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1.
Trends Biochem Sci ; 43(9): 650-653, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-29729937

RESUMEN

An emerging application of mid-IR spectrochemical imaging of the retina is its utility in studying the highly localized biomolecular alterations in the chemistry of retinal cell layers associated with several pathological conditions. Spatially resolved IR images highlight simultaneous chemical composition of the entire span of the retina in a label-free manner.


Asunto(s)
Diagnóstico por Imagen/métodos , Rayos Infrarrojos , Retina/diagnóstico por imagen , Retina/metabolismo , Animales , Humanos , Espectrofotometría Infrarroja
2.
J Chem Phys ; 150(15): 154124, 2019 Apr 21.
Artículo en Inglés | MEDLINE | ID: mdl-31005105

RESUMEN

Midinfrared absorbance spectra obtained from spatially inhomogeneous and finite samples often contain scattering effects characterized by derivative-like bands with shifted peak positions. Such features may be interpreted and accurately modeled by Fano theory when the imaginary part of the complex dielectric function is small and Lorentzian in nature-as is the case for many biological media. Furthermore, by fitting Fano line shapes to isolated absorbance bands, recovery of the peak position and pure absorption strength can be obtained with high accuracy. Additionally, for small and optically soft spherical scatterers, recovery of one or the other of constant refractive index or radius (given approximate knowledge of the other) is possible.

3.
Analyst ; 143(19): 4674-4683, 2018 Sep 24.
Artículo en Inglés | MEDLINE | ID: mdl-30176033

RESUMEN

Short-term acclimation response of individual cells of Thalassiosira weissflogii was monitored by Synchrotron FTIR imaging over the span of 75 minutes. The cells, collected from batch cultures, were maintained in a constant flow of medium, at an irradiance of 120 µmol m-2 s-1 and at 20 °C. Multiple internal reflections due to the micro fluidic channel were modeled, and showed that fringes are additive sinusoids to the pure absorption of the other components of the system. Preprocessing of the hyperspectral cube (x, y, Abs(λ)) included removing spectral fringe using an EMSC approach. Principal component analysis of the time series of hyperspectral cubes showed macromolecular pool variations (carbohydrates, lipids and DNA/RNA) of less than 2% after fringe correction.

4.
Analyst ; 142(7): 1061-1072, 2017 Mar 27.
Artículo en Inglés | MEDLINE | ID: mdl-28210739

RESUMEN

Diabetic retinopathy is a microvascular complication of diabetes that can lead to blindness. In the present study, we aimed to determine the nature of diabetes-induced, highly localized biochemical changes in the neuroretina at the onset of diabetes. High-resolution synchrotron Fourier transform infrared (s-FTIR) wide field microscopy coupled with multivariate analysis (PCA-LDA) was employed to identify biomarkers of diabetic retinopathy with spatial resolution at the cellular level. We compared the retinal tissue prepared from 6-week-old Ins2Akita/+ heterozygous (Akita/+, N = 6; a model of diabetes) male mice with the wild-type (control, N = 6) mice. Male Akita/+ mice become diabetic at 4-weeks of age. Significant differences (P < 0.001) in the presence of biomarkers associated with diabetes and segregation of spectra were achieved. Differentiating IR bands attributed to nucleic acids (964, 1051, 1087, 1226 and 1710 cm-1), proteins (1662 and 1608 cm-1) and fatty acids (2854, 2923, 2956 and 3012 cm-1) were observed between the Akita/+ and the WT samples. A comparison between distinctive layers of the retina, namely the photoreceptor retinal layer (PRL), outer plexiform layer (OPL), inner nucleus layer (INL) and inner plexiform layer (IPL) suggested that the photoreceptor layer is the most susceptible layer to oxidative stress in short-term diabetes. Spatially-resolved chemical images indicated heterogeneities and oxidative-stress induced alterations in the diabetic retina tissue morphology compared with the WT retina. In this study, the spectral biomarkers and the spatial biochemical alterations in the diabetic retina and in specific layers were identified for the first time. We believe that the conclusions drawn from these studies will help to bridge the gap in our understanding of the molecular and cellular mechanisms that contribute to the pathobiology of diabetic retinopathy.


Asunto(s)
Retinopatía Diabética/diagnóstico por imagen , Estrés Oxidativo , Espectroscopía Infrarroja por Transformada de Fourier , Sincrotrones , Animales , Biomarcadores/análisis , Diabetes Mellitus Experimental/complicaciones , Masculino , Ratones , Ratones Endogámicos C57BL , Análisis Multivariante , Retina/fisiopatología
5.
Analyst ; 142(4): 660-669, 2017 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-28133664

RESUMEN

We have used thermal source Fourier Transform Infrared (FTIR) microtomographic imaging to compare sea ice diatoms growing under different light conditions. A prototype tomography accessory was designed to have sufficient degrees of freedom to align any tilted cylindrical sample relative to the axis of rotation, minimizing the off-axis path traced during rotation. The lightweight device rests on the motorized stage to position the sample in the field-of-view and enable mosaic imaging. Reconstruction routines were tested with simulated and real phantoms, to assess limitations in the Radon back-projection method employed. The distribution and abundance of biochemicals is analysed for targets larger than a single FPA tile. Two and three dimensional (2D and 3D) FTIR spectrochemical images were obtained with a Focal Plane Array (FPA, nominal 1.1 µm pixel edges) for phantoms (polystyrene beads in polyvinyl alcohol matrix) and diatom cells harvested from land fast, first-year ice sites in Resolute Passage (74 43.628'N; 95 33.330'W) and Dease Strait (69° 1.11'N; 105° 21.29'W), Nunavut, Canada. The analysis of relative concentrations of organic matter within the encapsulating silica frustules of diatoms is important for a better understanding of both the physiological state and the individual cellular response to environmental pressures. Analysis of 3D FTIR images of Nitzschia frigida collected from beneath high (17-19 cm) and low (3-7 cm) snow depth revealed higher concentrations of lipids in diatoms collected under low snow cover, uniquely based on spectroscopically determined total 3D cell volume and biochemical content.

6.
Proc Natl Acad Sci U S A ; 111(50): 17743-8, 2014 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-25385610

RESUMEN

The preservation of our cultural heritage is of great importance to future generations. Despite this, significant problems have arisen with the conservation of waterlogged wooden artifacts. Three major issues facing conservators are structural instability on drying, biological degradation, and chemical degradation on account of Fe(3+)-catalyzed production of sulfuric and oxalic acid in the waterlogged timbers. Currently, no conservation treatment exists that effectively addresses all three issues simultaneously. A new conservation treatment is reported here based on a supramolecular polymer network constructed from natural polymers with dynamic cross-linking formed by a combination of both host-guest complexation and a strong siderophore pendant from a polymer backbone. Consequently, the proposed consolidant has the ability to chelate and trap iron while enhancing structural stability. The incorporation of antibacterial moieties through a dynamic covalent linkage into the network provides the material with improved biological resistance. Exploiting an environmentally compatible natural material with completely reversible chemistries is a safer, greener alternative to current strategies and may extend the lifetime of many culturally relevant waterlogged artifacts around the world.

7.
Nat Methods ; 10(9): 861-4, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-23913258

RESUMEN

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).


Asunto(s)
Procesamiento de Imagen Asistido por Computador/métodos , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Microtomografía por Rayos X/métodos , Animales , Células Madre Embrionarias/ultraestructura , Cabello/ultraestructura , Humanos , Imagenología Tridimensional , Ratones , Populus/ultraestructura , Sincrotrones , Madera/ultraestructura
8.
J Biol Chem ; 289(49): 34241-9, 2014 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-25271163

RESUMEN

Fourier transform infrared spectromicroscopy provides label-free imaging to detect the spatial distribution of the characteristic functional groups in proteins, lipids, phosphates, and carbohydrates simultaneously in individual DRG neurons. We have identified ring-shaped distributions of lipid and/or carbohydrate enrichment in subpopulations of neurons which has never before been reported. These distributions are ring-shaped within the cytoplasm and are likely representative of the endoplasmic reticulum. The prevalence of chemical ring subtypes differs between large- and small-diameter neurons. Peripheral inflammation increased the relative lipid content specifically in small-diameter neurons, many of which are nociceptive. Because many small-diameter neurons express an ion channel involved in inflammatory pain, transient receptor potential ankyrin 1 (TRPA1), we asked whether this increase in lipid content occurs in TRPA1-deficient (knock-out) neurons. No statistically significant change in lipid content occurred in TRPA1-deficient neurons, indicating that the inflammation-mediated increase in lipid content is largely dependent on TRPA1. Because TRPA1 is known to mediate mechanical and cold sensitization that accompanies peripheral inflammation, our findings may have important implications for a potential role of lipids in inflammatory pain.


Asunto(s)
Carbohidratos/química , Ganglios Espinales/química , Inflamación/metabolismo , Lípidos/química , Dolor/metabolismo , Células Receptoras Sensoriales/química , Canales de Potencial de Receptor Transitorio/genética , Animales , Tamaño de la Célula , Adyuvante de Freund , Ganglios Espinales/metabolismo , Ganglios Espinales/ultraestructura , Expresión Génica , Miembro Posterior , Inflamación/inducido químicamente , Inflamación/genética , Inflamación/patología , Inyecciones Subcutáneas , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Dolor/inducido químicamente , Dolor/genética , Dolor/patología , Cultivo Primario de Células , Células Receptoras Sensoriales/metabolismo , Células Receptoras Sensoriales/ultraestructura , Espectroscopía Infrarroja por Transformada de Fourier , Canal Catiónico TRPA1 , Canales de Potencial de Receptor Transitorio/deficiencia
9.
Analyst ; 140(12): 4046-56, 2015 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-26000346

RESUMEN

The chemical status of live sensory neurons is accessible with infrared microspectroscopy of appropriately prepared cells. In this paper, individual dorsal root ganglion (DRG) neurons have been prepared with two different protocols, and plated on glass cover slips, BaF2 and CaF2 substrates. The first protocol exposes the intact DRGs to 4 °C for between 20-30 minutes before dissociating individual neurons and plating 2 hours later. The second protocol maintains the neurons at 23 °C for the entire duration of the sample preparation. The visual appearance of the neurons is similar. The viability was assessed by means of trypan blue exclusion method to determine the viability of the neurons. The neurons prepared under the first protocol (cold exposure) and plated on BaF2 reveal a distinct chemical signature and chemical distribution that is different from the other sample preparations described in the paper. Importantly, results for other sample preparation methods, using various substrates and temperature protocols, when compared across the overlapping spectral bandwidth, present normal chemical distribution within the neurons. The unusual chemically specific spatial variation is dominated by a lack of protein and carbohydrates in the center of the neurons and signatures of unraveling DNA are detected. We suggest that cold shock leads to apoptosis of DRGs, followed by osmotic stress originating from ion gradients across the cell membrane leading to cell lysis.


Asunto(s)
Apoptosis , Respuesta al Choque por Frío , Ganglios Espinales/citología , Rayos Infrarrojos , Neuronas/citología , Animales , Fluoruro de Calcio/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Neuronas/metabolismo , Análisis de Componente Principal , Espectroscopía Infrarroja por Transformada de Fourier
10.
Analyst ; 140(18): 6421, 2015 Sep 21.
Artículo en Inglés | MEDLINE | ID: mdl-26273705

RESUMEN

Correction for 'Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows' by Ebrahim Aboualizadeh et al., Analyst, 2015, 140, 4046-4056.

11.
Anal Chem ; 86(15): 7516-22, 2014 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-24992696

RESUMEN

Herein, we report on a facile, low-cost, and efficient method to tune the structure and properties of chemically reduced graphene oxide (rGO) by applying a transient voltage across the rGO for ultrasensitive gas sensors. A large number of defects, including pits, are formed in the rGO upon the voltage activation. More interestingly, the number of epoxide and ether functional groups in the rGO increased after the voltage activation. The voltage-activated rGO was highly sensitive to NO2 with a sensitivity 500% higher than that of the original rGO. The lower detection limit can reach an unprecedented ultralow concentration of 50 ppb for NO2 sensing. Density functional theory (DFT) calculations revealed that the high sensitivity to NO2 is attributed to the efficient charge transfer from ether groups to NO2, which is the dominant sensing mechanism. This study points to a promising method to tune the properties of graphene-based materials through the creation of additional defects and functional groups for high-performance gas sensors.

12.
Nat Methods ; 8(5): 413-6, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21423192

RESUMEN

Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.


Asunto(s)
Microscopía/métodos , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Sincrotrones , Mama/anatomía & histología , Femenino , Humanos , Procesamiento de Imagen Asistido por Computador/métodos , Procesamiento de Imagen Asistido por Computador/estadística & datos numéricos , Masculino , Microscopía/instrumentación , Neoplasias de la Próstata/patología , Espectroscopía Infrarroja por Transformada de Fourier/instrumentación , Espectroscopía Infrarroja por Transformada de Fourier/estadística & datos numéricos , Coloración y Etiquetado
13.
Analyst ; 139(19): 4769-74, 2014 Oct 07.
Artículo en Inglés | MEDLINE | ID: mdl-25055796

RESUMEN

New methods are needed to rapidly identify malaria parasites in blood smears. The coupling of a Focal Plane Array (FPA) infrared microscope system to a synchrotron light source at IRENI enables rapid molecular imaging at high spatial resolution. The technique, in combination with hyper-spectral processing, enables imaging and diagnosis of early stage malaria parasites at the single cell level in a blood smear. The method relies on the detection of distinct lipid signatures associated with the different stages of the malaria parasite and utilises resonant Mie extended multiplicative scatter correction to pre-process the spectra followed by full bandwidth image deconvolution to resolve the single cells. This work demonstrates the potential of focal plane technology to diagnose single cells in a blood smear. Brighter laboratory based infrared sources, optical refinements and higher sensitive detectors will soon see the emergence of focal plane array imaging in the clinical environment.


Asunto(s)
Malaria/diagnóstico , Fotomicrografía , Espectroscopía Infrarroja por Transformada de Fourier , Eritrocitos/citología , Eritrocitos/parasitología , Humanos , Procesamiento de Imagen Asistido por Computador , Malaria/parasitología , Plasmodium falciparum/inmunología , Plasmodium falciparum/aislamiento & purificación , Análisis de Componente Principal , Análisis de la Célula Individual , Análisis de Matrices Tisulares
14.
J Synchrotron Radiat ; 20(Pt 3): 482-9, 2013 May.
Artículo en Inglés | MEDLINE | ID: mdl-23592628

RESUMEN

The wings of some insects, such as cicadae, have been reported to possess a number of interesting and unusual qualities such as superhydrophobicity, anisotropic wetting and antibacterial properties. Here, the chemical composition of the wings of the Clanger cicada (Psaltoda claripennis) were characterized using infrared (IR) microspectroscopy. In addition, the data generated from two separate synchrotron IR facilities, the Australian Synchrotron Infrared Microspectroscopy beamline (AS-IRM) and the Synchrotron Radiation Center (SRC), University of Wisconsin-Madison, IRENI beamline, were analysed and compared. Characteristic peaks in the IR spectra of the wings were assigned primarily to aliphatic hydrocarbon and amide functionalities, which were considered to be an indication of the presence of waxy and proteinaceous components, respectively, in good agreement with the literature. Chemical distribution maps showed that, while the protein component was homogeneously distributed, a significant degree of heterogeneity was observed in the distribution of the waxy component, which may contribute to the self-cleaning and aerodynamic properties of the cicada wing. When comparing the data generated from the two beamlines, it was determined that the SRC IRENI beamline was capable of producing higher-spatial-resolution distribution images in a shorter time than was achievable at the AS-IRM beamline, but that spectral noise levels per pixel were considerably lower on the AS-IRM beamline, resulting in more favourable data where the detection of weak absorbances is required. The data generated by the two complementary synchrotron IR methods on the chemical composition of cicada wings will be immensely useful in understanding their unusual properties with a view to reproducing their characteristics in, for example, industry applications.


Asunto(s)
Hemípteros/química , Espectrofotometría Infrarroja/métodos , Sincrotrones , Termografía/métodos , Alas de Animales/química , Animales , Interacciones Hidrofóbicas e Hidrofílicas , Propiedades de Superficie
15.
Analyst ; 138(14): 3991-7, 2013 Jul 21.
Artículo en Inglés | MEDLINE | ID: mdl-23586070

RESUMEN

While the basis of neuronal degeneration in Alzheimer's disease (AD) continues to be debated, the amyloid cascade hypothesis remains central. Amyloid plaques are a required pathological marker for post mortem diagnosis, and Aß peptide is regarded by most as a critical trigger at the very least. We present spectrochemical image analysis of brain tissue sections obtained with the mid-infrared beamline IRENI (InfraRed ENvironmental Imaging, Synchrotron Radiation Center, U Wisconsin-Madison), where the pixel resolution of 0.54 × 0.54 µm(2) permits analysis at sub-cellular dimensions. Spectrochemical images of dense core plaque found in hippocampus and cortex sections of two transgenic mouse models of AD (TgCRND8 and 3×Tg) are compared with plaque images from a 91 year old apoE43 human AD case. Spectral analysis was done in conjunction with histochemical stains of serial sections. A lipid membrane-like spectral signature surrounded and infiltrated the dense core plaques in all cases. Remarkable compositional similarities in early stage plaques suggest similar routes to plaque formation, regardless of genetic predisposition or mammalian origin.


Asunto(s)
Enfermedad de Alzheimer/patología , Corteza Cerebral/patología , Hipocampo/patología , Lípidos/análisis , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Sincrotrones , Anciano de 80 o más Años , Enfermedad de Alzheimer/genética , Animales , Autopsia , Modelos Animales de Enfermedad , Femenino , Humanos , Ratones , Ratones Transgénicos , Placa Amiloide
16.
Analyst ; 138(1): 240-8, 2013 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-23152953

RESUMEN

Understanding stem cell (SC) biology remains challenging and one of the few human tissues within which their in situ location is well characterized is the cornea. Individual human corneal epithelial cells were isolated from biopsies of live tissues using fluorescence-activated cell sorting (FACS); these were divided into putative SCs, transit-amplifying (TA) cells and terminally-differentiated (TD) cells. Employing synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy with a focal plane array (FPA), sub-cellular spatial resolution analysis of unstained isolated cells was achieved as a consequence of the brilliance of a 12 collimated beams arrangement allowing rapid spectral acquisition. Infrared (IR) spectra were extracted and pre-processed. Subsequent categorization with multivariate analysis of IR spectra derived from FPA images was used to investigate biomolecular changes between classes. A progressive segregation in cell-specific spectral categories with differentiation from SC to TA cell to TD cell was noted. Multiple different absorption peaks that discriminated putative SCs, TA cells and TD cells across DNA, protein and lipid spectral regions were identified. DNA regions (1080 and 1225 cm(-1)) and some protein regions (1443 cm(-1)) primarily segregated SCs from TA cells and TD cells, whilst amide regions and lipids (1,550, 1650 and 1740 cm(-1)) segregated TA cells and TD cells. Scanning electron microscopy images verified the external phenotypic characteristics of the different isolated cell types. These findings highlight the applicability of SR-FTIR microspectroscopy towards distinguishing SCs, TA cells and TD cells, and suggest that cellular classification via traditional methods of immunolabelling can be greatly aided by the use of spectral biomarkers.


Asunto(s)
Córnea/citología , Espacio Intracelular/metabolismo , Microtecnología/instrumentación , Imagen Molecular/instrumentación , Espectroscopía Infrarroja por Transformada de Fourier/instrumentación , Sincrotrones , Separación Celular , Análisis Discriminante , Humanos , Análisis de Componente Principal
17.
Analyst ; 138(19): 5610-8, 2013 Oct 07.
Artículo en Inglés | MEDLINE | ID: mdl-23826609

RESUMEN

Advancements in widefield infrared spectromicroscopy have recently been demonstrated following the commissioning of IRENI (InfraRed ENvironmental Imaging), a Fourier Transform infrared (FTIR) chemical imaging beamline at the Synchrotron Radiation Center. The present study demonstrates the effects of magnification, spatial oversampling, spectral pre-processing and deconvolution, focusing on the intracellular detection and distribution of an exogenous metal tris-carbonyl derivative 1 in a single MDA-MB-231 breast cancer cell. We demonstrate here that spatial oversampling for synchrotron-based infrared imaging is critical to obtain accurate diffraction-limited images at all wavelengths simultaneously. Resolution criteria and results from raw and deconvoluted images for two Schwarzschild objectives (36×, NA 0.5 and 74×, NA 0.65) are compared to each other and to prior reports for raster-scanned, confocal microscopes. The resolution of the imaging data can be improved by deconvolving the instrumental broadening that is determined with the measured PSFs, which is implemented with GPU programming architecture for fast hyperspectral processing. High definition, rapidly acquired, FTIR chemical images of respective spectral signatures of the cell 1 and shows that 1 is localized next to the phosphate- and Amide-rich regions, in agreement with previous infrared and luminescence studies. The infrared image contrast, localization and definition are improved after applying proven spectral pre-processing (principal component analysis based noise reduction and RMie scattering correction algorithms) to individual pixel spectra in the hyperspectral cube.


Asunto(s)
Líquido Intracelular/química , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Línea Celular Tumoral , Femenino , Humanos
18.
Microsc Microanal ; 19(4): 821-34, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23745591

RESUMEN

Nanoparticles composed of a magnetic iron oxide core surrounded by a metal shell have utility in a broad range of biomedical applications. However, the presence of surface energy differences between the two components makes wetting of oxide with metal unfavorable, precluding a "core-shell" structure of an oxide core completely surrounded by a thin metal shell. Three-dimensional island growth followed by island coalescence into thick shells is favored over the two-dimensional layer-by-layer growth of a thin, continuous metal coating of a true core-shell. Aqueous synthesis of gold-coated magnetite nanoparticles with analysis by infrared, energy-dispersive X-ray, and electron energy loss spectroscopies; high-resolution transmission electron microscopy; selected area electron diffraction; and high-angle annular dark-field scanning transmission electron microscopy showed two distinct morphologies that are inconsistent with an idealized core-shell. The majority were isolated ~16-22-nm-diameter nanoparticles consisting of ~7-nm-diameter magnetite and a thick deposition of gold, most often discontinuous, with some potentially "sandwiched" morphologies. A minority were aggregates of agglomerated magnetite decorated with gold but displaying significant bare magnetite. Both populations were successfully conjugated to fibrinogen and targeted to surface-activated platelets, demonstrating that iron oxide-gold nanoparticles produced by aqueous synthesis do not require an ideal core-shell structure for biological activity in cell labeling and targeting applications.


Asunto(s)
Plaquetas/metabolismo , Óxido Ferrosoférrico/metabolismo , Fibrinógeno/metabolismo , Oro/metabolismo , Nanopartículas/metabolismo , Óxido Ferrosoférrico/química , Fibrinógeno/química , Oro/química , Humanos , Nanopartículas/química , Unión Proteica
19.
Int J Mol Sci ; 14(11): 22753-81, 2013 Nov 19.
Artículo en Inglés | MEDLINE | ID: mdl-24256815

RESUMEN

Infrared (IR) spectromicroscopy, or chemical imaging, is an evolving technique that is poised to make significant contributions in the fields of biology and medicine. Recent developments in sources, detectors, measurement techniques and speciman holders have now made diffraction-limited Fourier transform infrared (FTIR) imaging of cellular chemistry in living cells a reality. The availability of bright, broadband IR sources and large area, pixelated detectors facilitate live cell imaging, which requires rapid measurements using non-destructive probes. In this work, we review advances in the field of FTIR spectromicroscopy that have contributed to live-cell two and three-dimensional IR imaging, and discuss several key examples that highlight the utility of this technique for studying the structure and chemistry of living cells.


Asunto(s)
Rastreo Celular , Imagenología Tridimensional , Espectrofotometría Infrarroja , Espectroscopía Infrarroja por Transformada de Fourier , Rastreo Celular/instrumentación , Rastreo Celular/métodos , Humanos , Imagenología Tridimensional/instrumentación , Imagenología Tridimensional/métodos , Sustancias Macromoleculares/química , Sustancias Macromoleculares/ultraestructura , Espectrofotometría Infrarroja/instrumentación , Espectrofotometría Infrarroja/métodos , Espectroscopía Infrarroja por Transformada de Fourier/instrumentación , Espectroscopía Infrarroja por Transformada de Fourier/métodos
20.
Anal Chem ; 84(14): 6173-80, 2012 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-22732086

RESUMEN

Fourier transform infrared (FTIR) microspectroscopy is a powerful technique for label-free chemical imaging that has supplied important chemical information about heterogeneous samples for many problems across a variety of disciplines. State-of-the-art synchrotron based infrared (IR) microspectrometers can yield high-resolution images, but are truly diffraction limited for only a small spectral range. Furthermore, a fundamental trade-off exists between the number of pixels, acquisition time and the signal-to-noise ratio, limiting the applicability of the technique. The recently commissioned infrared synchrotron beamline, infrared environmental imaging (IRENI), overcomes this trade off and delivers 4096-pixel diffraction limited IR images with high signal-to-noise ratio in under a minute. The spatial oversampling for all mid-IR wavelengths makes the IRENI data ideal for spatial image restoration techniques. Here, we measured and fitted wavelength-dependent point-spread-functions (PSFs) at IRENI for a 74× objective between the sample plane and detector. Noise-free wavelength-dependent theoretical PSFs are deconvoluted from images generated from narrow bandwidths (4 cm(-1)) over the entire mid-infrared range (4000-900 cm(-1)). The stack of restored images is used to reconstruct the spectra. Restored images of metallic test samples with features that are 2.5 µm and smaller are clearly improved in comparison to the raw data images for frequencies above 2000 cm(-1). Importantly, these spatial image restoration methods also work for samples with vibrational bands in the recorded mid-IR fingerprint region (900-1800 cm(-1)). Improved signal-to-noise spectra are reconstructed from the restored images as demonstrated for a mixture of spherical polystyrene beads in a polyurethane matrix. Finally, a freshly thawed retina tissue section is used to demonstrate the success of deconvolution achievable with a heterogeneous, irregularly shaped, biologically relevant sample with distinguishing spectroscopic features across the entire mid-IR spectral range.


Asunto(s)
Espectrofotometría Infrarroja/métodos , Estadística como Asunto/métodos , Animales , Ratones , Polímeros/química , Retina/citología
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