RESUMEN
The metabolic fate of a compound can often determine the success of a new drug lead. Thus, significant effort is directed toward identifying the metabolites formed from a given molecule. Here, an automated and nontargeted procedure is introduced for detecting drug metabolites without authentic metabolite standards via the use of stable isotope labeling, liquid chromatography mass spectrometry (LC/MS), and high-performance computing. LC/MS of blood plasma extracts from rats that were administered a 1:1 mixture of acetaminophen (APAP) and (13)C6-APAP resulted in mass spectra that contained "twin" ions for drug metabolites that were not detected in control spectra (i.e., no APAP administered). Because of the development of a program (high-resolution twin-ion metabolite extraction; HiTIME) that can identify twin-ions in high-resolution mass spectra without centroiding (i.e., reduction of mass spectral peaks to single data points), 9 doublets corresponding to APAP metabolites were identified. This is nearly twice that obtained by use of existing programs that make use of centroiding to reduce computational cost under these conditions with a quadrupole time-of-flight mass spectrometer. By a manual search for all reported APAP metabolite ions, no additional twin-ion signals were assigned. These data indicate that all the major metabolites of APAP and multiple low-abundance metabolites (e.g., acetaminophen hydroxy- and methoxysulfate) that are rarely reported were detected. This methodology can be used to detect drug metabolites without prior knowledge of their identity. HiTIME is freely available from https://github.com/bjpop/HiTIME .
Asunto(s)
Acetaminofén/sangre , Automatización , Metodologías Computacionales , Acetaminofén/administración & dosificación , Acetaminofén/química , Acetaminofén/metabolismo , Animales , Cromatografía Liquida , Marcaje Isotópico , Masculino , Espectrometría de Masas , Ratas , Ratas Sprague-DawleyRESUMEN
The mechanistic links between climate and the environmental sensitivities of organisms occur through the microclimatic conditions that organisms experience. Here we present a dataset of gridded hourly estimates of typical microclimatic conditions (air temperature, wind speed, relative humidity, solar radiation, sky radiation and substrate temperatures from the surface to 1 m depth) at high resolution (~15 km) for the globe. The estimates are for the middle day of each month, based on long-term average macroclimates, and include six shade levels and three generic substrates (soil, rock and sand) per pixel. These data are suitable for deriving biophysical estimates of the heat, water and activity budgets of terrestrial organisms.