Toward the Required Detection Limits for Volatile Organic Constituents in Marine Environments with Infrared Evanescent Field Chemical Sensors.

A portable sensor system for the simultaneous detection of multiple environmentally relevant volatile organic compounds (VOCs) in real seawater based on Fourier transform infrared fiber-optic evanescent wave spectroscopy (FT-IR-FEWS) was developed. A cylindrical silver halide (AgX) fiber with an ethylene/propylene copolymer (E/P-co) coated flattened segment was used as an active optical transducer. The polymer membrane enriches the hydrophobic analytes, while water is effectively excluded from the penetration depth of the evanescent field. Determination of multicomponent mixtures (i.e., 10 VOCs in real-world seawater samples) collected in Arcachon Bay, France revealed a high accuracy and reproducibility with detection limits down to 560 ppb. The measurement showed no significant influence from changing water conditions (e.g., salinity, turbidity, and temperature or other interfering substances). The time constants for 90% saturation of the polymer ranged from 20 to 60 min. The sensor system is capable of being transported for on-site monitoring of environmental pollutants in aqueous matrices with efficient long-term stability, thus showing great potential to be utilized as an early warning system.

Fourier transform infrared spectroscopy on external perturbations inducing secondary structure changes of hemoglobin.

The secondary structure of proteins and their conformation are intimately related to their biological functions. In this study, heat-induced changes in the secondary structure and conformation of hemoglobin were investigated via infrared attenuated total reflection (IR-ATR) spectroscopy. The secondary structure changes of hemoglobin were derived from IR-ATR spectra using second derivatives and curve fitting. Thereby, the thermal denaturation temperature ranges and the secondary structure changes with temperature were revealed. More detailed information on the secondary structure and conformation was elucidated via two-dimensional infrared correlation spectroscopy. This study deciphers the detailed conformational behavior of hemoglobin molecular changes along with temperature, and creates a general methodological framework for analyzing the heat-induced behavior of biomacromolecules.

Coherent beam combining in atmospheric channels using gated backscatter.

This paper introduces the concept of atmospheric channels and describes a possible approach for the coherent beam combining of lasers of an optical phased array (OPA) in a turbulent atmosphere. By using the recently introduced sparse spectrum harmonic augmentation method, a comprehensive simulative investigation was performed and the exceptional properties of the atmospheric channels were numerically demonstrated. Among the interesting properties are the ability to guide light in a confined manner in a refractive channel, the ability to gather different sources to the same channel, and the ability to maintain a constant relative phase within the channel between several sources. The newly introduced guiding properties combined with a suggested method for channel probing and phase measurement by aerosol backscattered radiation allows coherence improvement of the phased array's elements and energy refocusing at the location of the channel in order to increase power in the bucket without feedback from the target. The method relies on the electronic focusing, electronic scanning, and time gating of the OPA, combined with elements of the relative phase measurements.

Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors.

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy using a special waveguide based on a silver halide fiber was used for probing the heat-induced secondary structure and conformation changes of bovine serum albumin (BSA). From the secondary derivative and the curve fitting of the obtained ATR-FTIR spectra, the changes of the BSA secondary structure with temperature were clearly identified. Two different thermal denaturation temperature ranges (i.e., 50-52 and 80-82 °C, at which a change of the protein structure occurred) were determined, while only one denaturation temperature was previously identified via classical FTIR measurements. Additionally, taking advantage of two-dimensional correlation spectroscopy more detailed information on changes of the protein secondary structure was revealed. The developed method facilitates in situ, sensitive, and more in-depth probing of protein secondary structures, which represents a significant advancement compared to conventional characterization methods.

Corneal cut closure using temperature-controlled CO2 laser soldering system.

We aimed to evaluate the effectiveness of temperature-controlled laser soldering for repair of large perforated corneas in a porcine model. Eight Yorkshire pigs aged 6 months underwent 6-mm-deep 180° crescent-shaped trephination of the central corneas. Right corneal injuries were repaired by placement of 47 % bovine albumin along the cut followed by CO2 laser soldering (power density 16 W/cm(2)) to a target temperature of 65(°). Left corneal injuries were repaired with 10/0 nylon sutures. The groups were compared for operative time, leakage, and histopathological findings. Mean tissue temperature was 63 ± 4 °C. Mean operative time was 31.57 ± 2.8 min in laser-soldered eyes and 41.38 ± 2.3 min in controls (p < 0.0001, unpaired Student's t test). Compared to controls, the soldered corneas had less neovascularization, complete re-epithelization, and mild stromal inflammation. There was no leakage in either group. Combined CO2 laser and radiometer is effective for the in vivo repair of corneal cuts. These results have important implications for modern corneal surgery. Further studies are needed in the clinical setting.

Perfectly correlated phase screen realization using sparse spectrum harmonic augmentation.

The split-step Fourier method is commonly used to simulate the propagation of radiation in a turbulent atmosphere using two-dimensional phase screens that have the desired spatial spectral content given by the atmospheric power spectrum. Using existing methodologies, isotropy of the structure function can never be achieved, mainly along the axis of propagation, for several reasons. In this paper, we introduce the sparse spectrum harmonic augmentation method that will address the lack of isotropy along the propagation axis, the limited achievable frequencies, and the limited time development possible using known approaches. Following the methodology described will produce phase screens that are transversely endless, perfectly correlated along the propagation axis, and contain the desired spectral content, including the low frequencies that even though they contain most of the energy, are usually neglected. The methodology presented can be used for many aspects of wave propagation in random media, such as atmospheric propagation, underwater acoustics, radio wave propagation in the ionosphere, and more.

CO2 laser welding of corneal cuts with albumin solder using radiometric temperature control.

PURPOSE: To examine the efficacy and reproducibility of CO2 laser soldering of corneal cuts using real-time infrared fiber-optic radiometric control of tissue temperature in bovine eyes (in vitro) and to evaluate the duration of this procedure in rabbit eyes (in vivo). METHODS: In vitro experiment: a 6-mm central perforating cut was induced in 40 fresh bovine eyes and sealed with a CO2 laser, with or without albumin soldering, following placement of a single approximating nylon suture. A fiber-optic radiometric temperature control system for the CO2 laser was used. Leaking pressure and histological findings were analyzed and compared between groups. In vivo experiment: following creation of a central perforation, 6 rabbit eyes were treated with a CO2 laser with albumin solder and 6 rabbit eyes were treated with 10-0 nylon sutures. The amount of time needed for completion of the procedures was compared. RESULTS: In vitro experiment: effective sealing was achieved by CO2 laser soldering. Mean (± SD) leaking pressure was 109 ± 30 mm Hg in the bovine corneas treated by the laser with albumin solder compared to 51 ± 7 mm Hg in the sutured control eyes (n = 10 each; p < 0.001). Mean leaking pressures were much lower in the corneal cuts sealed only with the laser without albumin solder (48 ± 12 mm Hg) and in the cuts sealed only with albumin without laser welding (6.3 ± 4 mm Hg) than in the cuts treated with laser welding and albumin solder. In vivo experiment: mean surgical time was 140 ± 17 s in the laser-treated rabbits compared to 330 ± 30 s in the sutured controls (n = 6; p < 0.001). A histopathological study of the rabbit corneas 1 day after laser soldering revealed sealed corneal edges with a small gap bridged by coagulated albumin. The inflammatory reaction was minimal in contrast to the sutured controls. No thermal damage was detected at the wound edges. CONCLUSIONS: CO2 laser soldering combined with the fiber-optic radiometer is an effective, reliable, and rapid tool for the closure of corneal wounds, and holds advantages over conventional suturing in terms of leaking pressure and surgical time.

Laser tissue soldering of the gastrointestinal tract: A systematic review LTS of the gastrointestinal tract.

Background: Laser Tissue Soldering (LTS) is a promising tissue bonding technique in which a solder is applied between the tissues and then irradiated by laser, causing it to solidify and form links with the tissue. Methods: A comprehensive systematic review summarizing the state of research of LTS in the gastrointestinal tract. Results: Most studies were conducted on large animal tissues, using liquid proteinaceous solder, and irradiated by a continuous wave laser at 808 nm. LTS can provide better sealing and burst pressure than conventional methods. The application of LTS on top of or in addition to sutures showed an impressive increase in burst pressures. LTS may decrease the inflammatory and foreign body reaction caused by sutures. Conclusions: LTS has strong potential to be applied in a clinical setting in leak prevention and in closure of gastrointestinal structures as an adjunct or additional anastomotic technology, decreasing leak rates, morbidity, and mortality.

Silver halide single-mode strip waveguides for the mid-infrared.

We report the demonstration of single-mode AgCl(x)Br(1-x) strip waveguides for the mid-infrared (MIR). The waveguides were made by the deposition of AgCl(x)Br(1-x) layers on top of a Si-SiO(2) substrate, followed by photolithographic and lift-off processing. The propagation losses of 20 dB/cm were measured at λ=10.6 µm using the cut-back method. The development of these waveguides is a crucial step toward realizing AgCl(x)Br(1-x) MIR integrated optical circuits, which would be used for applications such as chemical sensing and stellar interferometry.

Mid-infrared luminescence properties of Dy-doped silver halide crystals.

The absorption and the kinetics of the emission in the mid-infrared (mid-IR) were investigated in AgCl(x)Br(1-x) crystals doped with Dy(3+) ions. Strong emission bands were detected at 3, 4.4, and 5.5 µm and attributed to the (6)H(13/2)→(6)H(15/2), (6)H(11/2)→(6)H(13/2), and (6)F(11/2)+(6)H(9/2)→(6)H(11/2) transitions. Various optical parameters were calculated for the Dy(3+) doped crystals, using the Judd-Ofelt approximation and the rate equations. The measured results and the calculated parameters indicate that these doped crystals could be used for the development of mid-IR solid-state lasers or mid-IR fiber lasers.

Temperature-controlled two-wavelength laser soldering of tissues.

BACKGROUND AND OBJECTIVE: Laser tissue soldering is a method for bonding of incisions in tissues. A biological solder is spread over the cut, laser radiation heats the solder and the underlying cut edges and the incision is bonded. This method offers many advantages over conventional techniques (e.g., sutures). Past researches have shown that laser soldering, using a single laser, does not provide sufficient strength for bonding of cuts in thick (>1 mm) tissues. This study introduces a novel method for laser soldering of thick tissues, under temperature control, using two lasers, emitting two different wavelengths. STUDY DESIGN/MATERIALS AND METHODS: An experimental system was built, using two lasers: (i) a CO(2) laser, whose radiation heated the upper surface of the tissue and (ii) a GaAs laser that heated an albumin layer under the tissue. An infrared fiber-optic radiometer monitored the temperature of the tissue. All three devices were connected to a computer that controlled the process. A computer simulation was written to optimize the system parameters. The system was tested on tissue phantoms, to validate the simulation and ensure that both the upper and lower sides of the cut were heated, and that the temperature could be controlled on both sides. The system was then used ex vivo to bond longitudinal cuts of lengths ∼12 mm in the esophagi of large farm pigs. RESULTS: The theoretical simulations showed a good stabilization of the temperatures at the upper and lower tissue surfaces at the target values. Experiments on tissue phantom showed a good agreement with these simulations. Incisions in esophagi, removed from large farm pigs, were then successfully bonded. The mean burst pressure was ∼3.6 m of water. CONCLUSIONS: This study demonstrated the capability of soldering cuts in thick tissues, paving the way for new types of surgical applications.

Comparative study of mid-infrared fibers for modal filtering.

We compare the filtering capabilities of two infrared fibers developed to achieve a high rejection ratio of the higher order modes in order to obtain compact modal filters devoted to stellar interferometry. Two types of double-clad fibers are studied: a fiber with a second thin absorbing cladding and a fiber with a second thick absorbing cladding closer to the fiber core; both are single mode around the CO(2) band (10.6 µm). We present the single-mode spectral domain and the nulling capabilities of both fibers for different fiber lengths, comparing simulations with experimental results. We show that the filtering capabilities are improved when the absorbing clad is closer to the fiber core, as the propagation distance needed to filter out these modes is shorter. Thus, to obtain high rejection ratios in compact devices, an absorbing cladding close to the core of the fiber is compulsory in order to suppress cladding modes that could eventually recouple into the waveguide. We present an empirical model that allows determining the minimum filter length, considering only one effective leaky mode with low attenuation, which considerably simplifies the theoretical studies.

Temporal heating profile influence on the immediate bond strength following laser tissue soldering.

BACKGROUND: Bonding of tissues by laser heating is considered as a future alternative to sutures and staples. Increasing the post-operative bond strength remains a challenging issue for laser tissue bonding, especially in organs that have to sustain considerable tension or pressure. In this study, we investigated the influence of different temporal heating profiles on the strength of soldered incisions. The thermal damage following each heating procedure was quantified, in order to assess the effect of each heating profile on the thermal damage. MATERIALS AND METHODS: Incisions in porcine bowel tissue strips (1 cmx4 cm) were soldered, using a 44% liquid albumin mixed with indocyanine green and a temperature controlled laser (830 nm) tissue bonding system. Heating was done either with a linear or a step temporal heating profile. The incisions were bonded by soldering at three points, separated by 2 mm. Set-point temperatures of T(set) = 60, 70, 80, 90, 100, 110, 150 degrees C and dwell times of t(d) = 10, 20, 30, 40 seconds were investigated. The bond strength was measured immediately following each soldering by applying a gradually increased tension on the tissue edges until the bond break. RESULTS: Bonds formed by linear heating were stronger than the ones formed by step heating: at T(set) = 80 degrees C the bonds were 40% stronger and at T(set) = 90 degrees C the bonds strength was nearly doubled. The bond strength difference between the heating methods was larger as T(set) increased. CONCLUSION: Linear heating produced stronger bonds than step heating. The difference in the bond strength was more pronounced at high set-point temperatures and short dwell times. The bond strength could be increased with either higher set-point temperature or a longer dwell time.

Technical Note: Noninvasive mid-IR fiber-optic evanescent wave spectroscopy (FEWS) for early detection of skin cancers.

PURPOSE: Melanoma is the most lethal of the three primary skin cancers, including also basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), which are less lethal. The accepted diagnosis process involves manually observing a suspicious lesion through a Dermascope (i.e., a magnifying glass), followed by a biopsy. This process relies on the skill and the experience of a dermatologist. However, to the best of our knowledge, there is no accepted automatic, noninvasive, and rapid method for the early detection of the three types of skin cancer, distinguishing between them and noncancerous lesions, and identifying each of them. It is our aim to develop such a system. METHODS: We developed a fiber-optic evanescent wave spectroscopy (FEWS) system based on middle infrared (mid-IR) transmitting AgClBr fibers and a Fourier-transform infrared spectrometer (FTIR). We used the system to perform mid-IR spectral measurements on suspicious lesions in 90 patients, before biopsy, in situ, and in real time. The lesions were then biopsied and sent for pathology. The spectra were analyzed and the differences between pathological and healthy tissues were found and correlated. RESULTS: Five of the lesions measured were identified as melanomas, seven as BCC, and three as SCC. Using mathematical analyses of the spectra of these lesions we were able to tell that all were skin cancers and we found specific and easily identifiable differences between them. CONCLUSIONS: This FEWS method lends itself to rapid, automatic and noninvasive early detection and characterization of skin cancers. It will be easily implemented in community clinics and has the potential to greatly simplify the diagnosis process.

Improved method for estimating the minimum length of modal filters fabricated for stellar interferometry.

We present an improved theoretical model to estimate the minimum fiber length required for achieving a desired degree of wavefront filtering in stellar interferometry. The proposed model is based on modal analysis of the fiber and is compared with numerical results obtained through the beam propagation method as well as with reported experimental observations. We also study the effect of introducing a spatial filter at the output end of the fiber and show that the required fiber length can be reduced significantly by introducing a circular aperture of optimum radius after the fiber.

Single mode mid-infrared silver halide asymmetric flat waveguide obtained from crystal extrusion.

A flat waveguide for the middle infrared was made by co-extrusion of two silver halide crystals of different chemical compositions. The transmission of the waveguide and its modal behavior was studied using a Fourier Transform Spectrometer and a dedicated optical bench. Analyzing this spectrum, we were able to obtain the cut-off wavelength of the waveguide. We observed a single mode behavior for wavelengths longer than 8.83mum, in good agreement with the theoretically expected values. This novel procedure is ideal for tailoring the properties of the waveguide for specific applications, in particular the spectral range where it exhibits a single-mode behavior. It can thus be applied to achieve modal filtering for mid-IR astronomical interferometers (e.g. beam combiners, nullers, etc.).

In vitro large diameter bowel anastomosis using a temperature controlled laser tissue soldering system and albumin stent.

BACKGROUND AND OBJECTIVE: In today's age of advancing surgical technology, there is a need for better and simpler methods of tissue bonding. The use of lasers for tissue welding or soldering is one of these sutureless methods. In 30 years of laser tissue bonding (LTB) research, published reports of cylindrical organ anastomosis were limited to small diameters. The tension resisted by the anastomosis, which is caused by the intraluminal pressure, is also proportional to the organ diameter. Therefore the anastomosis of large diameter organs requires significantly stronger mechanical strength. The aim of this study is to demonstrate such an anastomosis. MATERIALS AND METHODS: In vitro anastomosis of porcine small bowel was performed by either LTB or sutures. Anastomosis in the laser group (number of samples, n = 15) included two main stages of soldering. The bowel edges were approximated over a solid albumin stent and heated with a temperature controlled GaAs laser system to 75 degrees C. This was followed by spreading liquid albumin on the anastomotic line and heating by the same system again to 75 degrees C. The control group (n = 5) was sutured anastomosis. All anastomoses were assessed by burst pressure measurement. RESULTS: The burst pressure of the laser group attained 170+/-40 mmHg which was significantly higher than that of the sutured group 83+/-37 mmHg (P < 0.001). CONCLUSION: This report describes the in vitro LTB anastomosis of a large diameter cylindrical organ. The immediate bond strength, as estimated by burst pressure measurements, was double compared to sutured anastomosis.

Laser-induced hyperthermia for treatment of granulation tissue growth in rats.

OBJECTIVE: We aimed to develop a new technique for treatment of granulation tissue (GT) growth using local hyperthermia. METHODS: A temperature-controlled diode laser system was developed for induction of mild hyperthermia in real time. GT was generated by harvesting the skin over the gluteal fascia in rats. Histopathological analysis was used to estimate the effect of hyperthermia on the tissue. RESULTS: In untreated rats, GT was detected within 3 days and reached maximal thickness after 12 days. Hyperthermia at 43 degrees C and above significantly decreased GT thickness (n = 8 per group). Hyperthermia at 48 degrees C for 3 minutes was the most efficient parameter for treatment of GT (51% reduction), with minimal (5%) muscle necrosis. CONCLUSIONS: Hyperthermia can significantly inhibit GT growth, with minimal damage to surrounding structures. Our findings suggest a possible role for hyperthermia as a therapeutic model against GT. Further research and long-term studies are needed to explore the utility of laser-induced hyperthermia for inhibition of GT growth.

Laser Soldering of Cartilage Graft Interposed Into a Tracheal Incision in a Porcine Model.

OBJECTIVES/HYPOTHESIS: Investigate the feasibility of soldering a free cartilage graft into a tracheal defect by laser heating and assessing the resulting burst pressure and thermal damage to the cartilage. STUDY DESIGN: Animal study. METHODS: A 20 × 8 mm defect was created in fresh cadaveric pig tracheas, a cartilage graft of the same size was harvested from the thyroid ala cartilage, and the graft was fitted into the defect. The soldering process involved covering the edges with liquid albumin and using a fiber-laser system for heating the edges to temperature T under temperature control. This was done for groups of grafts at various temperatures T = 60°C to 90°C. The tracheas were sealed, for each group the burst pressure was measured, and a histologic examination of the soldered incisions was performed. RESULTS: The burst pressures were in the range of 66 to 409 mm Hg. The median burst pressure was 78, 157, 231, and 146 mm Hg, respectively, for T = 60°C, 70°C, 80°C, and 90°C. Statistical analysis revealed significant differences in burst pressures between the T = 60°C group and the T = 80°C and T = 90°C groups (P < .05). The highest burst pressure was measured in the T = 80°C group. Histologic examination revealed no thermal damage to the cartilage at this temperature. CONCLUSIONS: Performing a sutureless laser soldering of a free cartilage graft to a tracheal defect, achieving an immediate watertight bond, is feasible. At T = 80°C the highest burst pressures were achieved. No histologic damage was observed. In vivo studies are needed before implementation of this technique in laryngotracheopasty. LEVEL OF EVIDENCE: NA Laryngoscope, 129:58-62, 2019.

Mid-IR evanescent-field fiber sensor with enhanced sensitivity for volatile organic compounds.

The increasing awareness of the harsh environmental and health risks associated with air pollution has placed volatile organic compounds (VOCs) sensor technologies in elevated demand. While the currently available VOC-monitoring technologies are either bulky and expensive, or only capable of measuring a total VOC concentration, the selective detection of VOCs in the gas-phase remains a challenge. To overcome this, a novel method and device based on mid-IR evanescent-wave fiber-optic spectroscopy, which enables enhanced detection of VOCs, is hereby proposed. This is achieved by increasing the number of analyte molecules in the proximity of the evanescent field via capillary condensation inside nano-porous microparticles coated on the fiber surface. The nano-porous structure of the coating allows the VOC analytes to rapidly diffuse into the pores and become concentrated at the surface of the fiber, thereby allowing the utilization of highly sensitive evanescent-wave spectroscopy. To ascertain the effectiveness and performance of the sensor, different VOCs are measured, and the enhanced sensitivity is analyzed using a custom-built gas cell. According to the results presented here, our VOC sensor shows a significantly increased sensitivity compared to that of an uncoated fiber.