Parent-adolescent reminiscing and youth psychopathology: A cross-sectional and longitudinal investigation.

INTRODUCTION: Robust research links qualities of parent-child discussions about past emotion-laden events to socioemotional development and broader psychological outcomes during childhood. The role of parent-adolescent reminiscing in adolescent psychological adjustment, however, has received less attention, despite adolescence being a time of heightened vulnerability for the development of internalizing symptoms. In the current multimethod study, we investigated cross-sectional and longitudinal associations between the qualities of conversations between mothers and adolescents (ages 13-16) and adolescents' internalizing problems. METHODS: Participants were 67 mother-adolescent dyads (total N = 134, 58.8% of youth identified as female) located across regions of New Zealand/Aotearoa. Each dyad discussed a past shared conflict, coded for supportive and unsupportive reminiscing conversational qualities with an adapted dyadic coding scheme. Youth internalizing symptoms were assessed at two-time points, 12-month apart. RESULTS: Dyadic structural equation modeling analyzed cross-sectional and longitudinal relationships between conversational qualities and adolescents' internalizing problems. Findings indicated concurrent associations between unsupportive mother-adolescent reminiscing qualities and heightened youth anxiety symptoms: Specifically, mothers' avoidance and lower levels of emotion discussion and adolescents' emotional disengagement were linked to greater youth anxiety symptoms. Moreover, increases in youth anxiety symptoms 12 months later were weaker for youth who engaged in greater levels of the supportive reminiscing qualities of balanced emotion discussion and active problem-solving. CONCLUSIONS: These novel findings highlight the transactional nature and complex dynamics of reminiscing during adolescence and their relationship with youth mental health, which has implications for theory and clinical practice.

AiDAPT: automated insulin delivery amongst pregnant women with type 1 diabetes: a multicentre randomized controlled trial - study protocol.

BACKGROUND: Pregnant women with type 1 diabetes strive for tight glucose targets (3.5-7.8 mmol/L) to minimise the risks of obstetric and neonatal complications. Despite using diabetes technologies including continuous glucose monitoring (CGM), insulin pumps and contemporary insulin analogues, most women struggle to achieve and maintain the recommended pregnancy glucose targets. This study aims to evaluate whether the use of automated closed-loop insulin delivery improves antenatal glucose levels in pregnant women with type 1 diabetes. METHODS/DESIGN: A multicentre, open label, randomized, controlled trial of pregnant women with type 1 diabetes and a HbA1c of ≥48 mmol/mol (6.5%) at pregnancy confirmation and ≤ 86 mmol/mol (10%) at randomization. Participants who provide written informed consent before 13 weeks 6 days gestation will be entered into a run-in phase to collect 96 h (24 h overnight) of CGM glucose values. Eligible participants will be randomized on a 1:1 basis to CGM (Dexcom G6) with usual insulin delivery (control) or closed-loop (intervention). The closed-loop system includes a model predictive control algorithm (CamAPS FX application), hosted on an android smartphone that communicates wirelessly with the insulin pump (Dana Diabecare RS) and CGM transmitter. Research visits and device training will be provided virtually or face-to-face in conjunction with 4-weekly antenatal clinic visits where possible. Randomization will stratify for clinic site. One hundred twenty-four participants will be recruited. This takes into account 10% attrition and 10% who experience miscarriage or pregnancy loss. Analyses will be performed according to intention to treat. The primary analysis will evaluate the change in the time spent in the target glucose range (3.5-7.8 mmol/l) between the intervention and control group from 16 weeks gestation until delivery. Secondary outcomes include overnight time in target, time above target (> 7.8 mmol/l), standard CGM metrics, HbA1c and psychosocial functioning and health economic measures. Safety outcomes include the number and severity of ketoacidosis, severe hypoglycaemia and adverse device events. DISCUSSION: This will be the largest randomized controlled trial to evaluate the impact of closed-loop insulin delivery during type 1 diabetes pregnancy. TRIAL REGISTRATION: ISRCTN 56898625 Registration Date: 10 April, 2018.

Investigating whether controlling and aggressive relationship behaviors are discriminant.

Control is theorized as central to intimate partner aggression (IPA). Tools measuring nonphysical "controlling behaviors" in relationships have therefore been developed to identify the latent construct of control. However, the underlying assumption that "controlling behaviors" form a distinct subset of IPA has not been validated. This study investigates the divergent validity of acts considered as "controlling behaviors" against other aggressive acts used in relationships. The IPA and relationship literatures were reviewed to identify 1,397 items involving "controlling," physical, sexual, and psychologically aggressive acts perpetrated and/or experienced by an intimate partner. In total, 101 item pairs were identified and used to measure IPA tactics across these categories. In Study 1, exploratory factor analysis in a community sample (N = 561) found no evidence of a distinct factor of "controlling behaviors." Behaviors labeled as "controlling" in existing measures were distributed across other factors, including "eclectic aggression," "direct psychological aggression," and "monitoring acts." In Study 2A (N = 424 students), confirmatory factor analysis replicated the results of Study 1 and established configural measurement invariance (Study 2B), indicating no evidence for psychometric differences between samples. These results indicate that behaviors described as "controlling" in existing measures were not statistically distinguishable from other forms of IPA, and suggest that future research should investigate motivational, rather than behavioral, differences in the use of IPA. The findings challenge research to confirm whether a set of discrete behaviors can be used to accurately identify control in relationships and question the validity of tools that adopt this methodology.

Study protocol: ASCRIBED: the impact of Acute SystematiC inflammation upon cerebRospinal fluId and blood BiomarkErs of brain inflammation and injury in dementia: a study in acute hip fracture patients.

BACKGROUND: Hip fracture represents a substantial acute inflammatory trauma, which may constitute a significant insult to the degenerating brain. Research suggests that an injury of this kind can affect memory and thinking in the future but it is unclear whether, and how, inflammatory trauma injures the brain. The impact of Acute SystematiC inflammation upon cerebRospinal fluId and blood BiomarkErs of brain inflammation and injury in Dementia: a study in acute hip fracture patients (ASCRIBED) explores this relationship, to understand the effect of inflammation on the progression of dementia. METHODS: This protocol describes a multi-centre sample collection observational study. The study utilises the unique opportunity provided by hip fracture operations undertaken via spinal anaesthesia to collect cerebrospinal fluid (CSF) and blood, to investigate the impact of acute brain inflammation caused by hip fracture on the exacerbation of dementia. We will recruit 200 hip fracture patients with a diagnosis or evidence of dementia; and 200 hip fracture patients without dementia. We will also recruit 'Suitable informants', individuals in regular contact with the patient, to provide further proxy evidence of a patient's potential cognitive decline. We will compare these 400 samples with existing CSF and blood samples from a cohort of dementia patients who had not experienced a systemic inflammatory response due to injury. This will provide a comparison between patients with and without dementia who are suffering a systemic inflammatory response; with stable patients living with dementia. DISCUSSION: We will test the hypothesis that hip fracture patients living with dementia show elevated markers of brain inflammation, as well as neuronal injury and Alzheimer-related plaque pathology, in comparison to (1) stable patients living with dementia and (2) hip fracture patients without dementia, as measured by biomarkers in CSF and blood. The findings will address the hypothesis that systemic inflammatory events can exacerbate underlying dementia and inform the search for new treatments targeting inflammation in dementia. TRIAL REGISTRATION: ISRCTN43803769 . Registered 11 May 2017.

Age- and location-related changes in microglial function.

Inflammation in the central nervous system (CNS) is primarily regulated by microglia. No longer considered a homogenous population, microglia display a high degree of heterogeneity, immunological diversity and regional variability in function. Given their low rate of self-renewal, the microenvironment in which microglia reside may play an important role in microglial senescence. This study examines age-related changes in microglia in the brain and spinal cord. Using ex-vivo flow cytometry analyses, functional assays were performed to assess changes in microglial morphology, oxidative stress, cytokine production, and phagocytic activity with age in both the brain and spinal cord. The regional CNS environment had a significant effect on microglial activity with age. Blood-CNS barrier permeability was greater in the aging spinal cord compared with aging brain; this was associated with increased tissue cytokine levels. Aged microglia had deficits in phagocytosis at baseline and after stimulus-induced activation. The identification of age-specific, high scatter microglia together with the use of ex-vivo functional analyses provides the first functional characterization of senescent microglia. Age and regional-specificity of CNS disease should be taken into consideration when developing immune-modulatory treatments.

V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in western blotting.

The western blot is a very useful and widely adopted lab technique, but its execution is challenging. The workflow is often characterized as a "black box" because an experimentalist does not know if it has been performed successfully until the last of several steps. Moreover, the quality of western blot data is sometimes challenged due to a lack of effective quality control tools in place throughout the western blotting process. Here we describe the V3 western workflow, which applies stain-free technology to address the major concerns associated with the traditional western blot protocol. This workflow allows researchers: 1) to run a gel in about 20-30 min; 2) to visualize sample separation quality within 5 min after the gel run; 3) to transfer proteins in 3-10 min; 4) to verify transfer efficiency quantitatively; and most importantly 5) to validate changes in the level of the protein of interest using total protein loading control. This novel approach eliminates the need of stripping and reprobing the blot for housekeeping proteins such as ß-actin, ß-tubulin, GAPDH, etc. The V3 stain-free workflow makes the western blot process faster, transparent, more quantitative and reliable.

A defined methodology for reliable quantification of Western blot data.

Chemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative method for measuring the relative expression of the target proteins is still debatable. This is mainly due to the various steps, techniques, reagents, and detection methods that are used to obtain the associated data. In order to have confidence in densitometric data from western blots, researchers should be able to demonstrate statistically significant fold differences in protein expression. This entails a necessary evolution of the procedures, controls, and the analysis methods. We describe a methodology to obtain reliable quantitative data from chemiluminescent western blots using standardization procedures coupled with the updated reagents and detection methods.