmacol Rep ; 69(6): 1254-1262, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-29128807

RESUMEN

BACKGROUND: Apigenin is a flavonoid compound, widely distributed in natural plants. Various studies have suggested that apigenin has inhibitory effects towards several drug transporters, such as the organic anion transporting (OAT) polypeptides, 1B1 and 1B3 (OATP1B1 and OATP1B3). However, the mechanism by which apigenin interacts with OAT1 has not been well studied. METHODS: MDCK cells stably-expressing OAT1 were used to examine the inhibitory effects of apigenin on OAT1. UPLC-MS/MS was used to evaluate the in vitro and in vivo effects of apigenin on the uptake of acyclovir by OAT1. Cytotoxicity was determined by the cell viability, MTT assays. RESULTS: Apigenin effectively inhibited the activity of OAT1 in a dose-dependent manner with an IC50 value of 0.737µM. Pre-incubation of cells with apigenin caused a time-dependent inhibition (TDI) of OAT1. Additionally, we examined the interactions between apigenin and acyclovir or adefovir. Data showed that apigenin (1µM) significantly blocked the uptake of acyclovir by OAT1 in vitro with an inhibition rate of 55%. In vivo, apigenin could increase the concentration of acyclovir in plasma when co-administered with acyclovir. Importantly, the MTT assays showed that, at a dose of 50µM, apigenin significantly reduced the cytotoxicity of adefovir and substantially increased cell viability from 50.6% to 112.62%. CONCLUSION: Our results demonstrate that apigenin regulates OAT1, and can cause TDI or herb-drug interaction (HDI) when used in combination with acyclovir or adefovir. Therefore, apigenin could be used as a nephroprotective agent when used in combination with the substrates of OAT1.


Asunto(s)
Apigenina/farmacología , Interacciones de Hierba-Droga , Enfermedades Renales/prevención & control , Proteína 1 de Transporte de Anión Orgánico/antagonistas & inhibidores , Aciclovir/farmacocinética , Aciclovir/toxicidad , Adenina/análogos & derivados , Adenina/farmacocinética , Adenina/toxicidad , Animales , Antivirales/farmacocinética , Antivirales/toxicidad , Apigenina/administración & dosificación , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Perros , Relación Dosis-Respuesta a Droga , Concentración 50 Inhibidora , Enfermedades Renales/inducido químicamente , Células de Riñón Canino Madin Darby , Masculino , Proteína 1 de Transporte de Anión Orgánico/metabolismo , Organofosfonatos/farmacocinética , Organofosfonatos/toxicidad , Ratas , Ratas Sprague-Dawley , Espectrometría de Masas en Tándem , Factores de Tiempo
16.
Sci Rep ; 7: 40369, 2017 01 10.
Artículo en Inglés | MEDLINE | ID: mdl-28071744

RESUMEN

The incorporation of tendon graft into bone tunnel is one of the most challenging clinical issues in anterior cruciate ligament (ACL) reconstruction. As a biodegradable metal, Mg has appropriate mechanical strength and osteoinductive effects, thus may be a promising alternative to commercialized products used for graft fixation. Therefore, it was hypothesized that Mg based interference screws would promote tendon graft-bone junction healing when compared to Ti screws. Herein, we compared the effects of Mg and Ti screws on tendon graft healing in rabbits with ACL reconstruction via histological, HR-pQCT and mechanical analysis. The histological results indicated that Mg screws significantly improved the graft healing quality via promoting mineralization at the tendon graft enthesis. Besides, Mg screws significantly promoted bone formation in the peri-screw region at the early healing stage. Importantly, Mg screws exhibited excellent corrosion resistance and the degradation of Mg screws did not induce bone tunnel widening. In tensile testing, there were no significant differences in the load to failure, stress, stiffness and absorption energy between Mg and Ti groups due to the failure mode at the midsubstance. Our findings demonstrate that Mg screws can promote tendon graft healing after ACL reconstruction, implying a potential alternative to Ti screws for clinical applications.


Asunto(s)
Lesiones del Ligamento Cruzado Anterior/cirugía , Ligamento Cruzado Anterior/cirugía , Magnesio/uso terapéutico , Osteogénesis/efectos de los fármacos , Animales , Ligamento Cruzado Anterior/crecimiento & desarrollo , Ligamento Cruzado Anterior/fisiopatología , Lesiones del Ligamento Cruzado Anterior/fisiopatología , Reconstrucción del Ligamento Cruzado Anterior/métodos , Materiales Biocompatibles/uso terapéutico , Tornillos Óseos , Humanos , Osteogénesis/fisiología , Conejos , Titanio/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos
17.
Pharmacol Rep ; 69(5): 1103-1112, 2017 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-28988709

RESUMEN

BACKGROUND: Selective inhibitors of human urate transporter 1 (hURAT1) are considered to be effective treatment for hyperuricemia and gout, which can reduce the reabsorption of more than 90% of uric acid in the proximal tubule of the kidney. We aimed to design and synthesize a more potent hURAT1 based on the structure of Lesinurad (LU), which was reported to lower uric acid levels with IC50 value of hURAT1 (about 60µM). METHODS: A cell model was conducted and characterized via Real-time qRCR and Western blot. We synthesized and identified a new midazole analogue of LU. Cells stably-expressing hURAT1 or human organic anion transporter 1 (hOAT1) were used in the [14C] urate or 6-carboxyfluorescein (6-CF) uptake assays to test the activities of the newly synthesized compound. The uric acid lowering effects of LU and LUM and their effects on urea nitrogen and creatinine in potassium oxonate-induced hyperuricemic rats were analyzed. RESULTS: The [14C] Urate uptake assay using hURAT1 stably transfected MDCK cells indicated that LUM was more potent than LU against hURAT1, with IC50 values of 3.22µM and 65.47µM, respectively. LU and LUM also effectively suppressed hOAT1-mediated 6-CF uptake, and the IC50 hURAT1/IC50 hOAT1 of LU and LUM was1.49 and 0.35 respectively, indicating a better selectivity for LUM than LU. In vivo, LUM-Na (40mg/kg) showed more potent activity in reducing serum uric acid levels in potassium oxonate-induced hyperuricemic rats, compared to similar doses of LU-Na. CONCLUSION: LUM was demonstrated to be as potent a uricosuric drug as LU.


Asunto(s)
Transportadores de Anión Orgánico/antagonistas & inhibidores , Proteínas de Transporte de Catión Orgánico/antagonistas & inhibidores , Tioglicolatos/farmacología , Triazoles/farmacología , Animales , Nitrógeno de la Urea Sanguínea , Línea Celular , Supervivencia Celular/efectos de los fármacos , Creatinina/sangre , Perros , Humanos , Hiperuricemia/tratamiento farmacológico , Estructura Molecular , Transportadores de Anión Orgánico/genética , Transportadores de Anión Orgánico/metabolismo , Proteínas de Transporte de Catión Orgánico/genética , Proteínas de Transporte de Catión Orgánico/metabolismo , Ratas , Tioglicolatos/química , Triazoles/química
18.
Brain Res ; 1110(1): 159-65, 2006 Sep 19.
Artículo en Inglés | MEDLINE | ID: mdl-16887106

RESUMEN

Fragile X syndrome, the most common form of inherited mental retardation, is caused by the absence of the FMR-1 gene product FMRP. In addition to the hallmark cognitive defect, other symptoms are also apparent including hyperactivity, seizures and sensory abnormalities including a characteristic increase in sensitivity to auditory, tactile, visual, and olfactory stimuli. Fragile X is a developmental disorder with the first symptoms apparent in the first year of life but little is known about the role of FMRP in developmental processes. The sensory hyperreactivity of fragile X can be reproduced in fmr-1 knockout (KO) mice evident as abnormal audiogenic startle response and increased audiogenic seizure susceptibility. Here, we studied the onset and emergence of the startle deficit in fmr-1 KO mice during development. The startle response was first detectable at the end of the 2nd postnatal week in wild-type mice. The amplitude of startle response showed a substantial increase until the 4th postnatal week followed by a further but moderate increase up to adulthood. Expression of the fmr1 gene was detectable in the startle circuit before the onset and throughout the development of the startle response. Although the onset and amplitude of the startle response were not altered in fmr1 KO mice until the 3rd-4th postnatal week, beyond this age it failed to develop further resulting in an overall response deficit in adult KO mice. This indicates that although Fmrp is dispensable at the initial steps of startle response development, it is necessary for the full development of the response.


Asunto(s)
Vías Auditivas/crecimiento & desarrollo , Período Crítico Psicológico , Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil/fisiología , Reflejo de Sobresalto/fisiología , Estimulación Acústica/métodos , Factores de Edad , Análisis de Varianza , Animales , Vías Auditivas/anatomía & histología , Vías Auditivas/metabolismo , Conducta Animal , Relación Dosis-Respuesta en la Radiación , Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil/genética , Hibridación in Situ/métodos , Ratones , Ratones Noqueados , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reflejo de Sobresalto/efectos de la radiación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos
19.
J Bone Miner Res ; 30(11): 2044-57, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25917347

RESUMEN

Vascular hyperpermeability and highly upregulated bone resorption in the destructive repair progress of steroid-associated osteonecrosis (SAON) are associated with a high expression of VEGF and high Src activity (Src is encoded by the cellular sarcoma [c-src] gene). This study was designed to prove our hypothesis that blocking the VEGF-Src signaling pathway by specific Src siRNA is able to prevent destructive repair in a SAON rabbit model. Destructive repair in SAON was induced in rabbits. At 2, 4, and 6 weeks after SAON induction, VEGF, anti-VEGF, Src siRNA, Src siRNA+VEGF, control siRNA, and saline were introduced via intramedullary injection into proximal femora for each group, respectively. Vascularization and permeability were quantified by dynamic contrast-enhanced (DCE) MRI. At week 6 after SAON induction, proximal femurs were dissected for micro-computed tomography (µCT)-based trabecular architecture with finite element analysis (FEA), µCT-based angiography, and histological analysis. Histological evaluation revealed that VEGF enhanced destructive repair, whereas anti-VEGF prevented destructive repair and Src siRNA and Src siRNA+VEGF prevented destructive repair and enhanced reparative osteogenesis. Findings of angiography and histomorphometry were consistent with those determined by DCE MRI. Src siRNA inhibited VEGF-mediated vascular hyperpermeability but preserved VEGF-induced neovascularization. Bone resorption was enhanced in the VEGF group and inhibited in the anti-VEGF, Src siRNA, Src siRNA+VEGF groups as determined by both 3D µCT and 2D histomorphometry. FEA showed higher estimated failure load in the Src siRNA and Src siRNA+VEGF groups when compared to the vehicle control group. Blockage of VEGF-Src signaling pathway by specific Src siRNA was able to prevent steroid-associated destructive repair while improving reconstructive repair in SAON, which might become a novel therapeutic strategy.


Asunto(s)
Osteonecrosis/inducido químicamente , Osteonecrosis/enzimología , ARN Interferente Pequeño/metabolismo , Esteroides/efectos adversos , Cicatrización de Heridas , Familia-src Quinasas/antagonistas & inhibidores , Animales , Modelos Animales de Enfermedad , Análisis de Elementos Finitos , Técnicas de Silenciamiento del Gen , Silenciador del Gen , Masculino , Modelos Biológicos , Osteogénesis , Osteonecrosis/diagnóstico por imagen , Osteonecrosis/patología , Perfusión , Conejos , Microtomografía por Rayos X , Familia-src Quinasas/metabolismo
20.
Thyroid ; 22(6): 575-84, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22471242

RESUMEN

BACKGROUND: Papillary thyroid carcinoma (PTC) patients presenting with cervical lymph nodes (LN) metastases (M) have a variable outcome. The objective of this study is to assess the value of meticulous histopathologic examination and genotyping in stratifying these patients into clinically relevant prognostic subgroups. METHODS: This was a retrospective clinical and histopathological review of PTC patients with lymph node metastases at presentation identified between 1980 and 2002 in a single institution. Primary tumors from patients who later recurred were matched to a group of patients who did not recur and subjected to mass spectrometry genotyping encompassing the most significant oncogenes in thyroid carcinomas. RESULTS: There were 246 patients who satisfied the inclusion criteria. The median follow-up was 10.8 years. The presence of >3 metastatic nodes was an independent predictor of decreased recurrence free survival (p=0.03). In patients <45 years, none of 45 with 1-2 metastatic LN recurred, including 26 patients followed for a median of 13 years without radioactive iodine (RAI) therapy. BRAF mutations were found in 28 (78%) of 36 genotyped tumors. Combined positivity for BRAF and extra-nodal extension was much stronger in predicting disease specific survival (DSS) (p=0.004) than the single analysis of BRAF (p=0.12) or extra-nodal extension (p=0.02). CONCLUSIONS: (i) The number of metastatic LN is an independent predictor of recurrence in all age groups and identifies a subset of young patients with excellent prognosis who may not benefit from RAI therapy. (ii) Combined positivity for BRAF and extra-nodal extension has additive prognostic value in predicting DSS. (iii) Classification systems that assign the same magnitude of risk for recurrence or death to all patients with N1 disease should be revisited.


Asunto(s)
Ganglios Linfáticos/patología , Mutación/genética , Proteínas Proto-Oncogénicas B-raf/genética , Neoplasias de la Tiroides/genética , Neoplasias de la Tiroides/patología , Carcinoma , Carcinoma Papilar , Femenino , Estudios de Seguimiento , Genotipo , Humanos , Radioisótopos de Yodo , Metástasis Linfática , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia/epidemiología , Valor Predictivo de las Pruebas , Pronóstico , Estudios Retrospectivos , Tasa de Supervivencia , Cáncer Papilar Tiroideo , Neoplasias de la Tiroides/diagnóstico
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