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1.
Int J Mol Sci ; 25(5)2024 Feb 27.
Artículo en Inglés | MEDLINE | ID: mdl-38474002

RESUMEN

Psoriasis is a chronic inflammatory skin disease characterized by the rapid abnormal growth of skin cells in the epidermis, driven by an overactive immune system. Consequently, a complex interplay among epidermal cells, immune cells, and sensory neurons contributes to the development and progression of psoriasis. In these cellular contexts, various ion channels, such as acetylcholine receptors, TRP channels, Ca2+ release-activated channels, chloride channels, and potassium channels, each serve specific functions to maintain the homeostasis of the skin. The dysregulation of ion channels plays a major role in the pathophysiology of psoriasis, affecting various aspects of epidermal cells, immune responses, and sensory neuron signaling. Impaired function of ion channels can lead to altered calcium signaling, inflammation, proliferation, and sensory signaling, all of which are central features of psoriasis. This overview summarizes the pathophysiological roles of ion channels in epidermal cells, immune cells, and sensory neurons during early and late psoriatic processes, thereby contributing to a deeper understanding of ion channel involvement in the interplay of psoriasis and making a crucial advance toward more precise and personalized approaches for psoriasis treatment.


Asunto(s)
Queratinocitos , Psoriasis , Humanos , Queratinocitos/fisiología , Epidermis , Células Epidérmicas , Células Receptoras Sensoriales , Canales Iónicos
2.
Nat Chem Biol ; 16(8): 876-886, 2020 08.
Artículo en Inglés | MEDLINE | ID: mdl-32451509

RESUMEN

The orphan nuclear receptor Nurr1 is critical for the development, maintenance and protection of midbrain dopaminergic (mDA) neurons. Here we show that prostaglandin E1 (PGE1) and its dehydrated metabolite, PGA1, directly interact with the ligand-binding domain (LBD) of Nurr1 and stimulate its transcriptional function. We also report the crystallographic structure of Nurr1-LBD bound to PGA1 at 2.05 Å resolution. PGA1 couples covalently to Nurr1-LBD by forming a Michael adduct with Cys566, and induces notable conformational changes, including a 21° shift of the activation function-2 helix (H12) away from the protein core. Furthermore, PGE1/PGA1 exhibit neuroprotective effects in a Nurr1-dependent manner, prominently enhance expression of Nurr1 target genes in mDA neurons and improve motor deficits in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned mouse models of Parkinson's disease. Based on these results, we propose that PGE1/PGA1 represent native ligands of Nurr1 and can exert neuroprotective effects on mDA neurons, via activation of Nurr1's transcriptional function.


Asunto(s)
Alprostadil/metabolismo , Miembro 2 del Grupo A de la Subfamilia 4 de Receptores Nucleares/metabolismo , Prostaglandinas A/metabolismo , Animales , Línea Celular Tumoral , Cristalografía por Rayos X , Dopamina/metabolismo , Humanos , Ligandos , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Neuronas/metabolismo , Fármacos Neuroprotectores/farmacología , Miembro 2 del Grupo A de la Subfamilia 4 de Receptores Nucleares/química , Miembro 2 del Grupo A de la Subfamilia 4 de Receptores Nucleares/genética , Unión Proteica , Ratas , Transducción de Señal , Transcripción Genética
3.
Proc Natl Acad Sci U S A ; 116(25): 12494-12499, 2019 06 18.
Artículo en Inglés | MEDLINE | ID: mdl-31147466

RESUMEN

Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated chloride channel, mediates the Ca2+-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, Ano1-deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain.


Asunto(s)
Anoctamina-1/fisiología , Encéfalo/citología , Encéfalo/embriología , Neuroglía/citología , Animales , Anoctamina-1/genética , Encéfalo/metabolismo , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Cloruros/metabolismo , Regulación hacia Abajo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Neuroglía/metabolismo , Regulación hacia Arriba
4.
Int J Mol Sci ; 24(1)2022 Dec 30.
Artículo en Inglés | MEDLINE | ID: mdl-36614120

RESUMEN

Electroceuticals refer to various forms of electronic neurostimulators used for therapy. Interdisciplinary advances in medical engineering and science have led to the development of the electroceutical approach, which involves therapeutic agents that specifically target neural circuits, to realize precision therapy for Alzheimer's disease (AD). To date, extensive studies have attempted to elucidate the disease-modifying effects of electroceuticals on areas in the brain of a patient with AD by the use of various physical stimuli, including electric, magnetic, and electromagnetic waves as well as ultrasound. Herein, we review non-invasive stimulatory systems and their effects on ß-amyloid plaques and tau tangles, which are pathological molecular markers of AD. Therefore, this review will aid in better understanding the recent technological developments, applicable methods, and therapeutic effects of electronic stimulatory systems, including transcranial direct current stimulation, 40-Hz gamma oscillations, transcranial magnetic stimulation, electromagnetic field stimulation, infrared light stimulation and ionizing radiation therapy, and focused ultrasound for AD.


Asunto(s)
Enfermedad de Alzheimer , Estimulación Transcraneal de Corriente Directa , Humanos , Enfermedad de Alzheimer/terapia , Enfermedad de Alzheimer/patología , Péptidos beta-Amiloides/farmacología , Proteínas tau , Placa Amiloide/patología , Ovillos Neurofibrilares/patología
5.
Angew Chem Int Ed Engl ; 60(19): 10563-10567, 2021 05 03.
Artículo en Inglés | MEDLINE | ID: mdl-33565220

RESUMEN

The carbon nanotube (CNT) yarn supercapacitor has high potential for in vivo energy storage because it can be used in aqueous environments and stitched to inner parts of the body, such as blood vessels. The biocompatibility issue for frequently used pseudocapacitive materials, such as metal oxides, is controversial in the human body. Here, we report an implantable CNT yarn supercapacitor inspired by the cellular redox system. In all living cells, nicotinamide adenine dinucleotide (NAD) is a key redox biomolecule responsible for cellular energy transduction to produce adenosine triphosphate (ATP). Based on this redox system, CNT yarn electrodes were fabricated by inserting a twist in CNT sheets with electrochemically deposited NAD and benzoquinone for redox shuttling. Consequently, the NAD/BQ/CNT yarn electrodes exhibited the maximum area capacitance (55.73 mF cm-2 ) under physiological conditions, such as phosphate-buffered saline and serum. In addition, the yarn electrodes showed a negligible loss of capacitance after 10 000 repeated charge/discharge cycles and deformation tests (bending/knotting). More importantly, NAD/BQ/CNT yarn electrodes implanted into the abdominal cavity of a rat's skin exhibited the stable in vivo electrical performance of a supercapacitor. Therefore, these findings demonstrate a redox biomolecule-applied platform for implantable energy storage devices.


Asunto(s)
Materiales Biocompatibles/metabolismo , Nanotubos de Carbono/química , Piel/metabolismo , Animales , Materiales Biocompatibles/química , Oxidación-Reducción , Ratas , Piel/citología
6.
J Neuroinflammation ; 17(1): 30, 2020 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-31969159

RESUMEN

Arachidonic acid-derived prostaglandins not only contribute to the development of inflammation as intercellular pro-inflammatory mediators, but also promote the excitability of the peripheral somatosensory system, contributing to pain exacerbation. Peripheral tissues undergo many forms of diseases that are frequently accompanied by inflammation. The somatosensory nerves innervating the inflamed areas experience heightened excitability and generate and transmit pain signals. Extensive studies have been carried out to elucidate how prostaglandins play their roles for such signaling at the cellular and molecular levels. Here, we briefly summarize the roles of arachidonic acid-derived prostaglandins, focusing on four prostaglandins and one thromboxane, particularly in terms of their actions on afferent nociceptors. We discuss the biosynthesis of the prostaglandins, their specific action sites, the pathological alteration of the expression levels of related proteins, the neuronal outcomes of receptor stimulation, their correlation with behavioral nociception, and the pharmacological efficacy of their regulators. This overview will help to a better understanding of the pathological roles that prostaglandins play in the somatosensory system and to a finding of critical molecular contributors to normalizing pain.


Asunto(s)
Ácido Araquidónico/metabolismo , Nocicepción/fisiología , Dolor/metabolismo , Prostaglandinas/metabolismo , Animales , Humanos
7.
Biochem Biophys Res Commun ; 495(1): 1208-1213, 2018 01 01.
Artículo en Inglés | MEDLINE | ID: mdl-29183725

RESUMEN

Harmful maternal behaviors, such as drinking and smoking, negatively affect embryonic development. In contrast, regular maternal exercise is believed to be beneficial to the fetus. Although it is not surprising that voluntary physical activities are advantageous to fetal development, it remains unclear whether involuntary maternal exercise has similar effects. The constituents of the amniotic fluid (AF) inevitably reflect the maternal plasma. Therefore, it is speculated that exercise-induced changes in maternal plasma can influence fetal development through changes in AF composition. Therefore, we investigated the effect of AF on fetal neurodevelopment and changes in AF composition after involuntary swimming exercise (SE) in an animal model. We found that there was a severe reduction in the number of embryos implanted in the uterus of SE rats. Surprisingly, cortisol level (an inducible stress hormone) was significantly increased in AF from SE rats. In contrast, the growth factors NGF and VEGF were reduced in the AF from SE rats. In the cultured embryonic cortical neurons, the treatment of control (CTL) rat-derived AF significantly increased the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2)-mediated signaling that is essential for fetal neurodevelopment. However, the AF extracted from SE rats reversely suppressed the phosphorylation of ERK1/2-mediated signaling in cortical neurons compared to that in CTL rats. Indeed, the co-treatment with control AF and dexamethasone, a synthetic glucocorticoid, inhibited the phosphorylation of ERK1/2 in a dose-dependent manner. This finding suggests that the inhibition of ERK1/2 can be attributed to increased cortisol level in AF resulting from involuntary exercise. Therefore, involuntary maternal swimming increases cortisol level in AF, which ultimately hinders the ERK1/2 signaling pathway in embryonic neurons. These findings also suggest that involuntary maternal exercise can have undesirable effects on fetal neurodevelopment, which is potentially mediated by elevated AF cortisol level.


Asunto(s)
Líquido Amniótico/metabolismo , Desarrollo Embrionario/fisiología , Hidrocortisona/metabolismo , Sistema de Señalización de MAP Quinasas/fisiología , Neuronas/metabolismo , Preñez/metabolismo , Estrés Fisiológico/fisiología , Animales , Femenino , Regulación del Desarrollo de la Expresión Génica , Condicionamiento Físico Animal/métodos , Embarazo , Ratas , Ratas Sprague-Dawley , Natación , Volición/fisiología
8.
Proc Natl Acad Sci U S A ; 112(31): 9722-7, 2015 Aug 04.
Artículo en Inglés | MEDLINE | ID: mdl-26153424

RESUMEN

Benign prostatic hyperplasia (BPH) is characterized by an enlargement of the prostate, causing lower urinary tract symptoms in elderly men worldwide. However, the molecular mechanism underlying the pathogenesis of BPH is unclear. Anoctamin1 (ANO1) encodes a Ca(2+)-activated chloride channel (CaCC) that mediates various physiological functions. Here, we demonstrate that it is essential for testosterone-induced BPH. ANO1 was highly amplified in dihydrotestosterone (DHT)-treated prostate epithelial cells, whereas the selective knockdown of ANO1 inhibited DHT-induced cell proliferation. Three androgen-response elements were found in the ANO1 promoter region, which is relevant for the DHT-dependent induction of ANO1. Administration of the ANO1 blocker or Ano1 small interfering RNA, inhibited prostate enlargement and reduced histological abnormalities in vivo. We therefore concluded that ANO1 is essential for the development of prostate hyperplasia and is a potential target for the treatment of BPH.


Asunto(s)
Canales de Cloruro/metabolismo , Proteínas de Neoplasias/metabolismo , Próstata/metabolismo , Próstata/patología , Testosterona/farmacología , Animales , Anoctamina-1 , Calcio/farmacología , Canales de Calcio/metabolismo , Proliferación Celular/efectos de los fármacos , Inmunoprecipitación de Cromatina , Dihidrotestosterona/farmacología , Modelos Animales de Enfermedad , Células Epiteliales/efectos de los fármacos , Células Epiteliales/metabolismo , Técnicas de Silenciamiento del Gen , Genes Reporteros , Humanos , Hiperplasia , Inyecciones , Activación del Canal Iónico/efectos de los fármacos , Luciferasas/metabolismo , Masculino , Regiones Promotoras Genéticas/genética , Próstata/efectos de los fármacos , Hiperplasia Prostática/metabolismo , Hiperplasia Prostática/patología , ARN Interferente Pequeño/metabolismo , Ratas Wistar , Elementos de Respuesta/genética , Taninos/farmacología , Regulación hacia Arriba/efectos de los fármacos
9.
Phytother Res ; 32(4): 616-624, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29250842

RESUMEN

Poncirus trifoliata (L.) Raf. belongs to the family Rutaceae in the genus Poncirus. Its fruits are widely used to alleviate symptoms of various disorders. The mature fruit (MF) possesses anticancer and antiinflammatory activities. Extracts of the dried, immature fruit, Poncirus fructus (PF) are widely used as a traditional medicine for ameliorating symptoms of digestive dysfunction in East Asia. Molecular and cellular mechanisms underlying the effects of MF and PF extracts on cancer, inflammation, and gastrointestinal disorders have been extensively studied in the past decade. This review summarizes recent findings on the anticancer and antiinflammatory effects of MF and the prokinetic effects of PF. Although the therapeutic effects of MF and PF have been clearly elucidated, in-depth further clinical studies are still required to completely verify the clinical efficacy and safety of the fruits of P. trifoliata (L.) Raf.


Asunto(s)
Anomalías del Sistema Digestivo/tratamiento farmacológico , Frutas/química , Inflamación/tratamiento farmacológico , Neoplasias/tratamiento farmacológico , Extractos Vegetales/química , Poncirus/química , Humanos
10.
J Neurosci ; 35(34): 11811-23, 2015 Aug 26.
Artículo en Inglés | MEDLINE | ID: mdl-26311765

RESUMEN

Bipolar disorder (BD) is a psychiatric disease that causes mood swings between manic and depressed states. Although genetic linkage studies have shown an association between BD and TRPM2, a Ca(2+)-permeable cation channel, the nature of this association is unknown. Here, we show that D543E, a mutation of Trpm2 that is frequently found in BD patients, induces loss of function. Trpm2-deficient mice exhibited BD-related behavior such as increased anxiety and decreased social responses, along with disrupted EEG functional connectivity. Moreover, the administration of amphetamine in wild-type mice evoked a notable increase in open-field activity that was reversed by the administration of lithium. However, the anti-manic action of lithium was not observed in the Trpm2(-/-) mice. The brains of Trpm2(-/-) mice showed a marked increase in phosphorylated glycogen synthase kinase-3 (GSK-3), a key element in BD-like behavior and a target of lithium. In contrast, activation of TRPM2 induced the dephosphorylation of GSK-3 via calcineurin, a Ca(2+)-dependent phosphatase. Importantly, the overexpression of the D543E mutant failed to induce the dephosphorylation of GSK-3. Therefore, we conclude that the genetic dysfunction of Trpm2 causes uncontrolled phosphorylation of GSK-3, which may lead to the pathology of BD. Our findings explain the long-sought etiologic mechanism underlying the genetic link between Trpm2 mutation and BD. SIGNIFICANCE STATEMENT: Bipolar disorder (BD) is a mental disorder that causes changes in mood and the etiology is still unknown. TRPM2 is highly associated with BD; however, its involvement in the etiology of BD is still unknown. We show here that TRPM2 plays a central role in causing the pathology of BD. We found that D543E, a mutation of Trpm2 frequently found in BD patients, induces the loss of function. Trpm2-deficient mice exhibited mood disturbances and impairments in social cognition. TRPM2 actively regulates the phosphorylation of GSK-3, which is a main target of lithium, a primary medicine for treating BD. Therefore, abnormal regulation of GSK-3 by hypoactive TRPM2 mutants accounts for the pathology of BD, providing the possible link between BD and TRPM2.


Asunto(s)
Trastorno Bipolar/metabolismo , Encéfalo/metabolismo , Predisposición Genética a la Enfermedad , Glucógeno Sintasa Quinasa 3/metabolismo , Canales Catiónicos TRPM/fisiología , Animales , Trastorno Bipolar/genética , Línea Celular Tumoral , Activación Enzimática/fisiología , Predisposición Genética a la Enfermedad/genética , Glucógeno Sintasa Quinasa 3/genética , Células HEK293 , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Canales Catiónicos TRPM/genética
11.
Pflugers Arch ; 467(8): 1677-87, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-25231974

RESUMEN

Anoctamin 1 (ANO1)/TMEM16A is a Cl(-) channel activated by intracellular Ca(2+) mediating numerous physiological functions. However, little is known of the ANO1 activation mechanism by Ca(2+). Here, we demonstrate that two helices, "reference" and "Ca(2+) sensor" helices in the third intracellular loop face each other with opposite charges. The two helices interact directly in a Ca(2+)-dependent manner. Positively and negatively charged residues in the two helices are essential for Ca(2+)-dependent activation because neutralization of these charges change the Ca(2+) sensitivity. We now predict that the Ca(2+) sensor helix attaches to the reference helix in the resting state, and as intracellular Ca(2+) rises, Ca(2+) acts on the sensor helix, which repels it from the reference helix. This Ca(2+)-dependent push-pull conformational change would be a key electromechanical movement for gating the ANO1 channel. Because chemical activation of ANO1 is viewed as an alternative means of rescuing cystic fibrosis, understanding its gating mechanism would be useful in developing novel treatments for cystic fibrosis.


Asunto(s)
Calcio/metabolismo , Canales de Cloruro/metabolismo , Activación del Canal Iónico , Anoctamina-1 , Sitios de Unión , Canales de Cloruro/química , Canales de Cloruro/genética , Células HEK293 , Humanos , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Mutación , Unión Proteica , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Relación Estructura-Actividad , Resonancia por Plasmón de Superficie , Propiedades de Superficie , Transfección
12.
Biol Res ; 48: 44, 2015 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-26243199

RESUMEN

BACKGROUND: The fetus is surrounded by the amniotic fluid (AF) contained by the amniotic sac of the pregnant female. The AF is directly conveyed to the fetus during pregnancy. Although AF has recently been reported as an untapped resource containing various substances, it remains unclear whether the AF could influence fetal neurodevelopment. RESULTS: We used AF that was extracted from embryos at 16 days in pregnant SD rat and exposed the AF to the neural cells derived from the embryos of same rat. We found that the treatment of AF to cortical neurons increased the phosphorylation in ERK1/2 that is necessary for fetal neurodevelopment, which was inhibited by the treatment of MEK inhibitors. Moreover, we found the subsequent inhibition of glycogen synthase kinase-3 (GSK-3), which is an important determinant of cell fate in neural cells. Indeed, AF increased the neural clustering of cortical neurons, which revealed that the clustered cells were proliferating neural progenitor cells. Accordingly, we confirmed the ability of AF to increase the neural progenitor cells through neurosphere formation. Furthermore, we showed that the ERK/GSK-3 pathway was involved in AF-mediated neurosphere enlargement. CONCLUSIONS: Although the placenta mainly supplies oxygenated blood, nutrient substances for fetal development, these findings further suggest that circulating-AF into the fetus could affect fetal neurodevelopment via MAP kinases-derived GSK-3 pathway during pregnancy. Moreover, we suggest that AF could be utilized as a valuable resource in the field of regenerative medicine.


Asunto(s)
Líquido Amniótico/fisiología , Glucógeno Sintasa Quinasa 3/metabolismo , Sistema de Señalización de MAP Quinasas/fisiología , Células-Madre Neurales/fisiología , Animales , Diferenciación Celular , Femenino , Glucógeno Sintasa Quinasa 3/antagonistas & inhibidores , Células-Madre Neurales/citología , Fosforilación/efectos de los fármacos , Embarazo , Ratas , Ratas Sprague-Dawley , Transducción de Señal/fisiología
13.
Pflugers Arch ; 466(10): 1987-98, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24413888

RESUMEN

Intracellular Ca(2+) signal is a key regulator of axonal growth during brain development. As transient receptor potential (TRP) channels are permeable to Ca(2+) and mediate numerous brain functions, it is conceivable that many TRP channels would regulate neuronal differentiation. We therefore screened TRP channels that are involved in the regulation of neurite growth. Among the TRP channels, the Trpm2 level was inversely associated with neurite growth. TRPM2 was highly expressed in embryonic brain. Pharmacological perturbation or knockdown of TRPM2 markedly increased the axonal growth, whereas its overexpression inhibited the axonal growth. Addition of ADP ribose, an endogenous activator of TRPM2, to PC12 cells significantly repressed the axonal growth. TRPM2 was actively involved in the neuronal retraction induced by cerebrospinal fluid-rich lysophosphatidic acid (LPA). More importantly, neurons isolated from the brain of Trpm2-deficient mice have significantly longer neurites with a greater number of spines than those obtained from the brain of wild-type mice. Therefore, we conclude that TRPM2 mediates the LPA-induced suppression of axonal growth, which provides a long-sought mechanism underlying the effect of LPA on neuronal development.


Asunto(s)
Encéfalo/metabolismo , Neuritas/metabolismo , Neurogénesis , Canales Catiónicos TRPM/metabolismo , Adenosina Difosfato Ribosa/farmacología , Animales , Encéfalo/citología , Encéfalo/embriología , Células Cultivadas , Células HEK293 , Humanos , Lisofosfolípidos/farmacología , Ratones , Neuritas/efectos de los fármacos , Células PC12 , Ratas , Canales Catiónicos TRPM/genética
14.
Biomol Ther (Seoul) ; 32(2): 192-204, 2024 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-37551139

RESUMEN

Generally, odorant molecules are detected by olfactory receptors, which are specialized chemoreceptors expressed in olfactory neurons. Besides odorant molecules, certain volatile molecules can be inhaled through the respiratory tract, often leading to pathophysiological changes in the body. These inhaled molecules mediate cellular signaling through the activation of the Ca2+-permeable transient receptor potential (TRP) channels in peripheral tissues. This review provides a comprehensive overview of TRP channels that are involved in the detection and response to volatile molecules, including hazardous substances, anesthetics, plant-derived compounds, and pheromones. The review aims to shed light on the biological mechanisms underlying the sensing of inhaled volatile molecules. Therefore, this review will contribute to a better understanding of the roles of TRP channels in the response to inhaled molecules, providing insights into their implications for human health and disease.

15.
Sci Rep ; 14(1): 3011, 2024 02 06.
Artículo en Inglés | MEDLINE | ID: mdl-38321059

RESUMEN

Axillary odor is a malodor produced by bacterial metabolism near the apocrine glands, which often causes discomfort in an individual's daily life and social interactions. A deodorant is a personal care product designed to alleviate or mask body odor. Currently, most deodorants contain antimicrobial chemicals and fragrances for odor management; however, direct application to the underarm skin can result in irritation or sensitivity. Therefore, there is a growing interest in technologies that enable disinfection and odor control without the antiperspirants or perfumes. The cold atmospheric plasma temporally generates reactive radicals that can eliminate bacteria and surrounding odors. In this study, cultured Staphylococcus hominis and Corynebacterium xerosis, the causative bacteria of axillary bromhidrosis, were killed after 90% plasma exposure for 3 min. Moreover, the electronic nose system indicated a significant reduction of approximately 51% in 3-hydroxy-3-methylhexanoic acid and approximately 34% in 3-methyl-3-sulfanylhexan-1-ol, the primary components of axillary odor, following a 5-min plasma exposure. These results support the dual function of our deodorant in eliminating bacteria and axillary odors without the chemical agents. Therefore, cold atmospheric plasma-applied deodorant devices have great potential for the treatment and management of axillary odors as a non-contact approach without chemical use in daily life.


Asunto(s)
Desodorantes , Antibacterianos/farmacología , Odorantes , Piel , Glándulas Apocrinas , Bacterias , Axila/microbiología
16.
J Biol Chem ; 287(8): 6014-24, 2012 Feb 17.
Artículo en Inglés | MEDLINE | ID: mdl-22187434

RESUMEN

Spinal muscular atrophy and hereditary motor and sensory neuropathies are characterized by muscle weakness and atrophy caused by the degenerations of peripheral motor and sensory nerves. Recent advances in genetics have resulted in the identification of missense mutations in TRPV4 in patients with these hereditary neuropathies. Neurodegeneration caused by Ca(2+) overload due to the gain-of-function mutation of TRPV4 was suggested as the molecular mechanism for the neuropathies. Despite the importance of TRPV4 mutations in causing neuropathies, the precise role of TRPV4 in the sensory/motor neurons is unknown. Here, we report that TRPV4 mediates neurotrophic factor-derived neuritogenesis in developing peripheral neurons. TRPV4 was found to be highly expressed in sensory and spinal motor neurons in early development as well as in the adult, and the overexpression or chemical activation of TRPV4 was found to promote neuritogenesis in sensory neurons as well as PC12 cells, whereas its knockdown and pharmacologic inhibition had the opposite effect. More importantly, nerve growth factor or cAMP treatment up-regulated the expression of phospholipase A(2) and TRPV4. Neurotrophic factor-derived neuritogenesis appears to be regulated by the phospholipase A(2)-mediated TRPV4 pathway. These findings show that TRPV4 mediates neurotrophic factor-induced neuritogenesis in developing peripheral nerves. Because neurotrophic factors are essential for the maintenance of peripheral nerves, these findings suggest that aberrant TRPV4 activity may lead to some types of pathology of sensory and motor nerves.


Asunto(s)
Axones/metabolismo , Axones/patología , Neuropatía Hereditaria Motora y Sensorial/metabolismo , Neuropatía Hereditaria Motora y Sensorial/patología , Factores de Crecimiento Nervioso/metabolismo , Canales Catiónicos TRPV/metabolismo , Actinas/química , Animales , Ácido Araquidónico/farmacología , Axones/efectos de los fármacos , Adhesión Celular/efectos de los fármacos , Procesos de Crecimiento Celular/efectos de los fármacos , AMP Cíclico/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Técnicas de Silenciamiento del Gen , Humanos , Ratones , Neuritas/efectos de los fármacos , Neuritas/metabolismo , Neuritas/patología , Células PC12 , Nervios Periféricos/efectos de los fármacos , Nervios Periféricos/metabolismo , Nervios Periféricos/patología , Ésteres del Forbol/farmacología , Fosfolipasas A2/metabolismo , Multimerización de Proteína/efectos de los fármacos , Estructura Cuaternaria de Proteína , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Transducción de Señal/efectos de los fármacos , Canales Catiónicos TRPV/deficiencia , Canales Catiónicos TRPV/genética
17.
Mol Pain ; 9: 25, 2013 May 20.
Artículo en Inglés | MEDLINE | ID: mdl-23688027

RESUMEN

BACKGROUND: The quantification of pain intensity in vivo is essential for identifying the mechanisms of various types of pain or for evaluating the effects of different analgesics. A variety of behavioral tests for pain measurement have been devised, but many are limited because animals are physically restricted, which affects pain sensation. In this study, pain assessment was attempted with minimal physical restriction, and voluntary movements of unrestrained animals were used to evaluate the intensities of various types of pain. RESULTS: The number of times animals reared or total distances traveled was measured using a motion-tracking device and found to be markedly reduced in carrageenan-induced inflammatory, acetic acid-induced visceral, and streptozotocin-induced neuropathic pain tests. These two voluntary movement parameters were found to be highly correlated with paw withdrawal latency from irradiating heat. In addition, these parameters were markedly reversed by morphine and by non-steroidal anti-inflammatory drugs in inflammatory pain models. These parameters were also useful to detect hypoalgesia in TRPV1⁻/⁻ mice. CONCLUSIONS: These results suggest that parameters of voluntary movement, such as, number of rearing and total distance moved, are effective indicators of pain intensity for many types of pain and that they can be used to evaluate degree of pain perception.


Asunto(s)
Actividad Motora/fisiología , Dimensión del Dolor/normas , Analgésicos/farmacología , Analgésicos/uso terapéutico , Animales , Antiinflamatorios no Esteroideos/farmacología , Antiinflamatorios no Esteroideos/uso terapéutico , Carragenina/efectos adversos , Modelos Animales de Enfermedad , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Masculino , Ratones , Ratones Endogámicos , Neuralgia/inducido químicamente , Neuralgia/tratamiento farmacológico , Dolor , Dimensión del Dolor/métodos , Ratas , Canales Catiónicos TRPV/genética , Canales Catiónicos TRPV/metabolismo
18.
Pharmacology ; 92(3-4): 191-7, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24080610

RESUMEN

BACKGROUND AND PURPOSE: Poncirus fructus (PF), also known as the dried immature fruit of Poncirus trifoliata (L.) Raf., has long been used as a cure for the treatment of various gastrointestinal disorders in eastern Asia. Recently, it was reported that naringin, a flavonoid constituent of the PF extract, causes the activation of ghrelin receptor in vitro. Although the ghrelin receptor is involved in the enhancement of intestinal motility, there are no studies as yet involving in vivo action of naringin. Therefore, the purpose of the present study is to investigate whether naringin exhibits a prokinetic effect in vivo. METHODS: We measured the intestinal transit rate in rats with gastrointestinal motility dysfunction (GMD) and performed a pharmacokinetic analysis of naringin to investigate the effect of naringin on prokinetic activity in vivo. RESULTS: The results of this study show that the aqueous extract of PF and its constituent naringin have a strong prokinetic activity in GMD rats via activation of the ghrelin receptor. Surprisingly, pharmacokinetic analysis revealed that naringin has low bioavailability (11%), implying that the prokinetic effect of naringin was largely due to the local activation of ghrelin receptor in the intestine rather than a systemic effect after absorption. Indeed, it turned out that intravenous administration of naringin led to a lower prokinetic effect than when administrated orally to rats, indicating that naringin prefers to act on the intestinal wall rather than getting absorbed into the systemic circuit. This local mode of action might be advantageous for preventing possible systemic side effects since naringin is not well absorbed into the system circuit. CONCLUSIONS: Naringin exhibits an in vivo prokinetic activity by a preferable local activation of ghrelin receptor. Moreover, we propose that naringin could play a role as a leading compound for the development of ghrelin receptor-based prokinetic agents.


Asunto(s)
Flavanonas/farmacología , Motilidad Gastrointestinal/efectos de los fármacos , Receptores de Ghrelina/metabolismo , Animales , Flavanonas/sangre , Frutas , Absorción Intestinal/efectos de los fármacos , Masculino , Extractos Vegetales/farmacología , Poncirus , Ratas , Ratas Sprague-Dawley
19.
J Hazard Mater ; 455: 131555, 2023 08 05.
Artículo en Inglés | MEDLINE | ID: mdl-37156042

RESUMEN

Volatile organic compounds (VOCs) are gaseous chemicals found in ambient air and exhaled breath. In particular, highly reactive aldehydes are frequently found in polluted air and have been linked to various diseases. Thus, extensive studies have been carried out to elucidate disease-specific aldehydes released from the body to develop potential biomarkers for diagnostic purposes. Mammals possess innate sensory systems, such as receptors and ion channels, to detect these VOCs and maintain physiological homeostasis. Recently, electronic biosensors such as the electronic nose have been developed for disease diagnosis. This review aims to present an overview of natural sensory receptors that can detect reactive aldehydes, as well as electronic noses that have the potential to diagnose certain diseases. In this regard, this review focuses on eight aldehydes that are well-defined as biomarkers in human health and disease. It offers insights into the biological aspects and technological advances in detecting aldehyde-containing VOCs. Therefore, this review will aid in understanding the role of aldehyde-containing VOCs in human health and disease and the technological advances for improved diagnosis.


Asunto(s)
Receptores Odorantes , Compuestos Orgánicos Volátiles , Animales , Humanos , Odorantes , Nariz Electrónica , Aldehídos , Pruebas Respiratorias , Biomarcadores , Mamíferos
20.
J Mater Chem B ; 11(5): 1108-1114, 2023 02 01.
Artículo en Inglés | MEDLINE | ID: mdl-36629739

RESUMEN

The tissue-engineered vascular graft (TEVG) is a technology used to recreate a blood vessel by using vascular cells (endothelial cells and smooth muscle cells) and their scaffolds, and is a promising approach as a clinically feasible alternative for small-diameter blood vessel replacement. Since mechanical damage occurs during/after implantation, it needs flexibility and durability to withstand the mechanical damage to be applied. To achieve this, we applied a bioresorbable polyglycolic acid (PGA) fiber-knitted tubular scaffold for vascular endothelial and smooth muscle cell layers. Similar to the native rat aorta, the knitted tubular scaffold (130 µm-thick PGA fiber) exhibited mechanical performance at 150 mN for up to 40% strain for axial stress and at 90 mN for up to 5% strain for circumferential stress. After co-culturing, a vascular barrier comprised of an inner layer of endothelial cells and an outer layer of smooth muscle cells between tubular knits was observed. Up to 93.6% of the co-cultured cells were retained even after bending 50 times, and the suturability to flow liquid without any leakage in various shapes, such as an L-shape or a Y-shape, was acceptable. Taken together, these results support that the PGA tubular knit plays multifunctional roles, such as a porous three-dimensional matrix to attach and grow the vascular cells, and as a flexible and durable scaffold for the suture. Therefore, we suggest that the bioresorbable PGA tubular knit scaffold is a promising scaffold for TEVGs.


Asunto(s)
Prótesis Vascular , Ingeniería de Tejidos , Ratas , Animales , Ingeniería de Tejidos/métodos , Células Endoteliales/fisiología , Implantes Absorbibles , Técnicas de Cocultivo
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