Asunto(s)
Bancos de Muestras Biológicas , Deleción Cromosómica , Mapeo Cromosómico/métodos , Embrión de Mamíferos/citología , Células Madre/citología , Animales , Quimera/genética , Células Clonales , Marcadores Genéticos , Ratones , Ratones Endogámicos C57BL , Polimorfismo Conformacional Retorcido-Simple , Mapeo de Híbrido por Radiación , Eliminación de Secuencia/genéticaRESUMEN
Genetic strategies for the post-genomic sequence age will be designed to provide information about gene function in a myriad of physiological processes. Here an ENU mutagenesis program (http://reprogenomics.jax.org) is described that is generating a large resource of mutant mouse models of infertility; male and female mutants with defects in a wide range of reproductive processes are being recovered. Identification of the genes responsible for these defects, and the pathways in which these genes function, will advance the fields of reproduction research and medicine. Importantly, this program has potential to reveal novel human contraceptive targets.
Asunto(s)
Anticonceptivos , Ratones Mutantes/genética , Modelos Genéticos , Reproducción/genética , Animales , Cruzamientos Genéticos , Evaluación Preclínica de Medicamentos , Femenino , Humanos , Infertilidad/genética , Masculino , Ratones , Ratones Mutantes/fisiología , Mutagénesis , Fenotipo , Reproducción/fisiologíaRESUMEN
Oviductal damage from infectious salpingitis is a principle cause of ectopic pregnancy. To examine the role of Chlamydia trachomatis in patients with ectopic pregnancy, 24 patients undergoing laparotomy for ectopic pregnancy were evaluated by assessment of chlamydial serology and chlamydial cultures of tubal biopsy specimens. The patients were divided into two groups based on the presence or absence of gross abnormalities in the fallopian tube contralateral to the ectopic gestation. Ten patients in group I showed gross evidence of chronic salpingitis in the contralateral tube; 14 patients in group II had normal-appearing contralateral tubes. Chlamydial cultures were negative in both groups. Chlamydial immunoglobulin M antibodies assayed by indirect microimmunofluorescence were negative in both groups (less than or equal to 1:32). Mean geometric immunoglobulin G titers for C. trachomatis were significantly higher in the patients with evidence of contralateral chronic salpingitis (78.9 versus 13.1). These findings suggest that C. trachomatis may be a major cause of oviductal damage, which predisposes to ectopic pregnancy.
Asunto(s)
Infecciones por Chlamydia/complicaciones , Embarazo Tubario/etiología , Salpingitis/etiología , Adulto , Infecciones por Chlamydia/diagnóstico , Chlamydia trachomatis , Femenino , Humanos , Embarazo , Pruebas SerológicasRESUMEN
The wild-type New Jersey serotype of vesicular stomatitis virus generated two types of defective interfering T-particles. The physical properties of these particles and the wild-type virion were determined by laser light scattering spectroscopy, sedimentation measurements, and electron microscopy.