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1.
Proc Natl Acad Sci U S A ; 110(51): 20521-6, 2013 Dec 17.
Artículo en Inglés | MEDLINE | ID: mdl-24306931

RESUMEN

The intracellular bacterial pathogen Listeria monocytogenes is capable of remodelling the actin cytoskeleton of its host cells such that "comet tails" are assembled powering its movement within cells and enabling cell-to-cell spread. We used cryo-electron tomography to visualize the 3D structure of the comet tails in situ at the level of individual filaments. We have performed a quantitative analysis of their supramolecular architecture revealing the existence of bundles of nearly parallel hexagonally packed filaments with spacings of 12-13 nm. Similar configurations were observed in stress fibers and filopodia, suggesting that nanoscopic bundles are a generic feature of actin filament assemblies involved in motility; presumably, they provide the necessary stiffness. We propose a mechanism for the initiation of comet tail assembly and two scenarios that occur either independently or in concert for the ensuing actin-based motility, both emphasizing the role of filament bundling.


Asunto(s)
Listeria monocytogenes/ultraestructura , Listeriosis , Modelos Moleculares , Fibras de Estrés/ultraestructura , Línea Celular , Microscopía por Crioelectrón/métodos , Humanos , Listeria monocytogenes/metabolismo , Fibras de Estrés/metabolismo
2.
J Struct Biol ; 177(1): 135-44, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21907807

RESUMEN

Cryo-electron tomography allows to visualize individual actin filaments and to describe the three-dimensional organization of actin networks in the context of unperturbed cellular environments. For a quantitative characterization of actin filament networks, the tomograms must be segmented in a reproducible manner. Here, we describe an automated procedure for the segmentation of actin filaments, which combines template matching with a new tracing algorithm. The result is a set of lines, each one representing the central line of a filament. As demonstrated with cryo-tomograms of cellular actin networks, these line sets can be used to characterize filament networks in terms of filament length, orientation, density, stiffness (persistence length), or the occurrence of branching points.


Asunto(s)
Citoesqueleto de Actina/ultraestructura , Tomografía con Microscopio Electrónico/métodos , Algoritmos , Animales , Línea Celular , Microscopía por Crioelectrón/métodos , Dictyostelium/crecimiento & desarrollo , Dictyostelium/aislamiento & purificación , Electrones , Procesamiento de Imagen Asistido por Computador , Ratas
3.
J Struct Biol ; 173(1): 77-85, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-20719249

RESUMEN

Classification and averaging of sub-tomograms can improve the fidelity and resolution of structures obtained by electron tomography. Here we present a three-dimensional (3D) maximum likelihood algorithm--MLTOMO--which is characterized by integrating 3D alignment and classification into a single, unified processing step. The novelty of our approach lies in the way we calculate the probability of observing an individual sub-tomogram for a given reference structure. We assume that the reference structure is affected by a 'compound wedge', resulting from the summation of many individual missing wedges in distinct orientations. The distance metric underlying our probability calculations effectively down-weights Fourier components that are observed less frequently. Simulations demonstrate that MLTOMO clearly outperforms the 'constrained correlation' approach and has advantages over existing approaches in cases where the sub-tomograms adopt preferred orientations. Application of our approach to cryo-electron tomographic data of ice-embedded thermosomes revealed distinct conformations that are in good agreement with results obtained by previous single particle studies.


Asunto(s)
Algoritmos , Interpretación Estadística de Datos , Tomografía con Microscopio Electrónico/métodos , Tomografía con Microscopio Electrónico/estadística & datos numéricos , Modelos Moleculares , Termosomas/química , Tomografía con Microscopio Electrónico/clasificación , Funciones de Verosimilitud
4.
Structure ; 20(4): 593-603, 2012 Apr 04.
Artículo en Inglés | MEDLINE | ID: mdl-22483107

RESUMEN

Tripeptidyl-peptidase II (TPPII) is a high molecular mass (∼5 MDa) serine protease, which is thought to act downstream of the 26S proteasome, cleaving peptides released by the latter. Here, the structure of human TPPII (HsTPPII) has been determined to subnanometer resolution by cryoelectron microscopy and single-particle analysis. The complex is built from two strands forming a quasihelical structure harboring a complex system of inner cavities. HsTPPII particles exhibit some polymorphism resulting in complexes consisting of nine or of eight dimers per strand. To obtain deeper insights into the architecture and function of HsTPPII, we have created a pseudoatomic structure of the HsTPPII spindle using a comparative model of HsTPPII dimers and molecular dynamics flexible fitting. Analyses of the resulting hybrid structure of the HsTPPII holocomplex provide new insights into the mechanism of maturation and activation.


Asunto(s)
Aminopeptidasas/química , Dipeptidil-Peptidasas y Tripeptidil-Peptidasas/química , Simulación de Dinámica Molecular , Serina Endopeptidasas/química , Aminopeptidasas/genética , Aminopeptidasas/metabolismo , Microscopía por Crioelectrón , Dipeptidil-Peptidasas y Tripeptidil-Peptidasas/genética , Dipeptidil-Peptidasas y Tripeptidil-Peptidasas/metabolismo , Activación Enzimática , Escherichia coli , Holoenzimas/química , Holoenzimas/genética , Holoenzimas/metabolismo , Humanos , Peso Molecular , Multimerización de Proteína , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Serina Endopeptidasas/genética , Serina Endopeptidasas/metabolismo
5.
HFSP J ; 3(6): 412-27, 2009 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20514132

RESUMEN

Propagating actin waves are dynamic supramolecular structures formed by the self-assembly of proteins within living cells. They are built from actin filaments together with single-headed myosin, the Arp23 complex, and coronin in a defined three-dimensional order. The function of these waves in structuring the cell cortex is studied on the substrate-attached surface of Dictyostelium cells by the use of total internal reflection fluorescence (TIRF) microscopy. Actin waves separate two areas of the cell cortex from each other, which are distinguished by the arrangement of actin filaments. The Arp23 complex dominates in the area enclosed by a wave, where it has the capacity of building dendritic structures, while the proteins prevailing in the external area, cortexillin I and myosin-II, bundle actin filaments and arrange them in antiparallel direction. Wave propagation is accompanied by transitions in the state of actin with a preferential period of 5 min. Wave generation is preceded by local fluctuations in actin assembly, some of the nuclei of polymerized actin emanating from clathrin-coated structures, others emerging independently. The dynamics of phase transitions has been analyzed to provide a basis for modeling the nonlinear interactions that produce spatio-temporal patterns in the actin system of living cells.

6.
Biochem Biophys Res Commun ; 353(1): 115-20, 2007 Feb 02.
Artículo en Inglés | MEDLINE | ID: mdl-17173858

RESUMEN

The 26S proteasome is the key enzyme of intracellular protein degradation in eukaryotic cells. It is a multisubunit complex of 2.5 MDa confining the proteolytic action to an inner compartment with tightly controlled access. Structural studies of this intriguing molecular machine have been hampered by its intrinsic instability and its dynamics. Here we have used an unconventional approach to obtain a three-dimensional structure of the holocomplex uncompromised by preparation-induced alterations and unbiased by any starting model. We have performed a tomographic reconstruction, followed by averaging over approx. 150 individual reconstructions, of Drosophila 26S proteasomes suspended in a thin layer of amorphous ice.


Asunto(s)
Microscopía por Crioelectrón/métodos , Proteínas de Drosophila/ultraestructura , Complejo de la Endopetidasa Proteasomal/ultraestructura , Tomografía/métodos , Cristalografía/métodos , Conformación Proteica
7.
J Struct Biol ; 150(1): 1-10, 2005 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-15797725

RESUMEN

Oat beta-glucosidase in plastid hydrolyzes avenacosides to C26-desgluco-avenacosides to combat against fungal infections. The enzyme has a unique quaternary protein structure of a three-dimensionally radiated assembly of long fibrillae. We elucidated the fibrillar assembly of oat type 1 beta-glucosidase by means of cryo-electron microscopy, enzyme kinetics and chemical modification. It was assembled by linear stacking of hollow trimeric units and the resulting fibril had a long central tunnel connecting to the outer medium via regularly distributed side fenestrations. The enzyme active sites were located within the central tunnel. This unique multimer assembly increased enzyme affinity to avenacosides, in vivo substrates, and may function to discriminate avenacosides from many other kinds of beta-glucoside in oat. The fibrillar multimer of oat beta-glucosidase is a novel quaternary protein structure for enzyme supramolecular assembly that may have a functional role in the regulation of enzyme affinity.


Asunto(s)
Avena/enzimología , beta-Glucosidasa/química , Sitios de Unión , Microscopía por Crioelectrón , Conformación Proteica , Especificidad por Sustrato , beta-Glucosidasa/aislamiento & purificación
8.
Proc Natl Acad Sci U S A ; 102(29): 10135-40, 2005 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-16006508

RESUMEN

In eukaryotes, tripeptidyl peptidase II (TPPII) is a crucial component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It is an amino peptidase belonging to the subtilase family removing tripeptides from the free N terminus of oligopeptides. The 150-kDa subunits of Drosophila TPPII assemble into a giant proteolytic complex of 6 MDa with a remarkable architecture consisting of two segmented and twisted strands that form a spindle-shaped structure. A refined 3D model has been obtained by cryoelectron microscopy, which reveals details of the molecular architecture and, in conjunction with biochemical data, provides insight into the assembly mechanism. The building blocks of this complex are apparently dimers, within which the 150-kDa monomers are oriented head to head. Stacking of these dimers leads to the formation of twisted single strands, two of which comprise the fully assembled spindle. This spindle also forms when TPPII is heterologously expressed in Escherichia coli, demonstrating that no scaffolding protein is required for complex formation and length determination. Reciprocal interactions of the N-terminal part of subunits from neighboring strands are probably involved in the formation of the native quaternary structure, lending the TPPII spindle a stability higher than that of single strands.


Asunto(s)
Drosophila/enzimología , Modelos Moleculares , Serina Endopeptidasas/química , Serina Endopeptidasas/metabolismo , Aminopeptidasas , Animales , Microscopía por Crioelectrón , Dipeptidil-Peptidasas y Tripeptidil-Peptidasas , Endopeptidasa K , Escherichia coli , Imagenología Tridimensional , Microscopía Electrónica , Serina Endopeptidasas/ultraestructura
9.
J Struct Biol ; 149(3): 227-34, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15721576

RESUMEN

Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.


Asunto(s)
Procesamiento de Imagen Asistido por Computador , Programas Informáticos , Tomografía/métodos , Algoritmos , Humanos , Tomografía/instrumentación , Interfaz Usuario-Computador
10.
J Struct Biol ; 138(1-2): 105-13, 2002.
Artículo en Inglés | MEDLINE | ID: mdl-12160706

RESUMEN

An automatic image segmentation method is used to improve processing and visualization of data obtained by electron microscopy. Exploiting affinity criteria between pixels, e.g., proximity and gray level similarity, in conjunction with an eigenvector analysis, the image is subdivided into areas which correspond to objects or meaningful regions. Extending a proposal by Shi and Malik (1997, Proceedings of the IEEE conference on Computer Vision and Pattern Recognition, pp. 731-737) the approach was adapted to the field of electron microscopy, especially to three-dimensional application as needed by electron tomography. Theory, implementation, parameter setting, and results obtained with a variety of data are presented and discussed. The method turns out to be a powerful tool for visualization with the potential for further improvement by developing and tuning new affinity.


Asunto(s)
Imagenología Tridimensional/métodos , Microscopía Electrónica/métodos , Modelos Teóricos , Algoritmos , Desulfurococcaceae/citología , Desulfurococcaceae/ultraestructura , Procesamiento de Imagen Asistido por Computador/métodos , Siphoviridae/ultraestructura , Tomografía Computarizada por Rayos X
11.
J Struct Biol ; 142(2): 301-10, 2003 May.
Artículo en Inglés | MEDLINE | ID: mdl-12713958

RESUMEN

Reference bias refers to a common problem in fitting experimental data to an initial model. Given enough free parameters, a good fit of any experimental data to the model can be obtained, even if the experimental data contain only noise. Reference-based alignment methods used in electron microscopy (EM) are subject to this type of bias, in that images containing pure noise can regenerate the reference. Cross-validation is based on the idea that the experimental data used to assess the validity of the fitting should not be the same data as were used to do the fitting. Here we present the application of cross-validation to one form of reference-based alignment: 3D-projection matching in single-particle reconstructions. Our results show that reference bias is indeed present in reconstructions, but that the effect is small for real data compared to that for random noise, and that this difference in behavior is magnified, rather than diminished, during iterative refinement.


Asunto(s)
Sustancias Macromoleculares , Microscopía Electrónica/métodos , Modelos Teóricos , Escherichia coli/química , Interpretación de Imagen Asistida por Computador , Ribosomas/química
12.
J Struct Biol ; 141(1): 34-42, 2003 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-12576018

RESUMEN

The hyperthermophilic archaeon Pyrodictium grows in the form of a macroscopically visible network. It consists of cells entrapped in an extracellular matrix of hollow tubules, the "cannulae." Here, we present the three-dimensional structure of a single cell in conjunction with two extracellular cannulae, as determined by cryo-electron microscopy. To achieve this, the information from two independent tilt series of the same specimen was combined, with the specimen rotated in the second series. In the three-dimensional tomographic reconstruction, we were able to trace the two cannulae in their full length, in particular, also inside the cell. One cannula enters the periplasmic space, while the other cannula contacts the surface of the cell, the S-layer. This indicates that the cannulae interconnect individual cells with each other on the level of their periplasmic space; we do not, however, have evidence that they enter the cytoplasm of the cells. The implications of these data for possible functions of the cannulae are discussed.


Asunto(s)
Citoplasma/metabolismo , Desulfurococcaceae/metabolismo , Matriz Extracelular/metabolismo , Tomografía/métodos , Microscopía por Crioelectrón , Electrones , Procesamiento de Imagen Asistido por Computador , Microscopía Electrónica , Orgánulos/ultraestructura , Periplasma/metabolismo
13.
J Struct Biol ; 138(1-2): 74-84, 2002.
Artículo en Inglés | MEDLINE | ID: mdl-12160703

RESUMEN

Large nuclear ribonucleoprotein particles, which can be viewed as the naturally assembled precursor messenger RNA (pre-mRNA) processing machine, were analyzed in frozen-hydrated preparations by cryoelectron microscopy. A general and reproducible strategy for preparing ice-embedded large nuclear ribonucleoprotein (lnRNP) particles at sufficiently high concentration was developed. Taking advantage of their negatively charged components, the lnRNP particles are adsorbed and thus concentrated on a positively charged lipid monolayer while preserving their native structure. Using this approach we carried out cryoelectron tomography and three-dimensional image reconstruction of individual lnRNP particles. The study revealed a structure similar to that of negatively stained particles studied previously, yet with additional features. The small additional domain visualized in negative stain appeared to be larger in the ice preparations. In addition, using image restoration from focus series of ice-embedded lnRNP particles, new features such as holes within the subunits were visualized in two dimensions, and it was shown that the subunits are interconnected via a fiber, very likely formed by the pre-mRNA. This finding supports the model that each subunit represents a spliceosome that splices out the intron wound around it.


Asunto(s)
Estructuras del Núcleo Celular/ultraestructura , Microscopía por Crioelectrón , Ribonucleoproteínas/ultraestructura , Tomografía Computarizada por Rayos X , Células HeLa , Humanos , Procesamiento de Imagen Asistido por Computador , Imagenología Tridimensional/métodos , Lípidos/química , Precursores del ARN , Procesamiento Postranscripcional del ARN , Ribonucleoproteínas/química , Empalmosomas
14.
Proc Natl Acad Sci U S A ; 99(22): 14153-8, 2002 Oct 29.
Artículo en Inglés | MEDLINE | ID: mdl-12391313

RESUMEN

Electron tomograms of intact frozen-hydrated cells are essentially three-dimensional images of the entire proteome of the cell, and they depict the whole network of macromolecular interactions. However, this information is not easily accessible because of the poor signal-to-noise ratio of the tomograms and the crowded nature of the cytoplasm. Here, we describe a template matching algorithm that is capable of detecting and identifying macromolecules in tomographic volumes in a fully automated manner. The algorithm is based on nonlinear cross correlation and incorporates elements of multivariate statistical analysis. Phantom cells, i.e., lipid vesicles filled with macromolecules, provide a realistic experimental scenario for an assessment of the fidelity of this approach. At the current resolution of approximately 4 nm, macromolecules in the size range of 0.5-1 MDa can be identified with good fidelity.


Asunto(s)
Algoritmos , Proteínas Arqueales/análisis , Chaperoninas/análisis , Cisteína Endopeptidasas/análisis , Complejos Multienzimáticos/análisis , Vesículas Cubiertas , Microscopía por Crioelectrón/métodos , Liposomas/química , Análisis Multivariante , Dinámicas no Lineales , Complejo de la Endopetidasa Proteasomal
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