1.
Bioconjug Chem
; 30(10): 2539-2543, 2019 10 16.
Artículo
en Inglés
| MEDLINE
| ID: mdl-31560523
RESUMEN
The functionalization of proteins with different cargo molecules is highly desirable for a broad range of applications. However, the reproducible production of defined conjugates with multiple functionalities is a significant challenge. Herein, we report the dual site-specific labeling of an antibody fragment, utilizing the orthogonal Sortase A and π-clamp conjugation methods, and demonstrate that binding of the antibody fragment to its target receptor is retained after dual labeling.