RESUMEN
The endoplasmic reticulum (ER) is a eukaryotic organelle that plays important roles in protein synthesis, folding and trafficking, calcium homoeostasis and lipid and steroid synthesis. It is the major protein synthesis compartment for secreted, plasma membrane and organelle proteins. Perturbations of ER homeostasis such as the accumulation of unfolded or misfolded proteins cause ER stress. To alleviate this stress, ER triggers an evolutionarily conserved signalling cascade called the unfolded protein response (UPR). As an initial response, the UPR aims at adapting and restoring ER function by translational attenuation, upregulation of ER chaperones and degradation of unfolded proteins. However, if the ER function is severely impaired because of excessive or prolonged exposure to stress, then the inflicted cells may undergo programmed cell death. During ER stress, unstable or partially folded mutant proteins are prevented from trafficking to their proper subcellular localizations and usually rapidly degraded. The small molecules named chemical chaperones help to stabilize these mutant proteins and facilitate their folding and proper trafficking from the ER to their final destinations. Because increasing number of studies suggest that ER stress is involved in a number of disease pathogenesis including neurodegenerative diseases, cancer, obesity, diabetes and atherosclerosis, promoting ER folding capacity through chemical chaperones emerges as a novel therapeutic approach. In this review, we provide insight into the many important functions of chemical chaperones during ER stress, their impact on the ER-stress-related pathologies and their potential as a new drug targets, especially in the context of metabolic disorders.
Asunto(s)
Retículo Endoplásmico/fisiología , Enfermedades Metabólicas/terapia , Chaperonas Moleculares/fisiología , Respuesta de Proteína Desplegada/fisiología , Apoptosis/fisiología , Homeostasis/fisiología , Humanos , Enfermedades Metabólicas/etiología , Biosíntesis de Proteínas/fisiología , Pliegue de Proteína , Transducción de Señal/fisiología , Estrés FisiológicoRESUMEN
Tumor necrosis factor-alpha (TNF-alpha) has been shown to have certain catabolic effects on fat cells and whole animals. An induction of TNF-alpha messenger RNA expression was observed in adipose tissue from four different rodent models of obesity and diabetes. TNF-alpha protein was also elevated locally and systemically. Neutralization of TNF-alpha in obese fa/fa rats caused a significant increase in the peripheral uptake of glucose in response to insulin. These results indicate a role for TNF-alpha in obesity and particularly in the insulin resistance and diabetes that often accompany obesity.
Asunto(s)
Tejido Adiposo/fisiopatología , Resistencia a la Insulina/genética , Obesidad/genética , Obesidad/fisiopatología , ARN Mensajero/biosíntesis , Factor de Necrosis Tumoral alfa/genética , Células 3T3 , Tejido Adiposo/fisiología , Animales , Glucemia/metabolismo , Northern Blotting , Diabetes Mellitus Experimental/fisiopatología , Técnica de Clampeo de la Glucosa , Homeostasis , Inmunoglobulina G/genética , Inmunoglobulina G/farmacología , Insulina/farmacología , Sistemas de Infusión de Insulina , Masculino , Ratones , Ratones Obesos , Obesidad/inducido químicamente , ARN/genética , ARN/aislamiento & purificación , ARN Mensajero/aislamiento & purificación , Ratas , Ratas Zucker , Receptores de Superficie Celular/genética , Receptores de Superficie Celular/fisiología , Receptores del Factor de Necrosis Tumoral , Proteínas Recombinantes de Fusión/farmacología , Valores de Referencia , Glutamato de Sodio , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
Genes that control the early stages of adipogenesis remain largely unknown. Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. This effect is mediated, at least in part, through the direct suppression of peroxisome proliferator-activated receptor gamma. GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition.
Asunto(s)
Adipocitos/citología , Adipocitos/metabolismo , Proteínas de Unión al ADN/metabolismo , Transactivadores/metabolismo , Factores de Transcripción/metabolismo , Células 3T3 , Tejido Adiposo/citología , Tejido Adiposo/metabolismo , Tejido Adiposo Pardo/citología , Tejido Adiposo Pardo/metabolismo , Animales , Diferenciación Celular , Células Cultivadas , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/genética , Factor de Transcripción GATA2 , Factor de Transcripción GATA3 , Expresión Génica , Ratones , Mutación , Obesidad/genética , Obesidad/metabolismo , Regiones Promotoras Genéticas , Receptores Citoplasmáticos y Nucleares/genética , Receptores Citoplasmáticos y Nucleares/metabolismo , Células Madre/citología , Transactivadores/química , Transactivadores/genética , Factores de Transcripción/química , Factores de Transcripción/genética , Transcripción Genética , Dedos de ZincRESUMEN
Fatty acid binding proteins (FABPs) are small cytoplasmic proteins that are expressed in a highly tissue-specific manner and bind to fatty acids such as oleic and retinoic acid. Mice with a null mutation in aP2, the gene encoding the adipocyte FABP, were developmentally and metabolically normal. The aP2-deficient mice developed dietary obesity but, unlike control mice, they did not develop insulin resistance or diabetes. Also unlike their obese wild-type counterparts, obese aP2-/- animals failed to express in adipose tissue tumor necrosis factor-alpha (TNF-alpha), a molecule implicated in obesity-related insulin resistance. These results indicate that aP2 is central to the pathway that links obesity to insulin resistance, possibly by linking fatty acid metabolism to expression of TNF-alpha.
Asunto(s)
Tejido Adiposo/metabolismo , Proteínas Portadoras/fisiología , Ácidos Grasos/metabolismo , Resistencia a la Insulina , Proteína P2 de Mielina/fisiología , Proteínas de Neoplasias , Proteínas del Tejido Nervioso , Obesidad/metabolismo , Factor de Necrosis Tumoral alfa/biosíntesis , Animales , Glucemia/metabolismo , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Grasas de la Dieta/administración & dosificación , Proteína de Unión a los Ácidos Grasos 7 , Proteínas de Unión a Ácidos Grasos , Femenino , Regulación de la Expresión Génica , Marcación de Gen , Prueba de Tolerancia a la Glucosa , Homeostasis , Insulina/sangre , Masculino , Ratones , Ratones Endogámicos C57BL , Mutación , Proteína P2 de Mielina/genética , Proteína P2 de Mielina/metabolismo , Triglicéridos/sangre , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Tumor necrosis factor-alpha (TNF-alpha) is an important mediator of insulin resistance in obesity and diabetes through its ability to decrease the tyrosine kinase activity of the insulin receptor (IR). Treatment of cultured murine adipocytes with TNF-alpha was shown to induce serine phosphorylation of insulin receptor substrate 1 (IRS-1) and convert IRS-1 into an inhibitor of the IR tyrosine kinase activity in vitro. Myeloid 32D cells, which lack endogenous IRS-1, were resistant to TNF-alpha-mediated inhibition of IR signaling, whereas transfected 32D cells that express IRS-1 were very sensitive to this effect of TNF-alpha. An inhibitory form of IRS-1 was observed in muscle and fat tissues from obese rats. These results indicate that TNF-alpha induces insulin resistance through an unexpected action of IRS-1 to attenuate insulin receptor signaling.
Asunto(s)
Adipocitos/metabolismo , Resistencia a la Insulina/fisiología , Obesidad/metabolismo , Fosfoproteínas/fisiología , Receptor de Insulina/antagonistas & inhibidores , Factor de Necrosis Tumoral alfa/farmacología , Tejido Adiposo/metabolismo , Animales , Células Cultivadas , Insulina/farmacología , Proteínas Sustrato del Receptor de Insulina , Masculino , Ratones , Músculo Esquelético/metabolismo , Fosfoproteínas/metabolismo , Fosforilación , Ratas , Ratas Zucker , Receptor de Insulina/metabolismo , Serina/metabolismo , Transducción de SeñalRESUMEN
Obesity is associated with chronic low-grade inflammation. Inflammatory signals interfere with insulin action and disrupt metabolic homeostasis. The c-Jun N-terminal kinase (JNK) has been identified as a central mediator of insulin resistance. Recent studies showed that in obesity compromising endoplasmic reticulum (ER) function results in insulin resistance and type 2 diabetes that are dependent on JNK activation. In contrast, enhancing ER function in transgenic mice or by the use of chemical chaperones protects against diet-induced insulin resistance. Hence, ER stress and the related signaling networks present a critical mechanism underlying obesity-induced JNK activity, inflammatory response and insulin resistance.
Asunto(s)
Diabetes Mellitus Tipo 2/fisiopatología , Retículo Endoplásmico/fisiología , Inflamación/fisiopatología , Resistencia a la Insulina/fisiología , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Obesidad/fisiopatología , Tejido Adiposo/enzimología , Animales , Retículo Endoplásmico/enzimología , Humanos , Proteínas Quinasas JNK Activadas por Mitógenos/genética , Ratones , Modelos Biológicos , Pliegue de Proteína , Estrés FisiológicoRESUMEN
Insulin resistance is an important metabolic abnormality often associated with infections, cancer, obesity, and especially non-insulin-dependent diabetes mellitus (NIDDM). We have previously demonstrated that tumor necrosis factor-alpha produced by adipose tissue is a key mediator of insulin resistance in animal models of obesity-diabetes. However, the mechanism by which TNF-alpha interferes with insulin action is not known. Since a defective insulin receptor (IR) tyrosine kinase activity has been observed in obesity and NIDDM, we measured the IR tyrosine kinase activity in the Zucker (fa/fa) rat model of obesity and insulin resistance after neutralizing TNF-alpha with a soluble TNF receptor (TNFR)-lgG fusion protein. This neutralization resulted in a marked increase in insulin-stimulated autophosphorylation of the IR, as well as phosphorylation of insulin receptor substrate 1 (IRS-1) in muscle and fat tissues of the fa/fa rats, restoring them to near control (lean) levels. In contrast, no significant changes were observed in insulin-stimulated tyrosine phosphorylations of IR and IRS-1 in liver. The physiological significance of the improvements in IR signaling was indicated by a concurrent reduction in plasma glucose, insulin, and free fatty acid levels. These results demonstrate that TNF-alpha participates in obesity-related systemic insulin resistance by inhibiting the IR tyrosine kinase in the two tissues mainly responsible for insulin-stimulated glucose uptake: muscle and fat.
Asunto(s)
Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus/metabolismo , Obesidad , Proteínas Tirosina Quinasas/metabolismo , Receptor de Insulina/metabolismo , Factor de Necrosis Tumoral alfa/fisiología , Tejido Adiposo/metabolismo , Animales , Diabetes Mellitus/enzimología , Diabetes Mellitus Tipo 2/inducido químicamente , Diabetes Mellitus Tipo 2/enzimología , Modelos Animales de Enfermedad , Inmunoglobulina G/genética , Insulina/sangre , Insulina/farmacología , Proteínas Sustrato del Receptor de Insulina , Resistencia a la Insulina , Hígado/metabolismo , Masculino , Músculos/metabolismo , Pruebas de Neutralización , Fosfoproteínas/metabolismo , Fosforilación , Fosfotirosina , Ratas , Ratas Zucker , Receptores del Factor de Necrosis Tumoral/genética , Proteínas Recombinantes de Fusión/farmacología , Organismos Libres de Patógenos Específicos , Tirosina/análogos & derivados , Tirosina/análisisRESUMEN
Obesity is frequently associated with insulin resistance and abnormal glucose homeostasis. Recent studies in animal models have indicated that TNF-alpha plays an important role in mediating the insulin resistance of obesity through its overexpression in fat tissue. However, the mechanisms linking obesity to insulin resistance and diabetes in humans remain largely unknown. In this study we examined the expression pattern of TNF-alpha mRNA in adipose tissues from 18 control and 19 obese premenopausal women by Northern blot analysis. TNF-alpha protein concentrations in plasma and in conditioned medium of explanted adipose tissue were measured by ELISA. Furthermore, the effects of weight reduction by dietary treatment of obesity on the adipose expression of TNF-alpha mRNA were also analyzed in nine premenopausal obese women, before and after a controlled weight-reduction program. These studies demonstrated that obese individuals express 2.5-fold more TNF-alpha mRNA in fat tissue relative to the lean controls (P < 0.001). Similar increases were also observed in adipose production of TNF-alpha protein but circulating TNF-alpha levels were extremely low or undetectable. A strong positive correlation was observed between TNF-alpha mRNA expression levels in fat tissue and the level of hyperinsulinemia (P < 0.001), an indirect measure of insulin resistance. Finally, body weight reduction in obese subjects which resulted in improved insulin sensitivity was also associated with a decrease in TNF-alpha mRNA expression (45%, P < 0.001) in fat tissue. These results suggest a role for the abnormal regulation of this cytokine in the pathogenesis of obesity-related insulin resistance.
Asunto(s)
Tejido Adiposo/metabolismo , Expresión Génica , Resistencia a la Insulina , Obesidad/metabolismo , Factor de Necrosis Tumoral alfa/biosíntesis , Adulto , Glucemia/metabolismo , Northern Blotting , Índice de Masa Corporal , Colesterol/sangre , Femenino , Humanos , Insulina/sangre , Persona de Mediana Edad , Premenopausia , ARN Mensajero/biosíntesis , Valores de Referencia , Análisis de Regresión , Delgadez/metabolismo , Triglicéridos/sangre , Factor de Necrosis Tumoral alfa/análisisRESUMEN
Cytokines, in particular tumor necrosis factor-alpha (TNF-alpha), have significant effects on energy metabolism and appetite although their mechanisms of action are largely unknown. Here, we examined whether TNF-alpha modulates the production of leptin, the recently identified fat-specific energy balance hormone, in cultured adipocytes and in mice. TNF-alpha treatment of 3T3-L1 adipocytes resulted in rapid stimulation of leptin accumulation in the media, with a maximum effect at 6 h. This stimulation was insensitive to cycloheximide, a protein synthesis inhibitor, but was completely inhibited by the secretion inhibitor brefeldin A, indicating a posttranslational effect. Treatment of mice with TNF-alpha also caused a similar increase in plasma leptin levels. Finally, in obese TNF-alpha-deficient mice, circulating leptin levels were significantly lower, whereas adipose tissue leptin was higher compared with obese wild-type animals. These data provide evidence that TNF-alpha can act directly on adipocytes to regulate the release of a preformed pool of leptin. Furthermore, they suggest that the elevated adipose tissue expression of TNF-alpha that occurs in obesity may contribute to obesity-related hyperleptinemia.
Asunto(s)
Adipocitos/metabolismo , Tejido Adiposo/metabolismo , Obesidad/metabolismo , Proteínas/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Adipocitos/efectos de los fármacos , Animales , Línea Celular , Células Cultivadas , Leptina , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones Obesos , Biosíntesis de Proteínas , Proteínas/genética , ARN Mensajero/metabolismo , Proteínas Recombinantes/farmacología , Factor de Necrosis Tumoral alfa/deficiencia , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Uncoupling protein 2 (UCP2) has been proposed to play a prominent role in the regulation of energy balance. UCP2 mRNA expression is upregulated in white adipose tissue (WAT) and liver, but is not altered in skeletal muscle in genetically obese ob/ob mice. The mechanisms involved in the upregulation of UCP2 in obesity have not been investigated. We have now examined the potential role of leptin, hyperphagia, increased tissue lipid content, and overexpression of tumor necrosis factor (TNF)-alpha in the upregulation of UCP2 mRNA expression in the liver and WAT in ob/ob mice. Treatment of ob/ob mice with leptin for 3 days significantly reduced their food intake but had no effect on the upregulation of UCP2 mRNA levels in the liver or WAT. To investigate the effect of feeding and higher tissue lipid content on the upregulation of UCP2 in liver and WAT, we compared UCP2 mRNA levels in ad-libitum fed and 72-h fasted control and ob/ob mice. In controls, fasting had no effect on UCP2 mRNA levels in liver, but increased UCP2 mRNA in WAT suggesting that the effects of fasting on UCP2 mRNA levels are tissue-specific. In ob/ob mice, fasting did not lower UCP2 mRNA levels in liver or WAT suggesting that the upregulation of UCP2 in ob/ob mice is not merely a direct consequence of increased food intake. 72-h fasting lowered hepatic total lipid content by 34% and 36% in control and ob/ob mice, respectively, without any corresponding decrease in hepatic UCP2 mRNA levels, suggesting that the enhanced UCP2 expression in the liver of ob/ob mice is not secondary to lipid accumulation in their livers. Although TNF-alpha has been shown to acutely increase UCP2 mRNA levels in liver and WAT, and is overexpressed in adipose tissue in obesity, deletion of the genes for both TNF receptors in ob/ob mice produces a further increase in UCP2 mRNA expression in liver and adipose tissue indicating a paradoxical inhibitory role. Taken together, these results suggest that the upregulation of UCP2 mRNA levels in the liver and WAT of ob/ob mice is not due to the lack of leptin, hyperphagia, increased tissue lipid content, or over-expression of TNF-alpha.
Asunto(s)
Proteínas de Transporte de Membrana , Proteínas Mitocondriales , Obesidad/genética , Proteínas/genética , Tejido Adiposo/química , Tejido Adiposo/metabolismo , Animales , Alimentos , Privación de Alimentos , Hiperfagia/fisiopatología , Canales Iónicos , Leptina/farmacología , Metabolismo de los Lípidos , Lípidos/análisis , Hígado/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Obesidad/metabolismo , ARN Mensajero/análisis , Factor de Necrosis Tumoral alfa/farmacología , Proteína Desacopladora 2 , Regulación hacia ArribaRESUMEN
Recent data have suggested a key role for tumor necrosis factor (TNF)-alpha in the insulin resistance of obesity and non-insulin-dependent diabetes mellitus (NIDDM). TNF-alpha expression is elevated in the adipose tissue of multiple experimental models of obesity. Neutralization of TNF-alpha in one of these models improves insulin sensitivity by increasing the activity of the insulin receptor tyrosine kinase, specifically in muscle and fat tissues. On a cellular level, TNF-alpha is a potent inhibitor of the insulin-stimulated tyrosine phosphorylations on the beta-chain of the insulin receptor and insulin receptor substrate-1, suggesting a defect at or near the tyrosine kinase activity of the insulin receptor. Given the clear link between obesity, insulin resistance, and diabetes, these results strongly suggest that TNF-alpha may play a crucial role in the systemic insulin resistance of NIDDM. This may allow for new treatments of disorders involving resistance to insulin.
Asunto(s)
Diabetes Mellitus Tipo 2/fisiopatología , Diabetes Mellitus/fisiopatología , Obesidad , Factor de Necrosis Tumoral alfa/fisiología , Tejido Adiposo/fisiopatología , Animales , Humanos , Resistencia a la Insulina/fisiologíaRESUMEN
Previous studies have shown that tumor necrosis factor (TNF)-alpha production from adipose tissue is elevated in rodent and human obesity and plays an important role in insulin resistance in experimental animal models. In this study, we examined the adipose expression of both TNF receptors (TNFR1 and TNFR2) in human obesity and demonstrated that obese female subjects express approximately twofold more TNFR2 mRNA in fat tissue and approximately sixfold more soluble TNFR2 in circulation relative to lean control subjects. In contrast, TNFR1 expression and protein levels were similar in these subjects. TNFR2 expression levels in adipose tissue were strongly correlated with BMI (r = 0.65, P < 0.001) and level of hyperinsulinemia (P < 0.001), an indirect measure of insulin resistance, as well as level of TNF-alpha mRNA expression in fat tissue (r = 0.56, P < 0.001). These results suggest that TNFR2 might play a role in human obesity by modulating the actions of TNF-alpha.
Asunto(s)
Tejido Adiposo/metabolismo , Antígenos CD/biosíntesis , Regulación de la Expresión Génica , Resistencia a la Insulina , Obesidad/metabolismo , Receptores del Factor de Necrosis Tumoral/biosíntesis , Adulto , Northern Blotting , Índice de Masa Corporal , Peso Corporal , Femenino , Humanos , Premenopausia , Receptores Tipo I de Factores de Necrosis Tumoral , Receptores Tipo II del Factor de Necrosis Tumoral , Valores de Referencia , Análisis de RegresiónRESUMEN
Recent studies have shown that genetic deficiency of the adipocyte fatty acid-binding protein (aP2) results in minor alterations of plasma lipids and adipocyte development but provides significant protection from dietary obesity-induced hyperinsulinemia and insulin resistance. To identify potential mechanisms responsible for this phenotype, we examined lipolysis and insulin secretion in aP2-/- mice. Beta-adrenergic stimulation resulted in a blunted rise of blood glycerol levels in aP2-/- compared with aP2+/+ mice, suggesting diminished lipolysis in aP2-/- adipocytes. Confirming this, primary adipocytes isolated from aP2-/- mice showed attenuated glycerol and free fatty acid (FFA) release in response to dibutyryl cAMP. The decreased lipolytic response seen in the aP2-/- mice was not associated with altered expression levels of hormone-sensitive lipase or perilipin. The acute insulin secretory response to beta-adrenergic stimulation was also profoundly suppressed in aP2-/- mice despite comparable total concentrations and only minor changes in the composition of systemic FFAs. To address whether levels of specific fatty acids are different in aP2-/- mice, the plasma FFA profile after beta-adrenergic stimulation was determined. Significant reduction in both stearic and cis-11-eicoseneic acids and an increase in palmitoleic acid were observed. The response of aP2-/- mice to other insulin secretagogues such as arginine and glyburide was similar to that of aP2+/+ mice, arguing against generally impaired function of pancreatic beta-cells. Finally, no aP2 expression was detected in isolated pancreatic islet cells. These results provide support for the existence of an adipo-pancreatic axis, the proper action of which relies on the presence of aP2. Consequently, aP2's role in the pathogenesis of type 2 diabetes might involve regulation of both hyperinsulinemia and insulin resistance through its impact on both lipolysis and insulin secretion.
Asunto(s)
Proteínas Portadoras/fisiología , Ácidos Grasos/metabolismo , Insulina/metabolismo , Lipólisis , Proteína P2 de Mielina/fisiología , Proteínas de Neoplasias , Proteínas del Tejido Nervioso , Adipocitos/metabolismo , Tejido Adiposo/metabolismo , Animales , Proteínas Portadoras/metabolismo , Células Cultivadas , Proteína de Unión a los Ácidos Grasos 7 , Proteínas de Unión a Ácidos Grasos , Regulación de la Expresión Génica , Secreción de Insulina , Ratones , Ratones Endogámicos C57BL , Proteína P2 de Mielina/metabolismo , Receptores Adrenérgicos beta/metabolismo , Receptores Adrenérgicos beta 3RESUMEN
Although obesity has become the most common metabolic disorder in the developed world and is highly associated with insulin resistance and noninsulin-dependent diabetes mellitus, the molecular mechanisms underlying these disorders are not clearly understood. Tumor necrosis factor-alpha (TNF-alpha) is overexpressed in obesity and is a candidate mediator of obesity-induced insulin resistance. Complete lack of TNF-alpha function through targeted mutations in TNF-alpha gene or both of its receptors results in significant improvement of insulin sensitivity in dietary, chemical, or genetic models of rodent obesity. In this study, we have analyzed the in vivo role of TNF signaling from p55 [TNF receptor (TNFR) 1] and p75 (TNFR 2) TNFR in the development of insulin resistance by generating genetically obese mice (ob/ob) lacking p55 or p75 TNFRs. In the ob/ob mice, the absence of p55 caused a significant improvement in insulin sensitivity. p75 deficiency alone did not affect insulin sensitivity but might potentiate the effects of p55 deficiency in animals lacking both TNFRs. These results indicate that TNF-alpha is a component of insulin resistance in the ob/ob model of murine obesity and p55 TNFR is the predominant receptor mediating its actions.
Asunto(s)
Resistencia a la Insulina/fisiología , Obesidad/genética , Receptores del Factor de Necrosis Tumoral/metabolismo , Factor de Necrosis Tumoral alfa/fisiología , Animales , Composición Corporal/fisiología , Glucosa/metabolismo , Prueba de Tolerancia a la Glucosa , Homeostasis/fisiología , Lípidos/sangre , Ratones/genética , Ratones/crecimiento & desarrollo , Mutación/fisiología , Obesidad/metabolismo , Obesidad/patología , Receptores del Factor de Necrosis Tumoral/deficiencia , Receptores del Factor de Necrosis Tumoral/genéticaRESUMEN
Adipocyte fatty acid-binding protein, aP2, is a member of the intracellular fatty acid binding protein family. Previously, studies have shown increased insulin sensitivity in aP2-deficient mice with dietary obesity. Here, we asked whether aP2-related alterations in lipolytic response and insulin production are features of obesity-induced insulin resistance and investigated the effects of aP2-deficiency on glucose homeostasis and lipid metabolism in ob/ob mice, a model of extreme obesity. ob/ob mice homozygous for the aP2 null allele (ob/ ob-aP2-/-) became more obese than ob/ob mice as indicated by significantly increased body weight and fat pad size but unaltered body length. However, despite their extreme adiposity, ob/ob-aP2-/- animals were more insulin-sensitive compared with ob/ob controls, as demonstrated by significantly lower plasma glucose and insulin levels and better performance in both insulin and glucose tolerance tests. These animals also showed improvements in dyslipidemia and had lower plasma triglyceride and cholesterol levels. Lipolytic response to beta-adrenergic stimulation and lipolysis-associated insulin secretion was significantly reduced in ob/ob-aP2-/- mice. Interestingly, glucose-stimulated insulin secretion, while virtually abolished in ob/ob controls, was significantly improved in ob/ob-aP2-/- animals. There were no apparent morphological differences in the structure or size of the pancreatic islets between genotypes. Taken together, the data indicate that in obesity, aP2-deficiency not only improves peripheral insulin resistance but also preserves pancreatic beta cell function and has beneficial effects on lipid metabolism.
Asunto(s)
Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Glucosa/metabolismo , Metabolismo de los Lípidos , Proteína P2 de Mielina/genética , Proteína P2 de Mielina/metabolismo , Proteínas de Neoplasias , Proteínas del Tejido Nervioso , Obesidad/metabolismo , Tejido Adiposo/fisiología , Envejecimiento/fisiología , Animales , Peso Corporal/fisiología , Proteína de Unión a los Ácidos Grasos 7 , Proteínas de Unión a Ácidos Grasos , Ácidos Grasos/metabolismo , Femenino , Homeostasis/fisiología , Insulina/metabolismo , Islotes Pancreáticos/metabolismo , Leptina/metabolismo , Lipólisis/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Obesos , Obesidad/genética , Páncreas/metabolismo , Caracteres SexualesRESUMEN
As obesity is a major risk factor for noninsulin-dependent diabetes mellitus, adipose tissue may generate a mediator that influences the activity of insulin on various target tissues. Recent evidence suggests that a cytokine, tumor necrosis factor-alpha (TNF alpha), may serve this role. This study investigates whether the expression of TNF alpha and its receptors is modulated during drug treatment to reduce insulin resistance. The effects of moderate weight loss by dietary restriction were also examined. We show here that a marked induction of TNF alpha mRNA occurs in adipose tissues from a mouse model of obesity-linked diabetes (KKAy) compared to that in nondiabetic mice (C57). Likewise, RNA transcripts encoding TNF R2 receptors (p75) were significantly increased in fat tissues of the obese diabetic animals. In muscle from these diabetic animals, RNA transcripts encoding both TNF R1 (p55) and R2 were significantly elevated, although R2 transcript abundance was less elevated than in fat. We also observed that the overexpression of mRNA for TNF alpha and both of its receptors could be at least partly normalized by treatment of the diabetic animals with the insulin-sensitizing agent pioglitazone. Treating of the obese diabetic animals by food restriction reduced the expression of mRNA for TNF R2 in muscle, but not fat. These results clearly indicate that gene expression for the TNF systems can be regulated by an insulin-sensitizing drug and reduction of body weight. Such findings support a role for this cytokine in the insulin-resistant diabetic state and show its modulation by therapies that reverse the disorder.
Asunto(s)
Diabetes Mellitus/dietoterapia , Diabetes Mellitus/genética , Expresión Génica , Resistencia a la Insulina , Obesidad , Receptores del Factor de Necrosis Tumoral/genética , Tiazolidinedionas , Factor de Necrosis Tumoral alfa/genética , Animales , Diabetes Mellitus/tratamiento farmacológico , Diabetes Mellitus Tipo 2 , Femenino , Hipoglucemiantes/uso terapéutico , Masculino , Ratones , Ratones Endogámicos C57BL , Músculos/metabolismo , Pioglitazona , ARN Mensajero/metabolismo , Tiazoles/uso terapéuticoRESUMEN
UNLABELLED: Circulating IL-6 levels are elevated in obesity. Although IL-6 is expressed in adipose tissue, neither its regulation nor cell of origin is well characterized. Here we investigated the beta-adrenergic regulation of IL-6 release in a combination of studies on humans and animals in vivo and cultured adipocytes in vitro. Human in vivo study: Human volunteers were infused with isoproterenol, norepinephrine, or saline [4 M:4F; mean (SD) age 35.5 (5.8) yr; body mass index 24.6 (4.2) kg/m(-2)]. Plasma IL-6 levels increased during a 3-h infusion of isoproterenol (P = 0.01) and fell 2 h post infusion (P = 0.05). IL-6 levels did not change significantly with either norepinephrine or saline. Murine in vivo study: C57BL6/J male mice were injected ip with dobutamine (beta(1) agonist), clenbuterol (beta(2)), CL316243 (beta(3)), or saline placebo. Plasma IL-6 levels at 3 h were increased by clenbuterol (P = 0.02) and CL316243 (P = 0.02) but not dobutamine (P = 0.51), compared with placebo. IN VITRO STUDIES: In human peripheral blood cells, lipopolysaccharide treatment enhanced secretion of IL-6 (vs. controls; P < 0.001), whereas isoproterenol inhibited IL-6 secretion (P = 0.012) and norepinephrine had no significant effect. In contrast, isolated human adipocytes and differentiated 3T3F442A adipocytes all rapidly secreted IL-6 in response to adrenergic agonists (P < 0.01, compared with untreated cells). We conclude that beta 2/beta 3 adrenoceptor stimulation on adipocytes, rather than macrophages, may be responsible for the increases in plasma IL-6 concentrations observed during sympathetic activation and in obesity.
Asunto(s)
Tejido Adiposo/metabolismo , Interleucina-6/metabolismo , Receptores Adrenérgicos beta/fisiología , Adipocitos/efectos de los fármacos , Adipocitos/metabolismo , Agonistas alfa-Adrenérgicos/farmacología , Agonistas Adrenérgicos beta/farmacología , Adulto , Animales , Clenbuterol/farmacología , Dioxoles/farmacología , Dobutamina/farmacología , Femenino , Humanos , Técnicas In Vitro , Interleucina-6/antagonistas & inhibidores , Interleucina-6/sangre , Isoproterenol/farmacología , Masculino , Ratones , Ratones Endogámicos C57BL , Norepinefrina/farmacologíaRESUMEN
Tumor necrosis factor-alpha (TNFalpha) has profound effects on cultured adipocytes, one of which is the inhibition of terminal differentiation. Previous studies in TNF receptor (TNFR)-deficient preadipocytes have demonstrated that the anti-adipogenic effect of both secreted and transmembrane TNFalpha is mediated solely by TNFR1. In this study, we performed a structure-function analysis of the intracellular domains of TNFR1 and investigated the signaling pathway(s) involved in TNFR1-mediated inhibition of adipocyte differentiation. Our results show that repression of adipogenesis required the juxtamembrane and death domains and was independent of the pathways involving nuclear factor kappaB and neutral sphingomyelinase.
Asunto(s)
Adipocitos/fisiología , Antígenos CD/metabolismo , Diferenciación Celular/fisiología , Receptores del Factor de Necrosis Tumoral/metabolismo , Transducción de Señal/fisiología , Adipocitos/citología , Adipocitos/efectos de los fármacos , Animales , Antígenos CD/genética , Biomarcadores , Línea Celular , Genes Reporteros/genética , Mutagénesis Sitio-Dirigida , FN-kappa B/genética , FN-kappa B/metabolismo , Estructura Terciaria de Proteína , Receptores del Factor de Necrosis Tumoral/genética , Receptores Tipo I de Factores de Necrosis Tumoral , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Relación Estructura-Actividad , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Tumour necrosis factor-alpha (TNFalpha) is a multifunctional cytokine that exerts a myriad of biological actions in numerous different tissues including adipocytes through its two distinct cell surface receptors. To address the role of each TNF receptor in the biological actions of TNFalpha in adipocytes, we have developed four new preadipocyte cell lines. These were established from wild type controls (TNFR1(+/+)R2(+/+)) and from mice lacking TNFR1 (TNFR1(-/-)), TNFR2 (TNFR2(-/-)) or both (TNFR1(-/-)R2(-/-)). All four new cell lines can fully differentiate to form mature adipocytes, under appropriate culture conditions, as judged by cell morphology, expression of multiple adipogenic markers and the ability to mediate agonist-stimulated lipolysis and insulin-stimulated glucose transport. In wild type (TNFR1(+/+)R2(+/+)) and TNFR2(-/-) adipocytes, TNFalpha stimulated lipolysis and inhibited insulin-stimulated glucose transport as well as insulin receptor autophosphorylation. In contrast, these activities were completely lost in the TNFR1(-/-)R2(-/-) and TNFR1(-/-) cells. Taken together, these studies demonstrate that TNFalpha-induced lipolysis, as well as inhibition of insulin-stimulated glucose transport are predominantly mediated by TNFR1 and that the presence of TNFR2 is not necessary for these functions. This new experimental system promises to be useful in dissecting the molecular pathways activated by each TNF receptor in mediating the biological functions of TNFalpha in differentiated adipocytes.
Asunto(s)
Adipocitos/metabolismo , Receptores del Factor de Necrosis Tumoral/deficiencia , Factor de Necrosis Tumoral alfa/metabolismo , Adipocitos/efectos de los fármacos , Animales , Diferenciación Celular , Línea Celular , Regulación de la Expresión Génica , Glucosa/metabolismo , Insulina/farmacología , Lipólisis/efectos de los fármacos , Ratones , Ratones Noqueados , Fosforilación , Receptor de Insulina/metabolismoRESUMEN
Obesity is related to cardiovascular disease (CVD) morbidity and mortality, however, the mechanisms for the development of obesity-induced CVD risk remain unclear. Hyperinsulinemia and insulin resistance are considered key components in the metabolic cardiovascular syndrome and as independent risk factors for CVD. Plasma leptin and tumor necrosis factor-alpha (TNF-alpha), two adipocyte products, are also proposed to be associated with the development of CVD risk. The purpose of this study is to evaluate the association of plasma leptin, soluble TNF receptors (sTNF-R), and insulin levels as possible mediators of the effect of obesity on atherogenic and thrombogenic CVD risk factors among men. From the Health Professionals Follow-up Study (HPFS), we selected 268 men, aged 47--83 years, who were free of CVD, diabetes, and cancer (except non-melanoma skin cancer), and who had provided a fasting blood sample in 1994. We measured plasma insulin and leptin levels by radioimmunoassay and sTNF-R levels by ELISA. Men in the highest quintile of body mass index (BMI, mean=30.5 kg/m(2)) were less physically active and had a more adverse cardiovascular lipid and homeostatic profile, as indicated by levels of insulin, triglyceride (TG), tissue plasminogen activator (t-PA) antigen levels, and apolipoprotein A1 (Apo-A1). In a multivariate regression model controlling for age, smoking, alcohol intake, physical activity and diet, BMI was inversely associated with HDL-cholesterol (HDL-C) and Apo-A1 and positively associated with TG, Apo-B and t-PA antigen levels. The associations between BMI and these CVD risk factors were only slightly changed after adjusting for leptin and/or sTNF-R; but were substantially attenuated after controlling for insulin levels. These data suggest that the association between obesity and biological predictors of CVD may be mediated through changes in plasma insulin, rather than leptin or sTNF-R levels. However, plasma leptin may still play a role in CVD through independent effects on lipid metabolism.