Long-term suboptimal dietary trace element supply does not affect trace element homeostasis in murine cerebellum.

The ageing process is associated with alterations of systemic trace element (TE) homeostasis increasing the risk, e.g. neurodegenerative diseases. Here, the impact of long-term modulation of dietary intake of copper, iron, selenium, and zinc was investigated in murine cerebellum. Four- and 40-wk-old mice of both sexes were supplied with different amounts of those TEs for 26 wk. In an adequate supply group, TE concentrations were in accordance with recommendations for laboratory mice while suboptimally supplied animals received only limited amounts of copper, iron, selenium, and zinc. An additional age-adjusted group was fed selenium and zinc in amounts exceeding recommendations. Cerebellar TE concentrations were measured by inductively coupled plasma-tandem mass spectrometry. Furthermore, the expression of genes involved in TE transport, DNA damage response, and DNA repair as well as selected markers of genomic stability [8-oxoguanine, incision efficiency toward 8-oxoguanine, 5-hydroxyuracil, and apurinic/apyrimidinic sites and global DNA (hydroxy)methylation] were analysed. Ageing resulted in a mild increase of iron and copper concentrations in the cerebellum, which was most pronounced in the suboptimally supplied groups. Thus, TE changes in the cerebellum were predominantly driven by age and less by nutritional intervention. Interestingly, deviation from adequate TE supply resulted in higher manganese concentrations of female mice even though the manganese supply itself was not modulated. Parameters of genomic stability were neither affected by age, sex, nor diet. Overall, this study revealed that suboptimal dietary TE supply does not substantially affect TE homeostasis in the murine cerebellum.

Experiencia de padres/madres al cuidado de niños de 5 a 10 años con trastorno del espectro autista / Parent's experience caring for children 5 to 10 years old with autism spectrum disorder / Experiência dos pais a cuidar de crianças dos 5 aos10 anos com desordem do espectro do autismo

INTRODUCCIÓN: El trastorno del espectro autista contempla una serie de trastornos evolutivos comprometiendo diversos ejes. OBJETIVO: Develar experiencias vividas por los padres/madres al cuidado de niños/as de 5-10 años con Trastorno de espectro autista (TEA). METODOLOGÍA: Estudio cualitativo paradigma interpretativo diseño fenomenológico, en el que se recogieron las vivencias a través de entrevistas en profundidad a madres/padres que participan en las fundaciones de Chile, las que fueron grabadas y transcritas textualmente por las investigadoras con previo consentimiento de los participantes. Para el análisis de los discursos se cumplió con el rigor metodológico. RESULTADOS: Tras analizar las entrevistas, se develó un proceso dinámico y continuo de múltiples vivencias que permitieron a los participantes pasar desde la experiencia al cuidado de niños con TEA de alto funcionamiento, hasta las mejoras que se podrían aplicar en salud. CONCLUSIÓN: El hecho de tener un hijo/a con TEA lo convierte en un proceso complejo, el cual genera diversas emociones en los padres/madres, pero que a pesar de ello les ha permitido vivir un proceso de adaptación, mayor conocimiento teórico y práctico de la condición, permitiendo un crecimiento y desarrollo favorable para el niño/niña y las familias.

INTRODUCTION: Autism spectrum disorder contemplates a series of developmental disorderscompromising several axes. OBJECTIVE: To unveil experiences lived by parents caring forchildren aged 5-10 years with a high-functioning Autism Spectrum Disorder. METHODOLOGY: Qualitative study, interpretative paradigm,phenomenological design,the experiences collected through in-depth interviews with parents participating in foundations in Chile, which were recorded and transcribed verbatim by there searchers with the prior consent of the participants.For the analysis of the speeches, the methodological rigor meets. RESULTS: After analyzing the nterviews, a dynamic and continuous process of multiple experiences was revealed, which allowed the participants to go from the experience of caring for children with a high-functioning Autism Spectrum Disorderto the applicable improvements in health.CONCLUSION:The fact of having a child with ASD makes it a complex process, which generates diverseemotions in the parents, but despite this, it has allowed them to live a process of adaptation,greater theoretical and practical knowledge of the condition, allowing a favorable growth anddevelopmentforthechild and the families.

INTRODUÇÃO: A perturbação do espectro autista contempla uma série de perturbações dedesenvolvimento que comprometem vários eixos. OBJETIVO: Revelar experiências vividas por pais que cuidam decrianças dos5-10 anos comTranstorno do Espectro Autista(TEA). METODOLOGIA: Estudo qualitativo fenomenológico, no qualforamrecolhidas experiênciasatravés de entrevistas aprofundadas com mães e pais que participam em fundações no Chile, as quais foram gravadas e transcritas textualmente como consentimento prévio dos participantes.Para a análise dos dados,foi respeitado o rigor metodológico.RESULTADOS: Após análised as entrevistas,foi revelado um processo dinâmico e contínuo de múltiplas experiências,o que permitiua os participantes passar da experiência de cuidar de crianças comTEA de alta funcionalidade para as melhorias que poderiam ser aplicadas na saúde.CONCLUSÃO: Ofacto deter uma criança comTEA é um processo complexo,que gera diversas emoções nos pais,mas que a pesar disso puderam viver um processo de adaptação,maior conhecimento teórico e prático da condição, permitindo um crescimento e desenvolvimento favorável para a criança e para as famílias