Differentiation of Body Fluid Stains Using a Portable, Low-Cost Ion Mobility Spectrometry Device-A Pilot Study.

The identification and recovery of suspected human biofluid evidence can present a bottleneck in the crime scene investigation workflow. Crime Scene Investigators typically deploy one of a number of presumptive enhancement reagents, depending on what they perceive an analyte to be; the selection of this reagent is largely based on the context of suspected evidence and their professional experience. Positively identified samples are then recovered to a forensic laboratory where confirmatory testing is carried out by large lab-based instruments, such as through mass-spectrometry-based techniques. This work proposes a proof-of-concept study into the use of a small, robust and portable ion mobility spectrometry device that can analyse samples in situ, detecting, identifying and discriminating commonly encountered body fluids from interferences. This analysis exploits the detection and identification of characteristic volatile organic compounds generated by gentle heating, at ambient temperature and pressure, and categorises samples using machine learning, providing investigators with instant identification. The device is shown to be capable of producing characteristic mobility spectra using a dual micro disc pump configuration which separates blood and urine from three visually similar interferences using an unsupervised PCA model with no misclassified samples. The device has the potential to reduce the need for potentially contaminating and destructive presumptive tests, and address the bottleneck created by the time-consuming and laborious detection, recovery and analysis workflow currently employed.

The importance of dark adaptation for forensic examinations; an evaluation of the Crime-lite Eye™.

Forensic practitioners are recommended to dark adapt their eyes prior to conducting evidential searches in the dark. The dark adaptation process remains poorly standardised across the discipline, with little quantified regarding the benefits of such preparative steps. Herein, we report the findings of a study that recruited 50 participants to assess the effectiveness of the Crime-lite Eye™, a darkness adaptation device developed to assist forensic practitioners both in the laboratory and in field. Participants were tasked with searching for the fluorescent signatures left by reaction of 1,8-diazafluoren-9-one (DFO) with amino acids, in a manner akin to the fluorogenic fingerprint treatment of porous evidence. Using an Epson Stylus Photo R265 inkjet printer, ink cartridges were filled with alanine solutions of various concentrations, allowing different motifs to be printed onto copy paper and subsequently developed using DFO. Participants searched for this 'evidence' both with and without dark adapted vision. On average, participants were able to locate and correctly recognise 16% more evidence once dark adapted using the Crime-lite Eye™. The increase in evidence located by participants once dark adapted suggests that crime scene officers should be dark adapting in order to visualise as much as possible. The time taken to dark adapt, 10 min on average during this study, is not excessively long, and should not significantly slow the investigation.

The laboratory perspective: Confirming the integrity of fingermark enhancement reagents.

Validation is particularly important in forensic science. Each process utilised by a forensic laboratory will undergo validation to ensure it is functioning as expected. Some pieces of equipment (hardware) will be simple to validate, and this will be conducted regularly. However, many commonly employed fingermark enhancement reagents, such as ninhydrin and 1,2-Indandione, do not have clearly defined and robust validation parameters. To that end, artificial fingermark solutions, particularly amino acid solutions, have previously been investigated as a controlled validation method, via deposition onto a substrate. Whilst amino acid reagents are important, there is also a wider group of latent fingermark constituents (targets) that require the use of other enhancement reagents within the forensic laboratory. The work presented herein seeks to expand on the concept of amino acid printing, by exploring the possibilities of depositing a sebaceous solution matrix, thereby facilitating the testing of reagents such as Oil-Red-O and Physical Developer. In addition, we present a method that extends capabilities beyond fingermark enhancement reagents for porous substrates, by developing a methodology that enables process validation of the cyanoacrylate fuming technique to be easily facilitated. These simple and effective solutions have the capacity to serve as a crucial process validation check within the laboratory validation workflow.

Evaluation of photometric stereo and elastomeric sensor imaging for the non-destructive 3D analysis of questioned documents - A pilot study.

Photometric Stereo and Elastomeric Sensor Imaging were assessed for measuring the 3-dimensional (3D) morphology of questioned document samples. Photometric stereo is shown to be a powerful non-contact technique for revealing micron level detail of the samples examined. Elastomeric Sensor Imaging is shown to complement photometric stereo by yielding equivalent results. Additionally, this technique allows quantification of the morphological depth information. The techniques were applied to 2 different types of questioned document sample. Firstly, written signatures were examined. Both techniques were able to reveal characteristic features that could be used to infer stroke direction and ink line application sequence. Secondly toner/ink intersections were examined. Both techniques allowed visualisation of 3D features which were used to infer the sequence of application.

Towards the protection of ammunition headstamps during fingermark enhancement processing; a preliminary study.

Forensic recovery from fired ammunition casings remains one of the most challenging tasks during high-profile investigations. Often, the decision must be made between screening for DNA or fingerprints, and, in doing so, the impact these processes will have on the examination of ballistic markings imparted on the ammunition from the firearm itself. Traditionally, fingermark enhancement processes have yielded low success rates in their efforts to identify suspects by enhancing friction ridge detail left on the cartridge casings. Moreover, the enhancement methods utilised may often induce detrimental physical changes to the casing(s), rendering them unsuitable for subsequent ballistics (marking) examination. Recently, new technology has been shown to increase the success rate of fingermark recovery from fired ammunition, and the growing adoption of such innovation means that new challenges are encountered to maximise evidence recovery and streamline forensic workflows. One such example arises from the potential obscuration of the ammunition headstamp area during such treatments. Accordingly, this study outlines the preliminary investigations and developments of a polymer mask substrate that serves to protect the headstamp of fired ammunition casings during relevant fingermark enhancement processes. The technique also has the capacity to be used as a surface protection device to eliminate unwanted chemical deposition across other areas of interest and evidence types.

A preliminary investigation of a two-step, non-invasive process to determine chronological deposition order of fingerprints and printed ink on paper.

While traditional techniques have long allowed forensic investigators to positively identify fingermarks on documents of interest, understanding the chronological sequence of events that led to their deposition is still seen as a 'holy grail' for forensic examinations. By way of example, the question of whether a mark is above or below printed text is crucial. The work herein reveals that a novel application of a recently established fingermark development technique readily allows such differentiation. The process in question allies forensic gelatin lifters with RECOVER, a development system that hinges on the polymerisation of disulfur dinitride. While the latter was specifically developed in its current form for the retrieval of prints from metal surfaces exposed to extreme conditions or washing, its ability to target surface effects allows for visualisation of surface interactions on forensic gelatin lifts. Crucially, in doing so the order in which the lifted material was originally deposited is also revealed. This, therefore, permits clear elucidation of the order of deposition of printed text and fingermarks-and does so both rapidly and in a non-invasive way. This long sought-after capability has the potential to revolutionise forensic document examinations.

Transforming presumptive forensic testing: in situ identification and age estimation of human bodily fluids.

The ability to achieve rapid, in situ identification and age estimation of human bodily fluids can provide valuable information during the investigation of a crime. A novel direct analysis method now permits the rapid in situ identification and age estimation of human bodily fluids for forensic analysis at crime scenes. A thermal desorption surface sampling probe was developed and coupled with a compact mass spectrometer for the direct analysis of volatile organic compound (VOC) profiles of human bodily fluids within two months and in different environmental conditions, without the need for prior sample preparation. The method is not only capable of identifying bodily fluids and discriminating against common interferent species, but also differentiating between bodily fluid stains of different ages over a time period of two months. This demonstrates the potential for rapid in situ identification and age estimation of bodily fluids without the need for contaminative presumptive tests or time-consuming sample preparation.

Fingerprint and inkjet-trace imaging using disulfur dinitride.

Exposure of fingerprints to S(2)N(2) vapour results in the prints being visually imaged by polymeric (SN)(x) on an unprecedented range of media; in addition, the polymer forms in response to the interaction of S(2)N(2) with traces of inkjet inks, for example the minute amounts left by the contact between printed paper and an envelope.

Sequential processing strategies for fingermark visualisation on uncirculated £10 (Bank of England) polymer banknotes.

An investigation was undertaken to assess the relative effectiveness of five fingermark visualisation process sequences on new, uncirculated £10 polymer banknotes from the Bank of England (BoE). Each sequence was challenged with the visualisation of 64 natural fingermarks on eight banknotes, of which half were aged for 2-3days and half were aged for 12-13days (32 donors contributed in each set). The sequences investigated were; After each process was applied, fingermark development was assessed under primary viewing conditions appropriate to the technique (white light or fluorescence). All samples were also assessed under secondary viewing conditions; provided by the optical processes infrared reflection and long-wave ultraviolet reflection, and by gelatin lifting (with scanning). These additional techniques reduced the interfering effect of the complex banknote backgrounds and improved the contrast of enhanced fingermarks. Overall, with all assessment conditions considered, sequence 4 and sequence 3 visualised the most identifiable quality fingermarks (92.2% and 89.1% respectively). The most effective overall process used singularly was found to be black magnetic powder, provided that reflected infrared was used to image the results (82.8%). Further data analysis revealed that sequence 1 was the most effective sequence for fresh marks (aged 2-3days) when only primary viewing conditions within the visible part of the spectrum are considered. The need for further work which considers the sensitivity of the processes, and how they perform on BoE polymer banknotes that have become worn in circulation, is emphasised.

The use of longwave reflected UV imaging for the enhancement of cyanoacrylate developed fingermarks: A simple, safe and effective imaging tool.

Longwave ultraviolet reflection (LWUVR) imaging is reported as a simple, safe and non-invasive technique that significantly aids in the visualisation of cyanoacrylate developed latent fingermarks. The process can precede traditional cyanoacrylate dye staining and often removes the necessity to use these chemical contrast reagents, saving time, cost and eliminating mess. The non-destructive nature of the process and high resolution images that are obtained, builds upon issues that surround shorter-wavelength UV imaging. It has been demonstrated that, for most samples tested (a range of non-porous and semi-porous evidence), LWUVR imaging provided superior or similar results to those obtained using the traditional BY40 dye stain. The lack of penetration depth by LWUV radiation means that only the features of the surface under observation is detected by the camera, meaning that interference that may otherwise arise from fluorescence on the reverse side of the evidence (BY40 or fluorescent inks) under conventional fluorescent dye stain imaging modes, is mitigated. A new sequential processing workflow is proposed that does not impede with the conventional and widely adopted fume>stain>fluorescence sequence, but in fact allows LWUVR imaging to be conducted in a manner that serves to benefit the sequence and, ideally, save time during the examination and treatment of evidence.

Rapid polymerisation of S2N2 within Na-ZSM-5 channels.

Reaction of S(2)N(2) vapour with Na-ZSM-5 results in rapid polymerisation and inclusion of the resulting (SN)(x) within the zeolite channels.

Sunlight-activated near-infrared phosphorescence as a viable means of latent fingermark visualisation.

The chromium-doped zinc gallogermanate, Zn3Ga2Ge2O10:0.5%Cr3+, was prepared and modified for use as a near-infrared (NIR) luminescent fingerprint dusting powder. The optical properties of this material are illustrated and have been shown to offer versatility and promise in terms of overcoming troublesome backgrounds during latent fingermark examination and imaging. The highly fluorescent NIR emission of the powder allows inherently fluorescent and patterned substrate backgrounds to be mitigated, whilst the phosphorescent nature of the material allows all conceivable background interference to be removed, whilst also constituting the first reported use of a NIR phosphor for latent fingermark enhancement and imaging.

Fingermark visualisation on uncirculated £5 (Bank of England) polymer notes: Initial process comparison studies.

Experiments were conducted to investigate the effectiveness of a range of fingermark visualisation processes on brand new, uncirculated, £5 polymer banknotes (and their test note predecessors), as produced by the Bank of England (BoE). In the main study of this paper, a total of 14 individual processes were investigated on BoE £5 polymer banknotes, which included both 'Category A' processes (as recommended in the Home Office Fingermark Visualisation Manual) as well as recently developed processes, including fpNatural® 2 powder (cuprorivaite) from Foster+Freeman and a vacuum metal deposition sequence that evaporates silver followed by zinc. Results from this preliminary investigation indicate that fpNatural® 2, multimetal deposition, Wet Powder™ Black, iron oxide powder suspension and black magnetic powder are the most effective processes on these uncirculated £5 BoE polymer banknotes, when viewed under "primary viewing" conditions (white light or fluorescence). Additional fingermarks were visualised on the polymer banknotes following the subsequent use of reflected infrared imaging and lifting techniques, and with the benefit of these techniques taken into consideration, the aforementioned processes remained amongst the most effective overall. This work provides initial insight into fingermark visualisation strategies for BoE £5 polymer banknotes, and the need for further studies in order to generate mature operational guidance is emphasised.

DNA recovery from latent fingermarks treated with an infrared fluorescent fingerprint powder.

The effect of the infrared fluorescent fingermark visualisation powder, fpNatural 1™, on the recovery of both the quantity and quality of touch DNA from fingerprints deposited on glass slides, was investigated using qPCR and STR typing. Four donors each deposited replicate marks, which were either left untreated (n=5) or treated by dusting with fpNatural 1™ (n=5). Each sample was swabbed using the double swab technique, before being extracted using the EZNA Forensic DNA kit and then DNA quantitated before being subjected to DNA profile analysis. Results showed that there was no significant effect of fpNatural 1™ on either the quantity or quality of recovered DNA. This suggests that fpNatural 1™ may prove a good choice of powder for regular use at crime scenes or in the laboratory. The fpNatural 1™ properties of low density, water immiscibility and low DNA affinity may account for these positive outcomes.

NIR-NIR fluorescence: A new genre of fingermark visualisation techniques.

A preliminary study reveals that finely divided cuprorivaite powder may be used to efficiently develop and subsequently image latent fingermarks across a range of highly patterned, coloured non-porous and semi-porous substrates using near infrared illumination and imaging. Problematic multi-coloured backgrounds provide very little interference under the illumination conditions used, and invoked fluorescence observed, when using this material. This is the first reported example of a NIR-NIR fluorophore for use within latent fingermark visualisation and offers the potential for application at the scene and in the laboratory.

Visualisation of latent fingermarks on polymer banknotes using copper vacuum metal deposition: A preliminary study.

The UK's recent move to polymer banknotes has seen some of the currently used fingermark enhancement techniques for currency potentially become redundant, due to the surface characteristics of the polymer substrates. Possessing a non-porous surface with some semi-porous properties, alternate processes are required for polymer banknotes. This preliminary investigation explored the recovery of fingermarks from polymer notes via vacuum metal deposition using elemental copper. The study successfully demonstrated that fresh latent fingermarks, from an individual donor, could be clearly developed and imaged in the near infrared. By varying the deposition thickness of the copper, the contrast between the fingermark minutiae and the substrate could be readily optimised. Where the deposition thickness was thin enough to be visually indistinguishable, forensic gelatin lifters could be used to lift the fingermarks. These lifts could then be treated with rubeanic acid to produce a visually distinguishable mark. The technique has shown enough promise that it could be effectively utilised on other semi- and non-porous substrates.

Seeing into the infrared: a novel IR fluorescent fingerprint powder.

A preliminary study demonstrates that latent fingermarks across a range of highly patterned, coloured non-porous and semi-porous substrates may be clearly developed and imaged in the near infrared following a simple dusting method using finely divided spirulina platensis powder, a naturally occurring, non-toxic algae, used widely within the food industry. Troublesome printed/multicoloured backgrounds show less interference with the fluorescence observed using this material, unlike conventional luminescent powders which fluoresce in the visible spectrum alongside the background to which they are applied. The material shows promise for use both in the field and in the laboratory.

The recovery of latent text from thermal paper using a simple iodine treatment procedure.

Faded, or actively removed text on thermally printed paper samples may be enhanced and retrieved through the use of a simple iodine fuming procedure. The recovery of printed documentation evidence in this fashion is neither affected by prior fingerprint enhancement techniques (such as ninhydrin or DFO), nor by sample age. This method allows, for the first time, evidence to be obtained from completely faded thermal paper samples (receipts, for example) as well as allowing deliberately removed printed text (a consequence of solvent washing pre-treatment in latent fingerprint enhancement procedures) to be recovered.