RESUMEN
BACKGROUND: Depressive symptoms may affect female mid-life sexuality, whereas sexual problems tend to aggravate depression. Despite this, data assessing this association drawn from mid-aged Paraguayan women are scarce. OBJECTIVE: This study aimed to assess the association between depressed mood and the risk of sexual dysfunction during female mid-life. METHODS: Sexually active urban-living women from Asunción, Paraguay (n = 193, aged 40-60 years) were surveyed with the 6-item Female Sexual Function Index (FSFI-6), the 10-item Center for Epidemiological Studies Depression Scale (CESD-10), and a general questionnaire containing personal and partner information. Depressed mood was defined as a total CESD-10 score of 10 or more, and an increased risk for sexual dysfunction as an FSFI-6 total score of 19 or less. The association of depressed mood and an increased risk of sexual dysfunction was evaluated with multivariable Poisson regression. RESULTS: The mean age (±standard deviation) of surveyed woman was 48.3 ± 6.0 years and 61.1% (n = 118) were perimenopausal and postmenopausal. A total of 21.8% (n = 42) had depressed mood and 28.5% (n = 55) had an increased risk of sexual dysfunction. The final adjusted regression model determined that women with depressed mood were twice as likely to have an increased risk of sexual dysfunction, compared to women with normal mood (adjusted prevalence ratio = 2.14, 95% confidence interval 1.26-3.60). On the other hand, depressed mood was associated with a mean total FSFI-6 score that was 20% lower than that observed among women with normal mood (adjusted incidence rate ratio = 0.80, 95% confidence interval 0.68-0.93). CONCLUSION: In this mid-aged Paraguayan female sample there was a significant association between depressed mood and an increased risk of sexual dysfunction.
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Depresión/complicaciones , Conducta Sexual/psicología , Disfunciones Sexuales Fisiológicas/epidemiología , Disfunciones Sexuales Psicológicas/epidemiología , Adulto , Femenino , Humanos , Persona de Mediana Edad , Paraguay/epidemiología , Perimenopausia/psicología , Posmenopausia/psicología , Prevalencia , Análisis de Regresión , Disfunciones Sexuales Fisiológicas/psicología , Disfunciones Sexuales Psicológicas/psicología , Población Urbana/estadística & datos numéricosRESUMEN
BACKGROUND: Fetal growth restriction is, despite advances in neonatal care and uptake of antenatal ultrasound scanning, still a major cause of perinatal morbidity. Neonates with birth weight > 10th percentile are assumed to be appropriate-for-gestational-age (AGA), although many are at increased risk of perinatal morbidity, because of undetected mild restriction of growth potential. We hypothesized that within AGA neonates, reduced fetal growth velocities are associated with adverse neonatal outcome. METHODS: A retrospective cohort study of singleton pregnancies, in the Maastricht University Medical Centre (MUMC) between 2010 and 2016. Women had two fetal biometry scans (18-22 weeks and 30-34 weeks of gestational age) and delivered a newborn with a birth weight between the 10th-80th percentile. Differences in growth velocities of the abdominal circumference (AC), biparietal diameter (BPD), head circumference (HC) and femur length (FL) were compared between the suboptimal AGA (sAGA) (birth weight centiles 10-50) and optimal AGA (oAGA) (birth weight centiles 50-80) group. We assessed the association between velocities and neonatal outcomes. RESULTS: We included 934 singleton pregnancies. In the suboptimal AGA group, fetal growth velocities were lower (in mm/week): AC 10.72 ± 1.00 vs 11.23 ± 1.00 (p < .001), HC 10.50 ± 0.80 vs 10.68 ± 0.77 (p = 0.001), BPD 3.01 ± 0.28 vs 3.08 ± 0.27 (p < .0001) and FL 2.47 ± 0.21 vs 2.50 ± 0.22 (p = 0.014), compared to the optimal AGA group. Neonates with an adverse neonatal outcome had significantly lower growth velocities (in mm/week) of: AC 10.57 vs 10.94 (p = 0.034), HC 10.28 vs 10.59 (p = 0.003) and BPD 2.97 vs 3.04 (p = 0.043) compared to those with normal outcome. An inverse association was observed between the AC velocity and a composite adverse neonatal outcome (OR) = 0.667 (95%CI 0.507-0.879, p = 0.004), and between the AC velocity and neonates with NICU stay (OR) = 0.733 (95%CI 0.570-0.942, p = 0.015). Neonates with a birthweight lower than expected (based on the abdominal circumference at 20 weeks) had significantly more composite adverse neonatal outcomes 8.5% vs 5.0% (p = 0.047), NICU stays 9.6% vs 3.8% (p < .0001) and hospital stays 44.4% vs 35.6% (p = 0.006). CONCLUSIONS: Appropriate-for-gestational-age neonates are a heterogeneous group with some showing suboptimal fetal growth. Abnormal fetal growth velocities, especially abdominal circumference velocity, are associated with adverse neonatal outcome and can potentially improve the detection of mild growth restriction when used in multivariate models.
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Desarrollo Fetal , Retardo del Crecimiento Fetal/fisiopatología , Edad Gestacional , Enfermedades del Recién Nacido/etiología , Adulto , Biometría , Femenino , Humanos , Recién Nacido , Masculino , Embarazo , Estudios Retrospectivos , Ultrasonografía PrenatalRESUMEN
BackgroundGeneral anesthetics could protect key neurotransmitter systems, such as the dopaminergic system, from hypoxic-ischemic encephalopathy (HIE) by limiting excessive glutamatergic neurotransmission. However, anesthetics may adversely affect inflammation and tau phosphorylation.MethodsA near-term sheep model of HIE by umbilical cord occlusion (UCO) under anesthesia was used. The effect of propofol and isoflurane on the dopaminergic neurotransmitter phenotype in the substantia nigra (SN) was studied using tyrosine hydroxylase immunohistochemistry. The overall microglial response and tau phosphorylation were also measured in the SN, surrounding the midbrain gray matter structures and the hippocampal white matter.ResultsThe isoflurane-treated UCO group had fewer tyrosine hydroxylase-expressing neurons in the SN at 8 h after the insult than the propofol-treated UCO or sham-operated groups (P<0.05). The microglial response was unchanged in the SN region. In the thalamus and the hippocampal stratum moleculare layer, the propofol-treated UCO group had a lower microglial response than the corresponding sham-operated group. Both UCO and the use of anesthetics additively increased tau phosphorylation in the SN region, thalamus, and hippocampus.ConclusionThe choice of anesthetics is important for an emergency C-section. Propofol could potentially protect the dopaminergic neurotransmitter phenotype within the SN at the cost of a widespread increase in tau phosphorylation.
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Hipoxia-Isquemia Encefálica/metabolismo , Isoflurano/farmacología , Propofol/farmacología , Sustancia Negra/enzimología , Tirosina 3-Monooxigenasa/metabolismo , Proteínas tau/metabolismo , Anestésicos/farmacología , Animales , Animales Recién Nacidos , Mapeo Encefálico , Dopamina/farmacología , Neuronas Dopaminérgicas/efectos de los fármacos , Femenino , Glutamina/metabolismo , Hipocampo/metabolismo , Hipoxia/metabolismo , Inflamación , Masculino , Mesencéfalo/metabolismo , Microglía/metabolismo , Neurotransmisores/metabolismo , Fosforilación , Ovinos , Transmisión Sináptica , Cordón Umbilical/patologíaRESUMEN
Bronchopulmonary dysplasia (BPD) is a chronic lung disease occurring in very and extremely preterm infants undergoing mechanical ventilation. Given the altered lung vascular growth characterizing BPD, circulating angiogenic cells could be useful biomarkers to predict the risk. The objective of the study was to determine whether the percentages of circulating angiogenic cells (CD34+VEGFR-2+, CD34+CD133+VEGFR-2+, and CD45-CD34+CD133+VEGFR-2+ cells), assessed in the peripheral blood at birth by flow cytometry, could be used as markers for the risk of BPD. In one-hundred and forty-two preterm neonates (gestational age less than 32 weeks and/or birth weight less than 1500 g) admitted to our tertiary care Neonatal Intensive Care Unit between 2006 and 2009, we evaluated the percentages of circulating angiogenic cells at birth, at 7 days, and, in a subset of infants (n=40), at 28 days of life. The main outcome was the correlation between cell counts at birth and the subsequent risk of developing BPD. In our study, all the three cell populations failed to predict the development of BPD or other diseases of prematurity. We suggest that these cells cannot be used as biomarkers in preterm infants, and that research is needed to find other early predictors of BPD.
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Displasia Broncopulmonar/diagnóstico , Células Madre Hematopoyéticas , Recien Nacido Prematuro/sangre , Recién Nacido de muy Bajo Peso/sangre , Neovascularización Patológica , Antígeno AC133 , Antígenos CD/sangre , Antígenos CD34/sangre , Biomarcadores/sangre , Displasia Broncopulmonar/sangre , Displasia Broncopulmonar/patología , Citometría de Flujo , Edad Gestacional , Glicoproteínas/sangre , Células Madre Hematopoyéticas/metabolismo , Células Madre Hematopoyéticas/patología , Humanos , Antígenos Comunes de Leucocito/sangre , Recuento de Leucocitos , Péptidos/sangre , Fenotipo , Valor Predictivo de las Pruebas , Estudios Retrospectivos , Factores de Riesgo , Centros de Atención Terciaria , Receptor 2 de Factores de Crecimiento Endotelial Vascular/sangreRESUMEN
The developing lung and immune systems are very plastic and their developmental pathway can be influenced by various endogenous and/or exogenous factors. In the last years translational research with various animal models has been helpful to answer some basic questions about the effect of chorioamnionitis on maturation and development of the foetal lung and immune system. Chorioamnionitis can induce a cascade of lung injury, pulmonary inflammation and remodelling in the foetal lung. Chorioamnionitis-induced IL-1 production is consistently associated with lung maturation, induced by enhancing surfactant protein and lipid synthesis. IL-1 therefore seems to be the main link between lung inflammation and lung maturation, which largely prevents RDS in preterm infants. On the other hand, chorioamnionitis can also cause structural lung changes and affect the expression of growth factors, like TGF-ß, CTGF, FGF-10 or BMP-4, which are crucial for branching morphogenesis. These changes result in alveolar and microvascular simplification similar to BPD. Neonatal outcome may also be affected by chorioamnionitis by modulating the efficacy of the immune system. Chorioamnionitis can induce LPS-tolerance (endotoxin hyporesponsiveness/immunoparalysis), which may prevent further foetal lung damage but increases susceptibility to postnatal infections. The inflammatory and developmental signalling pathways affected by chorioamnionitis form delicately regulated networks, which interact with each other to control lung development. In addition to chorioamnionitis, these pathways can be affected by other prenatal (steroid) or postnatal factors (mechanical ventilation, oxygen exposure, infection, steroids). Because the postnatal response to injury appears to be highly dependent on prenatal exposures, the "secondary hit" hypothesis is very plausible, in which exposure to chorioamnionitis is a predisposition for the development of adverse neonatal outcomes.
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Corioamnionitis/inmunología , Citocinas/inmunología , Inflamación/embriología , Inflamación/inmunología , Pulmón/embriología , Pulmón/inmunología , Femenino , Humanos , EmbarazoRESUMEN
Chorioamnionitis frequently induces a fetal inflammatory response syndrome (FIRS), characterized by an elevation of proinflammatory mediators and systemic inflammation. Although there is increasing evidence that inflammation and lipid metabolism influence each other, the effects of chorioamnionitis-induced FIRS on fetal lipid homeostasis are currently not known. Accordingly, we hypothesize that chorioamnionitis induces an inflammatory response in the fetal liver, consequently leading to metabolic disturbances. Chorioamnionitis was induced by intra-amniotic injection of 10 mg endotoxin (control) for 2 d or 2 wk before delivery. Saline injections were given to controls. The effect of chorioamnionitis on hepatic inflammation and metabolic parameters was analyzed in ovine fetuses at the GA of 125 d (normal GA = 150 d). We found that 2 d after the endotoxin injections, inflammatory markers were significantly higher compared with controls. In addition, lipid and glucose metabolism were disturbed in response to endotoxin. Moreover, the antioxidant state capacity was reduced, and hepatic damage was apparent. Two weeks after the endotoxin injections, the fetal livers were still inflamed and had higher glucose concentrations in the blood. In addition, the levels of markers for hepatic damage (alanine aminotransferase and aspartate aminotransferase) were increased. In conclusion, chorioamnionitis induces liver inflammation leading to metabolic disturbances in the fetus.
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Corioamnionitis/fisiopatología , Feto/metabolismo , Feto/patología , Inflamación/patología , Inflamación/fisiopatología , Metabolismo de los Lípidos , Hígado/patología , Animales , Corioamnionitis/inducido químicamente , Endotoxinas/farmacología , Femenino , Hematopoyesis , Interleucina-8/genética , Interleucina-8/metabolismo , Hígado/fisiopatología , Embarazo , OvinosRESUMEN
BACKGROUND: Hypoxic-ischaemic encephalopathy is associated with development of cerebral palsy and cognitive disability later in life and is therefore one of the fundamental problems in perinatal medicine. The xanthine-oxidase inhibitor allopurinol reduces the formation of free radicals, thereby limiting the amount of hypoxia-reperfusion damage. In case of suspected intra-uterine hypoxia, both animal and human studies suggest that maternal administration of allopurinol immediately prior to delivery reduces hypoxic-ischaemic encephalopathy. METHODS/DESIGN: The proposed trial is a randomized double blind placebo controlled multicenter study in pregnant women at term in whom the foetus is suspected of intra-uterine hypoxia.Allopurinol 500 mg IV or placebo will be administered antenatally to the pregnant woman when foetal hypoxia is suspected. Foetal distress is being diagnosed by the clinician as an abnormal or non-reassuring foetal heart rate trace, preferably accompanied by either significant ST-wave abnormalities (as detected by the STAN-monitor) or an abnormal foetal blood scalp sampling (pH < 7.20).Primary outcome measures are the amount of S100B (a marker for brain tissue damage) and the severity of oxidative stress (measured by isoprostane, neuroprostane, non protein bound iron and hypoxanthine), both measured in umbilical cord blood. Secondary outcome measures are neonatal mortality, serious composite neonatal morbidity and long-term neurological outcome. Furthermore pharmacokinetics and pharmacodynamics will be investigated.We expect an inclusion of 220 patients (110 per group) to be feasible in an inclusion period of two years. Given a suspected mean value of S100B of 1.05 ug/L (SD 0.37 ug/L) in the placebo group this trial has a power of 90% (alpha 0.05) to detect a mean value of S100B of 0.89 ug/L (SD 0.37 ug/L) in the 'allopurinol-treated' group (z-test2-sided). Analysis will be by intention to treat and it allows for one interim analysis. DISCUSSION: In this trial we aim to answer the question whether antenatal allopurinol administration reduces hypoxic-ischaemic encephalopathy in neonates exposed to foetal hypoxia. TRIAL REGISTRATION NUMBER: Clinical Trials, protocol registration system: NCT00189007.
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Alopurinol/uso terapéutico , Asfixia Neonatal/prevención & control , Hipoxia Fetal/prevención & control , Depuradores de Radicales Libres/uso terapéutico , Hipoxia-Isquemia Encefálica/prevención & control , Atención Prenatal/métodos , Asfixia Neonatal/sangre , Asfixia Neonatal/complicaciones , Asfixia Neonatal/epidemiología , Biomarcadores/sangre , Método Doble Ciego , Femenino , Hipoxia Fetal/sangre , Hipoxia Fetal/complicaciones , Humanos , Hipoxia-Isquemia Encefálica/sangre , Hipoxia-Isquemia Encefálica/etiología , Recién Nacido , Análisis Multivariante , Factores de Crecimiento Nervioso/sangre , Países Bajos/epidemiología , Fosfopiruvato Hidratasa/sangre , Proyectos Piloto , Embarazo , Estudios Prospectivos , Análisis de Regresión , Subunidad beta de la Proteína de Unión al Calcio S100 , Proteínas S100/sangre , Xantina Oxidasa/antagonistas & inhibidoresRESUMEN
OBJECTIVE: We quantified the impact of chorioamnionitis on both the white and gray matter structures of the preterm ovine central nervous system (CNS). STUDY DESIGN: The CNS was studied at 125 days of gestation, either 2 or 14 days after the intraamniotic administration of 10 mg of lipopolysaccharide (LPS) (Escherichia coli) or saline. Apoptotic cells and cell types were analyzed in the brain, cerebellum, and spinal cord using flow cytometry. RESULTS: Apoptosis and microglial activation increased in all regions with prolonged exposure to LPS-induced chorioamnionitis. Astrocytes were increased in the brain and cerebellum of LPS-exposed fetuses but not in the spinal cord. Mature oligodendrocytes decreased in the cerebral and cerebellar white matter, the cerebral cortex, caudate putamen, and hippocampus 14 days after LPS. Neurons in the cerebral cortex, hippocampus, and substantia nigra were reduced 14 days after LPS. CONCLUSION: Fetal inflammation globally but differentially affected the CNS depending on the maturational stage of the brain region.
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Encéfalo/patología , Corioamnionitis , Médula Espinal/patología , Amnios , Animales , Cerebelo/patología , Corioamnionitis/etiología , Femenino , Inyecciones , Lipopolisacáridos/administración & dosificación , Embarazo , Ovinos , Factores de TiempoRESUMEN
The aim of the present study was to determine the effects of fetal asphyxia (FA) on anxiety and serotonergic neurons in young adult and middle-aged rats. FA was induced at embryonic day 17 by clamping the uterine circulation for 75 min. Anxiety-related behavior was tested in an open field, and design-based stereology was used for counting serotonergic (5-hydroxytryptamine/serotonin, 5-HT) neurons in the dorsal raphe nucleus (DRN). The open field revealed increased anxiety in the 19-month-old FA rats in comparison to control animals. No significant differences were found in DRN 5-HT neuron numbers at 6 months. At 19 months, however, FA significantly lowered the mean density and volume of 5-HT neurons in the DRN as compared to controls. Further, an age-related reduction was found in the total number, the mean density and the mean volume of 5-HT neurons within the FA group. In conclusion, FA is associated with increased anxiety and age-related changes in 5-HT immunohistochemistry within the DRN. These results support the notion that insults caused by asphyxiation during critical periods of brain development could create a predisposition to serotonergic abnormalities and anxiety deficits in adulthood.
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Hipoxia Fetal/fisiopatología , Neuronas/metabolismo , Núcleos del Rafe/citología , Serotonina/metabolismo , Envejecimiento/fisiología , Animales , Ansiedad/fisiopatología , Conducta Animal/fisiología , Femenino , Masculino , Neuronas/citología , Ratas , Ratas Endogámicas LewRESUMEN
INTRODUCTION: The age of diagnosis of Huntington's disease (HD) varies among individuals with the same HTT CAG-repeat expansion size. We investigated whether early-life events, like perinatal insults or neurodevelopmental disorders, influence the diagnosis age. METHODS: We used data from 13,856 participants from REGISTRY and Enroll-HD, two large international multicenter observational studies. Disease-free survival analyses of mutation carriers with an HTT CAG repeat expansion size above and including 36 were computed through Kaplan-Meier estimates of median time until an HD diagnosis. Comparisons between groups were computed using a Cox proportional hazard survival model adjusted for CAG-repeat expansion length. We also assessed whether the group effect depended on gender and the affected parent. RESULTS: Insults in the perinatal period were associated with an earlier median age of diagnosis of 45.00 years (95%CI: 42.07-47.92) compared to 51.00 years (95%CI: 50.68-51.31) in the reference group, with a CAG-adjusted hazard ratio of 1.61 (95%CI: 1.26-2.06). Neurodevelopmental disorders were also associated with an earlier median age of diagnosis than the reference group of 47.00 years (95% CI: 43.38-50.62) with a CAG-adjusted hazard ratio of 1.42 (95%CI: 1.16-1.75). These associations did not change significantly with gender or affected parent. CONCLUSIONS: These results, derived from large observational datasets, show that perinatal insults and neurodevelopmental disorders are associated with earlier ages of diagnosis of magnitudes similar to the effects of known genetic modifiers of HD. Given their clear temporal separation, these early events may be causative of earlier HD onset, but further research is needed to prove causation.
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Enfermedad de Huntington/diagnóstico , Enfermedad de Huntington/etiología , Trastornos del Neurodesarrollo/complicaciones , Efectos Tardíos de la Exposición Prenatal/fisiopatología , Factores de Edad , Edad de Inicio , Estudios de Cohortes , Supervivencia sin Enfermedad , Femenino , Edad Gestacional , Humanos , Proteína Huntingtina/genética , Enfermedad de Huntington/genética , Cooperación Internacional , Masculino , Persona de Mediana Edad , Embarazo , Modelos de Riesgos Proporcionales , Sistema de Registros , Repeticiones de Trinucleótidos/genéticaRESUMEN
Hypoxic-ischemic encephalopathy remains a common cause of brain damage in neonates. Preterm infants have additional complications, as prematurity by itself increases the risk of encephalopathy. Currently, therapy for this subset of asphyxiated infants is limited to supportive care. There is an urgent need for therapies in preterm infants - and for representative animal models for preclinical drug development. In 1991, a novel rodent model of global asphyxia in the preterm infant was developed in Sweden. This method was based on the induction of asphyxia during the birth processes itself by submerging pups, still in the uterine horns, in a water bath followed by C-section. This insult occurs at a time-point when the rodent brain maturity resembles the brain of a 22-32 week old human fetus. This model has developed over the past 25 years as an established model of perinatal global asphyxia in the early preterm brain. Here we summarize the knowledge gained on the short- and long-term neuropathological and behavioral effects of asphyxia on the immature central nervous system.
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Asfixia , Encéfalo , Animales , Asfixia Neonatal , Femenino , Humanos , Hipoxia-Isquemia Encefálica , Recien Nacido Prematuro , Embarazo , RatasRESUMEN
Encephalopathy due to perinatal asphyxia (PA) is a major cause of neonatal morbidity and mortality in the period around birth. Preterm infants are especially at risk for cognitive, attention and motor impairments. Therapy for this subgroup is limited to supportive care, and new targets are thus urgently needed. Post-asphyxic excitotoxicity is partially mediated by excessive nitric oxide (NO) release. The aims of this study were to determine the timing and distribution of nitric oxide (NO) production after global PA in brain areas involved in motor regulation and coordination. This study focused on the rat striatum and cerebellum, as these areas also affect cognition or attention, in addition to their central role in motor control. NO/peroxynitrite levels were determined empirically with a fluorescent marker on postnatal days P5, P8 and P12. The distributions of neuronal NO synthase (nNOS), cyclic guanosine monophosphate (cGMP), astroglia and caspase-3 were determined with immunohistochemistry. Apoptosis was additionally assessed by measuring caspase-3-like activity from P2-P15. On P5 and P8, increased intensity of NO-associated fluorescence and cGMP immunoreactivity after PA was apparent in the striatum, but not in the cerebellum. No changes in nNOS immunoreactivity or astrocytes were observed. Modest changes in caspase-3-activity were observed between groups, but the overall time course of apoptosis over the first 11 days of life was similar between PA and controls. Altogether, these data suggest that PA increases NO/peroxynitrite levels during the first week after birth within the striatum, but not within the cerebellum, without marked astrogliosis. Therapeutic benefits of interventions that reduce endogenous NO production would likely be greater during this time frame.
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Asfixia Neonatal/metabolismo , Cerebelo/metabolismo , Cuerpo Estriado/metabolismo , Óxido Nítrico/metabolismo , Animales , Apoptosis/efectos de los fármacos , Astrocitos/metabolismo , Caspasa 3/metabolismo , GMP Cíclico/metabolismo , Femenino , Masculino , Óxido Nítrico Sintasa de Tipo I/metabolismo , Ácido Peroxinitroso/metabolismo , Periodo Posparto/metabolismo , Embarazo , RatasRESUMEN
The aim of our study was to investigate the effect of perinatal asphyxia on developmental apoptosis in the cervical and lumbar spinal cord in the neonatal rat. Perinatal asphyxia was induced by keeping pups at term in utero in a water bath at 37 degrees C for 20 min, followed by resuscitation. Effects of this treatment on developmental apoptosis were studied on postnatal days 2, 5 and 8 using terminal deoxynucleotidyl transferase (TdT)-dUTP-biotin nick end labelling (TUNEL) and caspase-3 staining. TUNEL positive cells were identified using double immunostaining. On postnatal day 2 an increase of 215% in TUNEL positive cells was detected (P=0.005) in laminae IV-VII of the lumbar spinal cord of rats which underwent perinatal asphyxia compared to controls. An increase of 55% compared to controls (P=0.03) was seen in laminae I-III of the lumbar spinal cord at postnatal day 8. TUNEL positive cells could be partly identified as microglia cells (ED1 positive) and oligodendrocytes (O4 positive). The effect of perinatal asphyxia on programmed cell death in the neonatal rat spinal cord was mainly observed in the intermediate zone and dorsal horn of the lumbar spinal cord. We conclude that perinatal asphyxia has a pronounced effect on the survival of cells in a specific region of the spinal cord and thus may have a profound effect on the development of motor networks.
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Apoptosis , Asfixia/complicaciones , Enfermedades de la Médula Espinal/etiología , Médula Espinal/crecimiento & desarrollo , Médula Espinal/patología , Animales , Animales Recién Nacidos , Asfixia/patología , Vértebras Cervicales , Femenino , Etiquetado Corte-Fin in Situ , Vértebras Lumbares , Masculino , Ratas , Ratas Wistar , Enfermedades de la Médula Espinal/patologíaRESUMEN
OBJECTIVES: To investigate the relationships between systemic and cerebral haemodynamics and oxygenation, and electroencephalogram (EEG) amplitude and frequency analysis studied by the cerebral function analyzing monitor (CFAM) during progressive hypovolemic hypotension. METHODS: Six piglets of 1 week of age, weighing 1.9-3.4 kg were mechanically ventilated under 1-1.5% halothane anaesthesia. After 1 h stabilization, blood was withdrawn in aliquots of 10 ml/kg over 15 min up to a total of 40-60 ml/kg. Arterial oxygenation was maintained at normal levels. Thereafter, the total blood volume previously withdrawn, was reinfused. Changes in near infrared spectroscopy (NIRS) parameters [cerebral oxidized cytochrome aa3 (Cytaa3), cerebral blood volume (CBV) or total haemoglobin (tHb: oxy- + deoxyhaemoglobin)], carotid blood flow (Q(car)), maximal EEG amplitude and EEG frequency percentages were analyzed continuously. RESULTS: The EEG amplitude remained stable until the mean arterial blood pressure (MAP), Q(car) and tHb dropped below 30 mmHg (41% of baseline), 20 ml/min (33% of baseline) and 82% of baseline, respectively. Delta (delta) wave frequency percentage of the CFAM increased significantly at MAP below 30 mm Hg. EEG amplitude remained depressed after blood reinfusion and haemodynamic recovery. Cytaa3 changes were not statistically significant, reflecting sufficient neuronal oxygenation. CONCLUSION: Our results show that electrocortical function is affected only by profound systemic hypotension. This occurred at a higher level of cerebral oxygen delivery than the level associated with neuronal hypoxia and secondary cell damage.
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Animales Recién Nacidos/fisiología , Circulación Cerebrovascular/fisiología , Electroencefalografía , Hipotensión/fisiopatología , Consumo de Oxígeno/fisiología , Animales , Análisis de los Gases de la Sangre , Glucemia/metabolismo , Corteza Cerebral/citología , Corteza Cerebral/fisiología , Femenino , Masculino , Neuronas/fisiología , Daño por Reperfusión/fisiopatología , PorcinosRESUMEN
OBJECTIVE: Neonates are commonly exposed to isolated hypoxemic episodes. In order to identify the risk of this, we correlated cerebral oxygen delivery and electrocortical brain activity during isolated graded and repetitive hypoxemia in 1-week-old piglets. METHODS: Six halothane-anesthetized piglets were subjected to two episodes of graded hypoxemia of 45 min duration. The fractional concentration of inspired oxygen (FiO(2)) was stepwise decreased at 15 min intervals from 0.21 to 0.15, 0.10 and 0.05. A second identical hypoxemic event was induced after 1 h of normoxemia (FiO(2) 0.21). Mean arterial pressure (MAP) and pH were maintained at baseline values during the whole experiment. We measured near infrared spectroscopy parameters (cerebral oxidized cytochrome aa(3) (Cytaa3), total hemoglobin (tHb: oxy- +deoxyhemoglobin)) corresponding to cerebral blood volume (CBV), carotid blood flow (Q(car)), intra-arterial oxygen saturation (SaO(2)), and mean maximal EEG amplitude and relative spectral power. RESULTS: Delta (delta) power increased significantly and the EEG amplitude dropped below 10 and 5 microV at the end of the first and the second hypoxemic period (PaO(2) 2.68+/-1.08 (P<0.05) and 2.87+/-0.58 kPa, respectively). Both EEG variables normalized during recovery (FiO(2) 0.21). Q(car), CBV and Cytaa3 were not changed. CONCLUSION: Acute isolated hypoxemia has to be sustained to induce neuronal hypofunction in normotensive animals. Hypoxic hypoxemia led to acute changes in neuronal activity, whereas cellular oxygenation remained unaffected.
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Animales Recién Nacidos/fisiología , Corteza Cerebral/metabolismo , Hipoxia/metabolismo , Oxígeno/metabolismo , Animales , Circulación Sanguínea , Análisis de los Gases de la Sangre/métodos , Presión Sanguínea , Dióxido de Carbono/metabolismo , Circulación Cerebrovascular , Citocromos/metabolismo , Electroencefalografía , Hemodinámica/fisiología , Hemoglobinas/metabolismo , Masculino , Flujo Sanguíneo Regional , Espectroscopía Infrarroja Corta/métodos , Porcinos , Factores de TiempoRESUMEN
Previous studies in rats have demonstrated that perinatal asphyxia (PA) produces long-term morphological alterations, particularly affecting hippocampus. neostriatum, and cerebral cortex. These changes were prevented by applying hypothermia during the asphyctic insult. Because these cerebral areas are involved in cognitive and motor functions, the aim of the present study was to determine whether periods of PA during normothermia or hypothermia produces long-term behavioral impairments in rats of both sexes. The cognitive and motor functions were studied using the spatial Morris water maze (MWM) task at 1.5 months, and the open field at 5 months, respectively. The present study revealed that female rats had a higher survival rate than males after PA in normothermic conditions (p < 0.014). and that hypothermia drastically prolonged the time of survival in both sexes (p < 0.001). There were no differences in learning and memory functions between groups or male and female rats when tested with MWM. Rats subjected to hypothermia treatment did not show differences in the MWM compared to controls. A lower locomotor activity in the open field test was only observed in male rats that suffered 15 and 20 min of PA in normothermia (p < 0.05). Hypothermia treatment prevented this hypoactivity. PA in females, even if severe, did not affect the motor activity. The data of both behavioral tests showed differences between sexes, i.e., the female rats learned the MWM task slower, and were more active in the open field. This work lends further support for the hypothesis that hypothermia can prevent mortality as well as long-term sequelae induced by PA.
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Asfixia Neonatal/fisiopatología , Daño Encefálico Crónico/fisiopatología , Hipotermia Inducida , Aprendizaje por Laberinto/fisiología , Actividad Motora/fisiología , Caracteres Sexuales , Sobrevida/fisiología , Animales , Encéfalo/fisiopatología , Femenino , Humanos , Recién Nacido , Masculino , Embarazo , Ratas , Ratas WistarRESUMEN
AIM: To evaluate the advantages, disadvantages, and short term morbidity and mortality of major surgical interventions performed in the neonatal intensive care unit. METHODS: A retrospective case review of 45 neonates was performed from April 1991 to September 1995. The characteristics of the patients were: gestational age 29 (SD 4) weeks (range 24 to 41 weeks); birth-weight 1305 (870) g (range 540 to 4040 g); presurgical weight 1430 (895) g (range 550 to 4370 g); postconceptional age at surgery 31 (4) weeks (26 to 47 weeks). The indications for surgery were: ligation of patent ductus arteriosus (n = 16); insertion of a subcutaneous ventricular catheter reservoir for hydrocephalus (n = 14); repair of congenital diaphragmatic hernia (n = 2); open lung biopsy (n = 1); and laparotomies (because of necrotising enterocolitis, anorectal malformations, and intestinal obstructions) (n = 12). The management of these neonates at laparotomy was: bowel resection with stomas (n = 8) and stomas (n = 4). No specially designed area was used to perform surgery. RESULTS: Local or systemic infection associated with surgery was not seen and no perioperative mortality was related to the surgical procedure. CONCLUSIONS: The neonatal intensive care unit is suitable for major surgery during the neonatal period and no special area is needed to perform complication free surgery.
Asunto(s)
Enfermedades del Recién Nacido/cirugía , Unidades de Cuidado Intensivo Neonatal , Estudios de Evaluación como Asunto , Humanos , Recién Nacido , Enfermedades del Recién Nacido/mortalidad , Infección de la Herida Quirúrgica , Cicatrización de HeridasRESUMEN
Large fetal neck masses can cause airway obstructions with potential fetal demise after delivery. The relationship of the neck mass to airway structures can be defined prenatally with ultrasound and magnetic resonance imaging (MRI). The ex utero intrapartum treatment (EXIT) procedure can be used to obtain a fetal airway while feto-maternal circulation is preserved to optimise fetal outcome. We present a case in which prenatally a large fetal neck mass was diagnosed on ultrasound and a successful EXIT procedure was performed. A review of the literature is given and the prenatal use of ultrasonography and MRI in case of fetal neck masses is discussed.
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Enfermedades Fetales/diagnóstico , Cuello/embriología , Teratoma/diagnóstico , Neoplasias de la Tiroides/diagnóstico , Adulto , Obstrucción de las Vías Aéreas/etiología , Obstrucción de las Vías Aéreas/prevención & control , Anestesia , Biopsia con Aguja , Cesárea , Femenino , Enfermedades Fetales/diagnóstico por imagen , Edad Gestacional , Humanos , Recién Nacido , Intubación Intratraqueal , Imagen por Resonancia Magnética , Cuello/diagnóstico por imagen , Embarazo , Teratoma/cirugía , Neoplasias de la Tiroides/cirugía , Ultrasonografía PrenatalRESUMEN
Although long-term monitoring of cerebral activity can be important in neonatal intensive care, the complexity of multi-channel EEG makes it less suitable for this purpose. In the past, a cerebral function monitor (CFM) was developed that analyses EEG. The output parameter of the CFM is a semi-logarithmic amplitude distribution resulting from the amplification, bandpass filtering, compression, rectification and smoothing of the single-channel EEG. Drawbacks of the CFM include its inflexibility and limited single-channel processing capacity and its lack of functionality for data storage, review or re-analysis. Modern computers are powerful enough that a system can be built which does not have these drawbacks. We have developed such a system: the Maastricht Cerebral Monitor (MCM). The MCM is a flexible system that not only overcomes the CFM drawbacks but also provides advanced signal analysis. It was developed with a software system (Poly) for acquisition, high quality real-time display, on-line analysis and storage of physiological signals. The MCM processes three EEG signals in the amplitude and frequency domains. Other parameters provided by the MCM are asymmetry of absolute frequency powers, percentage suppression, mean and 90% spectral edge frequency. Electrode impedance is recorded as a measure of quality of the recording.
Asunto(s)
Encéfalo/fisiología , Electroencefalografía/instrumentación , Cuidado Intensivo Neonatal , Monitoreo Fisiológico/instrumentación , Procesamiento de Señales Asistido por Computador/instrumentación , Calibración , Sistemas de Computación , Presentación de Datos , Impedancia Eléctrica , Electrodos , Diseño de Equipo , Humanos , Recién Nacido , Almacenamiento y Recuperación de la Información , Sistemas en Línea , Programas InformáticosRESUMEN
A female neonate developed abdominal distension with vomiting. She was suffering from meconium ileus and cystic fibrosis.