Evaluation of fatigue in young female adults due to repeated exposure to heat in summer and cold in winter.

PURPOSE: The magnitude of fatigue (MF) from psychological and physiological responses during repeated exposure to heat in summer and during repeated exposure to cold in winter was evaluated to test two hypotheses on fatigue models. The first hypothesis is that exposure time (ET, min) would be a factor determining the MF and the second hypothesis is that the same fatigue models as a function of the number of exposure repetitions (NR) could be applied to both repeated exposure to heat in summer and cold in winter. METHODS: In summer, eight young adult female subjects with clothing insulation (Icl, clo) of 0.3 clo first stayed in the control room at 26 ℃ for 15 min, moved to the main testing room at 30 ℃ for 25 min, 33 °C for 15 min, or 36 ℃ for 10 min, and then returned to the control room. The product of air temperature difference (ΔTa, ℃) and ET was designed to be almost equal among these latter three conditions. The exposure was repeated five times. In winter, the same female subjects with Icl of 0.84 clo first stayed in the control room at 24 ℃ for 15 min, moved to the main testing room at 18 ℃ for 30 min, 15 ℃ for 20 min, or 12 ℃ for 15 min, and then returned to the control room. Again, the product of ΔTa and ET was designed to be equal among these latter three conditions. The exposure was repeated four times. The scores of subjective fatigue feeling (SFF) and salivary amylase value (SAV) were recorded when the subjects returned to the control room. Tympanic temperature, skin temperatures and local sweat rates (Sw, mg/cm2/min) at chest, forearm, front thigh, and front shin, and ECG were continuously monitored, except for Sw in the winter experiment. RESULTS: In the summer experiment, the SFF showed a threshold value at ΔTa = 4 ℃ but continuously increased with NR at ΔTa = 7 ℃ and 10 ℃. It was not correlated with ECG variables, but was positively correlated with SAV (R2 = 0.50) and the mean Sw (R2 = 0.76) at ΔTa = 7 ℃ and 10 ℃. In the winter experiment, the SFF showed a threshold value at ΔTa = - 6 ℃ but continuously increased with NR at ΔTa = - 9 ℃ and - 12 ℃. It was correlated with SAV at ΔTa = - 9 ℃ (R2 = 0.77) and score of LF: HF ratio at ΔTa = - 6 ℃ and - 9 ℃ (R2 = 0.49). CONCLUSION: It was confirmed that ET may be related to the MF and that different fatigue models may be applied dependent on ΔTa during repeated exposure to heat in summer and during repeated exposure to cold in winter. Thus, the two hypotheses were verified.

Underlying mechanism of diurnal change in thermal sensation response at high relative humidity.

Diurnal changes in physiological and behavioral responses to constant relative humidity (RH) were investigated in summer to validate a hypothesis that thermal sensation responses (TSR) vary with the diurnal cycle while maintained at stable RH's of 60, 70, or 80%. Seven lightly clothed male subjects were exposed to one of three RH while air temperature (Ta) was held at 28 °C from 9:00-18:30. Mean skin temperature (T‾sk) and tympanic temperature (Tty) were monitored at 2-min intervals throughout the experimental period. Cutaneous warm and cool sensation thresholds and stratum corneum water content (SCWC) on the anterior forearm, posterior forearm, and anterior thigh, finger blood flow rate (SkBF) were measured by a thermal stimulator controlled by a Peltier element (Intercross-230; Intercross, Co., Tokyo, Japan), a water content of stratum corneum monitor (Corneometer CM825 & MPA 5; Integral Co., Tokyo, Japan), and a laser flowmeter (ALF21; Advance Co., Tokyo, Japan), accordingly at the end of three periods: 9:30-10:30, 13:30-14:30, and 17:30-18:30. The TSR and the thermal comfort response were also recorded using subjective scales of thermal sensation and comfort thresholds at the end of three periods. The Tty and mean skin temperature (T‾sk) remained unchanged during the day under all RH conditions. Temperature difference between warm and cold sensation thresholds and SkBF decreased slightly towards the evening under all RH conditions whereas SCWC increased from the morning to the evening at 60% and 80% RH. Behaviorally, the subjects responded more than "slightly warm" at 70%, and 80% RH, and then the TSR changed significantly (p < 0.05) to less than "slightly warm" in the evening, although T̅sk remained unchanged at 34.0 °C. The results from the previous study in winter was reconfirmed, and the data verify the hypothesis that TSR changes diurnally even though subjects are exposed to a constant and high RH's and stable Ta. It was confirmed in summertime as previously shown in wintertime that the combined reduction in SkBF to increases SCWC when exposed to high RH explains the altered TSR although Ta are unchanged.

Diurnal change in psychological and physiological responses to consistent relative humidity.

Diurnal changes in physiological and psychological responses to consistent relative humidity (RH) conditions were investigated in the present study. Lightly clothed six male and six female subjects participated in the first experiment at 40% and 50% RH, and seven male and seven female subjects participated in the second experiment at 60%, 70%, and 80% RH. Both experiments were conducted at 28 °C air temperature (Ta) from 9:00-18:30. Skin temperatures, local heat flux rates and tympanic temperature (Tty) were monitored at 2-min intervals throughout the experimental period. Body weight loss and oxygen consumption rate were measured during the 9:30-10:30, 13:30-14:30, and 17:30-18:30 periods. Thermal sensation and thermal comfort responses were recorded at the same periods. The amount of heat loss was greater than metabolic heat production (M) in the male subjects but was well balanced with M in the female subjects. A morning increase in Tty at 50%-80% RH was observed, and mean skin temperature (T‾sk) at 70% and 80% RH was significantly higher (p < 0.05) than Tsk at 40% and 50% RH in both subject groups. Although difference in the relationship between thermal sensation and T‾sk based on sex was confirmed, diurnal changes in thermal sensation were observed in both subject groups based on the responses of "warm" in the morning but "neutral" or "slightly warm" in the evening at 70% and 80% RH. This result demonstrates that high RH may be acceptable in the late afternoon and evening at 28 °C and indicates that dynamic control of RH during the daytime (e.g., low RH in the morning and high RH in late afternoon) may be beneficial to save energy when using air-conditioning.

Effect of change in ambient temperature on core temperature of female subjects during the daytime and its sex differences.

Tympanic temperature (Tty), skin temperature, and regional dry heat loss were measured continuously in eight female subjects under three conditions: (1) stepwise increases in ambient temperature (Ta) from 26 °C at 09:00 to 30 °C at 18:00, (2) steady Ta at 28 °C from 09:00 to 18:00, and (3) stepwise decreases in Ta from 30 °C at 09:00 to 26 °C at 18:00. Oxygen consumption, body weight loss, thermal sensation, and comfort levels were periodically recorded. The Tty increased significantly (p < 0.01) from 36.1 ± 0.36 °C to 36.6 ± 0.23 °C at 18:00 under condition 1 but remained virtually unchanged under conditions 2 and 3. Thermal comfort was observed at 15:00 and 17:00 under condition 3, whereas subjects reported that they felt slightly cool at 15:00. The rate of body heat storage (S), changes in Tty, mean skin temperature ([Formula: see text]sk), and mean body temperature during each period were calculated, and confirmed that changes in [Formula: see text]sk was correlated with S. Diurnal changes in core temperature (Tc) appeared to be more dependent on diurnal rhythm than on changes in Ta, except when Ta increased continuously. Thus, it may be difficult to predict diurnal changes in women's Tc using a body-heat-balance equation during thermal transient.

Physiological responses to changes in relative humidity under thermally neutral, warm and hot conditions.

Four hypothetical thermophysiological responses to changes in relative humidity (Rh) under thermally neutral, warm, and hot conditions were proposed for a person at rest. Under thermally neutral and warm conditions, the first hypothetical response to an increase in Rh was a decrease in mean skin temperature (T¯sk) due to increase in mean evaporation rate (E¯sk), and the second hypothetical response to a decrease in Rh was a decrease, an increase, or no change in T¯sk, depending on changes in the E¯sk. Under hot conditions, the third hypothetical response to an increase in the Rh was an increase in T¯sk or decrease in T¯sk upon decrease in the Rh due to changes in E¯sk, and the forth hypothetical response to an increase in Rh was an increase in T¯sk due to increase in the peripheral blood flow rate (SkBF). To test these hypotheses, the T¯sk and E¯sk of four young male volunteers were measured at 28°C, 30°C, or 32°C while the Rh was maintained at 40% or 80% Rh for 60min after 20min exposure at 60% Rh (control condition). In a second experiment, the T¯sk, E¯sk, and SkBF of five young male volunteers were measured at 34°C-40% Rh or 36°C-40% Rh, or 34°C-70% Rh or 36°C-70% Rh for 60min after 20min exposure at 28°C-60% Rh (control condition). The first hypothesis was partly supported by the findings that the T¯sk was lower than the control values at 28°C-80% Rh and the E¯sk was higher than the control values at 80% Rh at any tested temperature. The second hypothesis was partly supported by the findings that the T¯sk was lower than the control values at 28°C-40% Rh, and there were small changes in both T¯sk and E¯sk at 30°C-40% Rh. The third and fourth hypotheses were supported by the findings that the T¯sk at 36°C-70% Rh was significantly higher (p<0.01) than at 36°C-40% Rh, the E¯sk was significantly higher (p<0.01) at 70% Rh than at 40% Rh, and SkBF was positively correlated with T¯sk.

Effect of change in ambient temperature on core temperature during the daytime.

In this study, the hypothesis is tested that continuous increases in ambient temperature (Ta) during daytime would give elevated core and skin temperatures, and consequently better thermal sensation and comfort. Rectal temperature (Tre), skin temperatures and regional dry heat losses at 7 sites were continuously measured for 10 Japanese male subjects in three thermal conditions: cond. 1, stepwise increases in Ta from 26 °C at 9 h00 to 30 °C at 18 h00; cond. 2, steady Ta at 28 °C from 9 h00 to 18 h00 and cond. 3, stepwise decreases in Ta from 30 °C at 9 h00 to 26 °C at 18 h00. Oxygen consumption was measured and thermal sensation and comfort votes were monitored at 15 min intervals. Body weight loss was measured at 1 h intervals. While Tre increased continuously in the morning period in any condition, it increased to a significantly greater (p<0.05) 36.9±0.3 °C at 18 h00 in cond. 1 relative to 36.7±0.28 °C in Cond. 2 and 36.5±0.37 °C in cond. 3. Better thermal comfort was observed in the afternoon and the evening in Cond.1 as compared with the other 2 conditions. Thus, a progressive and appropriate increase in Ta may induce optimal cycle in core temperature during daytime, particularly for a resting person.

Comfortable indoor lighting conditions for LEDlights evaluated from psychological and physiological responses.

Comfortable light emitting diode (LED) lighting conditions were examined for psychological and physiological responses. Eight male and eight female subjects participated in a series of experiments. They were exposed to light emitted from LED lights of 3000 K in the ranges of 150-400 lux and 1500-5000 lux; 4000 K in the ranges of 140-640 lux and 2000-5000 lux; and 5000 K in the ranges of 70-270 lux and 2500-7000 lux. Illuminance at a given correlated color temperature was first maintained at the lowest value in the designated range and then increased in a stepwise manner. On reaching the highest value in the range, it was then decreased to the lowest value in a stepwise manner. The subjects were required to indicate their perception of brightness, glare, and comfort at the controlled illuminance levels. Electrocardiogram and electroencephalogram data were monitored continuously throughout exposure. The results showed that the boundary illuminances estimated from psychological and physiological responses did not significantly differ between male and female subjects, but differed markedly from those of fluorescent lights tested in a previous study. The high boundaries of the LED lights were higher than those of the fluorescent lights. The low boundaries of the LED lights at 3000 K and 4000 K were higher than those of the fluorescent lights, but the boundaries at 5000 K were lower than those of the fluorescent lights. These differences may be due to differences in the spectral distribution and luminance for a given illuminance between LED and fluorescent lights.

Do green-blocking glasses enhance the nonvisual effects of white polychromatic light?

BACKGROUND: It is well known that light containing the blue component stimulates the intrinsically photosensitive retinal ganglion cells (ipRGCs) and plays a role in melatonin suppression and pupillary constriction. In our previous studies, we verified that simultaneous exposure to blue and green light resulted in less pupillary constriction than blue light exposure. Hence, we hypothesized that the nonvisual effects of polychromatic white light might be increased by blocking the green component. Therefore, we conducted an experiment using optical filters that blocked blue or green component and examined the nonvisual effects of these lights on pupillary constriction and electroencephalogram power spectra. METHODS: Ten healthy young males participated in this study. The participant sat on a chair with his eyes facing an integrating sphere. After 10 min of light adaptation, the participant's left eye was exposed to white pulsed light (1000 lx; pulse width 2.5 ms) every 10 s with a blue-blocking glasses, a green-blocking glasses, or control glasses (no lens), and pupillary constriction was measured. Then, after rest for 10 min, the participant was exposed a continuous white light of 1000 lx with a blue- or green-blocking glasses or control glasses and electroencephalogram was measured. RESULTS: Pupillary constriction with the blue-blocking glasses was significantly less than that observed with the green-blocking glasses. Furthermore, pupillary constriction under the green-blocking glasses was significantly greater than that observed with the control glasses. CONCLUSIONS: A reduction in the green component of light facilitated pupillary constriction. Thus, the effects of polychromatic white light containing blue and green components on ipRGCs are apparently increased by removing the green component.

Diurnal variation in the core interthreshold zone and its relation to cutaneous sensation threshold zone.

BACKGROUND: The core interthreshold zone (CIZ) is defined as the range between temperatures at the onset of shivering and sweating. Its circadian or diurnal variation has not been extensively studied. The present study examined whether the CIZ is subject to a diurnal rhythm. In addition, according to the previous finding that the CIZ was proportionally correlated with peripheral interthreshold zone (PIZ), it was also examined whether cutaneous sensation threshold zone (CSZ), a determinant of the PIZ, is correlated with the CIZ. METHODS: The CIZ and the CSZ were measured in ten Japanese men who underwent three experiments in a single day on the morning, afternoon, and evening in the 2014 experiment (so-called single-day experiment) and six Japanese men underwent the same experiments on the morning of day 1, the afternoon of day 2, and the evening of day 3 in the 2015 experiment (so-called multiple-day experiment). Air temperature was controlled at 20-24 °C. Each subject wore a suit perfused with 25 °C water at a rate of 600 cm3/min and exercised on an ergometer at 50% of their maximum work rate for 10-15 min until their rate of sweating increased. They then remained seated without exercising until their oxygen uptake increased. Rectal temperature, skin temperatures at seven sites, the sweating rate at the forehead, and oxygen uptake were continuously monitored throughout experiment. Cutaneous warm and cold sensation thresholds at three sites were measured using 1- and 2-cm2 probes. RESULTS: The results from the single-day experiment demonstrated a small change in the CIZ and core temperature prior to exercise (T c-init) whereas those from the multiple-day experiment demonstrated continuous increase in the CIZ and T c-init. The CSZ measured with a 1-cm2 probe was inversely proportional to the average skin temperature at three sites prior to measurement (T sk-av). CONCLUSION: The results suggested that the CIZ may be not dependent on time of a day but Tc-init per se and may not be associated with the CSZ.

The core interthreshold zone during exposure to red and blue light.

BACKGROUND: This study tested the hypothesis that the core interthreshold zone (CIZ) changes during exposure to red or blue light via the non-visual pathway, because it is known that light intensity affects the central nervous system. We conducted a series of human experiments with 5 or 10 male subjects in each experiment. METHODS: The air temperature in the climatic chamber was maintained at 20 to 24°C. The subjects wore suits perfused with 25°C water at a rate of 600 cm3/min. They exercised on an ergometer at 50% of their maximum work rate for 10 to 15 minutes until sweating commenced, and then remained continuously seated without exercise until their oxygen uptake increased. The rectal temperature and skin temperatures at four sites were monitored using thermistors. The sweating rate was measured at the forehead with a sweat rate monitor. Oxygen uptake was monitored with a gas analyzer. The subjects were exposed to red or blue light at 500 lx and 1000 lx in both summer and winter. RESULTS: The mean CIZs at 500 lx were 0.23 ± 0.16°C under red light and 0.20 ± 0.10°C under blue light in the summer, and 0.19 ± 0.20°C under red light and 0.26 ± 0.24°C under blue light in the winter. The CIZs at 1000 lx were 0.18 ± 0.14°C under red light and 0.15 ± 0.20°C under blue light in the summer, and 0.52 ± 0.18°C under red light and 0.71 ± 0.28°C under blue light in the winter. A significant difference (P <0.05) was observed in the CIZs between red and blue light at 1000 lx in the winter, and significant seasonal differences under red light (P <0.05) and blue light (P <0.01) were also observed at 1000 lx. CONCLUSIONS: The present study demonstrated that dynamic changes in the physiological effects of colors of light on autonomic functions via the non-visual pathway may be associated with the temperature regulation system.

The effect of season and light intensity on the core interthreshold zone.

The hypothesis tested in the present study is a seasonal difference in the core interthreshold zone (CIZ), as we suggested in an earlier study that individual awareness of heat may change the CIZ due to thermoregulatory behavior. A series of human experiments were carried out in a climatic chamber in January and August of 2009 and January of 2010. The air temperature in the chamber was controlled at 20-24°C. Subjects wore a water-perfused suit that was perfused with 25°C water at a rate of 600 cc/min. They exercised on an ergometer at 50% of their maximum work rate for 10-15 min until their sweating rate increased and then remained seated without exercise until oxygen uptake increased. Subjects' rectal temperature and skin temperatures at four sites were monitored by thermistors. The sweating rate was measured at the forehead with a sweat rate monitor (SKD 4000, Skinos Co.). Oxygen uptake was monitored with a gas analyzer (Respiromonitor RM-300i, Minato Med. Science Co.). In the 2009 winter experiment, 5 male subjects were exposed to lighting of 36 cd/m(2)/1,050 lx, and in the 2009 summer and 2010 winter experiments, 10 male subjects were exposed to lighting of 18 cd/m2/510 lx. The results showed that the CIZ of 0.69±0.29°C (n=22, data from 2005-2007 experiments) at 36 cd/m(2) and that of 0.37±0.17°C (n=10) at 18 cd/m(2) in summer were greater than the CIZ of 0.37±0.13°C (n=5) at 36 cd/m(2) and that of 0.18±0.17°C (n=10) at 18 cd/m(2) in winter, and thus demonstrated a seasonal difference in the CIZ as well as an effect of lighting conditions on the CIZ.

Individual variability in the core interthreshold zone as related to body physique, somatotype, and physical constitution.

For evaluating the effect of body physique, somatotype, and physical constitution on individual variability in the core interthreshold zone (CIZ), data from 22 healthy young Japanese male subjects were examined. The experiment was carried out in a climatic chamber in which air temperature was maintained at 20-24 degrees C. The subjects' body physique and the maximum work load were measured. Somatotype was predicted from the Heath-Carter Somatotype method. In addition, factors reflecting physical constitution, for example, susceptibility to heat and cold, and quality of sleep were obtained by questionnaire. The subjects wore a water-perfused suit which was perfused with water at a temperature of 25 degrees C and at a rate of 600 cc/min, and exercised on an ergometer at 50% of their maximum work rate for 10-15 min until their sweating rate increased. They then remained continuously seated without exercise until shivering increased. Rectal temperature (T(re)) and skin temperatures at four sites were monitored by thermistors, and sweating rate was measured at the forehead with a sweat rate monitor. Oxygen uptake was monitored with a gas analyzer. The results showed individual variability in the CIZ. According to the reciprocal cross-inhibition (RCI) theory, thermoafferent information from peripheral and core sensors is activated by T(re), mean skin temperature (T(sk)), and their changes. Since T(sk) was relatively unchanged, the data were selected to eliminate the influence of the core cooling rate on the sensor-to-effector pathway before RCI, and the relationship between the CIZ and the various factors was then analyzed. The results revealed that susceptibility to heat showed a good correlation with the CIZ, indicating that individual awareness of heat may change the CIZ due to thermoregulatory behavior.

Individual variability in the peripheral and core interthreshold zones.

The purpose of the study was to investigate the degree of subject variability in the peripheral and core temperature thresholds of the onset of shivering and sweating. Nine healthy young male subjects participated in three trials. In the first two trials, wearing only shorts, they were exposed to air temperatures of 5 degrees C and 40 degrees C until the onset of shivering and sweating, respectively. In the second experiment, subjects wore a water perfused suit that was perfused with 25 degrees C water at a rate of 600 cc/min. They exercised on an ergometer at 50% of their maximum work rate for 10-15 min. At the onset of sweating, the exercise was terminated, and they remained seated until the onset of shivering, as reflected in oxygen uptake. In the first two trials, rectal temperature (Tre) was stable, despite displacements in skin temperature (Tsk), whereas in the third trial, Tsk (measured at four sites) was almost constant (30-32 degrees C), and the thermoregulatory responses were initiated due to changes in Tre alone. The results of the first two trials established the peripheral interthreshold zone, whereas the results of the third trial established the core interthreshold zone. The results demonstrated individual variability in the peripheral and core interthreshold zones, a proportional correlation between both zones (r=0.87), and a relatively higher contribution of adiposity in both zones as compared with those of other non-thermal factors such as height, weight, body surface area, surface area-to mass ratio, and the maximum work load.