RESUMEN
Autism spectrum disorder (ASD) is a common neurodevelopmental condition characterized by marked genetic heterogeneity. Recent studies of rare structural and sequence variants have identified hundreds of loci involved in ASD, but our knowledge of the overall genetic architecture and the underlying pathophysiological mechanisms remains incomplete. Glycine receptors (GlyRs) are ligand-gated chloride channels that mediate inhibitory neurotransmission in the adult nervous system but exert an excitatory action in immature neurons. GlyRs containing the α2 subunit are highly expressed in the embryonic brain, where they promote cortical interneuron migration and the generation of excitatory projection neurons. We previously identified a rare microdeletion of the X-linked gene GLRA2, encoding the GlyR α2 subunit, in a boy with autism. The microdeletion removes the terminal exons of the gene (GLRA2(Δex8-9)). Here, we sequenced 400 males with ASD and identified one de novo missense mutation, p.R153Q, absent from controls. In vitro functional analysis demonstrated that the GLRA2(Δex8)(-)(9) protein failed to localize to the cell membrane, while the R153Q mutation impaired surface expression and markedly reduced sensitivity to glycine. Very recently, an additional de novo missense mutation (p.N136S) was reported in a boy with ASD, and we show that this mutation also reduced cell-surface expression and glycine sensitivity. Targeted glra2 knockdown in zebrafish induced severe axon-branching defects, rescued by injection of wild type but not GLRA2(Δex8-9) or R153Q transcripts, providing further evidence for their loss-of-function effect. Glra2 knockout mice exhibited deficits in object recognition memory and impaired long-term potentiation in the prefrontal cortex. Taken together, these results implicate GLRA2 in non-syndromic ASD, unveil a novel role for GLRA2 in synaptic plasticity and learning and memory, and link altered glycinergic signaling to social and cognitive impairments.
Asunto(s)
Glicina/metabolismo , Receptores de Glicina/genética , Receptores de Glicina/metabolismo , Adolescente , Adulto , Animales , Trastorno del Espectro Autista/metabolismo , Trastorno Autístico/metabolismo , Niño , Preescolar , Glicina/genética , Humanos , Potenciación a Largo Plazo/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos C57BL , Neurogénesis/efectos de los fármacos , Plasticidad Neuronal/efectos de los fármacos , Neuronas/metabolismo , Transducción de Señal/efectos de los fármacos , Transmisión Sináptica/fisiología , Pez CebraRESUMEN
Interactions between genetic and environmental factors, like exposure to stress, have an important role in the pathogenesis of mood-related psychiatric disorders, such as major depressive disorder. The polyspecific organic cation transporters (OCTs) were shown previously to be sensitive to the stress hormone corticosterone in vitro, suggesting that these transporters might have a physiologic role in the response to stress. Here, we report that OCT2 is expressed in several stress-related circuits in the brain and along the hypothalamic-pituitary-adrenocortical (HPA) axis. Genetic deletion of OCT2 in mice enhanced hormonal response to acute stress and impaired HPA function without altering adrenal sensitivity to adrenocorticotropic hormone (ACTH). As a consequence, OCT2(-/-) mice were potently more sensitive to the action of unpredictable chronic mild stress (UCMS) on depression-related behaviors involving self-care, spatial memory, social interaction and stress-sensitive spontaneous behavior. The functional state of the glycogen synthase kinase-3ß (GSK3ß) signaling pathway, highly responsive to acute stress, was altered in the hippocampus of OCT2(-/-) mice. In vivo pharmacology and western blot experiments argue for increased serotonin tonus as a main mechanism for impaired GSK3ß signaling in OCT2(-/-) mice brain during acute response to stress. Our findings identify OCT2 as an important determinant of the response to stress in the brain, suggesting that in humans OCT2 mutations or blockade by certain therapeutic drugs could interfere with HPA axis function and enhance vulnerability to repeated adverse events leading to stress-related disorders.
Asunto(s)
Encéfalo/fisiopatología , Glucógeno Sintasa Quinasa 3/metabolismo , Proteínas de Transporte de Catión Orgánico/metabolismo , Estrés Psicológico/metabolismo , Potenciales de Acción/efectos de los fármacos , Enfermedad Aguda , Animales , Encéfalo/efectos de los fármacos , Enfermedad Crónica , Corticosterona/administración & dosificación , Corticosterona/metabolismo , Depresión/metabolismo , Glucógeno Sintasa Quinasa 3 beta , Hormonas/administración & dosificación , Hormonas/metabolismo , Masculino , Ratones Endogámicos C57BL , Ratones Noqueados , Norepinefrina/metabolismo , Proteínas de Transporte de Catión Orgánico/genética , Transportador 2 de Cátion Orgánico , Proteínas Proto-Oncogénicas c-akt/metabolismo , Células Piramidales/efectos de los fármacos , Células Piramidales/fisiología , Resiliencia Psicológica , Serotonina/metabolismo , IncertidumbreRESUMEN
High-affinity transporters for norepinephrine (NE) and serotonin (5-HT), which ensure neurotransmitter clearance at the synapse, are the principal targets of widely used antidepressant drugs. Antidepressants targeting these high-affinity transporters, however, do not provide positive treatment outcomes for all patients. Other monoamine transport systems, with lower affinity, have been detected in the brain, but their role is largely unknown. Here we report that OCT2, a member of the polyspecific organic cation transporter (OCT) family, is expressed notably in the limbic system and implicated in anxiety and depression-related behaviors in the mouse. Genetic deletion of OCT2 in mice produced a significant reduction in brain tissue concentrations of NE and 5-HT and in ex vivo uptake of both these neurotransmitters in the presence of the dual 5-HT-NE transport blocker, venlafaxine. In vivo clearance of NE and 5-HT evaluated using microiontophoretic electrophysiology was diminished in the hippocampus of OCT2(-/-) mice in the presence of venlafaxine, thereby affecting postsynaptic neuronal activity. OCT2(-/-) mice displayed an altered sensitivity to acute treatments with NE- and/or 5-HT-selective transport blockers in the forced-swim test. Moreover, the mutant mice were insensitive to long-term venlafaxine treatment in a more realistic, corticosterone-induced, chronic depression model. Our findings identify OCT2 as an important postsynaptic determinant of aminergic tonus and mood-related behaviors and a potential pharmacological target for mood disorders therapy.
Asunto(s)
Antidepresivos de Segunda Generación/uso terapéutico , Encéfalo/metabolismo , Ciclohexanoles/uso terapéutico , Depresión/tratamiento farmacológico , Norepinefrina/metabolismo , Proteínas de Transporte de Catión Orgánico/fisiología , Serotonina/metabolismo , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Animales , Antidepresivos de Segunda Generación/farmacología , Ansiedad/tratamiento farmacológico , Encéfalo/efectos de los fármacos , Corticosterona , Ciclohexanoles/farmacología , Depresión/inducido químicamente , Modelos Animales de Enfermedad , Femenino , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Hipocampo/fisiología , Sistema Límbico/efectos de los fármacos , Sistema Límbico/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Imagen Molecular/métodos , Imagen Molecular/psicología , Proteínas de Transporte de Catión Orgánico/biosíntesis , Proteínas de Transporte de Catión Orgánico/genética , Transportador 2 de Cátion Orgánico , Ensayo de Unión Radioligante/métodos , Ensayo de Unión Radioligante/psicología , Clorhidrato de VenlafaxinaRESUMEN
Lack of muscle glycogen phosphorylase activity leads to McArdle's disease, a rare metabolic myopathy. To investigate its molecular basis at the nucleic acid level, we isolated muscle phosphorylase cDNA clones from a human cDNA library in Escherichia coli plasmid pBR 322. Subcloning of one insertion of M13 bacteriophage permitted its definite identification by sequencing. Northern blot experiments revealed one specific messenger RNA of 3.4 kilobases found uniquely in tissues expressing muscle phosphorylase. We show that McArdle's disease exhibits a molecular heterogeneity at the messenger RNA level. In eight unrelated cases of McArdle's disease in which no inactive proteins had been detected, we assayed muscle biopsies for phosphorylase mRNA by Northern blotting. In five cases, no muscle phosphorylase mRNA could be detected, while in three other cases, normal length mRNA was present in lower amounts. Moreover, Southern blot analysis of DNA isolated from white blood cells in four McArdle patients revealed no major deletion or rearrangements of the phosphorylase gene as compared with controls.
Asunto(s)
Enfermedad del Almacenamiento de Glucógeno Tipo V/genética , Enfermedad del Almacenamiento de Glucógeno/genética , Fosforilasas/deficiencia , Secuencia de Bases , Clonación Molecular , ADN/genética , Regulación de la Expresión Génica , Genes , Humanos , Músculos/enzimología , Músculos/fisiología , Fosforilasas/genética , ARN Mensajero/genéticaRESUMEN
Neurotransmitter transporters play an important role in the control of synaptic transmission by ensuring the clearance of transmitters liberated in the synaptic cleft. In the case of monoaminergic neurotransmitters, this clearance is carried out by high-affinity reuptake transporters located in the plasma membrane of the presynaptic terminals. Recently plasma membrane monoamine transporter (PMAT), a transporter from the SLC29 (equilibrative nucleoside transporter) family, was shown to transport in vitro monoaminergic neurotransmitters, in particular dopamine and serotonin, nearly as efficiently as the high-affinity transporters. This transporter, well expressed in CNS, represents an interesting candidate for the control and modulation of aminergic pathways. We performed an extensive study of the distribution of PMAT in the rat brain. Our results highlight PMAT expression in brain regions which play a pivotal role in significant CNS functions and human neuropathologies. Using in situ hybridization immunohistochemistry co-labeling, PMAT mRNA was found in various neuron subtypes, including glutamatergic neurons of the hippocampus, mitral cells of the olfactory bulbs and GABAergic neurons in the substantia nigra pars reticulata and hypothalamus. Paradoxically, rat PMAT mRNA was found in some but not all monoaminergic nuclei. It was on the contrary predominantly expressed in major cholinergic groups throughout the brain, including brainstem motor nuclei, components of the basal forebrain cholinergic system and cholinergic interneurons of the striatum. These systems, implicated in locomotion, associative and spatial memory and reward-related learning, are disrupted at early stages of Parkinson's and Alzheimer's disease. Taken together, our observations support a role for PMAT in monoamine uptake in cholinergic neurons.
Asunto(s)
Encéfalo/metabolismo , Proteínas de Transporte de Membrana/metabolismo , Neurotransmisores/metabolismo , Animales , Encéfalo/citología , Colina O-Acetiltransferasa/metabolismo , Glutamato Descarboxilasa/metabolismo , Histidina Descarboxilasa/metabolismo , Inmunohistoquímica/métodos , Hibridación in Situ/métodos , Neuronas/metabolismo , ARN Mensajero/metabolismo , Ratas , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
We undertook cloning and sequencing of the 5' portion of the human aldolase A gene to elucidate the mechanisms that govern synthesis of its different mRNAs. The sequenced gene is the only active gene in human-rodent fibroblastic somatic hybrids, while the other aldolase A-related sequences are inactive. S1 mapping and primer extension analysis enabled us to demonstrate that three promoter regions were implicated in the initiation of different aldolase A mRNAs, differing only in their 5' non-coding extremities. A distal promoter, N (non-specific), governs the synthesis of a 5' non-coding region of 142 bases composed of two exons, N1 and N2, which are found in a variety of tissues. A median promoter, M (muscle), is only active in skeletal muscle, and initiates the transcription by a 5' non-coding exon of 45 bases. Finally, a proximal promoter, H (housekeeping), contained in a "G + C-rich island", permits transcription of three colinear mRNAs containing 172, 126 or 112 bases of 5' non-coding sequence; their expression seems ubiquitous. These three promoters are arranged in 1.5 X 10(3) base-pairs of DNA. Homologies between rat and human genomic sequences and the absence of homology between promoters or 5' non-coding exons of the same species exclude a recent duplication of the promoter regions.
Asunto(s)
Fructosa-Bifosfato Aldolasa/genética , Regiones Promotoras Genéticas , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Genes , Humanos , Células Híbridas , Datos de Secuencia Molecular , ARN Mensajero/genética , Ratas , Homología de Secuencia de Ácido NucleicoRESUMEN
Antisera were raised in rabbits against native and sodium dodecylsulfate denatured forms of human acid alpha-glucosidase and beta-hexosaminidases A and B. Anti-native enzyme antisera were able to precipitate all or nearly all enzyme activity from cell extracts, and to eliminate all stainable activity on electrophoresis. Antisera prepared against denatured enzymes precipitated only a minor part of enzyme activity. Electrophoretic analysis showed that these antisera were able to bind to the enzyme molecule. The result was a slowing down of the anodic migration but not immobilization. The use of variants with hexosaminidase deficiencies helped to clarify the action of the antisera on the various hexosaminidase isozymes.
Asunto(s)
Anticuerpos/inmunología , Glucosidasas/inmunología , Hexosaminidasas/inmunología , alfa-Glucosidasas/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Electroforesis de las Proteínas Sanguíneas/métodos , Femenino , Humanos , Inmunoelectroforesis/métodos , Extractos Placentarios/inmunología , Embarazo , Desnaturalización Proteica , Conejos , beta-N-AcetilhexosaminidasasRESUMEN
In a previous study, we compared the alpha-mannosidase from mannosidosis tissues to that from normal one and we characterized the mutant. In this work, we show that the mutant inactivation by dialysis is reversible in different conditions and we investigate the nature of mannosidosis reactivating factors. The results obtained on pathological tissue by dialysis and by addition of dialysis fluid (DF), pronase treated DF, amino acid mixture, bivalent ions: Ca++, Zn++, Mg++, Co++ DF containing EDTA or DF heated to 600 degrees C suggest the reactivating factor includes both peptides and ions.
Asunto(s)
Errores Innatos del Metabolismo de los Carbohidratos/metabolismo , Reactivadores Enzimáticos/análisis , Manosa/metabolismo , Manosidasas/deficiencia , Errores Innatos del Metabolismo de los Carbohidratos/genética , Cationes/análisis , Fenómenos Químicos , Química , Diálisis , Ácido Edético/análisis , Femenino , Feto/metabolismo , Humanos , Hígado/enzimología , Mutación , Embarazo , alfa-ManosidasaRESUMEN
The human aldolase A gene is transcribed from three distinct promoters, the two ubiquitous promoters PN and PH and the muscle specific promoter PM. In the present study, we investigate further aldolase A mRNA structure and expression. We demonstrate that the upstream N-type exon is, in fact, extremely heterogeneous. RNAse H mapping experiments permit quantification of relative abundance of N, M, and H type mRNAs and show that the level of transcripts containing the downstream H-type exon is at least 30 times higher than that of those containing N exon, in all tissues tested. Aldolase A level is up-regulated in proliferating cells. Here we show that both N and H type mRNAs, although barely detectable in normal liver, are highly expressed in human hepatomas biopsies. Furthermore, in human lymphocytes, N-type mRNA level is enhanced by serum treatment, while in cultured Hep G2 cells, both N-type and H-type mRNA levels are increased by serum and by the tumor promoting agent PMA. Using CAT constructs in transfection experiments, we demonstrate that the H exon plus its upstream region can function autonomously: the 420 base pairs upstream of the H exon are sufficient to confer to promoter PH an efficiency comparable that of the complete SV40 early promoter and enhancer in two cell lines.
Asunto(s)
División Celular , Fructosa-Bifosfato Aldolasa/genética , Regiones Promotoras Genéticas , Secuencia de Bases , Northern Blotting , Carcinoma Hepatocelular/química , Cloranfenicol O-Acetiltransferasa/genética , Electroforesis en Gel de Agar , Endorribonucleasas , Humanos , Hígado/química , Neoplasias Hepáticas/química , Linfocitos/química , Datos de Secuencia Molecular , ARN Mensajero/genética , Ribonucleasa H , TransfecciónRESUMEN
The gene that is defective in patients with Duchenne and Becker muscular dystrophy consists of about 60 short exons scattered along a gigantic DNA region that spans some 2 megabase pairs. The encoded protein, dystrophin, was recently characterized as a component of muscle intracellular membranes of low abundance. The dystrophin messenger RNA is difficult to study in both normal and pathological tissue specimens because it is large (14 kilobases) and scarce (0.01-0.001% of total muscle mRNA). We report here that efficient in vitro co-amplifications of the mRNAs of the dystrophin gene and of a reporter gene, aldolase A, by the polymerase chain reaction procedure enables us to obtain a quantitative estimate of the dystrophin gene transcript. A processed, transcribed segment was thus detected in 13 different human tissues. It ranged from 0.02-0.12% of total mRNA in skeletal muscle to 25,000 times less in lymphoblastoid cells.
Asunto(s)
Proteínas Musculares/biosíntesis , Músculos/análisis , Distrofias Musculares/genética , ARN Mensajero/biosíntesis , Química Encefálica , Línea Celular , Distrofina , Humanos , Linfocitos/análisis , Proteínas Musculares/genética , Transcripción Genética , Células Tumorales Cultivadas/análisis , Vísceras/análisisRESUMEN
The activity of seven lysosomal enzymes was determined in 25 lymphoblastoid cell lines. These lines included normal controls transformed with Epstein-Barr virus, Burkitt's lymphomas and other lymphomas with or without EBV genome. Four lines were deficient in total beta-hexosaminidase activity. The deficiency was as severe as that of the variant O (Sandhoff's disease) of clinical beta-hexosaminidase deficiency. The electrophoretic pattern was also similar to that observed in Sandhoff's disease. The possible mechanisms explaining the high frequency of beta-hexosaminidase deficiency in lymphoblastoid cell lines are discussed.
Asunto(s)
Hexosaminidasas/deficiencia , Linfocitos/enzimología , Linfoma/enzimología , Complejo Antígeno-Anticuerpo , Linfoma de Burkitt/enzimología , Línea Celular , Transformación Celular Neoplásica , Herpesvirus Humano 4/genética , Humanos , Sueros Inmunes , beta-N-AcetilhexosaminidasasRESUMEN
Hexosaminidases, lysosomal enzymes whose deficiency is responsible for several genetic disorders, exist as two major forms: form A, containing two types of subunits alpha and beta; and form B, containing only beta subunits. We have used a technique involving successively electrophoresis of denatured proteins, transfer (blotting) onto nitrocellulose, and labelling by appropriate antibodies raised against the dissociated forms of hexosaminidases A and B. This technique allows the detection of alpha and beta subunits in crude extracts of normal tissues. The presence of beta chains was demonstrated in the liver of a fetus affected with Sandhoff's disease, deficient in functional hexosaminidases A and B.
Asunto(s)
Hexosaminidasas/análisis , Enfermedad de Sandhoff/enzimología , Electroforesis , Femenino , Hexosaminidasas/inmunología , Humanos , Sueros Inmunes , MasculinoRESUMEN
cDNA clones for rat muscle-type creatine kinase and glycogen phosphorylase and aldolase A were isolated from a rat muscle cDNA library. An additional clone recognizing an unidentified 2.7-kilobase pair mRNA species was also isolated. These cDNA clones were used as probes to investigate the expression of the corresponding mRNAs during muscle development. Two aldolase A mRNA species were detected, one of 1650 bases expressed in non-muscle tissues, fetal muscle, and adult slow-twitch muscle, the other of 1550 bases was highly specific of adult fast-twitch skeletal muscle differentiation. These aldolase A mRNAs were shown by primer extension to differ by their 5' ends. The accumulation of muscle-type phosphorylase and creatine kinase and muscle-specific aldolase A mRNA accumulation during muscle development seems to be a coordinate process occurring progressively from the 17th day of intrauterine life up to the 30th day after birth. In contrast, the 2.7-kilobase pair RNA species is maximally expressed at the 1st week after birth as is the neonatal form of myosin heavy chain mRNA.
Asunto(s)
Feto/metabolismo , Fructosa-Bifosfato Aldolasa/metabolismo , Regulación de la Expresión Génica , Desarrollo de Músculos , Fosforilasas/genética , ARN Mensajero/metabolismo , Envejecimiento , Animales , ADN/genética , ADN/aislamiento & purificación , Músculos/embriología , Músculos/enzimología , Hibridación de Ácido Nucleico , Poli A/metabolismo , ARN/metabolismo , RatasRESUMEN
Previous electrophysiological and pharmacological studies on central and peripheral glia revealed the presence of voltage-gated Na channels with properties that are similar but not identical to those of neuronal Na channels. Here we report the isolation and characterization of a cDNA encoding the C-terminal portion of a putative glial Na-channel (Na-G) alpha subunit. The amino acid sequence deduced from this cDNA indicates that the Na-G represents a separate molecular class within the mammalian Na-channel multigene family. By Northern blot, RNase protection, and in situ hybridization assays, we demonstrate that, in addition to brain astroglia, the Na-G mRNA is expressed in cultures of Schwann cells derived from dorsal root ganglia or from sciatic nerve. In vivo, the Na-G mRNA is detected not only in brain, dorsal root ganglia, and sciatic nerve, but also in tissues outside the nervous system including cardiac and skeletal muscle and lung. Its level varies according to the tissue and is developmentally regulated. The sequence and expression data concur in designating Na-G as an distinct type of Na channel, presumably with low sensitivity to tetrodotoxin.
Asunto(s)
Astrocitos/fisiología , Encéfalo/fisiología , ARN Mensajero/genética , Canales de Sodio/genética , Transcripción Genética , Secuencia de Aminoácidos , Animales , Animales Recién Nacidos , Northern Blotting , Línea Celular , Células Cultivadas , ADN/genética , Expresión Génica , Datos de Secuencia Molecular , Desnervación Muscular , Músculos/inervación , Músculos/fisiología , Especificidad de Órganos , ARN/genética , ARN/aislamiento & purificación , ARN Mensajero/metabolismo , Ratas , Ratas Endogámicas , Células de Schwann/fisiología , Nervio Ciático/fisiología , Homología de Secuencia de Ácido NucleicoRESUMEN
The Na-G ion channel, previously cloned from a rat astroglia cDNA library, belongs to a new family of ion channels, related to but distinct from the predominant brain and muscle fast voltage-gated Na(+) channels. In vivo, the corresponding transcripts are widely expressed in peripheral nervous system neurons and glia, but only in selected subpopulations of neuronal and glia-like cells of the central nervous system. In the present report, we show that Na-G messenger RNA level in astrocyte and Schwann cell cultures is modulated in a cell-specific manner by several growth factors, hormones, and intracellular second messengers pathways. Striking changes in transcript level were observed in the two types of glia in response to protein-kinase A activation and to treatment with the neuregulin glial growth factor, indicating regulation of the Na-G gene by neuroglial signaling. By transient transfection of Na-G/reporter constructs into cultured cells, we show that a short genomic region, encompassing the first exon and 375 bp upstream, bears a high glial-specific transcriptional activity while part of the first intron behaves as a negative regulatory element. In vivo footprinting experiments revealed binding of glial-specific nuclear factors to several sites of the Na-G promoter region. Finally, Na-G/reporter constructs are shown to sustain a low but reproducible transcriptional response to cAMP, accounting in part for the elevation in mRNA level elicited by cAMP in Schwann cells and its reduction in astrocytes.
Asunto(s)
Astrocitos/fisiología , Proteínas del Tejido Nervioso/genética , Células de Schwann/fisiología , Canales de Sodio/genética , 8-Bromo Monofosfato de Adenosina Cíclica/farmacología , Animales , Astrocitos/citología , Secuencia de Bases , Células Cultivadas , Corteza Cerebral/citología , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Huella de ADN , Desoxirribonucleasa I , Dexametasona/farmacología , Exones , Regulación de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica/fisiología , Glucocorticoides/farmacología , Intrones , Datos de Secuencia Molecular , Regiones Promotoras Genéticas/fisiología , ARN Mensajero/análisis , Ratas , Células de Schwann/citología , Transcripción Genética/efectos de los fármacos , Transcripción Genética/fisiología , Transfección , Canales de Sodio Activados por VoltajeRESUMEN
Na-G is a putative sodium (or cationic) channel expressed in neurons and glia of the PNS, in restricted neuronal subpopulations of the brain, and in several tissues outside the nervous system, like lung and adrenal medulla. To analyze the mechanisms underlying tissue-specific expression of this channel, we isolated the 5' region of the corresponding gene and show that Na-G mRNA transcription proceeds from a single promoter with multiple initiation sites. By transgenic mice studies, we demonstrate that 600 bp containing the Na-G proximal promoter region and the first exon are sufficient to drive the expression of a beta-galactosidase reporter gene in neurons of both CNS and PNS, whereas expression in Schwann cells depends on more remote DNA elements lying in the region between -6,500 and -1,050 bp upstream of the main transcription initiation sites. Crucial elements for lung-specific expression seem to be located in the region between -1,050 and -375 bp upstream of the promoter. Using in vivo footprint experiments, we demonstrate that several sites of the Na-G proximal promoter region are bound specifically by nuclear proteins in dorsal root ganglion neurons, as compared with nonexpressing hepatoma cells.