Prevalence of antibodies against severe fever with thrombocytopenia syndrome virus in shelter dogs in the Republic of Korea.

The purpose of this study was to investigate the seroprevalence of antibodies against severe fever with thrombocytopenia syndrome virus (SFTSV) in shelter dogs in the Republic of Korea (ROK) using an indirect immunofluorescence assay and virus neutralization test. Sera were collected from 426 dogs in 12 animal shelters throughout the ROK from March 2016 to November 2016. Overall, 59 of 426 (13.9%) samples were seropositive for antibodies against SFTSV. A significant difference was observed in accordance with the sampling region (p<0.001), but not according to the sex (p=0.279) or breed (p=0.729) of the dogs. The seroprevalence of SFTSV showed an inversely proportional trend to the latitude of the sampling regions: the highest rate was observed in the southern region followed by the Jeju-do region. This is the first report on the nationwide prevalence of antibodies against SFTSV in companion dogs in animal shelters throughout the ROK.

Molecular detection and phylogenetic analysis of severe fever with thrombocytopenia syndrome virus in shelter dogs and cats in the Republic of Korea.

Severe fever with thrombocytopenia syndrome is a tick-borne infectious disease. The present study investigated the prevalence of severe fever with thrombocytopenia syndrome virus (SFTSV) in shelter dogs and cats in the Republic of Korea (ROK). Blood samples were collected from 426 dogs and 215 cats in animal shelters throughout the ROK in 2016. Of the tested samples, one (0.2%) dog and one (0.5%) cat were positive for SFTSV. Phylogenetic analysis of the sequences obtained in the present study showed that the viruses belonged to the J3 clade, which is considered the dominant clade in the ROK. This study reports the first molecular detection of SFTSV in shelter dogs and cats in the ROK.

First molecular detection and phylogenetic analysis of Anaplasma phagocytophilum in shelter dogs in Seoul, Korea.

In this study, the status of Anaplasma phagocytophilum infection was assessed in shelter dogs in Seoul, Korea, with PCR and phylogenetic analyses. Nested PCR on 1058 collected blood samples revealed only one A. phagocytophilum positive sample (female, age <1year, mixed breed, collected from the north of the Han River). The genetic variability of A. phagocytophilum was evaluated by genotyping, using the 16S rRNA, groEL, and msp2 gene sequences of the positive sample. BLASTn analysis revealed that the 16S rRNA, groEL, and msp2 genes had 99.6%, 99.9%, and 100% identity with the following sequences deposited in GenBank: a cat 16S rRNA sequence from Korea (KR021166), a rat groEL sequence from Korea (KT220194), and a water deer msp2 sequence from Korea (HM752099), respectively. Phylogenetic analyses classified the groEL gene into two distinct groups (serine and alanine), whereas the msp2 gene showed a general classification into two groups (USA and Europe) that were further subgrouped according to region. To the best of our knowledge, this study is the first to describe the molecular diagnosis of A. phagocytophilum in dogs reared in Korea. In addition, the high genetic identity of the 16S rRNA and groEL sequences between humans and dogs from the same region suggests a possible epidemiological relation. Given the conditions of climate change, tick ecology, and recent incidence of human granulocytic anaplasmosis in Korea, the findings of this study underscore the need to establish appropriate control programs for tick-borne diseases in Korea.

First molecular detection and genetic analysis of Anaplasma phagocytophilum in shelter cats in Seoul, Korea.

Here, we report the molecular detection of Anaplasma phagocytophilum in shelter cats in Korea and the relationships between A. phagocytophilum gene sequences and the pathogenicity, region, and host specificity of this bacterium. Two (0.9%) out of 222 shelter cats from Seoul, Korea, yielded positive results for the A. phagocytophilum 16S rRNA, groEL, and msp2 genes. Phylogenetic analysis divided groEL gene sequences into two groups (alanine and serine), based on their nucleotide and amino acid sequences. A. phagocytophilum msp2 gene sequences were grouped per the region of isolation (Europe vs. USA, including Korea). Some nucleotide and amino acid sequences of groEL and msp2 showed distinctive patterns according to the region of isolation, which helped in distinguishing A. phagocytophilum gene sequences detected in Korea from those detected in the USA and Europe. Although the limited number of clinical anaplasmosis cases caused by A. phagocytophilum belonging to the alanine group prevents any firm conclusions, the results of the present study tend to refute the previous view that the pathogenicity of A. phagocytophilum is associated with the serine group. Moreover, our results suggest that genetic analyses of groEL and msp2 can be used to obtain a regional fingerprint of A. phagocytophilum.

Prevalence of Toxoplasma gondii infection in feral cats in Seoul, Korea.

The present study assessed the prevalence of Toxoplasma gondii infection in feral cat populations in Seoul using enzyme-linked immunosorbent assay (ELISA) and nested polymerase chain reaction (PCR). A total of 456 feral cats from 17 wards in Seoul was surveyed. The overall prevalence of T. gondii infection was 15.8% (69/456) by ELISA and 17.5% (80/456) by PCR; by gender, 17% (44/259) by ELISA and 16.2% (42/259) by PCR in males and 14.3% (28/196) by ELISA and 19.4% (38/196) by PCR in females. On a baseline of the Han River, prevalence was 15.1% (29/192) by ELISA and 15.6% (30/192) by PCR in the upper region and 16.4% (43/264) by ELISA and 18.9% (50/264) by PCR in the lower area. This suggested that toxoplasmosis is widespread throughout Seoul's feral cat population and it is critical that the city institute policies for the control of the feral cat population to reduce the risk of toxoplasmosis transmission to animals, including humans.