Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 10 de 10
Filtrar
1.
Nat Methods ; 20(6): 824-835, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37069271

RESUMEN

BigNeuron is an open community bench-testing platform with the goal of setting open standards for accurate and fast automatic neuron tracing. We gathered a diverse set of image volumes across several species that is representative of the data obtained in many neuroscience laboratories interested in neuron tracing. Here, we report generated gold standard manual annotations for a subset of the available imaging datasets and quantified tracing quality for 35 automatic tracing algorithms. The goal of generating such a hand-curated diverse dataset is to advance the development of tracing algorithms and enable generalizable benchmarking. Together with image quality features, we pooled the data in an interactive web application that enables users and developers to perform principal component analysis, t-distributed stochastic neighbor embedding, correlation and clustering, visualization of imaging and tracing data, and benchmarking of automatic tracing algorithms in user-defined data subsets. The image quality metrics explain most of the variance in the data, followed by neuromorphological features related to neuron size. We observed that diverse algorithms can provide complementary information to obtain accurate results and developed a method to iteratively combine methods and generate consensus reconstructions. The consensus trees obtained provide estimates of the neuron structure ground truth that typically outperform single algorithms in noisy datasets. However, specific algorithms may outperform the consensus tree strategy in specific imaging conditions. Finally, to aid users in predicting the most accurate automatic tracing results without manual annotations for comparison, we used support vector machine regression to predict reconstruction quality given an image volume and a set of automatic tracings.


Asunto(s)
Benchmarking , Microscopía , Microscopía/métodos , Imagenología Tridimensional/métodos , Neuronas/fisiología , Algoritmos
3.
BMC Bioinformatics ; 20(1): 50, 2019 Jan 24.
Artículo en Inglés | MEDLINE | ID: mdl-30678631

RESUMEN

BACKGROUND: The biomedical literature is expanding at ever-increasing rates, and it has become extremely challenging for researchers to keep abreast of new data and discoveries even in their own domains of expertise. We introduce PaperBot, a configurable, modular, open-source crawler to automatically find and efficiently index peer-reviewed publications based on periodic full-text searches across publisher web portals. RESULTS: PaperBot may operate stand-alone or it can be easily integrated with other software platforms and knowledge bases. Without user interactions, PaperBot retrieves and stores the bibliographic information (full reference, corresponding email contact, and full-text keyword hits) based on pre-set search logic from a wide range of sources including Elsevier, Wiley, Springer, PubMed/PubMedCentral, Nature, and Google Scholar. Although different publishing sites require different search configurations, the common interface of PaperBot unifies the process from the user perspective. Once saved, all information becomes web accessible allowing efficient triage of articles based on their actual relevance and seamless annotation of suitable metadata content. The platform allows the agile reconfiguration of all key details, such as the selection of search portals, keywords, and metadata dimensions. The tool also provides a one-click option for adding articles manually via digital object identifier or PubMed ID. The microservice architecture of PaperBot implements these capabilities as a loosely coupled collection of distinct modules devised to work separately, as a whole, or to be integrated with or replaced by additional software. All metadata is stored in a schema-less NoSQL database designed to scale efficiently in clusters by minimizing the impedance mismatch between relational model and in-memory data structures. CONCLUSIONS: As a testbed, we deployed PaperBot to help identify and manage peer-reviewed articles pertaining to digital reconstructions of neuronal morphology in support of the NeuroMorpho.Org data repository. PaperBot enabled the custom definition of both general and neuroscience-specific metadata dimensions, such as animal species, brain region, neuron type, and digital tracing system. Since deployment, PaperBot helped NeuroMorpho.Org more than quintuple the yearly volume of processed information while maintaining a stable personnel workforce.


Asunto(s)
Bases de Datos Bibliográficas , Internet , Metadatos , Publicaciones , Investigación Biomédica , Almacenamiento y Recuperación de la Información , Programas Informáticos , Interfaz Usuario-Computador
4.
BMC Bioinformatics ; 16: 216, 2015 Jul 10.
Artículo en Inglés | MEDLINE | ID: mdl-26156313

RESUMEN

BACKGROUND: The morphology of neurons offers many insights into developmental processes and signal processing. Numerous reports have focused on metrics at the level of individual branches or whole arbors; however, no studies have attempted to quantify repeated morphological patterns within neuronal trees. We introduce a novel sequential encoding of neurite branching suitable to explore topological patterns. RESULTS: Using all possible branching topologies for comparison we show that the relative abundance of short patterns of up to three bifurcations, together with overall tree size, effectively capture the local branching patterns of neurons. Dendrites and axons display broadly similar topological motifs (over-represented patterns) and anti-motifs (under-represented patterns), differing most in their proportions of bifurcations with one terminal branch and in select sub-sequences of three bifurcations. In addition, pyramidal apical dendrites reveal a distinct motif profile. CONCLUSIONS: The quantitative characterization of topological motifs in neuronal arbors provides a thorough description of local features and detailed boundaries for growth mechanisms and hypothesized computational functions.


Asunto(s)
Secuencias de Aminoácidos/genética , Axones/fisiología , Dendritas/fisiología , Neuritas/fisiología , Neuronas/fisiología , Tractos Piramidales/citología , Animales , Secuencia de Bases , Ratones , Datos de Secuencia Molecular , Neuronas/citología
5.
BMC Bioinformatics ; 16: 209, 2015 Jul 04.
Artículo en Inglés | MEDLINE | ID: mdl-26141505

RESUMEN

BACKGROUND: The increasing abundance of neuromorphological data provides both the opportunity and the challenge to compare massive numbers of neurons from a wide diversity of sources efficiently and effectively. We implemented a modified global alignment algorithm representing axonal and dendritic bifurcations as strings of characters. Sequence alignment quantifies neuronal similarity by identifying branch-level correspondences between trees. RESULTS: The space generated from pairwise similarities is capable of classifying neuronal arbor types as well as, or better than, traditional topological metrics. Unsupervised cluster analysis produces groups that significantly correspond with known cell classes for axons, dendrites, and pyramidal apical dendrites. Furthermore, the distinguishing consensus topology generated by multiple sequence alignment of a group of neurons reveals their shared branching blueprint. Interestingly, the axons of dendritic-targeting interneurons in the rodent cortex associates with pyramidal axons but apart from the (more topologically symmetric) axons of perisomatic-targeting interneurons. CONCLUSIONS: Global pairwise and multiple sequence alignment of neurite topologies enables detailed comparison of neurites and identification of conserved topological features in alignment-defined clusters. The methods presented also provide a framework for incorporation of additional branch-level morphological features. Moreover, comparison of multiple alignment with motif analysis shows that the two techniques provide complementary information respectively revealing global and local features.


Asunto(s)
Algoritmos , Axones/fisiología , Dendritas/fisiología , Neuronas/citología , Neuronas/fisiología , Animales , Secuencia de Bases , Drosophila/genética , Drosophila/metabolismo , Ratones , Datos de Secuencia Molecular , Filogenia , Homología de Secuencia de Ácido Nucleico
6.
J Neurosci ; 30(5): 1712-20, 2010 Feb 03.
Artículo en Inglés | MEDLINE | ID: mdl-20130180

RESUMEN

Moderate release of the major stress hormones, glucocorticoids (GCs), improves hippocampal function and memory. In contrast, excessive or prolonged elevations produce impairments. Enzymatic degradation and reformation of GCs help to maintain optimal levels within target tissues, including the brain. We hypothesized that expressing a GC-degrading enzyme in hippocampal neurons would attenuate the negative impact of an excessive elevation in GC levels on synaptic physiology and spatial memory. We tested this by expressing 11-beta-hydroxysteroid dehydrogenase (type II) in dentate gyrus granule cells during a 3 d GC treatment followed by examination of synaptic responses in hippocampal slices or spatial performance in the Morris water maze. In adrenalectomized rats with basal GC replacement, additional GC treatments for 3 d reduced synaptic strength and promoted the expression of long-term depression at medial perforant path synapses, increased granule cell and CA1 pyramidal cell excitability, and impaired spatial reference memory (without influencing learning). Expression of 11-beta-hydroxysteroid dehydrogenase (type II), mostly in mature dentate gyrus granule cells, reversed the effects of high GC levels on granule cell and pyramidal cell excitability, perforant path synaptic plasticity, and spatial memory. These data demonstrate the ability of neuroprotective gene expression limited to a specific cell population to both locally and trans-synaptically offset neurophysiological disruptions produced by prolonged increases in circulating stress hormones. This report supplies the first physiological explanation for previously demonstrated cognitive sparing by anti-stress gene therapy approaches and lends additional insight into the hippocampal processes that are important for memory.


Asunto(s)
11-beta-Hidroxiesteroide Deshidrogenasas/metabolismo , Corticosterona/metabolismo , Terapia Genética , Hipocampo/metabolismo , Memoria/fisiología , Plasticidad Neuronal/genética , Células Piramidales/fisiología , Animales , Corticosterona/farmacología , Giro Dentado/enzimología , Expresión Génica , Hipocampo/citología , Masculino , Trastornos de la Memoria/fisiopatología , Ratas , Ratas Sprague-Dawley , Percepción Espacial/fisiología , Sinapsis/fisiología
7.
Front Neuroanat ; 8: 138, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25538569

RESUMEN

Neuronal morphology is diverse among animal species, developmental stages, brain regions, and cell types. The geometry of individual neurons also varies substantially even within the same cell class. Moreover, specific histological, imaging, and reconstruction methodologies can differentially affect morphometric measures. The quantitative characterization of neuronal arbors is necessary for in-depth understanding of the structure-function relationship in nervous systems. The large collection of community-contributed digitally reconstructed neurons available at NeuroMorpho.Org constitutes a "big data" research opportunity for neuroscience discovery beyond the approaches typically pursued in single laboratories. To illustrate these potential and related challenges, we present a database-wide statistical analysis of dendritic arbors enabling the quantification of major morphological similarities and differences across broadly adopted metadata categories. Furthermore, we adopt a complementary unsupervised approach based on clustering and dimensionality reduction to identify the main morphological parameters leading to the most statistically informative structural classification. We find that specific combinations of measures related to branching density, overall size, tortuosity, bifurcation angles, arbor flatness, and topological asymmetry can capture anatomically and functionally relevant features of dendritic trees. The reported results only represent a small fraction of the relationships available for data exploration and hypothesis testing enabled by sharing of digital morphological reconstructions.

8.
Neuroinformatics ; 9(2-3): 233-45, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21519813

RESUMEN

Digital reconstructions of neuronal morphology are used to study neuron function, development, and responses to various conditions. Although many measures exist to analyze differences between neurons, none is particularly suitable to compare the same arborizing structure over time (morphological change) or reconstructed by different people and/or software (morphological error). The metric introduced for the DIADEM (DIgital reconstruction of Axonal and DEndritic Morphology) Challenge quantifies the similarity between two reconstructions of the same neuron by matching the locations of bifurcations and terminations as well as their topology between the two reconstructed arbors. The DIADEM metric was specifically designed to capture the most critical aspects in automating neuronal reconstructions, and can function in feedback loops during algorithm development. During the Challenge, the metric scored the automated reconstructions of best-performing algorithms against manually traced gold standards over a representative data set collection. The metric was compared with direct quality assessments by neuronal reconstruction experts and with clocked human tracing time saved by automation. The results indicate that relevant morphological features were properly quantified in spite of subjectivity in the underlying image data and varying research goals. The DIADEM metric is freely released open source ( http://diademchallenge.org ) as a flexible instrument to measure morphological error or change in high-throughput reconstruction projects.


Asunto(s)
Algoritmos , Procesamiento de Imagen Asistido por Computador/métodos , Procesamiento de Imagen Asistido por Computador/normas , Neuronas/citología , Diseño de Software , Programas Informáticos , Animales , Humanos , Procesamiento de Imagen Asistido por Computador/tendencias , Neuronas/fisiología
9.
Neuroinformatics ; 7(3): 191-4, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19636974

RESUMEN

The constrained tree-edit-distance provides a computationally practical method for comparing morphologies directly without first extracting distributions of other metrics. The application of the constrained tree-edit-distance to hippocampal dendrites by Heumann and Wittum is reviewed and considered in the context of other applications and potential future uses. The method has been used on neuromuscular projection axons for comparisons of topology as well as on trees for comparing plant architectures with particular parameter sets that may inform future efforts in comparing dendritic morphologies. While clearly practical on a small scale, testing and extrapolation of run-times raise questions as to the practicality of the constrained tree-edit-distance for large-scale data mining projects. However, other more efficient algorithms may make use of it as a gold standard for direct morphological comparison.


Asunto(s)
Biología Computacional/métodos , Bases de Datos como Asunto/organización & administración , Citometría de Imagen/métodos , Cómputos Matemáticos , Neuronas/citología , Programas Informáticos , Animales , Simulación por Computador , Dendritas/fisiología , Dendritas/ultraestructura , Humanos , Neuronas/fisiología , Validación de Programas de Computación
10.
Semin Cell Dev Biol ; 19(6): 485-93, 2008 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-18771743

RESUMEN

Neurons vary greatly in size, shape, and complexity depending on their underlying function. Overall size of neuronal trees affects connectivity, area of influence, and other biophysical properties. Relative distributions of neuronal extent, such as the difference between subtrees at branch points, are also critically related to function and activity. This review covers neuromorphological research that analyzes shape and size to elucidate their functional role for different neuron types. We also introduce a novel morphometric, "caulescence", capturing the extent to which trees exhibit a main path. Neuronal tree types differ vastly in caulescence, suggesting potential neurocomputational correlates of this property.


Asunto(s)
Tamaño de la Célula , Neuronas/citología , Animales , Modelos Anatómicos , Morfogénesis
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA