RESUMEN
Complex hydrides have played important roles in energy storage area. Here a complex hydride made of Li2 NH and LiBH4 was synthesized, which has a structure tentatively indexed using an orthorhombic cell with a space group of Pna21 and lattice parameters of a=10.121, b=6.997, and c=11.457â Å. The Li2 NH-LiBH4 sample (in a molar ratio of 1:1) shows excellent hydrogenation kinetics, starting to absorb H2 at 310â K, which is more than 100â K lower than that of pristine Li2 NH. Furthermore, the Li+ ion conductivity of the Li2 NH-LiBH4 sample is about 1.0×10-5 â S cm-1 at room temperature, and is higher than that of either Li2 NH or LiBH4 at 373â K. Those unique properties of the Li2 NH-LiBH4 complex render it a promising candidate for hydrogen storage and Li ion conduction.
RESUMEN
The calcium amidoborane hydrazinates, Ca(NH2BH3)2·nN2H4, were firstly synthesized by reacting different molar ratios of Ca(NH2BH3)2 and N2H4. In particular, Ca(NH2BH3)2 and N2H4 with a molar ratio of 1 : 2 crystallizes into the orthorhombic symmetry P212121 space group with the lattice parameters of a = 6.6239(4) Å, b = 13.7932(6) Å, c = 4.7909(2) Å. The dehydrogenations of calcium amidoborane hydrazinates are two-step reactions, exhibiting superior dehydrogenation properties compared with those of pristine Ca(NH2BH3)2. For Ca(NH2BH3)2-1/2N2H4, approximately 4.6 equiv. hydrogen (or 7.9 wt% hydrogen) can be released at 150 °C. Kinetic analysis shows that the activation energies for the two steps of hydrogen desorption from Ca(NH2BH3)2·2N2H4 are much lower than those of pristine Ca(NH2BH3)2, suggesting an improvement in the dehydrogenation kinetics of Ca(NH2BH3)2 after coordinating with N2H4. Isotopic labeling results show that the driving force for the dehydrogenation of calcium amidoborane hydrazinates is the combination mechanism of protonic hydrogen and hydridic hydrogen (H(δ+) and H(δ-)). In addition, initial H2 release from calcium amidoborane hydrazinates originates from the interaction of [-BH3] and N2H4, rather than [-BH3] and [-NH2] (in [-NH2BH3]).
RESUMEN
BACKGROUND: To investigate the effect of creatine and coenzyme Q10 (CoQ10) combination therapy on mild cognitive impairment (MCI) in Parkinson's disease (PD; PD-MCI) and its influences on plasma phospholipid (PL) levels in PD-MCI. METHODS: The demographic data of 75 PD-MCI patients who enrolled in this collaborative PD study were collected. These patients were evaluated using the Unified Parkinson's Disease Rating Scale (UPDRS) III and the Montreal Cognitive Assessment (MoCA). These 75 PD-MCI patients were randomly treated with creatine monohydrate 5 g b.i.d. and CoQ10 100 mg t.i.d. orally or placebo. MoCA evaluation and PL level measurements were performed after 12 and 18 months of treatment. RESULTS: After 12 and 18 months of treatment, the differences in the MoCA scores of the combination therapy and control groups were statistically significant (p < 0.05 at 12 months and p < 0.01 at 18 months), and the plasma PL levels of the combination therapy group were significantly lower than those of the control group (p < 0.01 at 12 months and p < 0.001 at 18 months). CONCLUSIONS: Combination therapy with creatine and CoQ10 could delay the decline of cognitive function in PD-MCI patients and could lower their plasma PL levels; therefore, this combination therapy may have a neuroprotective function.
Asunto(s)
Cognición/efectos de los fármacos , Disfunción Cognitiva/tratamiento farmacológico , Creatina/administración & dosificación , Enfermedad de Parkinson/complicaciones , Ubiquinona/análogos & derivados , Anciano , Disfunción Cognitiva/etiología , Quimioterapia Combinada , Femenino , Humanos , Masculino , Persona de Mediana Edad , Enfermedad de Parkinson/tratamiento farmacológico , Enfermedad de Parkinson/psicología , Ubiquinona/administración & dosificaciónRESUMEN
The lithiation of ethylenediamine by LiH is a stepwise process to form the partially lithiated intermediates LiN(H)CH2 CH2 NH2 and [LiN(H)CH2 CH2 NH2 ][LiN(H)CH2 CH2 N(H)Li]2 prior to the formation of dilithiated ethylenediamine LiN(H)CH2 CH2 N(H)Li. A reversible phase transformation between the partial and dilithiated species was observed. One dimensional {Lin Nn } ladders and three-dimensional network structures were found in the crystal structures of LiN(H)CH2 CH2 NH2 and LiN(H)CH2 CH2 N(H)Li, respectively. LiN(H)CH2 CH2 N(H)Li undergoes dehydrogenation with an activation energy of 181±8â kJ mol(-1) , whereas the partially lithiated ethylenediamine compounds were polymerized and released ammonia at elevated temperatures. The dynamical dehydrogenation mechanism of the dilithiated ethylenediamine compounds was investigated by using the Johnson-Mehl-Avrami equation.
RESUMEN
A facile method for synthesizing crystalline lithiated amines by ball milling primary amines with LiH was developed. The lithiated amines exhibit an unprecedented endothermic dehydrogenation feature in the temperature range of 150-250 °C, which shows potential as a new type of hydrogen storage material. Structural analysis and mechanistic studies on lithiated ethylenediamine (Li2EDA) indicates that Li may mediate the dehydrogenation through an α,ß-LiH elimination mechanism, creating a more energy favorable pathway for the selective H2 release.
RESUMEN
Neuroprotective strategies, including free radical scavengers, ion channel modulators, and anti-inflammatory agents, have been extensively explored in the last 2 decades for the treatment of neurological diseases. Unfortunately, none of the neuroprotectants has been proved effective in clinical trails. In the current study, we demonstrated that methylene blue (MB) functions as an alternative electron carrier, which accepts electrons from NADH and transfers them to cytochrome c and bypasses complex I/III blockage. A de novo synthesized MB derivative, with the redox center disabled by N-acetylation, had no effect on mitochondrial complex activities. MB increases cellular oxygen consumption rates and reduces anaerobic glycolysis in cultured neuronal cells. MB is protective against various insults in vitro at low nanomolar concentrations. Our data indicate that MB has a unique mechanism and is fundamentally different from traditional antioxidants. We examined the effects of MB in two animal models of neurological diseases. MB dramatically attenuates behavioral, neurochemical, and neuropathological impairment in a Parkinson disease model. Rotenone caused severe dopamine depletion in the striatum, which was almost completely rescued by MB. MB rescued the effects of rotenone on mitochondrial complex I-III inhibition and free radical overproduction. Rotenone induced a severe loss of nigral dopaminergic neurons, which was dramatically attenuated by MB. In addition, MB significantly reduced cerebral ischemia reperfusion damage in a transient focal cerebral ischemia model. The present study indicates that rerouting mitochondrial electron transfer by MB or similar molecules provides a novel strategy for neuroprotection against both chronic and acute neurological diseases involving mitochondrial dysfunction.
Asunto(s)
Proteínas del Complejo de Cadena de Transporte de Electrón/metabolismo , Azul de Metileno/farmacología , Mitocondrias/enzimología , Fármacos Neuroprotectores/farmacología , Enfermedad de Parkinson Secundaria/tratamiento farmacológico , Animales , Línea Celular , Proteínas del Complejo de Cadena de Transporte de Electrón/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Glucólisis/efectos de los fármacos , Masculino , Neuronas/enzimología , Neuronas/patología , Consumo de Oxígeno/efectos de los fármacos , Enfermedad de Parkinson Secundaria/inducido químicamente , Enfermedad de Parkinson Secundaria/enzimología , Enfermedad de Parkinson Secundaria/patología , Enfermedad de Parkinson Secundaria/fisiopatología , Ratas , Ratas Sprague-Dawley , Rotenona/efectos adversos , Rotenona/farmacología , Sustancia Negra/enzimología , Sustancia Negra/patología , Sustancia Negra/fisiopatología , Desacopladores/efectos adversos , Desacopladores/farmacologíaRESUMEN
Cs-Loaded MgO, a typical type of solid superbase, was used as a support to disperse and anchor Ru nanoparticles. Much enhanced Ru dispersion together with electron donation of Cs-loaded MgO enables Ru nanoparticles on Cs-loaded MgO to exhibit excellent activity and intrinsic activity for ammonia synthesis, superior to the conventional Ru/MgO catalyst and the state-of-the-art Cs modified Ru/MgO catalyst under identical reaction conditions.
RESUMEN
We have reported that the major endogenous estrogen, 17beta-estradiol (E2), protects against oxidative injury during ethanol withdrawal (EW) in a cultured hippocampal cell line (HT22). Here, we investigated whether the pro-oxidant nature of EW mediates opening of the mitochondrial membrane permeability transition pore (PTP) in a manner protected by E2. Excess PTP opening provokes mitochondrial membrane swelling (MMS) and the collapse of membrane potential (DeltaPsim). HT22 cells were collected at the end of ethanol exposure (100 mM) for 24 h or at 4 h of EW to assess MMS by monitoring absorbance decline at 540 nm and to assess DeltaPsim using flow cytometry. Protective effects of E2 on PTP were compared with an antioxidant butylated hydroxytoluene (BHT) and an E2 analog, ZYC26 [(3-hydroxy-2-adamantyl(1)-4-methyl-estra-1,3,5(10)-17-one], with higher antioxidant potency than E2. To assess cellular consequences of PTP opening, effects of a PTP inhibitor (cyclosporin A) on EW-induced cell death were assessed using the calcein assay. Major findings were that: 1) EW resulted in rapid MMS and DeltaPsim collapse; 2) cyclosporin A attenuated EW-induced cell death; and 3) E2 treatment restricted to the EW phase protected against the PTP opening more prominently than BHT and to a similar degree to ZYC26. These findings suggest that EW provokes PTP opening partly but not entirely through the pro-oxidant nature and that E2 counteracts EW-associated factors to protect against the PTP opening.
Asunto(s)
Estrógenos/farmacología , Etanol/farmacología , Proteínas de Transporte de Membrana Mitocondrial/fisiología , Membranas Mitocondriales/fisiología , Neuronas/fisiología , Adamantano/análogos & derivados , Adamantano/farmacología , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Estrona/análogos & derivados , Estrona/farmacología , Femenino , Hipocampo/citología , Hipocampo/fisiología , Humanos , Ratones , Proteínas de Transporte de Membrana Mitocondrial/efectos de los fármacos , Membranas Mitocondriales/efectos de los fármacos , Poro de Transición de la Permeabilidad Mitocondrial , Neuronas/efectos de los fármacos , Ovariectomía , PermeabilidadRESUMEN
NLRP6 inflammasome, one of the important intracellular innate immune sensors, has been shown to regulate immune responses. However, its roles in the intracerebral hemorrhage (ICH) are completely not clear. In the present study, we investigated the expression profile and biological roles of NLRP6 inflammasome in perihematomal brain tissues of mice subjected to ICH. In this study, we investigated the expression profile of NLRP6 inflammasome in the perihematomal brain tissues and explored the biological role of NLRP6 inflammasome upon acute brain injury in mice subjected to ICH. Increased expression of NLRP6 inflammasome was found in perihematomal brain tissues ranging from 6 h to 3 days, with a peak level at 1 day after ICH. Immunohistochemistry staining also showed that NLRP6 inflammasome was significantly increased in the perihematomal brain tissues at 1 day after ICH. Moreover, immunofluorescence staining showed that NLRP6 inflammasome was mainly colocalized in glial fibrillary acidic protein (GFAP)-positive astrocytes, while with little colocalized expression in NeuN-positive neurons and without expression in CD11b-positive microglia and CD31-positive endothelial cell in the perihematomal brain tissue of mice after ICH. Furthermore, NLRP6-/- ICH mice exhibited significantly higher brain water contents (BMCs), proinflammatory cytokines, NF-κB activity and neurological deficit scores when compared with the wild type (WT) ICH mice. In addition, we found that Toll-like receptor 4 (TLR4)-/- mice, as well as the TAK242 treated mice, had markedly lower expression of NLRP6 inflammasome expression in the perihematomal brain tissue at 1 day after ICH. Our data suggest that the upregulated NLRP6 inflammasome in perihematomal brain tissues attenuates ICH-induced brain injury.
RESUMEN
BACKGROUND: Important advances have been made in research into the mechanism of alcoholic liver disease (ALD) over the past few years, but the role of liver sinusoidal endothelial cell (LSEC) in ALD has not been elucidated adequately. This study was undertaken to investigate the effect of ethanol on fenestrae of LSECs in rats. METHODS: A rat model of alcoholic liver disease was established by means of direct intragastric instillation of ethanol. Fifty-five rats of experimental (35 rats) and control (20) groups were sacrificed at the end of 4, 8, 12 weeks respectively, and also at the end of 12-week abstinence. After heart perfusion, the liver tissue was fixed and stained with hematoxylin and eosin for observation of serial changes of LSEC-fenestrae under a transmission electron microscope. RESULTS: Normal LESC was flat with a nucleus and organelles arranged regularly. The distal cytoplasm displayed as a lamina with many fenestrae, lacking the basement membrane(BM) underneath the endothelium. At the end of 4-week alcohol feeding, the number of fenestrae decreased at the distal cytoplasm in some LSECs, without the formation of the BM underneath the endothelium. At the end of 8 weeks, the number of fenestrae decreased significantly or even disappeared. The BM began to develop incompletely underneath the endothelium, while the active fibroblast appeared. At the end of 12 weeks, the number of fenestrae decreased more significantly and the complete BM could even be seen. But the changes were mostly limited in the single or adjoining sinus, and fibrosis was scarcely formed. At the end of 12-week abstinence, defenestration and formation of the endothelial BM lightened significantly. CONCLUSIONS: Defenestration and formation of the BM in LSECs develop gradually with the chronic stimulation of ethanol. Hepatic sinusoidal capillarization and fibrosis will be seen if their state is more serious. These early changes, i.e., limited and regional defenestration and capillarization may be the basis of alcoholic peri-fibrosis. This kind of hepatic fibrosis is reversible after removal of etiological factors.
Asunto(s)
Células Endoteliales/patología , Hepatopatías Alcohólicas/patología , Hígado/irrigación sanguínea , Animales , Membrana Basal/patología , Masculino , Microscopía Electrónica , Ratas , Ratas WistarRESUMEN
BACKGROUND: Cerebral microbleeds (CMBs) have been observed in the elderly and have been regarded as a manifestation of small vessel disease (SVD). Cerebral and glomerular SVD may have a common source of pathogenesis because these organs are closely connected through anatomic and hemodynamic similarities. The purpose of this study was to clarify the associations between kidney biomarker levels and CMBs in hypertensive patients. METHODS: The presence and number of CMBs were assessed on susceptibility-weighted imaging. We calculated the urinary albumin/creatinine ratio (UACR) from morning spot urine and the estimated glomerular filtration rate (eGFR) in serum samples. Serum cystatin C (CysC) was measured with an automated particle-enhanced turbidimetric immunoassay. RESULTS: UACR and CysC levels were higher in the patients with CMBs than those without, and the eGFR was lower in the patients with CMBs than those without. A logistic regression analysis indicates that eGFR and UACR were independently associated with the prevalence of deep or infratentorial CMBs. The odds ratio (OR) (95% confidence interval (CI)) of eGFR and UACR was 1.95 (1.37-3.27) and 2.25 (1.66-4.46), respectively. CysC was independently associated with CMBs in both deep or infratentorial and lobar locations. The ORs (95% CI) were 2.59 (1.57-6.22) and 1.57 (1.15-4.85), respectively. Furthermore, CysC exhibited fair diagnostic value for CMBs, with an area under the curve of 0.80. CONCLUSIONS: Kidney biomarker levels are associated with the presence of CMB in hypertensive patients without a history of transient ischemic attack (TIA) or stroke, independent of conventional risk factors, and CysC was a better marker for CMBs than eGFR and UACR.
Asunto(s)
Albuminuria , Creatinina/sangre , Cistatina C/sangre , Tasa de Filtración Glomerular , Hipertensión/complicaciones , Hemorragia Intracraneal Hipertensiva , Anciano , Albuminuria/diagnóstico , Albuminuria/metabolismo , Biomarcadores/sangre , Biomarcadores/orina , Femenino , Humanos , Hemorragia Intracraneal Hipertensiva/diagnóstico , Hemorragia Intracraneal Hipertensiva/etiología , Hemorragia Intracraneal Hipertensiva/metabolismo , Pruebas de Función Renal/métodos , Imagen por Resonancia Magnética/métodos , Masculino , Persona de Mediana Edad , Factores de Riesgo , Estadística como AsuntoRESUMEN
OBJECTIVE: To study the influence of alcohol on the liver sinusoids endothelial cell (LSEC) fenestrae of rats. METHODS: Setting up the rat model of alcoholic liver disease by orogastric administration of alcohol, then kill the experimental and control groups of rats at the end of 4 weeks, 8 weeks and 12 weeks after alcohol feeding, and also at the end of another 12 weeks after balance foods feeding succeeding with alcohol feeding for 12 weeks. Staining the liver tissue by means of HE method and observing the successive change of LSEC fenestrae by transmission electron microscope. RESULTS: The normal LSEC was flat with nucleus and organelle arranged regularly. The distal cytoplasm displayed as lamina with many fenestrae, not accompanied by basement membrane (BM) formation under the endothelial cell. At the end of 4 weeks of alcohol feeding, fenestrae decreased at the partial distal LSEC cytoplasm, but no BM developed. At the end of 8 weeks, fenestrae decreased significantly, even disappeared, with the BM developed incompletely under the endothelial cell. Concomitantly, fibroblast with active function developed. At the end of 12 weeks, the changes became more obvious; the complete BM could even be seen. However, this kind of changes was mostly limited in the single or adjoining sinusoids, as well as with little widespread formation of fibrosis. At the end of 12 weeks of stopping alcohol feeding, defenestrae and development of BM attenuated obviously. CONCLUSION: The defenestrae and BM of LSEC develop gradually with the chronic alcohol stimulation. Sinusoid capillarization and liver fibrosis even form when significant changes happen. The early change of the limited defenestrae and capillarization may be the basis of alcohol periportal fibrosis formation. This kind of liver fibrosis can be reversible after stopping alcohol feeding.
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Endotelio/efectos de los fármacos , Etanol , Cirrosis Hepática Experimental/patología , Hepatopatías Alcohólicas/patología , Animales , Membrana Basal/patología , Endotelio/patología , Hígado/irrigación sanguínea , Hígado/patología , Masculino , Ratas , Ratas WistarRESUMEN
Recent studies have shown that kidney dysfunction is associated with cerebral microbleeds (CMB). Cystatin C is a more useful measurement than creatinine-based estimating equations for evaluating kidney function. The purpose of this study was to clarify the relationship between cystatin C levels and CMB in patients with acute cerebral stroke. This cross-sectional study included a total of 485 patients with acute ischemic stroke and 129 patients with cerebral hemorrhage. The serum levels of cystatin C were significantly higher in acute cerebral stroke patients with CMB than in those without (p<0.001). Multivariate logistic regression analyses showed that for each single standard deviation increase of cystatin C levels, there was a significant increase in the presence of CMB after adjusting for age and sex, and after additional adjustment for cardiovascular risk factors, silent lacunar infarction, and white matter hyperintensity in patients with acute stroke. The odds ratio (95% confidence interval) in patients with acute cerebral infarction and cerebral hemorrhage were 2.92 (1.81-6.93) and 2.98 (1.76-6.97), respectively. The present study suggests that elevated levels of cystatin C are associated with the presence of CMB in acute stroke patients, independent of conventional risk factors.
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Hemorragia Cerebral/sangre , Cistatina C/sangre , Accidente Cerebrovascular/sangre , Anciano , Proteína C-Reactiva/análisis , Estudios Transversales , Femenino , Tasa de Filtración Glomerular , Humanos , Modelos Logísticos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Oportunidad Relativa , Factores de RiesgoRESUMEN
p38 is a mitogen-activated protein kinase and mediates cell growth, cell differentiation, and synaptic plasticity. The aim of this study is to determine the extent to which p38 plays a role in maintaining mitochondrial respiration in male and female mice under a normal condition. To achieve this aim, we have generated transgenic mice that lack p38 in cerebellar Purkinje neurons by crossing Pcp2 (Purkinje cell protein 2)-Cre mice with p38(loxP/loxP) mice. Mitochondria from cerebellum were then isolated from the transgenic and wild-type mice to measure mitochondrial respiration using XF24 respirometer. The mRNA and protein expression of cytochrome c oxidase (COX) in cerebellum were also measured using RT-PCR and immunoblot methods. Separately, HT22 cells were used to determine the involvement of 17ß-estradiol (E2) and COX in mitochondrial respiration. The genetic knockout of p38 in Purkinje neurons suppressed the mitochondrial respiration only in male mice and increased COX expression only in female mice. The inhibition of COX by sodium azide (SA) sharply suppressed mitochondrial respiration of HT22 cells in a manner that was protected by E2. These data suggest that p38 is required for the mitochondrial respiration of male mice. When p38 is below a normal level, females may maintain mitochondrial respiration through COX up-regulation.
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Cerebelo/metabolismo , Estrógenos/farmacología , Mitocondrias/fisiología , Oxígeno/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , Animales , Respiración de la Célula/efectos de los fármacos , Respiración de la Célula/fisiología , Células Cultivadas , Cerebelo/efectos de los fármacos , Femenino , Masculino , Ratones Noqueados , Ratones Transgénicos , Prostaglandina-Endoperóxido Sintasas/metabolismo , Factores Sexuales , Distribución Tisular , Proteínas Quinasas p38 Activadas por Mitógenos/genéticaRESUMEN
We investigated whether abrupt ethanol withdrawal (EW) age-specifically inhibits a key mitochondrial enzyme, cytochrome c oxidase (COX), and whether estrogen mitigates this problem. We also tested whether this possible effect of EW involves a substrate (cytochrome c) deficiency that is associated with proapoptotic Bcl2-associated X protein (BAX) and mitochondrial membrane swelling. Ovariectomized young, middle age, and older rats, with or without 17ß-estradiol (E2) implantation, underwent repeated EW. Cerebelli were collected to measure COX activity and the mitochondrial membrane swelling using spectrophotometry and the mitochondrial levels of cytochrome c and BAX using an immunoblot method. The loss of COX activity and the mitochondrial membrane swelling occurred only in older rats under control diet conditions but occurred earlier, starting in the young rats under EW conditions. E2 treatment mitigated these EW effects. EW increased mitochondrial BAX particularly in middle age rats but did not alter cytochrome c. Collectively EW hastens but E2 delays the age-associated loss of COX activity. This EW effect is independent of cytochrome c but may involve the mitochondrial overload of BAX and membrane vulnerability.
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Envejecimiento/efectos de los fármacos , Envejecimiento/metabolismo , Depresores del Sistema Nervioso Central/efectos adversos , Complejo IV de Transporte de Electrones/fisiología , Etanol/efectos adversos , Síndrome de Abstinencia a Sustancias/metabolismo , Animales , Complejo IV de Transporte de Electrones/química , Femenino , Estabilidad Proteica , Ratas , Ratas Endogámicas F344 , Síndrome de Abstinencia a Sustancias/etiología , Especificidad por Sustrato/genética , Regulación hacia Arriba/genética , Proteína X Asociada a bcl-2/metabolismoRESUMEN
Intermittent hypoxia (IH) conditioning minimizes neurocognitive impairment and stabilizes brain mitochondrial integrity during ethanol withdrawal (EW) in rats, but the mitoprotective mechanism is unclear. We investigated whether IH conditioning protects a key mitochondrial enzyme, cytochrome c oxidase (COX), from EW stress by inhibiting mitochondrially directed apoptotic pathways involving cytochrome c, Bax, or phosphor-P38 (pP38). Male rats completed two cycles of a 4-wk ethanol diet (6.5%) and 3 wk of EW. An IH program consisting of 5-10 bouts of 5-8 min of mild hypoxia (9.5-10% inspired O(2)) and 4 min of reoxygenation for 20 consecutive days began 3 days before the first EW period. For some animals, vitamin E replaced IH conditioning to test the contributions of antioxidant mechanisms to IH's mitoprotection. During the second EW, cerebellar-related motor function was evaluated by measuring latency of fall from a rotating rod (Rotarod test). After the second EW, COX activity in cerebellar mitochondria was measured by spectrophotometry, and COX, cytochrome c, Bax, and pP38 content were analyzed by immunoblot. Mitochondrial protein oxidation was detected by measuring carbonyl contents and by immunochemistry. Earlier IH conditioning prevented motor impairment, COX inactivation, depletion of COX subunit 4, protein carbonylation, and P38 phosphorylation during EW. IH did not prevent cytochrome c depletion during EW, and Bax content was unaffected by EW ± IH. Vitamin E treatment recapitulated IH protection of COX, and P38 inhibition attenuated protein oxidation during EW. Thus IH protects COX and improves cerebellar function during EW by limiting P38-dependent oxidative damage.
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Consumo de Bebidas Alcohólicas/efectos adversos , Cerebelo/enzimología , Complejo IV de Transporte de Electrones/metabolismo , Etanol/efectos adversos , Hipoxia/enzimología , Mitocondrias/enzimología , Estrés Oxidativo , Síndrome de Abstinencia a Sustancias/prevención & control , Animales , Antioxidantes/farmacología , Apoptosis , Conducta Animal , Western Blotting , Cerebelo/efectos de los fármacos , Cerebelo/patología , Cerebelo/fisiopatología , Citocromos c/metabolismo , Modelos Animales de Enfermedad , Hipoxia/patología , Hipoxia/fisiopatología , Hipoxia/psicología , Imidazoles/farmacología , Masculino , Mitocondrias/efectos de los fármacos , Mitocondrias/patología , Actividad Motora , Estrés Oxidativo/efectos de los fármacos , Fosforilación , Carbonilación Proteica , Inhibidores de Proteínas Quinasas/farmacología , Piridinas/farmacología , Ratas , Ratas Sprague-Dawley , Tiempo de Reacción , Espectrofotometría , Síndrome de Abstinencia a Sustancias/enzimología , Síndrome de Abstinencia a Sustancias/etiología , Síndrome de Abstinencia a Sustancias/patología , Síndrome de Abstinencia a Sustancias/fisiopatología , Síndrome de Abstinencia a Sustancias/psicología , Factores de Tiempo , Vitamina E/farmacología , Proteína X Asociada a bcl-2/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
We investigated whether protein kinase p38 plays a role in the brain-aging changes associated with repeated ethanol withdrawal (EW). Ovariectomized young, middle-age and older rats, with or without 17ß-estradiol (E2) implantation, received a 90-day ethanol with repeated withdrawal. They were tested for active pP38 expression in cerebellar Purkinje neurons and whole-cerebellar lysates using immunohistochemistry and enzyme-linked immunosorbent assay, respectively. They were also tested for the Rotarod task to determine the behavioral manifestation of cerebellar neuronal stress and for reactive oxygen species (ROS) and mitochondrial protein carbonyls to determine oxidative mechanisms. Middle-age EW rats showed higher levels of pP38-positive Purkinje neurons/cerebellar lysates, which coincided with increased mitochondrial protein oxidation than other diet/age groups. Exacerbated motor deficit due to age-EW combination also began at the middle-age. In comparison, ROS contents peaked in older EW rats. E2 treatment mitigated each of the EW effects to a different extent. Collectively, pP38 may mediate the brain-aging changes associated with pro-oxidant EW at vulnerable ages and in vulnerable neurons in a manner protected by estrogen.