RESUMEN
In this work, the waste orange-peel was used as carbon source, and the orange-peel derived carbon material can be obtained through simple pyrolysis. Then, we designed the structure of orange-peel carbon aerogel grown on iron-nickel layered double hydroxides in situ to achieve the effect of carbon coating (FeNi-LDH/CA). The oxygen evolution reaction catalytic performance of FeNi-LDH/CA is excellent, far exceeding that of commercial RuO2. In 1 M KOH, the overpotential of FeNi-LDH/CA is only 250 mV (10 mA cm-2), obviously better than that of commercial RuO2 (295 mV). FeNi-LDH/CA shows good cycling stability, and after long-term i-t testing, the performance only decays by 3% after running at 100 mA cm-2 for 100 h. When used as an anode, the voltage of water-splitting is only 1.48 V at 10 mA cm-2. The rechargeable liquid zinc-air battery based on Pt/C-FeNi-LDH/CA catalyst has higher open-circuit voltage (1.543 V) and galvanostatic discharge capacity at 1.23 V (830 min, 10 mA cm-2). Moreover, the zinc-air battery based on Pt/C-FeNi-LDH/CA has a small charge-discharge voltage gap (0.65 V) at 10 mA cm-2, after 200 consecutive cycles (66 h), the charge-discharge voltage gap only increased by about 30 mV, indicating good cycling stability.
RESUMEN
OBJECTIVE: To explore the potential of metanephric mesenchymal cells (MMCs) for osteogenesis and naringin's ability to enhance this process and its molecular mechanism. METHODS: Porcine MMCs at 70 days of gestation were used as tool cells, cultured in osteogenic induction medium, identified by immunocytochemistry staining. Osteogenic potential of porcine MMCs and naringin's ability to enhance this process was tested by detecting changes in cell viability, alkaline phosphatase (ALP) activity, the expression of runt-related transcription factor 2 (Runx2), osteopontin (OPN) and osteocalcin (OCN), and the formation of mineralized nodules, and the application of the p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin. RESULTS: Immunocytochemical staining showed that the cells were Vimentin and Six2(+), E-cadherin and CK-18(-). Naringin can activate the p38 signaling pathway to enhance the osteogenesis of porcine MMCs by increasing cell viability, ALP activity, the expressions of Runx2, OPN and OCN, and the formation of mineralized nodules (P<0.05). The application of p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin, manifested by decreased ALP activity, the expressions of Runx2, OPN and OCN, and the formation of mineralized nodules (P<0.05). CONCLUSION: Naringin, the active ingredient of Chinese herbal medicine Rhizoma Drynariae for nourishing Shen (Kidney) and strengthening bone, enhances the osteogenic differentiation of renal MMCs through the p38 signaling pathway.