Transcription factor LBD16 targets cell wall modification/ion transport genes in peach lateral root formation.

LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKEs (LBDs/ASLs) are plant-specific transcription factors that function downstream of auxin-regulated lateral root (LR) formation. Our previous research found that PpLBD16 positively regulates peach (Prunus persica) LR formation. However, the downstream regulatory network and target genes of PpLBD16 are still largely unknown. Here, we constructed a PpLBD16 homologous overexpression line and a PpLBD16 silenced line. We found that overexpressing PpLBD16 promoted peach root initiation, while silencing PpLBD16 inhibited peach root formation. Through RNA sequencing (RNA-seq) analysis of roots from PpLBD16 overexpression and silenced lines, we discovered that genes positively regulated by PpLBD16 were closely related to cell wall synthesis and degradation, ion/substance transport, and ion binding and homeostasis. To further detect the binding motifs and potential target genes of PpLBD16, we performed DNA-affinity purification sequencing (DAP-seq) analysis in vitro. PpLBD16 preferentially bound to CCNGAAANNNNGG (MEME-1), [C/T]TTCT[C/T][T/C] (MEME-2), and GCGGCGG (ABR1) motifs. By combined analysis of RNA-seq and DAP-seq data, we screened candidate target genes for PpLBD16. We demonstrated that PpLBD16 bound and activated the cell wall modification-related genes EXPANSIN-B2 (PpEXPB2) and SUBTILISIN-LIKE PROTEASE 1.7 (PpSBT1.7), the ion transport-related gene CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1 (PpCNGC1) and the polyphenol oxidase (PPO)-encoding gene PpPPO, thereby controlling peach root organogenesis and promoting LR formation. Moreover, our results displayed that PpLBD16 and its target genes are involved in peach LR primordia development. Overall, this work reveals the downstream regulatory network and target genes of PpLBD16, providing insights into the molecular network of LBD16-mediated LR development.

Silicon enhances the drought resistance of peach seedlings by regulating hormone, amino acid, and sugar metabolism.

BACKGROUND: Drought is one of the main concerns worldwide and restricts the development of agriculture. Silicon improves the drought resistance of plants, but the underlying mechanism remains unclear. RESULTS: We sequenced the transcriptomes of both control and silicon-treated peach seedlings under drought stress to identify genes or gene networks that could be managed to increase the drought tolerance of peach seedlings. Peach (Prunus persica) seedlings were used to analyse the effects of silicon on plant growth and physiological indexes related to drought resistance under drought stress. The results showed that silicon addition improved the water use efficiency, antioxidant capacity, and net photosynthetic rate, inhibition of stomatal closure, promoted the development of roots, and further regulated the synthesis of hormones, amino acids and sugars in peach seedlings. A comparative transcriptome analysis identified a total of 2275 genes that respond to silicon under drought stress. These genes were mainly involved in ion transport, hormone and signal transduction, biosynthetic and metabolic processes, stress and defence responses and other processes. We analysed the effects of silicon on the modulation of stress-related hormonal crosstalk and amino acid and sugar metabolism. The results showed that silicon promotes zeatin, gibberellin, and auxin biosynthesis, inhibits the synthesis of abscisic acid, then promote lateral root development and inhibit stomatal closure, and regulates the signal transduction of auxin, cytokinin, gibberellin and salicylic acid. Silicon also regulates the metabolism of various amino acids and promotes the accumulation of sucrose and glucose to improve drought resistance of peach seedlings. CONCLUSIONS: Silicon enhanced the drought resistance of peach seedlings by regulating stress-related hormone synthesis and signal transduction, and regulating amino acid and sugar metabolism.

Phosphatidylcholine Enhances Homeostasis in Peach Seedling Cell Membrane and Increases Its Salt Stress Tolerance by Phosphatidic Acid.

Salt stress is a major adverse abiotic factor seriously affecting fruit tree growth and development. It ultimately lowers fruit quality and reduces yield. Phosphatidylcholine (PC) is an important cell membrane component that is critical for cell structure and membrane stability maintenance. In this study, we found that the addition of external PC sources significantly increased the tolerance of one-year-old peach trees, Prunus persica (L.) Batsch., to salt stress and attenuated their damage. The effect of exogenous application of 200 mg/L PC exerted the most significant positive effect. Its use caused seedling leaf stomatal opening, contributing to normal gas exchange. Moreover, beneficial effects were exerted also to the root system, which grew normally under salt stress. Meanwhile, phospholipase D activity in the cell was promoted. The production of phosphatidic acid (PA) was enhanced by increased decomposition of phospholipids; PA serves as a secondary messenger involved in plant biological process regulation and the reduction in the reactive oxygen species- and peroxide-induced damage caused by salt stress. The possible mechanism of action is via promoted plant osmotic regulation and tolerance to salt stress, reducing salt stress-induced injury to plants.

Silicon inhibits gummosis in peach via ethylene and PpERF-PpPG1 pathway.

Silicon (Si) is abundant in nature, and it has been proved to be beneficial for the healthy growth and development of many plant species, improve plant stress resistance. Gummosis in peach is an invasive disease that causes widespread and serious damage. Mechanical damage and ethylene (ETH) can induce gummosis in peach shoots in the field. In this research, we found that Si as a chemical substance or signal to enhance plant resistance can reduce the synthesis of ETH, thereby inhibiting gummosis in peach. The results showed that Si can decrease the rate of gummosis, reduce the expression level of PpACS1 (1-aminocyclopropane -1-carboxylate synthase gene) and reduce the enzyme activity of polygalacturonase (PG). It was further discovered that Si can regulate the gene expression of PpERF21 and PpERF27. Yeast one-hybrid and dual-luciferase reporter assays showed that PpERF21 and PpERF27, through direct interaction with the promoter of PpPG1, inhibited the transcriptional activation of PpPG1. Overexpression of PpERF21 and PpERF27 effectively reduced fruit colloid production when bacterial cells harbouring the expression vector were used to instantaneously infect peach fruit. These results show that Si can inhibit the synthesis of ETH and mediate PpERF21 and PpERF27 expression to inhibit the expression of PpPG1, thereby inhibiting gummosis in peach.

Silicon inhibits gummosis by promoting polyamine synthesis and repressing ethylene biosynthesis in peach.

Silicon is a beneficial element for plant growth, as well as for improving plant resistance to multiple biotic and abiotic stresses. Gummosis is a common harmful disease in peach and is induced by many factors. However, the effect of silicon on gummosis of peach has not been determined yet. In this study, we reported that application of silicon significantly reduced gummosis by regulating biosynthesis of ethylene and polyamines in peach. Ethylene promoted the development of gummosis by inducing the expression of genes encoding cell wall degrading enzymes. While application of different types of polyamines, including spermidine and spermine, dramatically inhibited the occurrence of gummosis. Moreover, polyamines inhibited the ethylene biosynthesis by down-regulating expression of ethylene biosynthetic gene PpACS1 (1-aminocyclopropane -1-carboxylic acid synthase), as well as the enzymatic activity of ACS. We further found that application of silicon significantly restricted the development of gummosis in peach. Exogenous silicon dramatically inhibited expression of PpACS1 and the enzymatic activity of its product to reduce ethylene biosynthesis. Simultaneously, the activity of S-adenosylmethionine decarboxylase, a key enzyme in ployamines biosynthesis, was increased by 9.85% under silicon treatment, resulting in elevated accumulation of polyamines. Thus, our data proved that application of silicon restricted gummosis development by activating ployamines biosynthesis and inhibiting ethylene synthesis in peach.