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Many studies suggest that fear conditioning influences sleep. It is, however, not known if the changes in sleep architecture after fear conditioning are essentially associated with the consolidation of fearful memory or with fear itself. Here, we have observed that within sleep, NREM sleep consistently remained augmented after the consolidation of cued fear-conditioned memory. But a similar change did not occur after impairing memory consolidation by blocking new protein synthesis and glutamate transmission between glial-neuronal loop in the lateral amygdala (LA). Anisomycin (a protein synthesis inhibitor) and DL-α-amino-adipic acid (DL- α -AA) (a glial glutamine synthetase enzyme inhibitor) were microinjected into the LA soon after cued fear-conditioning to induce memory impairment. On the post-conditioning day, animals in both the groups exhibited significantly less freezing. In memory-consolidated groups (vehicle groups), NREM sleep significantly increased during 2nd to 5th hours after training compared to their baseline days. However, in memory impaired groups (anisomycin and DL- α -AA microinjected groups), similar changes were not observed. Our results thus suggest that changes in sleep architecture after cued fear-conditioning are indeed a consolidation dependent event.
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Condicionamiento Clásico/fisiología , Miedo , Consolidación de la Memoria/fisiología , Fases del Sueño , Animales , Anisomicina/administración & dosificación , Reacción de Prevención , Complejo Nuclear Basolateral/efectos de los fármacos , Masculino , Ratas Wistar , VigiliaRESUMEN
Specialized individual circuits in the brain are recruited for specific functions. Interestingly, multiple neural circuitries continuously compete with each other to acquire the specialized function. However, the dominant among them compete and become the central neural network for that particular function. For example, the hippocampal principal neural circuitries are the dominant networks among many which are involved in learning processes. But, in the event of damage to the principal circuitry, many times, less dominant networks compensate for the primary network. This review highlights the psychopathologies of functional loss and the aspects of functional recuperation in the absence of the hippocampus.
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The dorsal hippocampus (DH) is primarily involved in the formation of contextual fear-conditioned (CxFC) memory. However, CxFC memory can be formed even in the absence of the DH. In addition to the DH, the infralimbic cortex (IL), a sub-region of the medial prefrontal cortex (mPFC), also plays an important role in the consolidation of CxFC memory. However, role of IL in the development of compensatory CxFC memory is not known. Here, we have examined (a) the development of the compensatory circuitry of CxFC memory within 3 days after the first test in the absence of the DH and (b) the role of IL in the induction of compensatory CxFC memory in the absence of the DH. The DH-lesioned rats re-trained for CxFC 1 day after the first testing exhibited significantly less freezing compared to the control group. However, the DH-lesioned rats, re-trained for CxFC 3 days after the first testing, showed a robust freezing response. It suggests that the fully functional compensatory circuitry of contextual fear memory develops after multiple training separated by 3 days. Furthermore, we observed that reversible inactivation of the IL of the DH-lesioned rats during the first training waned the formation of compensatory CxFC. It suggests that (a) the IL receives contextual fear memory information during the first trial in the absence of the DH and (b) perturbation in fear memory information encoding in the IL during the first trial impairs the development of the compensatory network in the absence of the DH.
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Miedo , Hipocampo , Ratas , Animales , Ratas Wistar , Hipocampo/fisiología , Miedo/fisiología , Corteza Prefrontal/metabolismoRESUMEN
The hippocampus-dependent "trace-appetitive conditioning task" increases cell proliferation and the generation of newborn young neurons. Evidence suggests that adult hippocampal neurogenesis and rapid eye movement (REM) sleep play an essential role in memory consolidation. On the other hand, REM sleep deprivation (REM-SD) induces detrimental effects on training-induced cell proliferation in the hippocampus's dentate gyrus (DG). Nonetheless, the role of REM sleep in the trace-appetitive memory and fate determination of the newly proliferated cells is not known. Here, we have studied the following: (I) the effects of 24 h of REM-SD (soon after training) on trace- and delay-appetitive memory and cell proliferation in the adult DG and (II) the effects of chronic (96 h) REM-SD (3 days after the training, the period in which newly generated cells progressed toward the neuronal lineage) on trace-appetitive memory and the generation of newborn young neurons. We used a modified multiple platform method for the selective REM-SD without altering non-REM (NREM) sleep. We found that 24 h of REM-SD, soon after trace-conditioning, impaired the trace-appetitive memory and the training-induced cell proliferation. Nevertheless, 96 h of REM-SD (3 days after the training) did not impair trace memory. Interestingly, 96 h of REM-SD altered the generation of newborn young neurons. These results suggest that REM sleep plays an essential role in training-induced cell proliferation and the fate determination of the newly generated cells toward the neuronal lineage.
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Giro Dentado , Privación de Sueño , Proliferación Celular , Hipocampo , Humanos , NeuronasRESUMEN
Sleep may help consolidate the information of certain memories, though its benefits in the consolidation of trace-conditioned memory still remain elusive. We investigated the effect of sleep deprivation on trace learning in male wistar rats. Rats were trained for trace conditioning and the number of head entries into liquid dispenser was accounted as an outcome measure of trace-learning. For training and testing, 75 presentations of conditioned stimulus (CS) (light) and unconditioned stimulus (US) (juice) were offered in five sessions (15 presentations/session; with 5 min inter-session gap). The duration of CS and US stimuli were 15 and 20s respectively, with 5s trace delay between stimuli and 20s condition delay between each presentation. The animals were divided randomly into three groups soon after training, sleep deprived (SD) (n=8), non-SD (NSD) (n=8) and stress control (n=5) groups. The animals of NSD and control groups were left undisturbed, while SD animals were sleep deprived for 6h after training. The learning of trace-conditioned task was examined on following days. We observed that SD rats poked approximately 63% less than NSD and control groups (p<0.001) to obtain juice on testing day. Also, the NSD rats exhibited significant positive correlation in number of head entries during the training and testing days; while the SD rats showed no significant correlation. The results demonstrate that SD animals had difficulties to associate CS with US and suggest that sleep deprivation soon after training impairs the encoding of trace memory.
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Condicionamiento Clásico/fisiología , Memoria/fisiología , Privación de Sueño/fisiopatología , Animales , Aprendizaje por Asociación/fisiología , Masculino , Distribución Aleatoria , Ratas , Ratas WistarRESUMEN
Alteration of the bodily CO2 concentration and proton pump activity affects the sleep architecture. The brainstem locus coeruleus (LC) area plays an essential role in rapid eye movement (REM) sleep generation and chemoregulation. Previously, we reported that lansoprazole injections (intraperitoneal) increased REM sleep in the rats. However, it is not known if proton pumps in the LC influence REM sleep. Here, we studied the effects of lansoprazole in the LC on the neuronal activity and REM sleep expression. Male Wistar rats (250-300 g) were surgically prepared for sleep recording and drug microinjections into the LC. We determined the localization of proton pumps and expression levels of cFOS in the LC neurons immunohistochemically. Sleep-wake was recorded before and after the microinjections of drugs/vehicles. Our results demonstrate (i) the presence of proton pumps in the LC neurons, (ii) that the microinjection of lansoprazole into the LC reduced the number of cFOS+ve-TH+ve double-labeled neurons in the LC by 52.6% (p < 0.001) compared to the vehicle and (iii) that low and high doses of lansoprazole significantly increased REM sleep by 32% (p < 0.001) and 60% (p < 0.001), respectively, compared to the vehicle. Our results suggest that the proton pumps modulate the LC's noradrenergic (NE-ergic) neuronal activity and REM sleep. The increased amount of REM sleep can be attributed to the inhibition of the LC NE-ergic activity. Further, the REM sleep amount increased after the lansoprazole microinjections into the LC with a significant increase in the REM sleep episode numbers. Overall, our results suggest that proton pumps in the LC may be involved in REM sleep generation.
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Locus Coeruleus , Sueño REM , Animales , Electroencefalografía , Lansoprazol/farmacología , Masculino , Microinyecciones , Neuronas , Inhibidores de la Bomba de Protones/farmacología , Ratas , Ratas Wistar , Sueño , VigiliaRESUMEN
The locus coeruleus (LC) is one of the essential chemoregulatory and sleep-wake (S-W) modulating centers in the brain. LC neurons remain highly active during wakefulness, and some implicitly become silent during rapid eye movement (REM) sleep. LC neurons are also involved in CO2-dependent modulation of the respiratory drive. Acid-sensing ion channels (ASICs) are highly expressed in some brainstem chemosensory breathing regulatory areas, but their localization and functions in the LC remain unknown. Mild hypercapnia increases the amount of non-REM (NREM) sleep and the number of REM sleep episodes, but whether ASICs in the LC modulate S-W is unclear. Here, we investigated the presence of ASICs in the LC and their role in S-W modulation and the state transition from NREM to REM sleep. Male Wistar rats were surgically prepared for chronic polysomnographic recordings and drug microinjections into the LC. The presence of ASIC-2 and ASIC-3 in the LC was immunohistochemically characterized. Microinjections of amiloride (an ASIC blocker) and APETx2 (a blocker of ASIC-2 and -3) into the LC significantly decreased wakefulness and REM sleep, but significantly increased NREM sleep. Mild hypercapnia increased the amount of NREM and the number of REM episodes. However, APETx2 microinjection inhibited this increase in REM frequency. These results suggest that the ASICs of LC neurons modulate S-W, indicating that ASICs could play an important role in vigilance-state transition. A mild increase in CO2 level during NREM sleep sensed by ASICs could be one of the determinants of state transition from NREM to REM sleep.
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Sueño REM , Vigilia , Canales Iónicos Sensibles al Ácido , Animales , Electroencefalografía , Locus Coeruleus , Masculino , Ratas , Ratas Wistar , SueñoRESUMEN
Among conventional cancer therapies, radio-frequency magnetic hyperthermia (MHT) has widely been investigated for use with magnetic nanoparticles (MNPs). However, the majority of in vivo biodistribution studies have tested very low MNP dosages (equivalent to magnetic resonance imaging (MRI) applications) to check for clearance rate; which is far below the clinical dose of MHT. Due to this poor validation in preclinical scenarios, quite a few MNPs already in clinical use were later discontinued, on grounds of unexpected clinical outcomes in terms of inflammation, and prolonged clearance in vivo. By exploiting an economical method of synthesis, we have developed chitosan-coated Fe3O4 nanoparticles with high heating efficiency performance. Their anti-tumor response was evaluated in an ectopic tumor model of C6 glioblastoma by MHT. The intratumoral injection of MNPs on days 1 and 7 resulted in rapid tumor inhibition rate of 69.4% within 8 days, with complete inhibition within 32 days, and no recurrence recorded over a 5-month follow-up. Notably, the MNP-mediated MHT therapy achieved the highest degree of therapeutic efficacy required for complete tumor ablation by combining controlled temperature range (<44 °C), reduced MNP dosage; much lower than in most reported studies, and AMF parameters (time of exposure and frequency) within the clinical safety limit. Periodic body weight measurements confirmed negligible adverse side effects in rats. The anti-tumor activity was validated by severe apoptosis (TUNEL, cleaved Caspase-3), reduced proliferation (Ki 67) and disrupted vasculature (CD 31) in the Fe3O4-MHT-treated group. Real-time gene expression of pro-inflammatory cytokines (IL-6, TNF-α, IL-1α, IL-1ß) confirmed the intratumoral activation of IL-6, suggesting the role of immunomodulation in triggering the adaptive immune response for faster tumor regression in the treated group. In addition, the biodistribution and clearance rate of MNPs monitored using ICP-OES confirmed their time-dependent biodegradation via excretion (urine, feces), phagocytosis (liver) and circulatory system (blood), with negligible deposition in other major organs (kidney, heart, lungs). Although we could not show complete clearance of our MNPs within the time frame tested, future studies should focus on combining MHT with immunotherapy, and target tumors at a much-reduced iron dose, consequently improving in vivo clearance rate, and hence overcoming the limitations of MHT in clinical therapy.
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Hipertermia Inducida , Nanopartículas de Magnetita , Animales , Caspasa 3 , Línea Celular Tumoral , Compuestos Férricos , Glioblastoma , Nanopartículas Magnéticas de Óxido de Hierro , Ratas , Distribución TisularRESUMEN
Adult hippocampal neurogenesis (AHN) plays an essential role in hippocampal-dependent memory consolidation. Increased neurogenesis enhances learning, whereas its ablation causes memory impairment. In contrast, few reports suggest that neurogenesis reduces after learning. Although the interest in exploring the role of adult neurogenesis in learning has been growing, the evidence is still limited. The role of the trace- and delay-appetitive-conditioning on AHN and its underlying mechanism are not known. The consolidation of trace-conditioned memory requires the hippocampus, but delay-conditioning does not. Moreover, the dorsal hippocampus (DH) and ventral hippocampus (VH) may have a differential role in these two conditioning paradigms. Here, we have investigated the changes in: (A) hippocampal cell proliferation and their progression towards neuronal lineage; and (B) expression of Arc, Erk1, Erk2, and CREB proteins in the DH and VH after trace- and delay-conditioning in the rat. The number of newly generated cells significantly increased in the trace-conditioned but did not change in the delay-conditioned animals compared to the control group. Similarly, the expression of Arc protein significantly increased in the DH but not in the VH after trace-conditioning. Nonetheless, it remains unaltered in the delay-conditioned group. The expression of pErk1, pErk2, and pCREB also increased in the DH after trace-conditioning. Whereas, the expression of only pErk1 pErk2 and pCREB proteins increased in the VH after delay-conditioning. Our results suggest that appetitive trace-conditioning enhances AHN. The increased DH neuronal activation and pErk1, pErk2, and pCREB in the DH may be playing an essential role in learning-induced cell-proliferation after appetitive trace-conditioning.
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Aim: To explore the optimum temperature for cancer cell death using magnetic hyperthermia (MH), which in turn will affect the mode of cell death. Method: The focus of this study is to improve upon the existing methodology for the synthesis of chitosan encapsulated Fe3O4. MH was done at different temperatures. The cell death pathway was explored using flow cytometry and western blot. Results: Coated Fe3O4 exhibited low cytotoxicity, high stability and heating efficiency. MH at 43°C was the optimum temperature for robust cell death. Cell death pathway suggested that during the initial stages of recovery, apoptosis was the main mode of cell death. While at later stages, major apoptosis and minor necrosis were observed. Conclusion: It is important to find out the long-term effect of hyperthermia treatment on cancer cells and their consequences on surrounding healthy cells.
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Caspasa 3/metabolismo , Compuestos Férricos/química , Hipertermia Inducida/métodos , Nanopartículas de Magnetita/química , Apoptosis , Western Blotting , Citometría de Flujo , Humanos , TemperaturaRESUMEN
Although the dorsal hippocampus (DH) plays an essential role in the consolidation of contextual fear-conditioned (CxFC) memory, this consolidation may also occur in the absence of DH. It is, however, not known if the development of a compensatory circuit for CxFC memory is time-dependent. The DH-dependent contextual fear memory influences sleep architecture, but whether the compensatory fear memory can influence sleep, is not known. Here, we have studied (a) the temporal progression of compensatory contextual fear memory in the absence of DH and (b) the influence of compensatory contextual fear memory on sleep architecture. Rats were surgically prepared for chronic polysomnographic recordings and drug injections in the DH. They were divided into four groups: DH-non-lesioned and fear-conditioned, DH-non-lesioned and non-fear-conditioned, DH-lesioned and fear-conditioned and DH-lesioned and non-fear-conditioned groups. The DH was lesioned with ibotenic acid. The animals were conditioned to contextual fear twice: 1st training on Day 5 and testing on Day 6; 2nd training on Day 10 and testing on Day 11. The DH-lesioned and fear-conditioned animals did not exhibit freezing response during the first testing but showed a robust freezing response when re-trained after a gap of three days. In addition, wakefulness and NREM sleep amount did not change, but REM sleep significantly decreased in the DH-dependent CxFC memory group. Interestingly, REM sleep did not decrease in the DH-independent CxFC memory group. Our findings suggest that the development of compensatory CxFC memory is a time-dependent process and the compensatory CxFC memory may not influence sleep architecture.
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Miedo/fisiología , Memoria/fisiología , Sueño/fisiología , Animales , Condicionamiento Clásico/fisiología , Condicionamiento Psicológico/fisiología , Hipocampo/fisiología , Ácido Iboténico/farmacología , Aprendizaje/fisiología , Masculino , Consolidación de la Memoria/fisiología , Ratas , Ratas Wistar , Sueño REM/fisiología , Vigilia/fisiologíaRESUMEN
STUDY OBJECTIVES: The effects of hypnotics on sleep-dependent brain plasticity are unknown. We have shown that sleep enhances a canonical model of in vivo cortical plasticity, known as ocular dominance plasticity (ODP). We investigated the effects of 3 different classes of hypnotics on ODP. DESIGN: Polysomnographic recordings were performed during the entire experiment (20 h). After a baseline sleep/wake recording (6 h), cats received 6 h of monocular deprivation (MD) followed by an i.p. injection of triazolam (1-10 mg/kg i.p.), zolpidem (10 mg/kg i.p.), ramelteon (0.1-1 mg/kg i.p.), or vehicle (DMSO i.p.). They were then allowed to sleep ad lib for 8 h, after which they were prepared for optical imaging of intrinsic cortical signals and single-unit electrophysiology. SETTING: Basic neurophysiology laboratory PATIENTS OR PARTICIPANTS: Cats (male and female) in the critical period of visual development (postnatal days 28-41) INTERVENTIONS: N/A. MEASUREMENTS AND RESULTS: Zolpidem reduced cortical plasticity by approximately 50% as assessed with optical imaging of intrinsic cortical signals. This was not due to abnormal sleep architecture because triazolam, which perturbed sleep architecture and sleep EEGs more profoundly than zolpidem, had no effect on plasticity. Ramelteon minimally altered sleep and had no effect on ODP. CONCLUSIONS: Our findings demonstrate that alterations in sleep architecture do not necessarily lead to impairments in sleep function. Conversely, hypnotics that produce more "physiological" sleep based on polysomnography may impair critical brain processes, depending on their pharmacology.
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Corteza Cerebral/efectos de los fármacos , Hipnóticos y Sedantes/farmacología , Plasticidad Neuronal/efectos de los fármacos , Piridinas/farmacología , Sueño/efectos de los fármacos , Animales , Mapeo Encefálico , Gatos , Dominancia Cerebral/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Electroencefalografía/efectos de los fármacos , Potenciales Evocados Visuales/efectos de los fármacos , Femenino , Indenos/farmacología , Inyecciones Intraperitoneales , Masculino , Polisomnografía/efectos de los fármacos , Procesamiento de Señales Asistido por Computador , Fases del Sueño/efectos de los fármacos , Tomografía de Coherencia Óptica , Triazolam/farmacología , ZolpidemRESUMEN
We investigated sleep ontogenesis in the ferret-a placental mammal that is highly altricial compared to other mammalian species. Because altriciality is linked with elevated rapid-eye-movement (REM) sleep amounts during infancy, it was expected that ferret kits would display very high levels of this state. Longitudinal polysomnographic measurements were made from 8 ferret kits from approximately eye-opening (postnatal day [P]30)-P50 using an experimental routine that minimized the effects of maternal separation. These data were compared to values from 8 adult ferrets (>3 months of age) and 6 neonatal cats (mean age: P31.7). We find that the polygraphic features of REM and non-REM (NREM) sleep are present by at least P30. Over the next 2 weeks, REM sleep amounts slightly declined while wakefulness and NREM sleep amounts increased. However, a comparison to published values from developing cats and rats showed that the ferret did not exhibit a disproportionate amount of REM sleep at similar postnatal ages or relative to a common developmental milestone (eye-opening).
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Animales Recién Nacidos/fisiología , Encéfalo/fisiología , Hurones/fisiología , Fases del Sueño/fisiología , Vigilia/fisiología , Animales , Encéfalo/crecimiento & desarrollo , Gatos , Femenino , Hurones/crecimiento & desarrollo , Masculino , Proyectos Piloto , PolisomnografíaRESUMEN
Oscillating waves during sleep play an essential role in memory consolidation. The cortical slow wave activity (SWA) and sigma waves during NREM sleep and theta waves during REM sleep increase after a variety of memory tasks including declarative, procedural and associative learning tasks. These oscillatory waves during sleep help to promote neural dialog between circuitries, which possibly plays a causal role in memory consolidation. However, the role of sleep-associated oscillating waves in a complex appetitive-conditioning paradigm is not clear. The parietal cortex and amygdala are involved in the cognitive evaluation of the environmental stimuli, and appetitive conditioning. Here, we have studied the changes in sleep architecture and oscillatory waves during NREM and REM sleep in the parietal cortices and amygdalar-local field potential (A-LFP) after appetitive-conditioning in the rat. We observed that REM sleep increased significantly after appetitive conditioning, which significantly positively correlated with performance on the appetitive-conditioning task. Further, the cortical SWA (0.1-4.5 Hz), and sigma (12-14.25 Hz) waves during NREM sleep, theta (6-9 Hz) waves during REM sleep, the amygdalar SWA (0.1-3.75 Hz) during NREM sleep and theta (6-8.25 Hz) waves during REM sleep significantly increased after appetitive conditioning. Interestingly, the augmented oscillatory waves significantly positively correlated with the performances on the appetitive-conditioning task. Our results suggest that the augmented REM sleep after conditioning may be required for the consolidation of appetitive-conditioned memory. Further, a significant correlation between augmented power in oscillatory waves during sleep and performance suggesting that these waves may be playing a crucial role in the consolidation of appetitive-conditioned memory.
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We have produced an innovative, theranostic hybrid nanocomposite of graphene oxide and iron oxide (GO-Fe3O4) for radio-frequency hyperthermia therapy. A new electrochemical synthesis route for the GO-Fe3O4 nanocomposite is employed. Superparamagnetic nanoparticles used for magnetic hyperthermia for biomedical application face longstanding obstacles, including the large number of nanoparticles required to achieve the desired therapeutic temperature, poor colloidal stability in aqueous suspension or physiological media, poor biocompatibility and, most importantly, low specific absorption rate (SAR). To limit the dosage of nanoparticles for therapeutic use, efforts are being made to increase the heating efficiency of nanoparticles. We have introduced an alternative way to increase the SAR value by improving the colloidal stability of magnetic nanoparticles. It is necessary to immobilize these nanoparticles on a support to prevent their agglomeration and precipitation in aqueous suspension. To address these issues, we report a reproducible electrochemical synthesis route for the GO-Fe3O4 nanocomposite. Our nanocomposite demonstrated good colloidal stability and low cytotoxicity in vitro. Due to its good colloidal stability, the nanocomposite had a high SAR of 543 W g-1 and corresponding intrinsic loss power of 5.98 nH m2 kg-1, which is 46% better than the best commercial equivalents. In vitro cytotoxicity studies demonstrated almost 70% cell viability at 200 µg mL-1 GO-Fe3O4 nanocomposite, a comparable concentration for clinical use according to FDA standards. We also showed the therapeutic potential of the nanocomposite using magnetic hyperthermia. We observed cancer cell (A549 human lung epithelial adenocarcinoma) ablation at 41, 42 and 43 °C for 30, 45, and 60 min. A maximum cancer cell death rate of 80.5% was observed at 43 °C for 60 min under alternating magnetic field exposure. Thus, the nanocomposites could be used in the efficient treatment of cancer.
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The conditioning tasks have been widely used to model fear and anxiety and to study their association with sleep. Many reports suggest that sleep plays a vital role in the consolidation of fear memory. Studies have also demonstrated that fear-conditioning influences sleep differently in mice strains having a low or high anxiety level. It is, therefore, necessary to know, how sleep influences fear-conditioning and how fear-conditioning induces changes in sleep architecture in moderate anxious strains. We have used Swiss mice, a moderate anxious strain, to study the effects of: (i) sleep deprivation on contextual fear conditioned memory, and also (ii) contextual fear conditioning on sleep architecture. Animals were divided into three groups: (a) non-sleep deprived (NSD); (b) stress control (SC); and (c) sleep-deprived (SD) groups. The SD animals were SD for 5 h soon after training. We found that the NSD and SC animals showed 60.57% and 58.12% freezing on the testing day, while SD animals showed significantly less freezing (17.13% only; p < 0.001) on the testing day. Further, we observed that contextual fear-conditioning did not alter the total amount of wakefulness and non-rapid eye movement (NREM) sleep. REM sleep, however, significantly decreased in NSD and SC animals on the training and testing days. Interestingly, REM sleep did not decrease in the SD animals on the testing day. Our results suggest that short-term sleep deprivation impairs fear memory in moderate anxious mice. It also suggests that NREM sleep, but not REM sleep, may have an obligatory role in memory consolidation.
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Recent findings in humans and animals suggest that sleep promotes synaptic plasticity, but the underlying mechanisms have not been identified. We have demonstrated recently an important role for sleep in ocular dominance (OD) plasticity, a classic form of in vivo cortical remodeling triggered by monocular deprivation (MD) during a critical period of development. The mechanisms responsible for the effects of sleep on OD plasticity are unknown but may depend on neuronal activity in the sleeping brain. We investigated the role of cortical activity in sleep-dependent plasticity by reversibly inactivating the sleeping visual cortex (V1) after a period of MD. Critical period cats were bilaterally implanted with cannulas in V1 and standard EEG/EMG electrodes for polysomnographic recording. After a period of MD, visual cortices were infused with the sodium channel blocker lidocaine in vehicle or vehicle only during sleep. A third group of cats served as sham controls and were infused with lidocaine outside of V1 (into the CSF). Both optical imaging of intrinsic cortical signals and microelectrode recordings showed that OD plasticity was significantly reduced in cats whose visual cortices were reversibly silenced during sleep. These findings demonstrate that the mechanisms governing this form of sleep-dependent plasticity require cortical activity. They provide an important insight into how sleep modifies synaptic circuitry by narrowing the range of possible candidate mechanisms to those that are activity dependent.
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Predominio Ocular , Plasticidad Neuronal/fisiología , Sueño/fisiología , Corteza Visual/fisiología , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Análisis de Varianza , Anestésicos Locales/farmacología , Animales , Animales Recién Nacidos , Conducta Animal , Mapeo Encefálico , Gatos , Período Crítico Psicológico , Diagnóstico por Imagen/métodos , Electroencefalografía/métodos , Electromiografía/métodos , Lateralidad Funcional/fisiología , Técnicas In Vitro , Lidocaína/farmacología , Plasticidad Neuronal/efectos de los fármacos , Privación Sensorial/fisiología , Corteza Visual/citología , Corteza Visual/efectos de los fármacos , Vías Visuales/efectos de los fármacos , Vías Visuales/fisiologíaRESUMEN
Ocular dominance plasticity is enhanced by sleep and reduced by sleep deprivation or when all neural activity in the sleeping visual cortex is reversibly inhibited. These latter findings demonstrate that the mechanisms responsible for the effects of sleep on cortical plasticity are activity dependent. To better isolate this activity dependent mechanism, we investigated the role of postsynaptic activity by inactivating the sleeping visual cortex after a period of monocular deprivation with the gamma-amino-n-butyric acid agonist muscimol. Microelectrode recordings showed that ocular dominance plasticity was significantly reduced in cortices reversibly silenced during sleep compared with cortices infused with vehicle only. These findings demonstrate that postsynaptic activity during sleep is required for the consolidation of experience-dependent cortical plasticity.
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Inhibición Neural/fisiología , Plasticidad Neuronal/fisiología , Sueño/fisiología , Sinapsis/fisiología , Corteza Visual/fisiología , Vías Visuales/fisiología , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Animales , Gatos , Predominio Ocular/fisiología , Electroencefalografía , Electromiografía/métodos , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Agonistas del GABA/farmacología , Muscimol/farmacología , Inhibición Neural/efectos de los fármacos , Plasticidad Neuronal/efectos de los fármacos , Sueño/efectos de los fármacos , Privación de Sueño/fisiopatología , Sinapsis/efectos de los fármacos , Corteza Visual/efectos de los fármacos , Vías Visuales/efectos de los fármacosRESUMEN
We investigated sleep-wake (S-W) architecture and sleep regulation in the ferret: a phylogenetically primitive mammal increasingly used in neurobiological studies. Twenty-four hour S-W baseline data were collected in eight adult ferrets. Seven ferrets were then sleep deprived for 6h at the beginning of the light period. Like other placental mammals, ferrets exhibited the main vigilance states of wakefulness, rapid-eye-movement (REM) sleep and non-REM (NREM) sleep. Interestingly, the amount of REM sleep in the ferret was considerably higher (24.01+/-1.61% of total recording time) than typically reported in placental mammals. Ferret sleep was homeostatically regulated as sleep deprivation produced a significant increase in NREM EEG delta power during the recovery period. Therefore, ferret sleep in most respects is comparable to sleep in other placental mammals. However, the large amount of REM sleep in this phylogenetically more ancient species suggests that REM sleep may have been present in greater amounts in early stages of mammalian evolution.