Cell Membrane Oscillations under Radiofrequency Electromagnetic Modulation.

Cell responses to external radiofrequencies (RF) are a fundamental problem of much scientific research, clinical applications, and even daily lives surrounded by wireless communication hardware. In this work, we report an unexpected observation that the cell membrane can oscillate at the nanometer scale in phase with the external RF radiation from kHz to GHz. By analyzing the oscillation modes, we reveal the mechanism behind the membrane oscillation resonance, membrane blebbing, the resulting cell death, and the selectivity of plasma-based cancer treatment based on the difference in the membrane's natural frequencies among cell lines. Therefore, a selectivity of treatment can be achieved by aiming at the natural frequency of the target cell line to focus the membrane damage on the cancer cells and avoid normal tissues nearby. This gives a promising cancer therapy that is especially effective in the mixing lesion of the cancer cells and normal cells such as glioblastoma where surgical removal is not applicable. Along with these new phenomena, this work provides a general understanding of the cell coupling with RF radiation from the externally stimulated membrane behavior to the cell apoptosis and necrosis.

Nanosynthesis by atmospheric arc discharges excited with pulsed-DC power: a review.

Plasma technology is actively used for nanoparticle synthesis and modification. All plasma techniques share the ambition of providing high quality, nanostructured materials with full control over their crystalline state and functional properties. Pulsed-DC physical/chemical vapour deposition, high power impulse magnetron sputtering, and pulsed cathodic arc are consolidated low-temperature plasma processes for the synthesis of high-quality nanocomposite films in vacuum environment. However, atmospheric arc discharge stands out thanks to the high throughput, wide variety, and excellent quality of obtained stand-alone nanomaterials, mainly core-shell nanoparticles, transition metal dichalcogenide monolayers, and carbon-based nanostructures, like graphene and carbon nanotubes. Unique capabilities of this arc technique are due to its flexibility and wide range of plasma parameters achievable by modulation of the frequency, duty cycle, and amplitude of pulse waveform. The many possibilities offered by pulsed arc discharges applied on synthesis of low-dimensional materials are reviewed here. Periodical variations in temperature and density of the pulsing arc plasma enable nanosynthesis with a more rational use of the supplied power. Parameters such as plasma composition, consumed power, process stability, material properties, and economical aspects, are discussed. Finally, a brief outlook towards future tendencies of nanomaterial preparation is proposed. Atmospheric pulsed arcs constitute promising, clean processes providing ecological and sustainable development in the production of nanomaterials both in industry and research laboratories.

Canady Cold Helios Plasma Reduces Soft Tissue Sarcoma Viability by Inhibiting Proliferation, Disrupting Cell Cycle, and Inducing Apoptosis: A Preliminary Report.

Soft tissue sarcomas (STS) are a rare and highly heterogeneous group of solid tumors, originating from various types of connective tissue. Complete removal of STS by surgery is challenging due to the anatomical location of the tumor, which results in tumor recurrence. Additionally, current polychemotherapeutic regimens are highly toxic with no rational survival benefit. Cold atmospheric plasma (CAP) is a novel technology that has demonstrated immense cancer therapeutic potential. Canady Cold Helios Plasma (CHCP) is a device that sprays CAP along the surgical margins to eradicate residual cancer cells after tumor resection. This preliminary study was conducted in vitro prior to in vivo testing in a humanitarian compassionate use case study and an FDA-approved phase 1 clinical trial (IDE G190165). In this study, the authors evaluate the efficacy of CHCP across multiple STS cell lines. CHCP treatment reduced the viability of four different STS cell lines (i.e., fibrosarcoma, synovial sarcoma, rhabdomyosarcoma, and liposarcoma) in a dose-dependent manner by inhibiting proliferation, disrupting cell cycle, and inducing apoptosis-like cell death.

Anti-Melanoma Capability of Contactless Cold Atmospheric Plasma Treatment.

In this study, we demonstrated that the widely used cold atmospheric plasma (CAP) jet could significantly inhibit the growth of melanoma cells using a contactless treatment method, The flow rate of helium gas was a key operational parameter to modulate electromagnetic (EM) effect on melanoma cells. Metal sheets with different sizes could be used as a strategy to control the strength of EM effect. More attractive, the EM effect from CAP could penetrate glass/polystyrene barriers as thick as 7 mm. All these discoveries presented the profound non-invasive nature of a physically based CAP treatment, which provided a solid foundation for CAP-based cutaneous/subcutaneous tumor therapy.

Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA.

Breast cancer is the most common cancer among women worldwide. Its molecular receptor marker status and mutational subtypes complicate clinical therapies. Cold atmospheric plasma is a promising adjuvant therapy to selectively combat many cancers, including breast cancer, but not normal tissue; however, the underlying mechanisms remain unexplored. Here, four breast cancer cell lines with different marker status were treated with Canady Helios Cold Plasma™ (CHCP) at various dosages and their differential progress of apoptosis was monitored. Inhibition of cell proliferation, induction of apoptosis, and disruption of the cell cycle were observed. At least 16 histone mRNA types were oxidized and degraded immediately after CHCP treatment by 8-oxoguanine (8-oxoG) modification. The expression of DNA damage response genes was up-regulated 12 h post-treatment, indicating that 8-oxoG modification and degradation of histone mRNA during the early S phase of the cell cycle, rather than DNA damage, is the primary cause of cancer cell death induced by CHCP. Our report demonstrates for the first time that CHCP effectively induces cell death in breast cancer regardless of subtyping, through histone mRNA oxidation and degradation during the early S phase of the cell cycle.

Enhancing cold atmospheric plasma treatment of cancer cells by static magnetic field.

It has been reported since late 1970 that magnetic field interacts strongly with biological systems. Cold atmospheric plasma (CAP) has also been widely studied over the past few decades in physics, biology, and medicine. In this study, we propose a novel idea to combine static magnetic field (SMF) with CAP as a tool for cancer therapy. Breast cancer cells and wild type fibroblasts were cultured in 96-well plates and treated by CAP with or without SMF. Breast cancer cells MDA-MB-231 showed a significant decrease in viability after direct plasma treatment with SMF (compared to only plasma treatment). In addition, cancer cells treated by the CAP-SMF-activated medium (indirect treatment) also showed viability decrease but was slightly weaker than the direct plasma-SMF treatment. By integrating the use of SMF and CAP, we were able to discover their advantages that have yet to be utilized. Bioelectromagnetics. 38:53-62, 2017. © 2016 Wiley Periodicals, Inc.

Electromagnetic Nature of Distant Interaction of the Atmospheric Pressure Helium Plasma Discharge Tube with Glioblastoma Cancer Cells.

Atmospheric pressure coaxial gaseous discharge tubes (DTs) with helium have demonstrated potential for in vitro inactivation or sensitization of glioblastoma cancer cells. Here, we study the effect of two configurations of the DT electrode system on its electromagnetic emissivity as well as other physical factors (heating and UV emission) that form in the vicinity of this device. We demonstrate that the configuration of the DT electrodes that concentrates the discharge streamers near the top of the device has a distant (cm scale) deactivation effect on U87-MG glioblastoma cancer cells when irradiated, without measurable UV components in the DT optical emission spectra. This effect persists even through different barriers such as glass, plastic, or quartz Petri dishes but is eliminated when glass or plastic dishes are filled with water. These findings demonstrate the potential for development of noninvasive, physical-based treatment methods of deep-tissue tumors.

Enhanced human bone marrow mesenchymal stem cell functions in novel 3D cartilage scaffolds with hydrogen treated multi-walled carbon nanotubes.

Cartilage tissue is a nanostructured tissue which is notoriously hard to regenerate due to its extremely poor inherent regenerative capacity and complex stratified architecture. Current treatment methods are highly invasive and may have many complications. Thus, the goal of this work is to use nanomaterials and nano/microfabrication methods to create novel biologically inspired tissue engineered cartilage scaffolds to facilitate human bone marrow mesenchymal stem cell (MSC) chondrogenesis. To this end we utilized electrospinning to design and fabricate a series of novel 3D biomimetic nanostructured scaffolds based on hydrogen (H2) treated multi-walled carbon nanotubes (MWCNTs) and biocompatible poly(L-lactic acid) (PLLA) polymers. Specifically, a series of electrospun fibrous PLLA scaffolds with controlled fiber dimension were fabricated in this study. In vitro MSC studies showed that stem cells prefer to attach in the scaffolds with smaller fiber diameter. More importantly, the MWCNT embedded scaffolds showed a drastic increase in mechanical strength and a compressive Young's modulus matching to natural cartilage. Furthermore, our MSC differentiation results demonstrated that incorporation of the H2 treated carbon nanotubes and poly-L-lysine coating can induce more chondrogenic differentiations of MSCs than controls. After two weeks of culture, PLLA scaffolds with H2 treated MWCNTs and poly-L-lysine can achieve the highest glycosaminoglycan synthesis, making them promising for further exploration for cartilage regeneration.

Data on a two-dimensional Pd-cellulose with optimized morphology for the effective solar to steam generation.

The data presented in this article are related to the research article entitled "Two-dimensional Pd-cellulose with optimized morphology for the effective solar to steam generation (Omelianovych et al., Desalination, 535, 115820 (2023)). We provide complementary analysis of the plasma synthesis parameters, such as plasma power optimization, which were omitted in the original research. The SEM images, XRD micrographs, XPS spectra, and evaporation performance of various plasma-synthesized Pd-cellulose absorbers are presented.

Data on a high electrocatalytic activity of metal-WO3 nanocomposite electrocatalysts for hydrogen evolution reaction.

The data given in this article are related to the research article entitled "High electrocatalytic activity of Rh-WO3 electrocatalyst for hydrogen evolution reaction under the acidic, alkaline, and alkaline seawater electrolytes (N.-A. Nguyen et al., 2023) [1]. In this work, metal-WO3 nanocomposites were synthesized and used as electrocatalysts for hydrogen evolution reaction (HER) performance. The morphology and chemical properties of the prepared metal-WO3 nanocomposites were investigated by using scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) techniques.

The First Cold Atmospheric Plasma Phase I Clinical Trial for the Treatment of Advanced Solid Tumors: A Novel Treatment Arm for Cancer.

Local regional recurrence (LRR) remains the primary cause of treatment failure in solid tumors despite advancements in cancer therapies. Canady Helios Cold Plasma (CHCP) is a novel Cold Atmospheric Plasma device that generates an Electromagnetic Field and Reactive Oxygen and Nitrogen Species to induce cancer cell death. In the first FDA-approved Phase I trial (March 2020-April 2021), 20 patients with stage IV or recurrent solid tumors underwent surgical resection combined with intra-operative CHCP treatment. Safety was the primary endpoint; secondary endpoints were non-LRR, survival, cancer cell death, and the preservation of surrounding healthy tissue. CHCP did not impact intraoperative physiological data (p > 0.05) or cause any related adverse events. Overall response rates at 26 months for R0 and R0 with microscopic positive margin (R0-MPM) patients were 69% (95% CI, 19-40%) and 100% (95% CI, 100-100.0%), respectively. Survival rates for R0 (n = 7), R0-MPM (n = 5), R1 (n = 6), and R2 (n = 2) patients at 28 months were 86%, 40%, 67%, and 0%, respectively. The cumulative overall survival rate was 24% at 31 months (n = 20, 95% CI, 5.3-100.0). CHCP treatment combined with surgery is safe, selective towards cancer, and demonstrates exceptional LRR control in R0 and R0-MPM patients. (Clinical Trials identifier: NCT04267575).

Greater osteoblast and mesenchymal stem cell adhesion and proliferation on titanium with hydrothermally treated nanocrystalline hydroxyapatite/magnetically treated carbon nanotubes.

With an increasingly active and aging population, a growing number of orthopedic procedures are performed annually. However, traditional orthopedic implants face many complications such as infection, implant loosening, and poor host tissue integration leading to implant failure. Metal implant materials such as titanium and its alloys are widely used in orthopedic applications mainly based on their excellent mechanical properties and biological inertness. Since human bone extracellular matrix is nanometer in dimension comprised of rich nanostructured hydroxyapatite particles and collagen nanofibers, it is highly desirable to design a biologically-inspired nanostructured coating which renders the biocompatible titanium surface into a biomimetic and bioactive interface, thus enhancing osteoblast adhesion and promoting osseointegration. For this purpose, a biomimetic nanostructured coating based on nanocrystalline hydroxyapatite and single wall carbon nanotubes was designed. Specifically, nano hydroxyapatites with good crystallinity and biomimetic dimensions were prepared via a wet chemistry method and hydrothermal treatment. Microcrystalline hydroxyapatite with larger grain sizes can be obtained without hydrothermal treatment. The carbon nanotubes with different diameter and length were synthesized via an arc plasma method in the presence or absence of a magnetic field. Transmission electron microscopy images illustrate the regular, rod-like nanocrystalline and biomimetic nanostructure of hydrothermally treated nano hydroxyapatite. In addition, the length of carbon nanotubes can be significantly increased under external magnetic fields when compared to nanotubes produced without a magnetic field. More importantly, the in vitro study demonstrated for the first time that osteoblast and mesenchymal stem cell adhesion and proliferation were greater on titanium with hydrothermally treated nanocrystalline hydroxyapatites/magnetically treated carbon nanotubes, which suggests the potential of these novel nanostructured materials for orthopedic applications.

Preclinical Cold Atmospheric Plasma Cancer Treatment.

CAP is an ionized gas generated under atmospheric pressure conditions. Due to its reactive chemical components and near-room temperature nature, CAP has promising applications in diverse branches of medicine, including microorganism sterilization, biofilm inactivation, wound healing, and cancer therapy. Currently, hundreds of in vitro demonstrations of CAP-based cancer treatments have been reported. However, preclinical studies, particularly in vivo studies, are pivotal to achieving a final clinical application. Here, we comprehensively introduced the research status of the preclinical usage of CAP in cancer treatment, by primarily focusing on the in vivo studies over the past decade. We summarized the primary research strategies in preclinical and clinical studies, including transdermal CAP treatment, post-surgical CAP treatment, CAP-activated solutions treatment, and sensitization treatment to drugs. Finally, the underlying mechanism was discussed based on the latest understanding.

Theranostic Potential of Adaptive Cold Atmospheric Plasma with Temozolomide to Checkmate Glioblastoma: An In Vitro Study.

Cold atmospheric plasma (CAP) has been used for the treatment of various cancers. The anti-cancer properties of CAP are mainly due to the reactive species generated from it. Here, we analyze the efficacy of CAP in combination with temozolomide (TMZ) in two different human glioblastoma cell lines, T98G and A172, in vitro using various conditions. We also establish an optimized dose of the co-treatment to study potential sensitization in TMZ-resistant cells. The removal of cell culture media after CAP treatment did not affect the sensitivity of CAP to cancer cells. However, keeping the CAP-treated media for a shorter time helped in the slight proliferation of T98G cells, while keeping the same media for longer durations resulted in a decrease in its survivability. This could be a potential reason for the sensitization of the cells in combination treatment. Co-treatment effectively increased the lactate dehydrogenase (LDH) activity, indicating cytotoxicity. Furthermore, apoptosis and caspase-3 activity also significantly increased in both cell lines, implying the anticancer nature of the combination. The microscopic analysis of the cells post-treatment indicated nuclear fragmentation, and caspase activity demonstrated apoptosis. Therefore, a combination treatment of CAP and TMZ may be a potent therapeutic modality to treat glioblastoma. This could also indicate that a pre-treatment with CAP causes the cells to be more sensitive to chemotherapy treatment.

Demonstration of electric micropropulsion multimodality.

Electric propulsion has become popular nowadays owing to the trend of miniaturizing the size and mass of satellites. However, the main drawback of the most popular approach-Hall thrusters-is that their efficiency and thrust-to-power ratio (TPR) markedly deteriorate when its size and power level are reduced. Here, we demonstrate an alternative approach-a minute low-power (<50 W), lightweight (~100 g), two-stage propulsion system. The system is based on a micro-cathode vacuum arc thruster with magnetoplasmadynamic second stage (µCAT-MPD), which achieves the following parameters: a thrust of up to 1.7 mN at a TPR of 37 µN/W and an efficiency of ~50%. A µCAT-MPD system, in addition to "traditional" inverse, displays the anomalous direct (growing) "TPR versus specific impulse Isp" trend at high Isp values and allows multimodality at high efficiency.

Cold Plasma Discharge Tube Enhances Antitumoral Efficacy of Temozolomide.

Glioblastoma (GBM) is a fatal human brain tumor with a low survival rate. Temozolomide (TMZ) has been widely used in GBM therapy with noticeable side effects. Cold plasma is an ionized gas that is generated near room temperature. Here, we demonstrated the enhancement therapeutic efficacy of TMZ via using a cold plasma source based on nonequilibrium plasma in a sealed glass tube, named a radial cold plasma discharge tube (PDT). The PDT affected glioblastoma cells' function just by its electromagnetic (EM) emission rather than any chemical factors in the plasma. The PDT selectively increased the cytotoxicity of TMZ on two typical glioblastoma cell lines, U87MG and A172, compared with normal astrocyte cell line hTERT/E6/E7 to some extent. Furthermore, on the basis of a patient-derived xenograft model, our preliminary in vivo studies demonstrated the drastically improved mean survival days of the tumor-barrier mice by more than 100% compared to control. The PDT is not only independent of continuous helium supply but is also capable of resisting the interference of environmental changes. Thus, the PDT was a stable and low-cost cold atmospheric plasma source. In short, this study is the first to demonstrate the promising application of PDTs in GBM therapy as a noninvasive and portable modality.

BCL2A1 regulates Canady Helios Cold Plasma-induced cell death in triple-negative breast cancer.

Breast cancer is the leading cause of cancer death among women. Triple-negative breast cancer (TNBC) has a poor prognosis and frequently relapses early compared with other subtypes. The Cold Atmospheric Plasma (CAP) is a promising therapy for prognostically poor breast cancer such as TNBC. The Canady Helios Cold Plasma (CHCP) induces cell death in the TNBC cell line without thermal damage, however, the mechanism of cell death by CAP treatment is ambiguous and the mechanism of resistance to cell death in some subset of cells has not been addressed. We investigate the expression profile of 48 apoptotic and 35 oxidative gene markers after CHCP treatment in six different types of breast cancer cell lines including luminal A (ER+ PR+/-HER2-), luminal B (ER+PR+/-HER2+), (ER-PR-HER2+), basal-like: ER-PR-HER2- cells were tested with CHCP at different power settings and at 4 different incubation time. The expression levels of the gene markers were determined at 4 different intervals after the treatment. The protein expression of BCL2A1 was only induced after CHCP treatment in TNBC cell lines (p < 0.01), whereas the HER2-positive and ER, PR positive cell lines showed little or no expression of BCL2A1. The BCL2A1 and TNF-alpha expression levels showed a significant correlation within TNBC cell lines (p < 0.01). Silencing BCL2A1 mRNA by siRNA increased the potency of the CHCP treatment. A Combination of CHCP and CPI203, a BET bromodomain inhibitor, and a BCL2A1 antagonist increased the CHCP-induced cell death (p < 0.05). Our results revealed that BCL2A1 is a key gene for resistance during CHCP induced cell death. This resistance in TNBCs could be reversed with a combination of siRNA or BCL2A1 antagonist-CHCP therapy.

Controlling diameter distribution of catalyst nanoparticles in arc discharge.

It is demonstrated that the diameter distribution of catalyst nanoparticles in arc discharge can be controlled by a magnetic field. The magnetic field affects the arc shape, shortens the diffusing time of the catalyst nanoparticles through the nucleation zone, and consequentially reduces the average diameters of nanoparticles. The average diameter is reduced from about 7.5 nm without magnetic field to about 5 nm is the case of a magnetic field. Decrease of the catalyst nanoparticle diameter with magnetic field correlates well with decrease in the single-wall carbon nanotube and their bundles diameters.

Non-thermal plasma multi-jet platform based on a flexible matrix.

A new plasma source design that merges the main characteristics of capacitive dielectric barrier discharge (DBD) and cold atmospheric plasma jet (CAPJ) is discussed. The DBD system contains a flexible, porous matrix consisting of silica aerogel, which is comprised between two biased electrodes. The helium flow supply subjected to a sinusoidal voltage of around 5 kV in amplitude and 15 kHz in frequency provides a set of plasma jets that propagates more than 1 cm beyond the active DBD region. The studied plasma multi-jet system consists of an array of three aligned jets that flow in the laminar regime, and it is intended for treating the surfaces of 3D objects and large areas. CAPJ performance is discussed as a hypothetical morphing source in flat and bent configurations. Electrical characterization and optical emission spectroscopy diagnostics have provided current-voltage waveforms and the composition of the CAPJ through the aerogel layer, respectively. This novel source is promising for biomedical applications that require full adaptation of plasma parameters to delicate samples, such as wound healing and treatment of surgical margins in plasma-based cancer surgery.