[A Case of Resection of T2N0 Colon Cancer with Synchronous Solitary Lung Metastasis].

A 73-year-old man was referred to our hospital for anemia. He underwent a colonoscopy; a 15-mm Ip polyp and a 30- mm type 1 lesion were found in the sigmoid colon. Pathological examination results indicated a well-differentiated adenocarcinoma. Thoracic computed tomography(CT)revealed a mass lesion 12 mm in diameter in the left lung lobe. The patient underwent a laparoscopic sigmoidectomy and D3 lymph node dissection and was discharged in a good condition. He then underwent a diagnostic-therapeutic segmental pulmonary resection for the pulmonary mass. Postoperative pathological findings indicated pT1b(SM), ly0, v0 and pT2(MP), ly1, v1, pN0 for the 2 lesions of the colon. The pulmonary mass was diagnosed as a metastatic adenocarcinoma based on immunostaining examination(CK7: negative, CK20: positive, TTF-1: negative, and CDX-2: positive). The patient is currently under follow-up as an outpatient without recurrence.

Deep sound-field denoiser: optically-measured sound-field denoising using deep neural network.

This paper proposes a deep sound-field denoiser, a deep neural network (DNN) based denoising of optically measured sound-field images. Sound-field imaging using optical methods has gained considerable attention due to its ability to achieve high-spatial-resolution imaging of acoustic phenomena that conventional acoustic sensors cannot accomplish. However, the optically measured sound-field images are often heavily contaminated by noise because of the low sensitivity of optical interferometric measurements to airborne sound. Here, we propose a DNN-based sound-field denoising method. Time-varying sound-field image sequences are decomposed into harmonic complex-amplitude images by using a time-directional Fourier transform. The complex images are converted into two-channel images consisting of real and imaginary parts and denoised by a nonlinear-activation-free network. The network is trained on a sound-field dataset obtained from numerical acoustic simulations with randomized parameters. We compared the method with conventional ones, such as image filters, a spatiotemporal filter, and other DNN architectures, on numerical and experimental data. The experimental data were measured by parallel phase-shifting interferometry and holographic speckle interferometry. The proposed deep sound-field denoiser significantly outperformed the conventional methods on both the numerical and experimental data. Code is available on GitHub (https://github.com/nttcslab/deep-sound-field-denoiser).

Determination of microphone acoustic center from sound field projection measured by optical interferometry.

This article presents a method for determining the acoustic center of a microphone from a sound field measured by optical interferometry. The acoustic center defines the equivalent point source position of a microphone serving as a sound source where the spherical waveform starts to diverge. The value is used to determine the effective distance between microphones for free-field reciprocity calibration. Conventionally, it is determined from the inverse distance law properties of a point source using the transfer function method. In this study, the acoustic center was determined from the projection of the sound field of the microphone. Parallel phase-shifting interferometry was used to measure the line integration of the sound pressure from a microphone. The acoustic center is determined as the position where the squared error between the measured data and the projection model of a point source is minimized. Experiments with the B&K 4180 (Brüel & Kjær, Nærum, Denmark) microphone were performed for frequencies from 10 to 50 kHz. The best acoustic center estimation was obtained at a microphone distance of 0 mm, with a difference of 0.17 mm to the IEC 61094-3 value and 0.36 mm to the Barrera-Figueroa et al. [J. Acoust. Soc. Am. 120(5), 2668-2675 (2006)] result at a measurement frequency of 20 kHz.

Speckle holographic imaging of a sound field using Fresnel lenses.

In this Letter, we propose to use Fresnel lenses for holographic sound-field imaging. Although a Fresnel lens has never been used for sound-field imaging mainly due to its low imaging quality, it has several desired properties, including thinness, lightweight, low cost, and ease of making a large aperture. We constructed an optical holographic imaging system composed of two Fresnel lenses used for magnification and demagnification of the illuminating beam. A proof-of-concept experiment verified that the sound-field imaging with Fresnel lenses is possible by using the spatiotemporally harmonic nature of sound.

Study of optical emission spectroscopy using modified Boltzmann plot in dual-frequency synchronized pulsed capacitively coupled discharges with DC bias at low-pressure in Ar/O2/C4F8 plasma etching process.

We consider the corona model and local thermal equilibrium approximations of a real plasma to measure the electron temperature (Te) and density (ne), respectively, using the optical emission spectroscopy (OES) method in dual-frequency pulsed capacitively coupled plasmas (CCPs) in a reactive mixture of Ar/O2/C4F8 at a low operating pressure. The operation conditions such as DC continuous and synchronized were used for the study and plasma characterization for the intended plasma application such as high aspect ratio etching (HARE). We show that the present plasma conditions are dominated by a corona balance rather than the supremacy of multi-step excitation. This fact has enabled us to utilize the modified Boltzmann plot technique to evaluate the Te values. In the second method, we simultaneously used the Boltzmann and Saha equations to determine the ne value using the line intensity ratio and the value of Te. Time-resolved measurements of Te and ne were performed for completeness, and the insight of the pulsed discharge was investigated. Time evolution of ne and Te using the OES method revealed a similar trend in the change of plasma parameters, indicating electron impact ionization during the pulse on phase. It was seen that ne in the afterglow speedily decreased within a short time of ∼5 µs. Analysis suggests the formation of afterglow plasmas, which are composed of positive and negative ions with very low electron density. The results revealed that the DC-synchronized operation could be useful for plasma application such as HARE due to different plasma characteristics. It also suggests the production of ion-ion plasmas by the effective utilization of negative ions in the afterglow phase. The corona balance condition was validated in our experiments, and the results were compared with the existing literature.

Low-noise optical measurement of sound using midfringe locked interferometer with differential detection.

A midfringe locked interferometer with differential detection is proposed for non-contact optical sound measurement, and the equivalent noise level of approximately 0 dB SPL/Hz is achieved. The noise level of the proposed method is 30 dB lower than that of a very recent laser Doppler vibrometer and close to that of a quarter-inch measurement microphone. The midfringe locking stabilizes the optical interferometer against slow environmental fluctuations and enables detection of the acoustic signal directly from optical intensity. The differential detection method eliminates laser intensity noise, which is a dominant noise source in optical interferometers. The noise level of the constructed system was approximately 10 dB above the optical shot-noise (the classical detection limit attributed to the quantum nature of light) at frequencies higher than 2 kHz. Further noise reduction by several available methods could lead to optical measurements that are more sensitive than measurements by microphones. In addition, the constructed interferometer is used to reconstruct sound fields generated by a half-inch laboratory standard microphone used as a transmitter. The proposed method will be a powerful tool for measuring small-amplitude sound fields where it has been challenging to use existing methods.

Non-thermal plasma-activated lactate solution kills U251SP glioblastoma cells in an innate reductive manner with altered metabolism.

Ringer's lactate solution irradiated by non-thermal plasma, comprised of radicals, electrons, and ions, is defined as plasma-activated lactate (PAL). PAL exhibited antitumor effects in glioblastoma U251SP cells, which we termed PAL-specific regulated cell death. In contrast to the oxidative stress condition typical of cells incubated in plasma-activated medium (PAM), U251SP cells treated with Ringer's lactate solution or PAL exhibited changes in intracellular metabolites that were reductive in the redox state, as measured by the ratio of oxidative/reductive glutathione concentrations. In the metabolomic profiles of PAL-treated cells, the generation of acetyl-CoA increased for lipid metabolism from alanine and asparagine. PAL thus induces regulated death of U251SP glioblastoma cells in more innate microenvironments than PAM.

Seeing the sound of castanets: Acoustic resonances between shells captured by high-speed optical visualization with 1-mm resolution.

Sound fields radiated from the castanet, a Spanish percussive instrument comprising two shells, were optically visualized. A measurement system, which used parallel phase-shifting interferometry and a high-speed polarization camera, enabled the capture of instantaneous sound fields around the castanets, while the castanets were played, with the spatial resolution of 1.1 mm and frame rate of 100 000 fps. By carefully aligning the tilt of the castanets, the sound fields within the 1-mm gaps between both the shells were captured. From the visualization results, two acoustic resonances between the shells were identified. The first mode appeared between 1000 and 2000 Hz and exhibited a frequency chirp of several hundred hertz for several milliseconds after the impact. This can be explained by the Helmholtz resonance with a time-varying resonator shape, which is caused by the movement of the shells after impact. The second mode showed a resonance pattern with a single nodal diameter at the center of the shells, i.e., the standing wave mode caused by the interior volume. These physical phenomena involved in the sound radiation were identified owing to the unique features of the optical imaging method, such as contactless nature and millimeter-resolution imaging of instantaneous pressure fields.

Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma.

Non-equilibrium atmospheric pressure plasma (NEAPP) is a mixture of radicals, electrons, anions, cations and light at near body temperature. Plasma-activated medium (PAM) is realized using NEAPP provided by engineered devices and irradiated to a cell culture medium for a period of 600 s. Glioblastoma cells U251SP cultivated in PAM previously indicated that antitumor effects induced PAM-specific apoptotic cell-death. Metabolomic profiles of a hundred intracellular metabolites were analyzed using capillary electrophoresis mass spectrometry. The metabolomic profiles of the PAM-treated U251SP cells were changed significantly with inhibition of the glycolysis pathway and with enhancement of the pentose phosphate pathway.

Molecular mechanisms of non-thermal plasma-induced effects in cancer cells.

Plasma is the fourth state of matter with higher energy than gas; non-thermal plasma (NTP) is currently available. As NTP is useful in sterilization, promoting wound healing and cancer treatments, the molecular mechanisms of plasma-induced effects in living cells and microorganisms are of significant interest in plasma medicine with medical-engineering collaboration. Molecular mechanisms of plasma-induced effects in cancer cells will be described in this minireview. Both direct and indirect methods to treat cancer cells with NTP have been developed. NTP interacts directly with not only cancer cells but also the liquids surrounding cancer cells and the immune cells that target them. Reactive oxygen and nitrogen species play key roles in NTP-induced effects; however, other mechanisms have been suggested. The complex interactions between NTP, cells and liquids have been extensively studied. In the future, details regarding NTP-induced effects on gene regulatory networks, signaling networks, and metabolic networks will be elucidated.

Time-directional filtering of wrapped phase for observing transient phenomena with parallel phase-shifting interferometry.

Recent development of parallel phase-shifting interferometry (PPSI) enables accurate measurement of time-varying phase maps. By combining a high-speed camera with PPSI, it became possible to observe not only time-varying but also fast phenomena including fluid flow and sound in air. In such observation, one has to remove static phase (time-invariant or slowly-varying phase unrelated to the phenomena of interest) from the observed phase maps. Ordinarily, a signal processing method for eliminating the static phase is utilized after phase unwrapping to avoid the 2π discontinuity which can be a source of error. In this paper, it is shown that such phase unwrapping is not necessary for the high-speed observation, and a time-directional filtering method is proposed for removing the static phase directly from the wrapped phase without performing phase unwrapping. In addition, experimental results of simultaneously visualizing flow and sound with 42 000 fps are shown to illustrate how the time-directional filtering changes the appearance. A MATLAB code is included within the paper (also in https://goo.gl/N4wzdp) for aiding the understanding of the proposed method.

Optical visualization of a fluid flow via the temperature controlling method.

In this Letter, a visualization method of a fluid flow through temperature control is proposed. The proposed method enables us to visualize an invisible fluid flow by controlling the temperature so that its visibility can be easily adjusted. Such ability of adjusting appearance is effective for visualizing the phenomena consisting of multiple physical processes. In order to verify the validity of the proposed method, the measurement experiment of visualization of both flow and sound in air using parallel phase-shifting interferometry, which is a similar condition to the previous research [Opt. Lett.43, 991 (2018)OPLEDP0146-959210.1364/OL.43.000991], was conducted.

Simultaneous imaging of flow and sound using high-speed parallel phase-shifting interferometry.

In this Letter, simultaneous imaging of flow and sound by using parallel phase-shifting interferometry and a high-speed polarization camera is proposed. The proposed method enables the visualization of flow and sound simultaneously by using the following two factors: (i) injection of the gas, whose density is different from the surrounding air, makes the flow visible to interferometry, and (ii) time-directional processing is applied for extracting the small-amplitude sound wave from the high-speed flow video. An experiment with a frame rate of 42,000 frames per second for visualizing the flow and sound emitted from a whistle was conducted. By applying time-directional processing to the obtained video, both flow emitted from the slit of the whistle and a spherical sound wave of 8.7 kHz were successively captured.

Plasma-activated medium (PAM) kills human cancer-initiating cells.

Medical non-thermal plasma (NTP) treatments for various types of cancers have been reported. Cells with tumorigenic potential (cancer-initiating cells; CICs) are few in number in many types of tumors. CICs efficiently eliminate anti-cancer chemicals and exhibit high-level aldehyde dehydrogenase (ALDH) activity. We previously examined the effects of direct irradiation via NTP on cancer cells; even though we targeted CICs expressing high levels of ALDH, such treatment affected both non-CICs and CICs. Recent studies have shown that plasma-activated medium (PAM) (culture medium irradiated by NTP) selectively induces apoptotic death of cancer but not normal cells. Therefore, we explored the anti-cancer effects of PAM on CICs among endometrioid carcinoma and gastric cancer cells. PAM reduced the viability of cells expressing both low and high levels of ALDH. Combined PAM/cisplatin appeared to kill cancer cells more efficiently than did PAM or cisplatin alone. In a mouse tumor xenograft model, PAM exerted an anti-cancer effect on CICs. Thus, our results suggest that PAM effectively kills both non-CICs and CICs, as does NTP. Therefore, PAM may be a useful new anti-cancer therapy, targeting various cancer cells including CICs.

Intracellular-molecular changes in plasma-irradiated budding yeast cells studied using multiplex coherent anti-Stokes Raman scattering microscopy.

Interactions between non-equilibrium atmospheric-pressure plasma (NEAPP) and living cells were examined using multiplex coherent anti-Stokes Raman scattering (CARS) microscopy. Our multiplex CARS analyses revealed that NEAPP irradiation generates short-lived radicals that induce a decrease in the mitochondrial activity of budding yeast cells.

Simple, flexible, and accurate phase retrieval method for generalized phase-shifting interferometry.

This paper presents a non-iterative phase retrieval method from randomly phase-shifted fringe images. By combining the hyperaccurate least squares ellipse fitting method with the subspace method (usually called the principal component analysis), a fast and accurate phase retrieval algorithm is realized. The proposed method is simple, flexible, and accurate. It can be easily coded without iteration, initial guess, or tuning parameter. Its flexibility comes from the fact that totally random phase-shifting steps and any number of fringe images greater than two are acceptable without any specific treatment. Finally, it is accurate because the hyperaccurate least squares method and the modified subspace method enable phase retrieval with a small error as shown by the simulations. A MATLAB code, which is used in the experimental section, is provided within the paper to demonstrate its simplicity and easiness.

Helium-based cold atmospheric plasma-induced reactive oxygen species-mediated apoptotic pathway attenuated by platinum nanoparticles.

Plasma is generated by ionizing gas molecules. Helium (He)-based cold atmospheric plasma (CAP) was generated using a high-voltage power supply with low-frequency excitation (60 Hz at 7 kV) and He flow at 2 l/min. Platinum nanoparticles (Pt-NPs) are potent antioxidants due to their unique ability to scavenge superoxides and peroxides. These features make them useful for the protection against oxidative stress-associated pathologies. Here, the effects of Pt-NPs on He-CAP-induced apoptosis and the underlying mechanism were examined in human lymphoma U937 cells. Apoptosis was measured after cells were exposed to He-CAP in the presence or absence of Pt-NPs. The effects of combined treatment were determined by observing the changes in intracellular reactive oxygen species (ROS) and both mitochondrial and Fas dependent pathway. The results indicate that Pt-NPs substantially scavenge He-CAP-induced superoxides and peroxides and inhibit all the pathways involved in apoptosis execution. This might be because of the SOD/catalase mimetic effects of Pt-NPs. These results showed that the Pt-NPs can induce He-CAP desensitization in human lymphoma U937 cells.

Improving principal component analysis based phase extraction method for phase-shifting interferometry by integrating spatial information.

Phase extraction methods based on the principal component analysis (PCA) can extract objective phase from phase-shifted fringes without any prior knowledge about their shift steps. Although it is fast and easy to implement, many fringe images are needed for extracting the phase accurately from noisy fringes. In this paper, a simple extension of the PCA method for reducing extraction error is proposed. It can effectively reduce influence from random noise, while most of the advantages of the PCA method is inherited because it only modifies the construction process of the data matrix from fringes. Although it takes more time because size of the data matrix to be decomposed is larger, computational time of the proposed method is shown to be reasonably fast by using the iterative singular value decomposition algorithm. Numerical experiments confirmed that the proposed method can reduce extraction error even when the number of interferograms is small.

High-speed imaging of sound using parallel phase-shifting interferometry.

Sound-field imaging, the visualization of spatial and temporal distribution of acoustical properties such as sound pressure, is useful for understanding acoustical phenomena. This study investigated the use of parallel phase-shifting interferometry (PPSI) with a high-speed polarization camera for imaging a sound field, particularly high-speed imaging of propagating sound waves. The experimental results showed that the instantaneous sound field, which was generated by ultrasonic transducers driven by a pure tone of 40 kHz, was quantitatively imaged. Hence, PPSI can be used in acoustical applications requiring spatial information of sound pressure.

Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium.

Non-equilibrium atmospheric pressure plasmas generate a high electron density (on the order of 10(16) electrons per cm(-3)) using Ar gas. Culture medium in air at room temperature was plasma-irradiated for several hundred seconds. Tens of micromolar hydrogen peroxide (H2O2) and millimolar levels of nitrous ion (NO2(-)) were detected in the plasma-irradiated culture medium (plasma activated medium; PAM) and selectively induced the apoptotic death of glioblastoma tumor cells, but did not kill normal mammary epithelial cells. A similar antitumor effect was induced by spiking the medium with comparable concentrations of H2O2 and NO2(-). The PAM remained still a somewhat difference that it should also be assessed for understanding other latent mechanisms.