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1.
Anal Chem ; 95(13): 5507-5513, 2023 04 04.
Artículo en Inglés | MEDLINE | ID: mdl-36961992

RESUMEN

Quartz-crystal-microbalance (QCM) biosensor is a typical label-free biosensor, and its sensitivity can be greatly improved by removing electrodes and wires that would be otherwise attached to the surfaces of the quartz resonator. The wireless-electrodeless QCM biosensor was then developed using a microelectro-mechanical systems (MEMS) process, although challenges remain in the sensitivity, the coupling efficiency, and the miniaturization (or mass production). In this study, we establish a MEMS process to obtain a large number of identical ultrasensitive and highly efficient sensor chips with dimensions of 6 mm square. The fundamental shear resonance frequency of the thinned AT-cut quartz resonator packaged in the microchannel exceeds 160 MHz, which is excited by antennas deposited on inner walls of the microchannel, significantly improving the electro-mechanical coupling efficiency in the wireless operation. The high sensitivity of the developed MEMS QCM biosensors is confirmed by the immunoglobulin G (IgG) detection using protein A and ZZ-tag displaying a bionanocapsule (ZZ-BNC), where we find that the ZZ-BNC can provide more effective binding sites and higher affinity to the target molecules, indicating a further enhancement in the sensitivity of the MEMS QCM biosensor. We then perform the label-free C-reactive protein (CRP) detection using the ZZ-BNC-functionalized MEMS QCM biosensor, which achieves a detection limit of 1 ng mL-1 or less even with direct detection.


Asunto(s)
Técnicas Biosensibles , Sistemas Microelectromecánicos , Cuarzo/química , Proteína C-Reactiva , Miniaturización , Técnicas Biosensibles/métodos , Tecnicas de Microbalanza del Cristal de Cuarzo/métodos
2.
Biosci Biotechnol Biochem ; 87(7): 765-770, 2023 Jun 23.
Artículo en Inglés | MEDLINE | ID: mdl-37096394

RESUMEN

The detection sensitivity of immunostick colorimetric assay has been increased by using a bio-nanocapsule as a scaffold for oriented immobilization of immunoglobulin Gs. This immunostick produced ∼82-folds stronger coloration in the detection of food allergens and reduced detection time by a factor of 5.


Asunto(s)
Hipersensibilidad a los Alimentos , Nanocápsulas , Humanos , Colorimetría , Inmunoglobulina G , Hipersensibilidad a los Alimentos/diagnóstico , Alérgenos
3.
Analyst ; 147(3): 489-495, 2022 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-35023508

RESUMEN

The oriented immobilization of sensing molecules (e.g., IgGs, receptors, lectins, and DNA aptamers) on sensor chips is particularly important for maximizing the potential of the sensing molecules, thereby enhancing the sensitivity and target-binding capacity of biosensors. We previously developed ∼30 nm bio-nanocapsules (ZZ-BNCs) consisting of the hepatitis B virus envelope L protein fused with the tandem form of protein A-derived IgG Fc-binding Z domain (ZZ-L protein). ZZ-BNC acts successfully as a scaffold, enhancing both the sensitivity and binding capacity of IgG, a Fc-fused receptor, and Fc-fused lectin to antigens, cytokines, and sugar chains through an oriented immobilization on a biosensor surface. To expand the versatility of ZZ-BNC, we modified ZZ-BNC by replacing the ZZ domain with a DNA-binding single-chain lambda Cro (scCro) domain, thereby developing scCro-BNC. The scCro-BNC was synthesized in yeast cells and homogeneously purified as ∼30 nm sized nanoparticles. In a quartz crystal microbalance, an scCro-BNC-coated sensor chip immobilized with thrombin-binding DNA aptamers showed an ∼5.5-fold higher thrombin-binding capacity and ∼6000-fold higher detection sensitivity than a sensor chip directly coated with DNA aptamers. In addition, the number of bound thrombin molecules per molecule of DNA aptamer increased by ∼7.8-fold with an scCro-BNC coating, consistent with the theoretical thrombin-binding capacity. Collectively, scCro-BNC was shown to perform as an ideal scaffold for maximizing the potential of the DNA aptamer by immobilizing it in an oriented manner. Facilitating a highly sensitive detection of various target molecules, these BNC-based scaffolds are expected to improve a wide range of biosensors while minimizing the number of sensing molecules required.


Asunto(s)
Aptámeros de Nucleótidos , Técnicas Biosensibles , Nanocápsulas , Inmunoglobulina G , Proteína Estafilocócica A
4.
Biosci Biotechnol Biochem ; 86(12): 1658-1669, 2022 Nov 23.
Artículo en Inglés | MEDLINE | ID: mdl-36243901

RESUMEN

Black tea extracts (BTEs) from four different production areas showed a higher aggregation strength for phosphatidylcholine-based liposomes containing cholesterol used as a viral membrane model. Furthermore, the anti-influenza A virus (IAV) activity of each BTE in vitro demonstrated that although Sri Lanka, Kenya, and Assam had higher anti-IAV activities, Darjeeling had a lower anti-IAV activity, showing a correlation between each BTE and the liposome aggregation strength. Moreover, the antiviral activity strength of BTEs was consistent with the antioxidant activity strength of BTEs, suggesting that the component(s) in black tea that exhibits antioxidant activity would also be the component(s) that accounts for its antiviral activity. Thus, our results propose that BTEs exert their antiviral effects by binding not only hemagglutinin and neuraminidase but also viral membranes directly, especially "cholesterol-rich lipid rafts" and affect the membrane structure, causing the virus to aggregate, thereby inhibiting infection of the host cells.


Asunto(s)
Antivirales , Camellia sinensis , Antivirales/farmacología , , Liposomas , Antioxidantes , Colesterol , Replicación Viral
5.
Biosci Biotechnol Biochem ; 84(9): 1775-1779, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32475227

RESUMEN

We report a novel scaffold for clustering and oriented immobilization of human IgG1 Fc-fused lectins on biosensors without chemical modifications. This approach uses a bio-nanocapsule (BNC) displaying a tandem form of IgG Fc-binding Z domains derived from Staphylococcus aureus protein A (ZZ-BNC). Incorporating ZZ-BNC effectively increased both the sensitivity and sugar chain-binding capacity compared with the condition without ZZ-BNC.


Asunto(s)
Técnicas Biosensibles/métodos , Proteínas Inmovilizadas/metabolismo , Fragmentos Fc de Inmunoglobulinas/genética , Lectinas/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Azúcares/análisis , Humanos , Proteínas Inmovilizadas/química , Inmunoglobulina G/inmunología , Lectinas/química , Dominios Proteicos , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteína Estafilocócica A/química , Azúcares/química
6.
Sensors (Basel) ; 20(19)2020 Oct 08.
Artículo en Inglés | MEDLINE | ID: mdl-33050090

RESUMEN

The influence of nivolumab on intercellular adhesion forces between T cells and cancer cells was evaluated quantitatively using atomic force microscopy (AFM). Two model T cells, one expressing high levels of programmed cell death protein 1 (PD-1) (PD-1high Jurkat) and the other with low PD-1 expression levels (PD-1low Jurkat), were analyzed. In addition, two model cancer cells, one expressing programmed death-ligand 1 (PD-L1) on the cell surface (PC-9, PD-L1+) and the other without PD-L1 (MCF-7, PD-L1-), were also used. A T cell was attached to the apex of the AFM cantilever using a cup-attached AFM chip, and the intercellular adhesion forces were measured. Although PD-1high T cells adhered strongly to PD-L1+ cancer cells, the adhesion force was smaller than that with PD-L1- cancer cells. After the treatment of PD-1high T cells with nivolumab, the adhesion force with PD-L1+ cancer cells increased to a similar level as with PD-L1- cancer cells. These results can be explained by nivolumab influencing the upregulation of the adhesion ability of PD-1high T cells with PD-L1+ cancer cells. These results were obtained by measuring intercellular adhesion forces quantitatively, indicating the usefulness of single-cell AFM analysis.


Asunto(s)
Adhesión Celular/efectos de los fármacos , Microscopía de Fuerza Atómica , Nivolumab/farmacología , Linfocitos T/citología , Antígeno B7-H1 , Humanos , Células Jurkat , Células MCF-7 , Receptor de Muerte Celular Programada 1 , Análisis Espectral
7.
Int J Mol Sci ; 21(15)2020 Aug 02.
Artículo en Inglés | MEDLINE | ID: mdl-32748844

RESUMEN

Ongoing aortic wall degeneration and subsequent aneurysm exclusion failure are major concerns after an endovascular aneurysm repair with a stent-graft. An ideal solution would be a drug therapy that targets the aortic wall and inhibits wall degeneration. Here, we described a novel drug delivery system, which allowed repetitively charging a graft with therapeutic drugs and releasing them to the aortic wall in vivo. The system was composed of a targeted graft, which was labeled with a small target molecule, and the target-recognizing nanocarrier, which contained suitable drugs. We developed the targeted graft by decorating a biotinylated polyester graft with neutravidin. We created the target-recognizing nanocarrier by conjugating drug-containing liposomes with biotinylated bio-nanocapsules. We successfully demonstrated that the target-recognizing nanocarriers could bind to the targeted graft, both in vitro and in blood vessels of live mice. Moreover, the drug released from our drug delivery system reduced the expression of matrix metalloproteinase-9 in mouse aortas. Thus, this hybrid system represents a first step toward an adjuvant therapy that might improve the long-term outcome of endovascular aneurysm repair.


Asunto(s)
Aorta/efectos de los fármacos , Aneurisma de la Aorta/terapia , Prótesis Vascular , Sistemas de Liberación de Medicamentos/métodos , Metaloproteinasa 9 de la Matriz/metabolismo , Quinolinas/administración & dosificación , Animales , Aorta/metabolismo , Aorta/patología , Avidina/química , Portadores de Fármacos/química , Inhibidores de Hidroximetilglutaril-CoA Reductasas/administración & dosificación , Inhibidores de Hidroximetilglutaril-CoA Reductasas/química , Masculino , Ratones Endogámicos C57BL , Microscopía Fluorescente , Nanoestructuras/química , Diseño de Prótesis , Quinolinas/química , Resultado del Tratamiento
8.
J Gene Med ; 21(12): e3140, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31697013

RESUMEN

BACKGROUND: The uterus is an organ that is directly accessible via the transvaginal route, whereas the drug delivery system and the gene delivery system (GDS) for the uterus are very limited, even in animal models. In the present study, we optimized a bionanocapsule (BNC) comprising a hepatitis B virus envelope L-protein particle, for which a structurally similar particle has been used as an immunogen of a conventional HB vaccine worldwide for more than 30 years, as a local uterine GDS using a mouse model. METHODS: To display various antibodies for re-targeting to different cells other than hepatic cells, the pre-S1 region of BNC was replaced with a tandem form of the protein A-derived immunoglobulin G Fc-interacting region (Z domain, ZZ-BNC). To induce strong cell adhesion after local administration into the uterine cavity, ZZ-BNC was modified with a transactivator of transcription (TAT) peptide. RESULTS: Gene transfer using TAT-modified ZZ-BNC is approximately 5000- or 18-fold more efficient than the introduction of the same dose of naked DNAs or the use of the cationic liposomes, respectively. TAT-modified ZZ-BNC was rapidly eliminated from the uterus and had no effect on the pregnancy rate, litter size or fetal growth. CONCLUSIONS: TAT-modified ZZ-BNC could be a useful GDS for uterine endometrial therapy via local uterine injection.


Asunto(s)
Técnicas de Transferencia de Gen , Nanopartículas , Péptidos , Útero/metabolismo , Productos del Gen tat del Virus de la Inmunodeficiencia Humana , Animales , Femenino , Expresión Génica , Genes Reporteros , Inmunohistoquímica , Ratones , Nanopartículas/química , Péptidos/química , Embarazo , Transgenes , Proteínas del Envoltorio Viral/química , Productos del Gen tat del Virus de la Inmunodeficiencia Humana/química
9.
J Nanobiotechnology ; 16(1): 59, 2018 Aug 04.
Artículo en Inglés | MEDLINE | ID: mdl-30077180

RESUMEN

BACKGROUND: Various nanocarriers have been used to deliver subunit vaccines specifically to dendritic cells (DCs) for the improvement of immunogenicity. However, due to their insufficient DC priming ability, these vaccines could not elicit effective innate immunity. We have recently developed a DC-targeting bio-nanocapsule (BNC) by displaying anti-CD11c IgGs via protein A-derived IgG Fc-binding Z domain on the hepatitis B virus envelope L protein particles (α-DC-ZZ-BNC). RESULTS: After the chemical modification with antigens (Ags), the α-DC-ZZ-BNC-Ag complex could deliver Ags to DCs efficiently, leading to effective DC maturation and efficient endosomal escape of Ags, followed by Ag-specific T cell responses and IgG productions. Moreover, the α-DC-ZZ-BNC modified with Japanese encephalitis virus (JEV) envelope-derived D3 Ags could confer protection against 50-fold lethal dose of JEV injection on mice. CONCLUSION: The α-DC-ZZ-BNC-Ag platform was shown to induce humoral and cellular immunities effectively without any adjuvant.


Asunto(s)
Antígeno CD11c/inmunología , Células Dendríticas/inmunología , Inmunogenicidad Vacunal , Vacunas contra la Encefalitis Japonesa/inmunología , Nanocápsulas/química , Animales , Antígenos Virales/administración & dosificación , Antígenos Virales/inmunología , Línea Celular , Células Dendríticas/metabolismo , Virus de la Encefalitis Japonesa (Especie)/química , Virus de la Encefalitis Japonesa (Especie)/fisiología , Humanos , Inmunidad Celular , Inmunoglobulina G/biosíntesis , Inmunoglobulina G/inmunología , Vacunas contra la Encefalitis Japonesa/administración & dosificación , Ratones Endogámicos BALB C , Ovalbúmina/química , Tamaño de la Partícula , Proteína Estafilocócica A/química , Proteínas del Envoltorio Viral/química
10.
Nano Lett ; 17(11): 7117-7124, 2017 11 08.
Artículo en Inglés | MEDLINE | ID: mdl-29047282

RESUMEN

Focusing on intracellular targets, we propose a new cell separation technique based on a nanoneedle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift ("fish") individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nanoneedles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nanoneedles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.


Asunto(s)
Anticuerpos Inmovilizados/química , Separación Celular/instrumentación , Nanoestructuras/química , Agujas , Animales , Línea Celular , Diseño de Equipo , Células HeLa , Humanos , Células Madre Pluripotentes Inducidas/citología , Células MCF-7 , Ratones , Células 3T3 NIH , Nanoestructuras/ultraestructura , Células-Madre Neurales/citología , Análisis de Matrices Tisulares/instrumentación
11.
Biochem Biophys Res Commun ; 490(2): 155-160, 2017 08 19.
Artículo en Inglés | MEDLINE | ID: mdl-28601634

RESUMEN

Hepatitis B virus (HBV) envelope particles have been synthesized in eukaryotic cells (e.g., mammalian cells, insect cells, and yeast cells) as an HB vaccine immunogen and drug delivery system (DDS) nanocarrier. Many researchers had made attempts to synthesize the particles in Escherichia coli for minimize the cost and time for producing HBV envelope particles, but the protein was too deleterious to be synthesized in E. coli. In this study, we generated deletion mutants of HBV envelope L protein (389 amino acid residues (aa)) containing three transmembrane domains (TM1, TM2, TM3). The ΔNC mutant spanning from TM2 to N-terminal half of TM3 (from 237 aa to 335 aa) was found as a shortest form showing spontaneous particle formation. After the N-terminal end of ΔNC mutant was optimized by the N-end rule for E. coli expression, the modified ΔNC mutant (mΔNC) was efficiently expressed as particles in E. coli. The molecular mass of mΔNC particle was approx. 670 kDa, and the diameter was 28.5 ± 6.2 nm (mean ± SD, N = 61). The particle could react with anti-HBV envelope S protein antibody, indicating the particles exhibited S antigenic domain outside as well as HBV envelope particles. Taken together, the E. coli-derived mΔNC particles could be used as a substitute of eukaryotic cell-derived HBV envelope particles for versatile applications.


Asunto(s)
Escherichia coli/metabolismo , Proteínas del Envoltorio Viral/biosíntesis , Animales , Células COS , Chlorocebus aethiops , Mutación , Tamaño de la Partícula , Proteínas del Envoltorio Viral/química , Proteínas del Envoltorio Viral/metabolismo
12.
Glycobiology ; 26(11): 1180-1189, 2016 11.
Artículo en Inglés | MEDLINE | ID: mdl-27329181

RESUMEN

The functions of cell surface proteins, such as growth factor receptors and virus/bacteria-entry receptors, can be dynamically regulated by oligosaccharide modifications. In the present study, we investigated the involvement of glycosylation in hepatitis B virus (HBV) entry into hepatoma cells. Infection of oligosaccharide-remodeling hepatoma cells with a pseudo virus of HBV, bio-nanocapsule (BNC), was evaluated by flow cytometry and confocal microscopy. Among various experiments using several hepatoma cells, marked difference was observed between Huh6 cells and HB611 cells, which were established by HBV gene transfection into hepatoma cells. Comprehensive oligosaccharide analysis showed dramatic increases of core fucosylation in HB611 cells, compared with Huh6 cells. Knock down of fucosyltransferase 8 (FUT8) reduced BNC entry into HB611 cells. In contrast, overexpression of FUT8 in Huh6 cells increased BNC entry. Although expression of sodium taurocholate cotransporting polypeptide (NTCP), which is one of HBV receptors was very similar between Huh6 and HB611 cells, proteins coprecipitated with NTCP were dependent on levels of core-fucosylation, suggesting that core-fucosylation regulates BNC entry into hepatoma cells. Our findings demonstrate that core-fucosylation is an important glycosylation for HBV infection of hepatoma cells through HBV-receptor-mediated endocytosis. Down-regulation of core-fucosylation may be a novel target for HBV therapy.


Asunto(s)
Fucosa/metabolismo , Virus de la Hepatitis B/metabolismo , Hepatitis B/metabolismo , Glicosilación , Virus de la Hepatitis B/genética , Humanos , Nanocápsulas/química , Células Tumorales Cultivadas
13.
Biochem Biophys Res Commun ; 474(2): 413-420, 2016 05 27.
Artículo en Inglés | MEDLINE | ID: mdl-27114303

RESUMEN

Enigma Homolog 1 (ENH1) is a scaffold protein for signaling proteins and transcription factors. Previously, we reported that ENH1 overexpression promotes the differentiation of C2C12 myoblasts. However, the molecular mechanism underlying the role of ENH1 in the C2C12 cells differentiation remains elusive. ENH1 was shown to inhibit the proliferation of neuroblastoma cells by sequestering Inhibitor of DNA binding protein 2 (Id2) in the cytosol. Id2 is a repressor of basic Helix-Loop-Helix transcription factors activity and prevents myogenesis. Here, we found that ENH1 overcome the Id2 repression of C2C12 cells myogenic differentiation and that ENH1 overexpression promotes mice satellite cells activation, the first step toward myogenic differentiation. In addition, we show that ENH1 interacted with Id2 in C2C12 cells and mice satellite cells. Collectively, our results suggest that ENH1 plays an important role in the activation of myogenesis through the repression of Id2 activity.


Asunto(s)
Proteínas Adaptadoras Transductoras de Señales/metabolismo , Proteína 1 Inhibidora de la Diferenciación/metabolismo , Proteínas de Microfilamentos/metabolismo , Desarrollo de Músculos/fisiología , Mioblastos/citología , Mioblastos/metabolismo , Proteínas Asociadas a Matriz Nuclear/metabolismo , Animales , Diferenciación Celular/fisiología , Línea Celular , Ratones
14.
Biochem Biophys Res Commun ; 474(2): 406-412, 2016 05 27.
Artículo en Inglés | MEDLINE | ID: mdl-27120459

RESUMEN

A hollow nanoparticle known as a bio-nanocapsule (BNC) consisting of hepatitis B virus (HBV) envelope L protein and liposome (LP) can encapsulate drugs and genes and thereby deliver them in vitro and in vivo to human hepatic tissues, specifically by utilizing the HBV-derived infection machinery. Recently, we identified a low pH-dependent fusogenic domain at the N-terminal part of the pre-S1 region of the HBV L protein (amino acid residues 9 to 24; NPLGFFPDHQLDPAFG), which shows membrane destabilizing activity (i.e., membrane fusion, membrane disruption, and payload release) upon interaction with target LPs. In this study, instead of BNC and HBV, we generated LPs displaying a mutated form of the pre-S1 (9-24) peptide, and performed a membrane disruption assay using target LPs containing pyranine (fluorophore) and p-xylene-bis (N-pyridinium bromide) (DPX) as a quencher. The membrane disruption activity was found to correlate with the hydrophobicity of the whole structure, while the peptide retained a random-coil structure even under low pH condition. One large hydrophobic cluster (I) and one small hydrophobic cluster (II) residing in the peptide would be connected by the protonation of residues D16 and D20, and thereby exhibit strong membrane disruption activity in a low pH-dependent manner. Furthermore, the introduction of a positively charged residue enhanced the activity significantly, suggesting that a sole positively charged residue (H17) may be important for the interaction with target LPs by electrostatic interaction. Collectively, these results suggest that the pre-S1 (9-24) peptide may be involved in the endosomal escape of the BNC's payloads, as well as in the HBV uncoating process.


Asunto(s)
Membrana Celular/química , Análisis Mutacional de ADN/métodos , Antígenos de Superficie de la Hepatitis B/química , Antígenos de Superficie de la Hepatitis B/genética , Virus de la Hepatitis B/genética , Mutación/genética , Precursores de Proteínas/química , Precursores de Proteínas/genética , Secuencia de Bases , Concentración de Iones de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Datos de Secuencia Molecular , Polimorfismo de Nucleótido Simple/genética , Dominios Proteicos/genética
15.
Biotechnol Bioeng ; 113(8): 1796-804, 2016 08.
Artículo en Inglés | MEDLINE | ID: mdl-26853220

RESUMEN

Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved in proliferation, differentiation, apoptosis, and oncogenic transformation. Hence, it is important for drug discovery and system biology to measure the intracellular level of phosphotyrosine. Although mammalian cells have been conventionally utilized for this purpose, accurate determination of phosphotyrosine level often suffers from high background due to the unexpected crosstalk among endogenous signaling molecules. This situation led us firstly to establish the ligand-induced activation of homomeric receptor tyrosine kinase (i.e., epidermal growth factor receptor) in Saccharomyces cerevisiae, a lower eukaryote possessing organelles similar to higher eukaryote but not showing substantial level of tyrosine kinase activity. In this study, we expressed heteromeric receptor tyrosine kinase (i.e., a complex of interleukin-5 receptor (IL5R) α chain, common ß chain, and JAK2 tyrosine kinase) in yeast. When coexpressed with a cell wall-anchored form of IL5, the yeast exerted the autophosphorylation of JAK2, followed by the phosphorylation of transcription factor STAT5a and subsequent nuclear accumulation of phosphorylated STAT5a. Taken together, yeast could be an ideal host for sensitive detection of phosphotyrosine generated by a wide variety of tyrosine kinases. Biotechnol. Bioeng. 2016;113: 1796-1804. © 2016 Wiley Periodicals, Inc.


Asunto(s)
Citocinas/metabolismo , Quinasas Janus/metabolismo , Factores de Transcripción STAT/metabolismo , Saccharomyces cerevisiae/metabolismo , Técnicas de Visualización de Superficie Celular , Fosforilación , Fosfotirosina/metabolismo , Proteínas Tirosina Quinasas/metabolismo , Transducción de Señal
16.
J Biol Chem ; 289(14): 9781-94, 2014 Apr 04.
Artículo en Inglés | MEDLINE | ID: mdl-24563467

RESUMEN

NELL1 is a large oligomeric secretory glycoprotein that functions as an osteoinductive factor. NELL1 contains several conserved domains, has structural similarities to thrombospondin 1, and supports osteoblastic cell adhesion through integrins. To define the structural requirements for NELL1-mediated cell adhesion, we prepared a series of recombinant NELL1 proteins (intact, deleted, and cysteine-mutant) from a mammalian expression system and tested their activities. A deletion analysis demonstrated that the C-terminal cysteine-rich region of NELL1 is critical for the cell adhesion activity of NELL1. Reducing agent treatment decreased the cell adhesion activity of full-length NELL1 but not of its C-terminal fragments, suggesting that the intramolecular disulfide bonds within this region are not functionally necessary but that other disulfide linkages in the N-terminal region of NELL1 may be involved in cell adhesion activity. By replacing cysteine residues with serines around the coiled-coil domain of NELL1, which is responsible for oligomerization, we created a mutant NELL1 protein that was unable to form homo-oligomers, and this monomeric mutant showed substantially lower cell adhesion activity than intact NELL1. These results suggest that an oligomerization-induced conformational change in the C-terminal region of NELL1 is important for the efficient mediation of cell adhesion and spreading by NELL1.


Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Movimiento Celular/fisiología , Glicoproteínas/metabolismo , Multimerización de Proteína/fisiología , Animales , Proteínas de Unión al Calcio/genética , Adhesión Celular/fisiología , Línea Celular , Glicoproteínas/genética , Ratones , Mutación , Estructura Cuaternaria de Proteína , Estructura Terciaria de Proteína
17.
Plant Biotechnol (Tokyo) ; 41(2): 165-168, 2024 Jun 25.
Artículo en Inglés | MEDLINE | ID: mdl-39463775

RESUMEN

Papaya (Carica papaya L.) is a herbaceous plant belonging to the family Caricaceae in the order Brassicales. The shape of papaya fruit was linked to sex, and the fruit of female plants is round, whereas that of hermaphrodites is pyriform. Although fruit shape preferences vary by region, differences in their functionalities have not been investigated. Since unripe fruit, also called green papaya, is known for its nutritional and therapeutic benefits, we performed a metabolome analysis of unripe papaya using liquid chromatography coupled with quadrupole/time of flight mass spectrometry. We first focused on capraine derivatives, major piperidine alkaloids, and bioactive compounds with significant antiplasmodial activity. Interestingly, carpaine derivatives tended to be altered in the peel and pulp but not in the seed. Multivariate analyses indicated little difference or minor differences to the extent that they can be caused by individual differences in metabolite profiling between the two sexes. Conversely, total polyphenol content and proteolytic activity were also investigated, but there were no differences between females and hermaphrodites for total polyphenol content and proteolytic activity. In conclusion, the metabolome and major functionalities were similar between hermaphrodites and female unripe fruit. However, it would be worth considering the sex of the material fruit, especially when focusing on the functional properties of carpaine derivatives.

18.
Anal Chem ; 85(3): 1753-9, 2013 Feb 05.
Artículo en Inglés | MEDLINE | ID: mdl-23297690

RESUMEN

For establishing cells that secrete antibodies most efficiently (e.g., hybridomas, CHO (Chinese hamster ovary) cells), the screening and subsequent breeding of promising cells have been performed at the single-colony level, which requires several weeks to propagate a substantial number of cells by forming colonies from single cells for evaluation by the conventional assays. However, this screening process lacks high-throughput performance in time and colony numbers. Therefore, development of novel methods is expected to identify single cells secreting higher amounts of antibodies in real-time and in a nondestructive manner without colony formation. In this study, we prepared lipid-labeled antimouse IgG Fc antibodies (capture molecules) that were uniformly displayed on the surface of candidate cells. Secreted nascent antibodies were subsequently sandwiched between capture molecules and fluorescence-labeled antimouse IgG F(ab')(2) F(ab')(2) (detection molecules). This newly developed method is hereinafter referred to as a cell surface-fluorescence immunosorbent assay (CS-FIA). The fluorescence intensity of each cell was found to correlate well with the amount of sandwiched antibodies (from 6.25 fg/cell to 6.40 pg/cell). When about 4 × 10(3) cells of mouse hybridomas were subjected to CS-FIA, we isolated 28 hybridomas showing the highest fluorescence intensity within a day. Furthermore, after propagation of single cells to about 10(5) cells (after 2 weeks), 20 hybridomas were still able to secrete higher amounts (up to 7-fold) of antibodies than parental hybridomas. Our results demonstrate that CS-FIA is a powerful method for the single-cell-based establishment of cells that secrete most efficiently not only antibodies but also various biomolecules.


Asunto(s)
Anticuerpos/metabolismo , Membrana Celular/metabolismo , Sistemas de Computación , Hibridomas/metabolismo , Animales , Anticuerpos/inmunología , Células CHO , Membrana Celular/inmunología , Cricetinae , Cricetulus , Células HEK293 , Humanos , Hibridomas/inmunología , Técnicas de Inmunoadsorción , Ratones , Receptores de IgG/inmunología
19.
Analyst ; 138(12): 3470-7, 2013 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-23653905

RESUMEN

The orientation of sensing molecules on solid phase biosensors has to be optimized to facilitate efficient binding of analytes. Since conventional observation methods (e.g., electron microscopy, atomic force microscopy, time-of-flight secondary ion mass spectrometry) require exaggerated machines and possess insufficient resolution for single molecule analyses, functional assays based on the reactivity to analytes have thus far been used for this optimization. However, it is not clear whether these assays can judge whether sensing molecules are fixed in an oriented-immobilization manner or not. Here, we describe that bio-nanocapsules of about 30 nm diameter, displaying approximately 120 molecules of a tandem form of the immunoglobulin (Ig) G Fc-binding Z domain (ZZ-BNCs), can discriminate between the Fc regions of IgGs fixed in an oriented-immobilization manner and those fixed randomly, thus facilitating the evaluation of the orientation of IgGs in immunosensors. Furthermore, in sandwich immunoassays, ZZ-BNCs can bind specifically to detection-IgGs fixed in an oriented-immobilization manner by antigen-capture IgG complexes, rather than to capture-IgGs fixed randomly onto a solid phase, allowing the simultaneous use of the same IgG as capture- and detection-IgGs. Thus, we demonstrate that ZZ-BNCs are a unique probe for evaluating the orientation of IgGs on a solid phase.


Asunto(s)
Anticuerpos Inmovilizados/química , Anticuerpos Inmovilizados/metabolismo , Técnicas Biosensibles/métodos , Sondas Moleculares/metabolismo , Nanocápsulas/química , Anticuerpos Inmovilizados/inmunología , Ensayo de Inmunoadsorción Enzimática , Humanos , Fragmentos Fc de Inmunoglobulinas/metabolismo , Inmunoglobulina G/metabolismo , Sondas Moleculares/química , Estructura Terciaria de Proteína , Proteína Estafilocócica A/química , Proteína Estafilocócica A/metabolismo , Propiedades de Superficie
20.
Microbiol Immunol ; 57(6): 470-7, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23773026

RESUMEN

An engineered bio-nanocapsule (BNC) comprising modified hepatitis B surface antigen L protein was used as a physical scaffold for envelope protein domain III (D3) of Japanese encephalitis virus (JEV). At the N terminus, the BNC contained a two-tandem repeat of the Z domain (ZZ) derived from Staphylococcus aureus protein A (ZZ-BNC). The Lys-rich ZZ moiety exposed on the surface of ZZ-BNC was used for chemical conjugation with the JEV D3 antigen, which had been expressed and purified from Escherichia coli. Immunization of mice with D3 loaded on the surface of ZZ-BNC (ZZ-BNC:D3) augmented serum IgG response against JEV and increased protection against lethal JEV infection. The present study suggests that innocuous recombinant antigens, when loaded on the surface of ZZ-BNC, can be transformed to immunogenic antigens.


Asunto(s)
Portadores de Fármacos/administración & dosificación , Virus de la Encefalitis Japonesa (Especie)/inmunología , Vacunas contra la Encefalitis Japonesa/inmunología , Nanocápsulas/administración & dosificación , Proteínas del Envoltorio Viral/inmunología , Animales , Anticuerpos Antivirales/sangre , Virus de la Encefalitis Japonesa (Especie)/genética , Escherichia coli/genética , Femenino , Inmunoglobulina G/sangre , Vacunas contra la Encefalitis Japonesa/administración & dosificación , Ratones , Ratones Endogámicos BALB C , Unión Proteica , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteína Estafilocócica A/genética , Proteína Estafilocócica A/metabolismo , Análisis de Supervivencia , Vacunación/métodos , Vacunas Sintéticas/administración & dosificación , Vacunas Sintéticas/inmunología , Proteínas del Envoltorio Viral/genética
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