Palaeodiet reconstruction in a woman with probable celiac disease: a stable isotope analysis of bone remains from the archaeological site of Cosa (Italy).

Stable isotope analysis in the reconstruction of human palaeodiets can yield clues to early human subsistence strategies, origins and history of farming and pastoralist societies, and intra- and intergroup social differentiation. In the last 10 years, the method has been extended to the pathological investigation. Stable isotope analysis to better understand a diet-related disease: celiac disease in ancient human bones was carried out. To do this, we analyzed the nitrogen and carbon isotopic composition of human (n = 37) and faunal (n = 8) bone remains from the archaeological site of Cosa at Ansedonia, on the Tyrrhenian coast near Orbetello (Tuscany), including the skeletal remains of a young woman (late 1st century-early 2nd century Common Era [CE]) with morphological and genetic features suggestive of celiac disease. We compared the young woman's isotopic data with those of other individuals recovered at the same site but from two later time periods (6th century CE; 11-12th century CE) and with literature data from other Italian archaeological sites dating to the same period. Her collagen δ(13) C and δ(15) N values differed from those of the samples at the same site, and from most but not all of the contemporary sites. Although the woman's diet appears distinct, chronic malnutrition resulting from severe malabsorption of essential nutrients due to celiac disease may have affected the isotopic composition of her bone collagen.

Nitrogen and carbon stable isotope analysis sheds light on trophic competition between two syntopic land iguana species from Galápagos.

Coexistence between closely related species can lead to intense competition for resources. Stable isotope analysis (SIA) is a reliable tool to estimate the extent of species competition. We employed SIA to evaluate niche partitioning among two syntopic species of Galápagos land iguanas: Conolophus subcristatus and C. marthae. Samples were collected on Wolf Volcano, Isabela Island, where C. marthae is endemic and syntopic with C. subcristatus. We determined δ13C and δ15N ratios and described the isotopic niche of each species using corrected standard ellipse area (SEAc). We tested for differentiation between the isotopic niches, while controlling for sex, body size, spatial location of samples and mean annual primary productivity at capture points, using bivariate linear models. Despite the extensive overlap of the isotopic niches, we found species and sex to be a significant, interacting predictor of a sample's location in the δ13C, δ15N space, indicating the existence of niche partitioning mechanisms acting between species and sexes. We also found that body size and productivity at the capture points, compounded with yet undetermined spatial effects, explain ca. 75% of the differences observed between species and sexes, providing evidence for differential microhabitat and food-items usage. Our study provides essential baselines for evaluating conservation actions for C. marthae, such as the potential translocation to a sanctuary area free of competition from C. subcristatus.

Radon Hazard in Central Italy: Comparison among Areas with Different Geogenic Radon Potential.

Radon (222Rn) is a natural radioactive gas formed in rocks and soil by the decay of its parent nuclide (238-Uranium). The rate at which radon migrates to the surface, be it along faults or directly emanated from shallow soil, represents the Geogenic Radon Potential (GRP) of an area. Considering that the GRP is often linked to indoor radon risk levels, we have conducted multi-disciplinary research to: (i) define local GRPs and investigate their relationship with associated indoor Rn levels; (ii) evaluate inhaled radiation dosages and the associated risk to the inhabitants; and (iii) define radon priority areas (RPAs) as required by the Directive 2013/59/Euratom. In the framework of the EU-funded LIFE-Respire project, a large amount of data (radionuclide content, soil gas samples, terrestrial gamma, indoor radon) was collected from three municipalities located in different volcanic districts of the Lazio region (central Italy) that are characterised by low to high GRP. Results highlight the positive correlation between the radionuclide content of the outcropping rocks, the soil Rn concentrations and the presence of high indoor Rn values in areas with medium to high GRP. Data confirm that the Cimini-Vicani area has inhalation dosages that are higher than the reference value of 10 mSv/y.

Exploring mobility in Italian Neolithic and Copper Age communities.

As a means for investigating human mobility during late the Neolithic to the Copper Age in central and southern Italy, this study presents a novel dataset of enamel oxygen and carbon isotope values (δ18Oca and δ13Cca) from the carbonate fraction of biogenic apatite for one hundred and twenty-six individual teeth coming from two Neolithic and eight Copper Age communities. The measured δ18Oca values suggest a significant role of local sources in the water inputs to the body water, whereas δ13Cca values indicate food resources, principally based on C3 plants. Both δ13Cca and δ18Oca ranges vary substantially when samples are broken down into local populations. Statistically defined thresholds, accounting for intra-site variability, allow the identification of only a few outliers in the eight Copper Age communities, suggesting that sedentary lifestyle rather than extensive mobility characterized the investigated populations. This seems to be also typical of the two studied Neolithic communities. Overall, this research shows that the investigated periods in peninsular Italy differed in mobility pattern from the following Bronze Age communities from more northern areas.

Mapping the geogenic radon potential and radon risk by using Empirical Bayesian Kriging regression: A case study from a volcanic area of central Italy.

A detailed geochemical study on radon related to local geology was carried out in the municipality of Celleno, a little settlement located in the eastern border of the Quaternary Vulsini volcanic district (central Italy). This study included soil-gas and terrestrial gamma dose rate survey, laboratory analyses of natural radionuclides (238U, 226Ra, 232Th, 40K) activity in rocks and soil samples, and indoor radon measurements carried out in selected private and public dwellings. Soil-gas radon and carbon dioxide concentrations range from 6 to 253 kBq/m3 and from 0.3 to11% v/v, respectively. Samples collected from outcropping volcanic and sedimentary rocks highlight: significant concentrations of 238U, 226Ra and 40K for lavas (151, 150 and 1587 Bq/kg, respectively), low concentrations for tuffs (126, 123 and 987 Bq/kg, respectively), and relatively low for sedimentary rocks (108, 109 and 662 Bq/kg, respectively). Terrestrial gamma dose rate values range between 0.130 and 0.417 µSv/h, being in good accordance with the different bedrock types. Indoor radon activity ranges from 162 to 1044 Bq/m3; the calculated values of the annual effective dose varied from 4.08 and 26.31 mSv/y. Empirical Bayesian Kriging Regression (EBKR) was used to develop the Geogenic Radon Potential (GRP) map. EBKR provided accurate predictions of data on a local scale developing a spatial regression model in which soil-gas radon concentrations were considered as the response variable; several proxy variables, derived from geological, topographic and geochemical data, were used as predictors. Risk prediction map for indoor radon was tentatively produced using the Gaussian Geostatistical Simulation and a soil-indoor transfer factor was defined for a 'standard' dwelling (i.e., a dwelling with well-defined construction properties). This approach could be successfully used in the case of homogeneous building characteristics and territory with uniform geological characteristics.