Laser Remote Sensing of Lake Kinneret by Compact Fluorescence LiDAR.

Harmful algal blooms in freshwater reservoirs became a steady phenomenon in recent decades, so instruments for monitoring water quality in real time are of high importance. Modern satellite remote sensing is a powerful technique for mapping large areas but cannot provide depth-resolved data on algal concentrations. As an alternative to satellite techniques, laser remote sensing is a perspective technique for depth-resolved studies of fresh or seawater. Recent progress in lasers and electronics makes it possible to construct compact and lightweight LiDARs (Light Detection and Ranging) that can be installed on small boats or drones. LiDAR sensing is an established technique; however, it is more common in studies of seas rather than freshwater reservoirs. In this study, we present an experimental verification of a compact LiDAR as an instrument for the shipborne depth profiling of chlorophyll concentration across the freshwater Lake Kinneret (Israel). Chlorophyll depth profiles of 3 m with a 1.5 m resolution were measured in situ, under sunlight conditions. A good correlation (R2 = 0.89) has been established between LiDAR signals and commercial algae profiler data. A non-monotonic algae depth distribution was observed along the boat route during daytime (Tiberias city-Jordan River mouth-Tiberias city). The impact of high algal concentration on water temperature laser remote sensing has been studied in detail to estimate the LiDAR capability of in situ simultaneous measurements of temperature and chlorophyll concentration.

Experimental Testing of an Approach to Establishing Combined Toxicity of Ternary Nanoparticle Mixtures.

Our studies of exposure to binary mixtures of nanoparticles (TiO2 + SiO2; TiO2 + Al2O3 and SiO2 + Al2O3) based on mathematical modelling show that their combined subchronic toxicity can either be of an additive type or deviate from it depending on the outcome, dose ratio, and levels of effect. To characterize the type of toxicity of ternary mixtures of nanoparticles, we successfully tested a previously developed approach for assessing the combined toxicity of metal ions. In this approach, the effects are classified by a null, positive, or negative change in the toxicity of binary nanoparticle mixtures when modeled against the toxicity of the third agent added.

Changes in the Cardiotoxic Effects of Lead Intoxication in Rats Induced by Muscular Exercise.

Exposure to lead is associated with an increased risk of cardiovascular diseases. Outbred white male rats were injected with lead acetate intraperitoneally three times a week and/or were forced to run at a speed of 25 m/min for 10 min 5 days a week. We performed noninvasive recording of arterial pressure, electrocardiogram and breathing parameters, and assessed some biochemical characteristics. Electrophoresis in polyacrylamide gel was used to determine the ratio of myosin heavy chains. An in vitro motility assay was employed to measure the sliding velocity of regulated thin filaments on myosin. Isolated multicellular preparations of the right ventricle myocardium were used to study contractility in isometric and physiological modes of contraction. Exercise under lead intoxication normalized the level of calcium and activity of the angiotensin-converting enzyme in the blood serum, normalized the isoelectric line voltage and T-wave amplitude on the electrocardiogram, increased the level of creatine kinase-MB and reduced the inspiratory rate. Additionally, the maximum sliding velocity and the myosin heavy chain ratio were partly normalized. The effect of exercise under lead intoxication on myocardial contractility was found to be variable. In toto, muscular loading was found to attenuate the effects of lead intoxication, as judged by the indicators of the cardiovascular system.

Observations of acoustic noise bursts accompanying nonlinear internal gravity waves on the continental shelf off New Jersey.

Anomalously large, transient fluctuations of acoustical noise intensity, up to 4-5 orders of magnitude above the background, were observed with single-hydrophone receiver units (SHRUs) and on the L-shaped horizontal and vertical line array of hydrophones (HVLA) in the Shallow Water 2006 experiment on the continental shelf off New Jersey. Here, temporal and spatial properties of these noise bursts are investigated. As tidally generated nonlinear internal waves (NIWs) move across the site of the experiment from the shelf break toward the coast, they form trains of localized, soliton-like waves with up to 25-35 m displacement of isopycnal surfaces. The NIW trains consecutively cross the positions of five SHRUs and HVLA that are located about 5-8 km from each other along a line perpendicular to the coast. The noise bursts were observed when a NIW train passed through locations of the corresponding acoustic receivers. Turbulence of the water flow, saltation, and bedload of marine sediments were the dominant causes of the acoustic noise bursts caused by NIWs at different frequency bands. On near-bottom hydrophones, the most energetic part of the observed noise bursts is attributed to collisions of suspended sediment particles with each other, the sensor, and the seafloor.

Looking for the LOAEL or NOAEL Concentration of Nickel-Oxide Nanoparticles in a Long-Term Inhalation Exposure of Rats.

Rats were exposed to nickel oxide nano-aerosol at a concentration of 2.4 ± 0.4 µg/m3 in a "nose only" inhalation setup for 4 h at a time, 5 times a week, during an overall period of 2 weeks to 6 months. Based on the majority of the effects assessed, this kind of exposure may be considered as close to LOAEL (lowest observed adverse effect level), or even to NOAEL (no observed adverse effect level). At the same time, the experiment revealed genotoxic and allergic effects as early as in the first weeks of exposure, suggesting that these effects may have no threshold at all.

Cardioinotropic Effects in Subchronic Intoxication of Rats with Lead and/or Cadmium Oxide Nanoparticles.

Subchronic intoxication was induced in outbred male rats by repeated intraperitoneal injections with lead oxide (PbO) and/or cadmium oxide (CdO) nanoparticles (NPs) 3 times a week during 6 weeks for the purpose of examining its effects on the contractile characteristics of isolated right ventricle trabeculae and papillary muscles in isometric and afterload contractions. Isolated and combined intoxication with these NPs was observed to reduce the mechanical work produced by both types of myocardial preparation. Using the in vitro motility assay, we showed that the sliding velocity of regulated thin filaments drops under both isolated and combined intoxication with CdO-NP and PbO-NP. These results correlate with a shift in the expression of myosin heavy chain (MHC) isoforms towards slowly cycling ß-MHC. The type of CdO-NP + PbO-NP combined cardiotoxicity depends on the effect of the toxic impact, the extent of this effect, the ratio of toxicant doses, and the degree of stretching of cardiomyocytes and muscle type studied. Some indices of combined Pb-NP and CdO-NP cardiotoxicity and general toxicity (genotoxicity included) became fully or partly normalized if intoxication developed against background administration of a bioprotective complex.

Using discrete low-frequency components of shipping noise for gassy sediment characterization in shallow water.

This work shows that normal-mode attenuation coefficients can be extracted from ship noise and used to estimate the sound speed in gas-saturated sediments. In an experiment in the Sea of Galilee, a research vessel served as a noise source approaching a vertical hydrophone array at a constant speed. Twelve narrow-band components of the vessel noise in the frequency band 20-100 Hz were identified and mode filtered to estimate the normal-mode attenuation coefficients. The inversion results indicate that the sound speed in the sediments was approximately 170 m/s.

Passive geoacoustic inversion in the Mid-Atlantic Bight in the presence of strong water column variability.

Empirical Green's functions are obtained for 31 paths in a highly dynamic coastal ocean by cross-correlation of ambient and shipping noise recorded in the Shallow Water 2006 experiment on a horizontal line array and a single hydrophone about 3600 m from the array. Using time warping, group speeds of three low-order normal modes are passively measured in the 10-110 Hz frequency band and inverted for geoacoustic parameters of the seabed. It is demonstrated that, despite very strong sound speed variations caused by nonlinear internal waves, noise interferometry can be successfully used to acoustically characterize the seafloor on a continental shelf.

Manifestation of Systemic Toxicity in Rats after a Short-Time Inhalation of Lead Oxide Nanoparticles.

Outbred female rats were exposed to inhalation of lead oxide nanoparticle aerosol produced right then and there at a concentration of 1.30 ± 0.10 mg/m3 during 5 days for 4 h a day in a nose-only setup. A control group of rats were sham-exposed in parallel under similar conditions. Even this short-time exposure of a relatively low level was associated with nanoparticles retention demonstrable by transmission electron microscopy in the lungs and the olfactory brain. Some impairments were found in the organism's status in the exposed group, some of which might be considered lead-specific toxicological outcomes (in particular, increase in reticulocytes proportion, in δ-aminolevulinic acid (δ-ALA) urine excretion, and the arterial hypertension's development).

Whispering gallery waves localized near circular isobaths in shallow water.

Sound propagation in an area of shallow sea with circular isobaths and the bottom depth increasing towards their curvature center is considered. Such an area could be part of a bay, a lagoon, or a lake with bowl-like bottom relief. The possible formation of whispering gallery waves localized in the vicinity of curved isobaths is considered. Simple criteria for the formation of such whispering gallery due to horizontal refraction are derived; their transverse modal structure is described analytically in terms of Bessel functions and using the Wentzel-Kramers-Brillouin-Jeffreys approximation. The corresponding azimuthal wavenumbers and radial mode functions are computed. Dispersion of such waves is also investigated, and the possibility of their experimental observation is discussed.

Normal mode dispersion and time warping in the coastal ocean.

Simple, analytically solvable models of normal mode propagation in the coastal ocean are developed and applied to study the effect of the seafloor bathymetry on modal travel times. Within the adiabatic approximation, horizontal inhomogeneity of the waveguide is found to change the modal dispersion curves in a way that helps separation of the modal components of the acoustic field using the time-warping transform. It is shown that moderate seafloor slopes can lead to surprisingly large errors in retrieved geoacoustic parameters and cause a positive bias in bottom sound speed estimates if horizontal refraction is ignored.

Toxic Effects of Low-Level Long-Term Inhalation Exposures of Rats to Nickel Oxide Nanoparticles.

Rats were exposed to nickel oxide nanoparticles (NiO-NP) inhalation at 0.23 ± 0.01 mg/m³ for 4 h a day 5 times a week for up to 10 months. The rat organism responded to this impact with changes in cytological and some biochemical characteristics of the bronchoalveolar lavage fluid along with a paradoxically little pronounced pulmonary pathology associated with a rather low chronic retention of nanoparticles in the lungs. There were various manifestations of systemic toxicity, including damage to the liver and kidneys; a likely allergic syndrome as indicated by some cytological signs; transient stimulation of erythropoiesis; and penetration of nickel into the brain from the nasal mucous membrane along the olfactory pathway. Against a picture of mild to moderate chronic toxicity of nickel, its in vivo genotoxic effect assessed by the degree of DNA fragmentation in nucleated blood cells (the RAPD test) was pronounced, tending to increasing with the length of the exposure period. When rats were given orally, in parallel with the toxic exposure, a set of innocuous substances with differing mechanisms of expected bioprotective action, the genotoxic effect of NiO-NPs was found to be substantially attenuated.

Variability of phase and amplitude fronts due to horizontal refraction in shallow water.

The variability of the interference pattern of a narrow-band sound signal in a shallow water waveguide in the horizontal plane in the presence of horizontal stratification, in particular due to linear internal waves, is studied. It is shown that lines of constant phase (a phase front) and lines of constant amplitude/envelope (an amplitude front) for each waveguide mode may have different directions in the spatial vicinity of the point of reception. The angle between them depends on the waveguide's parameters, the mode number, and the sound frequency. Theoretical estimates and data processing methodology for obtaining these angles from experimental data recorded by a horizontal line array are proposed. The behavior of the angles, which are obtained for two episodes from the Shallow Water 2006 (SW06) experiment, show agreement with the theory presented.

Combined Subchronic Toxicity of Aluminum (III), Titanium (IV) and Silicon (IV) Oxide Nanoparticles and Its Alleviation with a Complex of Bioprotectors.

Stable suspensions of metal/metalloid oxide nanoparticles (MeO-NPs) obtained by laser ablation of 99.99% pure elemental aluminum, titanium or silicon under a layer of deionized water were used separately, or in three binary combinations, or in a ternary combination to induce subchronic intoxications in rats. To this end, the MeO-NPs were repeatedly injected intraperitoneally (i.p.) 18 times during 6 weeks before measuring a large number of functional, biochemical, morphological and cytological indices for the organism's status. In many respects, the Al2O3-NP was found to be the most toxic species alone and the most dangerous component of the combinations studied. Mathematical modeling with the help of the Response Surface Methodology showed that, as well as in the case of any other binary toxic combinations previously investigated by us, the organism's response to a simultaneous exposure to any two of the MeO-NP species under study was characterized by a complex interaction between all possible types of combined toxicity (additivity, subadditivity or superadditivity of unidirectional action and different variants of opposite effects) depending on which outcome this type was estimated for and on effect and dose levels. With any third MeO-NP species acting in the background, the type of combined toxicity displayed by the other two remained virtually the same or changed significantly, becoming either more or less unfavorable. Various harmful effects produced by the (Al2O3-NP + TiO2-NP + SiO2-NP)-combination, including its genotoxicity, were substantially attenuated by giving the rats per os during the entire exposure period a complex of innocuous bioactive substances expected to increase the organism's antitoxic resistance.

Interference pattern of the sound field in the presence of an internal Kelvin wave in a stratified lake.

Internal Kelvin waves (IKWs) initiated by rotation of the Earth are one of the main hydrodynamic phenomena in large stratified lakes where baroclinic Rossby radius of deformation is smaller than the horizontal scale of the lake. IKWs can be identified using the spectra of internal waves, where in the presence of IKWs, the inertial frequency is at maximum. IKWs play a rather important role in the lake's dynamics for different processes, both in the water layer and sediment, especially at the periphery of lake. Due to influence of internal waves on the sound propagation, acoustical methods can be used for estimation of behaviour of IKWs. In this paper, the spatiotemporal variability of the mid-frequency (∼1 kHz) sound field in the presence of IKWs in a deep stratified Lake Kinneret is studied using numerical simulations based on normal-mode theory. Due to the specific character of perturbation of the water layer, IKWs can cause specific variations of interference pattern, in particular, a significant shift of the sound interference pattern both in spatial and frequency domain. These shifts can be easily measured and used for reconstruction of IKW parameters.

Bottom attenuation estimation using sound intensity fluctuations due to mode coupling by nonlinear internal waves in shallow water.

Analyses of fluctuations of low frequency signals (300 ± 30 Hz) propagating in shallow water in the presence of nonlinear internal waves (NIWs) in the Shallow Water 2006 experiment are carried out. Signals were received by a vertical line array at a distance of ∼20 km from the source. A NIW train was moving totally inside of the acoustic track, and the angle between the wave front of the NIW and the acoustic track in the horizontal plane was ∼10°. It is shown that the spectrum of the sound intensity fluctuations contains peaks corresponding to the coupling of pairs of propagating modes. Analysis of spectra at different hydrophone depths, and also summed over depth allows the authors to estimate attenuation in the bottom sediments.

Attenuation of Combined Nickel(II) Oxide and Manganese(II, III) Oxide Nanoparticles' Adverse Effects with a Complex of Bioprotectors.

Stable suspensions of NiO and Mn3O4 nanoparticles (NPs) with a mean (±s.d.) diameter of 16.7±8.2 and 18.4±5.4 nm, respectively, purposefully prepared by laser ablation of 99.99% pure nickel or manganese in de-ionized water, were repeatedly injected intraperitoneally (IP) to rats at a dose of 2.5 mg/kg 3 times a week up to 18 injections, either alone or in combination. A group of rats was injected with this combination with the background oral administration of a "bio-protective complex" (BPC) comprising pectin, vitamins A, C, E, glutamate, glycine, N-acetylcysteine, selenium, iodide and omega-3 PUFA, this composition having been chosen based on mechanistic considerations and previous experience. After the termination of injections, many functional and biochemical indices and histopathological features (with morphometric assessment) of the liver, spleen, kidneys and brain were evaluated for signs of toxicity. The Ni and Mn content of these organs was measured with the help of the atomic emission and electron paramagnetic resonance spectroscopies. We obtained blood leukocytes for performing the RAPD (Random Amplified Polymorphic DNA) test. Although both metallic NPs proved adversely bio-active in many respects considered in this study, Mn3O4-NPs were somewhat more noxious than NiO-NPs as concerns most of the non-specific toxicity manifestations and they induced more marked damage to neurons in the striatum and the hippocampus, which may be considered an experimental correlate of the manganese-induced Parkinsonism. The comparative solubility of the Mn3O4-NPs and NiO-NPs in a biological medium is discussed as one of the factors underlying the difference in their toxicokinetics and toxicities. The BPC has attenuated both the organ-systemic toxicity and the genotoxicity of Mn3O4-NPs in combination with NiO-NPs.

Toxicodynamic and toxicokinetic descriptors of combined chromium (VI) and nickel toxicity.

After repeated intraperitoneal injections of nickel and chromium (VI) salts to rats, we found, and confirmed by mathematical modeling, that their combined subchronic toxicity can either be of additive type or depart from it (predominantly toward subadditivity) depending on the effect assessed. Against the background of moderate systemic toxicity, the combination under study proved to possess a marked additive genotoxicity assessed by means of the random amplification of polymorphic DNA test. We also demonstrated that chromium and nickel reciprocally influenced the retention of these metals in some organs (especially in the spleen) but not their urinary excretion in this study.

Some characteristics of free cell population in the airways of rats after intratracheal instillation of copper-containing nano-scale particles.

We used stable water suspensions of copper oxide particles with mean diameter 20 nm and of particles containing copper oxide and element copper with mean diameter 340 nm to assess the pulmonary phagocytosis response of rats to a single intratracheal instillation of these suspensions using optical, transmission electron, and semi-contact atomic force microscopy and biochemical indices measured in the bronchoalveolar lavage fluid. Although both nano and submicron ultrafine particles were adversely bioactive, the former were found to be more toxic for lungs as compared with the latter while evoking more pronounced defense recruitment of alveolar macrophages and especially of neutrophil leukocytes and more active phagocytosis. Based on our results and literature data, we consider both copper solubilization and direct contact with cellular organelles (mainly, mitochondria) of persistent particles internalized by phagocytes as probable mechanisms of their cytotoxicity.

Subchronic toxicity of copper oxide nanoparticles and its attenuation with the help of a combination of bioprotectors.

In the copper metallurgy workplace air is polluted with condensation aerosols, which a significant fraction of is presented by copper oxide particles<100 nm. In the scientific literature, there is a lack of their in vivo toxicity characterization and virtually no attempts of enhancing organism's resistance to their impact. A stable suspension of copper oxide particles with mean (±SD) diameter 20±10 nm was prepared by laser ablation of pure copper in water. It was being injected intraperitoneally to rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week up to 19 injections. In parallel, another group of rats was so injected with the same suspension against the background of oral administration of a "bio-protective complex" (BPC) comprising pectin, a multivitamin-multimineral preparation, some amino acids and fish oil rich in ω-3 PUFA. After the termination of injections, many functional and biochemical indices for the organism's status, as well as pathological changes of liver, spleen, kidneys, and brain microscopic structure were evaluated for signs of toxicity. In the same organs we have measured accumulation of copper while their cells were used for performing the Random Amplification of Polymorphic DNA (RAPD) test for DNA fragmentation. The same features were assessed in control rats infected intraperitoneally with water with or without administration of the BPC. The copper oxide nanoparticles proved adversely bio-active in all respects considered in this study, their active in vivo solubilization in biological fluids playing presumably an important role in both toxicokinetics and toxicodynamics. The BPC proposed and tested by us attenuated systemic and target organs toxicity, as well as genotoxicity of this substance. Judging by experimental data obtained in this investigation, occupational exposures to nano-scale copper oxide particles can present a significant health risk while the further search for its management with the help of innocuous bioprotectors seems to be justified.