1.
J Clin Microbiol
; 49(10): 3691-3, 2011 Oct.
Artículo
en Inglés
| MEDLINE
| ID: mdl-21813723
RESUMEN
We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.