Domestic cat nose functions as a highly efficient coiled parallel gas chromatograph.

The peripheral structures of mammalian sensory organs often serve to support their functionality, such as alignment of hair cells to the mechanical properties of the inner ear. Here, we examined the structure-function relationship for mammalian olfaction by creating an anatomically accurate computational nasal model for the domestic cat (Felis catus) based on high resolution microCT and sequential histological sections. Our results showed a distinct separation of respiratory and olfactory flow regimes, featuring a high-speed dorsal medial stream that increases odor delivery speed and efficiency to the ethmoid olfactory region without compromising the filtration and conditioning purpose of the nose. These results corroborated previous findings in other mammalian species, which implicates a common theme to deal with the physical size limitation of the head that confines the nasal airway from increasing in length infinitely as a straight tube. We thus hypothesized that these ethmoid olfactory channels function as parallel coiled chromatograph channels, and further showed that the theoretical plate number, a widely-used indicator of gas chromatograph efficiency, is more than 100 times higher in the cat nose than an "amphibian-like" straight channel fitting the similar skull space, at restful breathing state. The parallel feature also reduces airflow speed within each coil, which is critical to achieve the high plate number, while feeding collectively from the high-speed dorsal medial stream so that total odor sampling speed is not sacrificed. The occurrence of ethmoid turbinates is an important step in the evolution of mammalian species that correlates to their expansive olfactory function and brain development. Our findings reveal novel mechanisms on how such structure may facilitate better olfactory performance, furthering our understanding of the successful adaptation of mammalian species, including F. catus, a popular pet, to diverse environments.

Nasal Structural and Aerodynamic Features That May Benefit Normal Olfactory Sensitivity.

Nasal airflow that effectively transports ambient odors to the olfactory receptors is important for human olfaction. Yet, the impact of nasal anatomical variations on airflow pattern and olfactory function is not fully understood. In this study, 22 healthy volunteers were recruited and underwent computed tomographic scans for computational simulations of nasal airflow patterns. Unilateral odor detection thresholds (ODT) to l-carvone, phenylethyl alcohol (PEA) and d-limonene were also obtained for all participants. Significant normative variations in both nasal anatomy and aerodynamics were found. The most prominent was the formation of an anterior dorsal airflow vortex in some but not all subjects, with the vortex size being significantly correlated with ODT of l-carvone (r = 0.31, P < 0.05). The formation of the vortex is likely the result of anterior nasal morphology, with the vortex size varying significantly with the nasal index (ratio of the width and height of external nose, r = -0.59, P < 0.001) and nasal vestibule "notch" index (r = 0.76, P < 0.001). The "notch" is a narrowing of the upper nasal vestibule cartilage region. The degree of the notch also significantly correlates with ODT for PEA (r = 0.32, P < 0.05) and l-carvone (r = 0.33, P < 0.05). ODT of d-limonene, a low mucosal soluble odor, does not correlate with any of the anatomical or aerodynamic variables. The current study revealed that nasal anatomy and aerodynamics might have a significant impact on normal olfactory sensitivity, with greater airflow vortex and a narrower vestibule region likely intensifying the airflow vortex toward the olfactory region and resulting in greater olfactory sensitivity to high mucosal soluble odors.

Tuning to odor solubility and sorption pattern in olfactory epithelial responses.

Odor information is first represented as a spatial activation pattern across the olfactory epithelium, when odor is drawn into the nose through breathing. This epithelial pattern likely results from both the intrinsic olfactory sensory neuron (OSN) sensitivity and the sorptive patterns imposed by the interaction of nasal aerodynamics with physiochemical properties of odorants, although the precise contributions of each are ill defined. Here, we used a computational fluid dynamics (CFD) model of rat nasal cavity to simulate the nasal aerodynamics and sorption patterns for a large number of odorants, and compared the results with the spatial neural activities measured by electro-olfactogram (EOG) under same flow conditions. The computational and experimental results both indicate greater sorption and response to a narrow range odorants as a function of their mucosal solubility, and this range can be further modulated by changes of intranasal flow rates and direction (orthonasal vs retronasal flow). A striking finding is that the profile of intrinsic EOG response measured in surgically opened nose without airflow constraints is similar to the shape of the sorption profile imposed by nasal airflow, strongly indicating a tuning process. As validation, combining the intrinsic response with the mucosal concentration estimated by CFD in nonlinear regression successfully accounts for the measured retronasal and orthonasal EOG response at all flow rates and positions. These observations redefine the role of sorption properties in olfaction and suggest that the peripheral olfactory system, especially the central zone, may be strategically arranged spatially to optimize its functionality, depending on the incoming stimuli.

Self-assembly of nano/micro-structured Fe3O4 microspheres among 3D rGO/CNTs hierarchical networks with superior lithium storage performances.

Nano/micro-structured Fe3O4 microspheres among three-dimensional (3D) reduced graphene oxide (rGO)/carbon nanotubes (CNTs) hierarchical networks (the ternary composite is denoted as rGCFs) have been synthesized using a facile, self-assembled and one-pot hydrothermal approach. The rGCFs composite exhibits superior lithium storage performances: initial discharge and charge capacities of 1452 and 1036 mAh g(-1), respectively, remarkable rate capability at current densities from 100 mA g(-1) to 10 A g(-1) and outstanding cycling performance up to 200 cycles. The highly enhanced electrochemical performances of rGCFs depend heavily on the robust 3D rGO/CNTs hierarchical networks, the stable nano/microstructures of active Fe3O4 microspheres and the positive synergistic effects of building components. The systematic structure characterizations and electrochemical investigations provide insightful understanding towards the relationship between structure/morphology and lithium storage performances, which may pave the way for the rational design of composite materials with desirable goals.

[Research on pretreatment and envelope extraction algorithm of heart sound signal].

In this work, a new method of heart sound signal preprocessing is presented. First, the heart sound signals are decomposed by using multilayer wavelet transform. And then double parameters as thresholds are used in processing each layer after decomposition for denoising. Next, reconstruction of heart sound signals could be done after processing last layer. Four methods, i.e. wavelet transform, Hilbert-Huang transform (HHT), mathematical morphology, and normalized average Shannon energy, were used to extract the envelop of the heart sound signals respectively after reconstruction of heart sounds. All methods were improved in this study. We finally in our study chose 30 cases of raw heart sound signals, which were selected randomly from a database comed from The Clinical Medicine Institute of Montreal, and processed them by using the improved methods. The results were satisfactory. It showed that the extracted envelope with the original signal has a high degree of matching, whether it is a low frequency portion or high frequency portion. Most of all information of heart sound has been maintained in the envelope.

Abnormal Changes in IL-13 and IL-17A Serum Levels in Children with Adenovirus Pneumonia and their Diagnostic Value.

Background: Human adenovirus (HAdV) is an enveloped icosahedral DNA virus. HAdV infection can lead to immune system damage, resulting in decreased numbers and compromised function of T cells and B cells. It can also cause an imbalanced Th1/Th2 ratio and dysregulation of pro-inflammatory and anti-inflammatory cytokines. Objective: To investigate the serum levels of interleukin (IL)-13 and IL-17A in children with HAdV pneumonia. Methods: Pediatric patients diagnosed with HAdV pneumonia were divided into a non-severe group or a severe group based on the severity of their condition. Patients in the severe group were further classified into good and poor prognosis subgroups. We collected 2-2.5 mL of venous blood from each patient, which was then centrifuged. Using an ELISA detection kit, we determined the concentrations of IL-13 and IL-17A. Results: Patients with a severe condition exhibited significantly higher serum concentrations of IL-13 and IL-17A than the non-severe cases. Out of 50 severe cases, 32 had good prognoses, while 18 cases showed poor prognoses. Patients with poor prognoses showed significantly higher serum concentrations of IL-13 compared to those with good prognoses. Conclusion: Serum concentrations of IL-13 and IL-17A are potential diagnostic markers for pediatric patients with severe HAdV pneumonia. Additionally, they demonstrate good predictive value for a poor prognosis in severe pneumonia cases.

Is the mouse nose a miniature version of a rat nose? A computational comparative study.

BACKGROUND AND OBJECTIVE: Although the mouse is a widely used animal model in biomedical research, there are few published studies on its nasal aerodynamics, potentially due to its small size. It is not appropriate to assume that mice and rats' nasal structure and airflow characteristics are the same because the ratio of nasal surface area to nasal volume and body weight is much higher in a mouse than in a rat. The aim of this work is to use anatomically accurate image-based computational fluid dynamic modeling to quantitatively reveal the characteristics of mouse nasal airflow and mass transport that haven't been detailed before and find key differences to that of rat nose, which will deepen our understanding of the mouse's physiological functions. METHODS: We created an anatomically accurate 3D computational nasal model of a B6 mouse using postmortem high-resolution micro-CT scans and simulated the airflow distribution and odor transport patterns under restful breathing conditions. The deposition pattern of airborne particles was also simulated and validated against experimental data. In addition, we calculated the gas chromatograph efficiency of odor transport in the mouse employing the theoretical plate concept and compared it with previous studies involving cat and rat models. RESULTS: Similar to the published rat model, respiratory and olfactory flow regimes are clearly separated in the mouse nasal cavity. A high-speed dorsal medial (DM) stream was observed, which enhances the delivery speed and efficiency of odor to the ethmoid (olfactory) recess (ER). The DM stream split into axial and secondary paths in the ER. However, the secondary flow in the mouse is less extensive than in the rat. The gas chromatograph efficiency calculations suggest that the rat may possess a moderately higher odorant transport efficiency than that of the mouse due to its more complex ethmoid recess structure and extensive secondary flow. However, the mouse's nasal structure seems to adapt better to varying airflow velocity. CONCLUSIONS: Due to the inherent structural disparities, the rat and mouse models exhibit moderate differences in airflow and mass transport patterns, potentially impacting their olfaction and other behavioral habits.

Tissue/Organ Adaptable Bioelectronic Silk-Based Implants.

Implantable bioelectronic devices, designed for both monitoring and modulating living organisms, require functional and biological adaptability. Pure silk is innovatively employed, which is known for its excellent biocompatibility, to engineer water-triggered, geometrically reconfigurable membranes, on which functions can be integrated by Micro Electro Mechanical System (MEMS) techniques and specially functionalized silk. These devices can undergo programmed shape deformations within 10 min once triggered by water, and thus establishing stable bioelectronic interfaces with natively fitted geometries. As a testament to the applicability of this approach, a twining peripheral nerve electrode is designed, fabricated, and rigorously tested, demonstrating its efficacy in nerve modulation while ensuring biocompatibility for successful implantation.

Parasubthalamic calretinin neurons modulate wakefulness associated with exploration in male mice.

The parasubthalamic nucleus (PSTN) is considered to be involved in motivation, feeding and hunting, all of which are highly depending on wakefulness. However, the roles and underlying neural circuits of the PSTN in wakefulness remain unclear. Neurons expressing calretinin (CR) account for the majority of PSTN neurons. In this study in male mice, fiber photometry recordings showed that the activity of PSTNCR neurons increased at the transitions from non-rapid eye movement (non-REM, NREM) sleep to either wakefulness or REM sleep, as well as exploratory behavior. Chemogenetic and optogenetic experiments demonstrated that PSTNCR neurons were necessary for initiating and/or maintaining arousal associated with exploration. Photoactivation of projections of PSTNCR neurons revealed that they regulated exploration-related wakefulness by innervating the ventral tegmental area. Collectively, our findings indicate that PSTNCR circuitry is essential for the induction and maintenance of the awake state associated with exploration.

The Diagnostic Value of Procalcitonin in Patients with Severe Acute Pancreatitis: A Meta-Analysis.

The role of procalcitonin in diagnosing severe acute pancreatitis has not been clearly assessed. This meta-analysis aims to evaluate the overall diagnostic accuracy of procalcitonin as a biomarker for severe acute pancreatitis. Medline (via PubMed), Embase, Web of Science, Cochrane Library, China National Knowledge Infrastructure, and China WanFang Data were searched systematically for prospective studies reporting procalcitonin as a diagnostic marker of severe acute pancreatitis before August 31, 2021. Sensitivity, specificity, and other measures of the accuracy of procalcitonin in the diagnosis of severe acute pancreatitis were pooled by Stata 15.0 software. Heterogeneity was evaluated by I2 test, and the quality of included studies was evaluated by using the Quality Assessment of Diagnostic Accuracy Studies-2 system. Further, the sources of heterogeneity were verified using meta-regression and subgroup analysis, and the publication bias was evaluated by the Deeks' funnel plot. A total of 18 studies meeting the inclusion criteria were included, containing 1764 patients. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and area under the receiver operating characteristic curve of procalcitonin for diagnosing severe acute pancreatitis were as follows: 0.80 (95% CI: 0.73-0.86), 0.84 (95% CI: 0.78-0.88), 4.95 (95% CI: 3.46-7.09), 0.23 (95% CI: 0.16-0.34), 21.26 (95% CI: 11.09-40.74), 0.89 (95% CI: 0.86-0.92). Also, P > .05 suggested no significant publication bias. Current evidence indicates that procalcitonin has good sensitivity and diagnostic accuracy for severe acute pancreatitis. However, the findings should be carefully used as routine evidence in diagnosing patients with severe acute pancreatitis alone because of the limited number of included studies and high heterogeneity.

GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression.

Rapid eye movement (REM) sleep disturbances are prevalent in various psychiatric disorders. However, the neural circuits that regulate REM sleep remain poorly understood. Here, we found that in male mice, optogenetic activation of rostromedial tegmental nucleus (RMTg) GABAergic neurons immediately converted REM sleep to arousal and then initiated non-REM (NREM) sleep. Conversely, laser-mediated inactivation completely converted NREM to REM sleep and prolonged REM sleep duration. The activity of RMTg GABAergic neurons increased to a high discharge level at the termination of REM sleep. RMTg GABAergic neurons directly converted REM sleep to wakefulness and NREM sleep via inhibitory projections to the laterodorsal tegmentum (LDT) and lateral hypothalamus (LH), respectively. Furthermore, LDT glutamatergic neurons were responsible for the REM sleep-wake transitions following photostimulation of the RMTgGABA-LDT circuit. Thus, RMTg GABAergic neurons are essential for suppressing the induction and maintenance of REM sleep.

Poly(ethylene oxide)-Based Composite Electrolyte with Lithium-Doped High-Entropy Oxide Ceramic Enabled Robust Solid-State Lithium-Metal Batteries.

Solid polymer electrolytes using poly(ethylene oxide) (PEO) as matrix are mostly applied due to the superior Li+ transfer ability of oxyethyl chain. However, the high crystallinity, low oxidation potential window, and insufficient mechanical strength hinder PEO deployment in solid-state batteries. Here, a novel composite solid electrolyte combined PEO with a lithium-doped high-entropy oxide (Li0.25 HEO) ceramic powder is presented, which exhibits excellent properties for solid-state lithium metal battery applications. On one hand, the rich oxygen vacancies of Li0.25 HEO surface are favorable to capturing anionic groups (e. g. TFSI- ), reinforcing the Li+ dissociation. On the other hand, Li0.25 HEO with abundant Lewis acid sites markedly promotes the PEO oxidation potential window. Additionally, the incorporation of Li0.25 HEO ceramic powder can effectively inhibit the PEO crystallization and enhance the mechanic strength of the composite electrolyte as well. The assembled solid-state lithium metal battery based on the composite solid electrolyte exhibits high rate capacity and durable cycle performance, showing potential development and application prospects.

Airflow and nanoparticle deposition in rat nose under various breathing and sniffing conditions: a computational evaluation of the unsteady effect.

Accurate prediction of nanoparticle (1~100 nm) deposition in the rat nasal cavity is important for assessing the toxicological impact of inhaled nanoparticles as well as for potential therapeutic applications. A quasi-steady assumption has been widely adopted in the past investigations on this topic, yet the validity of such simplification under various breathing and sniffing conditions has not been carefully examined. In this study, both steady and unsteady computational fluid dynamics (CFD) simulations were conducted in a published rat nasal model under various physiologically realistic breathing and sniffing flow rates. The transient airflow structures, nanoparticle transport and deposition patterns in the whole nasal cavity and the olfactory region were investigated and compared with steady state simulation of equivalent flow rate. The results showed that (1) the quasi-steady flow assumption for cyclic flow was valid for over 70% of the cycle period during all simulated breathing and sniffing conditions in the rat nasal cavity, or the unsteady effect was only significant during the transition between the respiratory phases; (2) yet the quasi-steady assumption for nanoparticle transport was not valid, except in the vicinity of peak respiration. In general, the total deposition efficiency of nanoparticle during cyclic breathing would be lower than that of steady state due to the unsteady effect on particle transport and deposition, and further decreased with the increase of particle size, sniffing frequency, and flow rate. In the contrary, previous study indicated that for micro-scale particles (0.5~4µm), the unsteady effect would increase deposition efficiencies in rat nasal cavity. Combined, these results suggest that the quasi-steady assumption of nasal particle transport during cycling breathing should be used with caution for an accurate assessment of the toxicological and therapeutic impact of particle inhalation. Empirical equations and effective steady state approximation derived in this study are thus valuable to estimate such unsteady effects in future applications.

N-doped carbon coated anatase TiO2 nanoparticles as superior Na-ion battery anodes.

N-doped carbon coated TiO2 nanoparticles (TiO2@NC) were synthesized through a simple two-step route, in which dopamine was simultaneously utilized as both nitrogen and carbon sources. With TiO2@NC applied in the Na-ion battery (SIB) anodes, the continuous and uniform N-doped carbon layer can not only enhance the electrical conductivity of TiO2 and facilitate the surface pseudocapacitive process, but also serve as a buffer layer to accommodate the volume expansion during the sodiation-desodiation processes. The as-prepared TiO2@NC exhibits excellent electrochemical performance when utilized as the SIB anodes, which delivers a remarkably high reversible capacity of 250.2 mAh g-1 at a rate of 0.25C (84 mA g-1) after 200 cycles and still retains 122.1 mAh g-1 at 10C (3.35 A g-1) even after 3000 cycles accompanied with a 95.3% retention of the maximum capacity, outperforming most of the reported TiO2/C-based composites as SIB anodes. To our best knowledge, the preparation of TiO2@NC with dopamine as both nitrogen and carbon sources and its application in the SIB anodes are reported for the first time.

Computational modeling and validation of human nasal airflow under various breathing conditions.

The human nose serves vital physiological functions, including warming, filtration, humidification, and olfaction. These functions are based on transport phenomena that depend on nasal airflow patterns and turbulence. Accurate prediction of these airflow properties requires careful selection of computational fluid dynamics models and rigorous validation. The validation studies in the past have been limited by poor representations of the complex nasal geometry, lack of detailed airflow comparisons, and restricted ranges of flow rate. The objective of this study is to validate various numerical methods based on an anatomically accurate nasal model against published experimentally measured data under breathing flow rates from 180 to 1100ml/s. The numerical results of velocity profiles and turbulence intensities were obtained using the laminar model, four widely used Reynolds-averaged Navier-Stokes (RANS) turbulence models (i.e., k-ε, standard k-ω, Shear Stress Transport k-ω, and Reynolds Stress Model), large eddy simulation (LES) model, and direct numerical simulation (DNS). It was found that, despite certain irregularity in the flow field, the laminar model achieved good agreement with experimental results under restful breathing condition (180ml/s) and performed better than the RANS models. As the breathing flow rate increased, the RANS models achieved more accurate predictions but still performed worse than LES and DNS. As expected, LES and DNS can provide accurate predictions of the nasal airflow under all flow conditions but have an approximately 100-fold higher computational cost. Among all the RANS models tested, the standard k-ω model agrees most closely with the experimental values in terms of velocity profile and turbulence intensity.

Facile synthesis of MnO2-embedded flower-like hierarchical porous carbon microspheres as an enhanced electrocatalyst for sensitive detection of caffeic acid.

Tailored designs/fabrications of hierarchical porous advanced electrode materials are of great importance for developing high-performance electrochemical sensors. Herein, we demonstrate a simple and low-cost in situ chemical approach for the facile synthesis of MnO2-embedded hierarchical porous carbon microspheres (MnO2/CM). By the characterizations of scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction and energy dispersive spectroscopy, we evidenced that the synthesized product were flower-like carbon microspheres (CM) assembled by the bent flakes with thickness of about several nanometers and MnO2 nanorods were highly dispersed and successfully decorated on the CM layers, resulting in a rough surface and three-dimensional microstructure. The greatest benefit from the combined porous CM with MnO2 nanorods is that the MnO2/CM modified electrode has the synergetic catalysis effect on the electro-oxidation of caffeic acid, leading to the remarkable increase in the electron transfer rate and significant decrease in the over-potential for the caffeic acid oxidation in contrast to the bare electrode and CM modified electrode. This implies that the prepared MnO2/CM can be employed as an enhanced electrocatalyst for the sensitive detection of caffeic acid. Under the optimum conditions, the anodic peak current of caffeic acid is linear with its concentration in the range of 0.01-15.00 µmol L-1, and a detection limit of 2.7 nmol L-1 is achieved based on S/N = 3. The developed sensor shows good selectivity, sensitivity, reproducibility, and also excellent recovery in the detections of real samples, revealing the promising practicality of the sensor for the caffeic acid detection.

Ag/N-doped reduced graphene oxide incorporated with molecularly imprinted polymer: An advanced electrochemical sensing platform for salbutamol determination.

In this work, the metallic silver and non-metallic nitrogen co-doped reduced graphene oxide (Ag-N-RGO) was first synthesized by a simple and cost-effective strategy, and then a molecularly imprinted polymer (MIP) was formed in situ at the surface of the prepared composite via electropolymerization of o-phenylenediamine in the presence of salbutamol as the template molecule. The electrochemical characterizations demonstrate that the bifunctional graphene-based composite shows improved catalytic performance than that of pristine graphene doped with one-component or none. The MIP sensor based on Ag-N-RGO owns high porous surface structure, resulting in the increased current response and enhanced recognition capacity than that of non-imprinted sensor. The outstanding performance of the developed sensor derives from the combined advantages of Ag-N-RGO with effective catalytic property and MIP with excellent selectivity. Under the optimal conditions, the electrochemical response of the developed sensor is linearly proportional to the concentration of salbutamol in the range of 0.03-20.00µmolL-1 with a low detection limit of 7 nmol L-1. The designed sensor has exhibited the multiple advantages such as low cost, simple manufacture, convenient use, excellent selectivity and good reproducibility. Finally, the proposed method has been extended for the determinations of salbutamol in human urine and pork samples, and the satisfactory recoveries between 98.9-105.3% are achieved.

Designed synthesis of a novel BiVO4-Cu2O-TiO2 as an efficient visible-light-responding photocatalyst.

A novel visible-light-responding BiVO4-Cu2O-TiO2 ternary heterostructure composite was successfully fabricated via the preparation of BiVO4-TiO2 followed by coupling with Cu2O through facile wet chemistry methods based on the strategy of energy gap engineering. The as-fabricated composite was characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, high-resolution transmission electron microscopy, UV-vis diffuse reflectance spectroscopy and X-ray photoelectron spectroscopy. Benefited from the rational design and construction, BiVO4-Cu2O-TiO2 exhibits a significantly enhanced photocatalytic activity for the degradation of rhodamine B (RhB) under the visible-light irradiation as compared with Cu2O and Cu2O-TiO2. Specifically, under the irradiation with an ordinary 9 W energy-saving fluorescent lamp for 8h, the photocatalytic degradation ratio of RhB for 5 wt%BiVO4-40 wt%Cu2O-TiO2 reaches 97.8%. The enhanced photocatalytic activity of BiVO4-Cu2O-TiO2 can be ascribed to the matched band edge positions of BiVO4, Cu2O and TiO2, the heterojunction formations among them as well as the lower charge transfer resistance, favoring the separation of the photo-generated electron-hole pairs. A possible mechanism of the visible-light photocatalytic degradation of RhB is also proposed.

An Olfactory Cilia Pattern in the Mammalian Nose Ensures High Sensitivity to Odors.

In many sensory organs, specialized receptors are strategically arranged to enhance detection sensitivity and acuity. It is unclear whether the olfactory system utilizes a similar organizational scheme to facilitate odor detection. Curiously, olfactory sensory neurons (OSNs) in the mouse nose are differentially stimulated depending on the cell location. We therefore asked whether OSNs in different locations evolve unique structural and/or functional features to optimize odor detection and discrimination. Using immunohistochemistry, computational fluid dynamics modeling, and patch clamp recording, we discovered that OSNs situated in highly stimulated regions have much longer cilia and are more sensitive to odorants than those in weakly stimulated regions. Surprisingly, reduction in neuronal excitability or ablation of the olfactory G protein in OSNs does not alter the cilia length pattern, indicating that neither spontaneous nor odor-evoked activity is required for its establishment. Furthermore, the pattern is evident at birth, maintained into adulthood, and restored following pharmacologically induced degeneration of the olfactory epithelium, suggesting that it is intrinsically programmed. Intriguingly, type III adenylyl cyclase (ACIII), a key protein in olfactory signal transduction and ubiquitous marker for primary cilia, exhibits location-dependent gene expression levels, and genetic ablation of ACIII dramatically alters the cilia pattern. These findings reveal an intrinsically programmed configuration in the nose to ensure high sensitivity to odors.