Plant-Based Decellularization: A Novel Approach for Perfusion-Compatible Tissue Engineering Structures.

This study explores the potential of plant-based decellularization in regenerative medicine, a pivotal development in tissue engineering focusing on scaffold development, modification, and vascularization. Plant decellularization involves removing cellular components from plant structures, offering an eco-friendly and cost-effective alternative to traditional scaffold materials. The use of plant-derived polymers is critical, presenting both benefits and challenges, notably in mechanical properties. Integration of plant vascular networks represents a significant bioengineering breakthrough, aligning with natural design principles. The paper provides an in-depth analysis of development protocols, scaffold fabrication considerations, and illustrative case studies showcasing plant-based decellularization applications. This technique is transformative, offering sustainable scaffold design solutions with readily available plant materials capable of forming perfusable structures. Ongoing research aims to refine protocols, assess long-term implications, and adapt the process for clinical use, indicating a path toward widespread adoption. Plant-based decellularization holds promise for regenerative medicine, bridging biological sciences with engineering through eco-friendly approaches. Future perspectives include protocol optimization, understanding long-term impacts, clinical scalability, addressing mechanical limitations, fostering collaboration, exploring new research areas, and enhancing education. Collectively, these efforts envision a regenerative future where nature and scientific innovation converge to create sustainable solutions, offering hope for generations to come.

Effect of different types and shapes of microplastics on the growth of lettuce.

The presence of microplastics in agricultural soils has emerged as a significant environmental concern due to their persistent nature. Microplastics of different properties (i.e., types, shapes, size, concentration) are present in the environment, but the studies on the effect of microplastics having different properties are limited. Thus, this study investigated the effects of different microplastics (low-density polyethylene (LDPE) fragments, polyvinyl chloride (PVC) fragments, and LDPE fiber) in soil on the growth of lettuce (Lactuca sativa L.). Pot tests were carried out to study the effect of a range of microplastic concentrations and different shapes and types of microplastics in soil on the lettuce growth. The different growth parameters such as lettuce weight, lengths, and chlorophyll contents were measured and compared. The results showed that the adverse effects of the microplastics on the lettuce growth increased with increasing microplastic concentration. The effects of LDPE fragments and fibers on the root weights and the chlorophyll contents were microplastic shape-dependent. Also, the effects of LDPE fragments and PVC fragments on the shoot and root weights and the chlorophyll contents were microplastic type-dependent. Among the three microplastics studied, LDPE fragments tend to have greater effects on the lettuce growth than the other microplastics. Overall, the results show that the effects of microplastics on different growth parameters of lettuce can be shape- and/or type-dependent. The presence of microplastics having different properties make the understanding the effects of microplastics on plants difficult, and this necessitates further studies.