RESUMEN
Axonemal dynein motors drive ciliary motility and can consist of up to twenty distinct components with a combined mass of ~2 MDa. In mammals, failure of dyneins to assemble within the axonemal superstructure leads to primary ciliary dyskinesia. Syndromic phenotypes include infertility, rhinitis, severe bronchial conditions, and situs inversus. Nineteen specific cytosolic factors (Dynein Axonemal Assembly Factors; DNAAFs) are necessary for axonemal dynein assembly, although the detailed mechanisms involved remain very unclear. Here, we identify the essential assembly factor DNAAF3 as a structural ortholog of S-adenosylmethionine-dependent methyltransferases. We demonstrate that dynein heavy chains, especially those forming the ciliary outer arms, are methylated on key residues within various nucleotide-binding sites and on microtubule-binding domain helices directly involved in the transition to low binding affinity. These variable modifications, which are generally missing in a Chlamydomonas null mutant for the DNAAF3 ortholog PF22 (DAB1), likely impact on motor mechanochemistry fine-tuning the activities of individual dynein complexes.
Asunto(s)
Dineínas Axonemales , Metiltransferasas , Animales , Citosol , Citoesqueleto , Metilación , MamíferosRESUMEN
Deep eutectic solvents (DES) are mixtures of hydrogen bond donors and acceptors that form strongly hydrogen-bonded room temperature liquids. Changing the H-bonding components and their ratios can alter the physicochemical properties of deep eutectic solvents. Recent studies have shown p-toluenesulfonic acid (pTSA) forms room temperature liquids with choline chloride (ChCl) at different molar ratios: 1 : 1, 1 : 2 and 2 : 1 [Rodriguez Rodriguez et al., ACS Sustain. Chem. Eng., 2019, 7(4), 3940]. They also showed that the composition affects the physical properties of these liquids and their ability to dissolve metal oxides. In this work we evaluate the solubility and self-assembly of cationic surfactants alkyltrimethyl ammonium bromides (CnTAB) in these pTSA/ChCl based liquids. CnTABs are insoluble in 1pTSA : 2ChCl, whereas in 1pTSA : 1ChCl and 2pTSA : 1ChCl they form micelles. We characterise CnTAB (n = 12, 14, 16) micelles using small angle neutron scattering and also look at interaction of water with the micelles. These studies help determine the interaction of DES components with the surfactant and the influence of varying pTSA and water ratios on these interactions. This provides potential for controlled surfactant templating and for tuning rheology modification in such systems.
RESUMEN
Cilia are microtubule-based cellular projections that act as motile, sensory, and secretory organelles. These structures receive information from the environment and transmit downstream signals to the cell body. Cilia also release vesicular ectosomes that bud from the ciliary membrane and carry an array of bioactive enzymes and peptide products. Peptidergic signals represent an ancient mode of intercellular communication, and in metazoans are involved in the maintenance of cellular homeostasis and various other physiological processes and responses. Numerous peptide receptors, subtilisin-like proteases, the peptide-amidating enzyme, and bioactive amidated peptide products have been localized to these organelles. In this review, we detail how cilia serve as specialized signaling organelles and act as a platform for the regulated processing and secretion of peptidergic signals. We especially focus on the processing and trafficking pathways by which a peptide precursor from the green alga Chlamydomonas reinhardtii is converted into an amidated bioactive product-a chemotactic modulator-and released from cilia in ectosomes. Biochemical dissection of this complex ciliary secretory pathway provides a paradigm for understanding cilia-based peptidergic signaling in mammals and other eukaryotes.
Asunto(s)
Chlamydomonas reinhardtii , Cilios , Animales , Cilios/metabolismo , Transducción de Señal , Comunicación Celular , Chlamydomonas reinhardtii/metabolismo , Péptidos/metabolismo , Mamíferos/metabolismoRESUMEN
Cilia are highly complex motile, sensory, and secretory organelles that contain perhaps 1000 or more distinct protein components, many of which are subject to various posttranslational modifications such as phosphorylation, N-terminal acetylation, and proteolytic processing. Another common modification is the addition of one or more methyl groups to the side chains of arginine and lysine residues. These tunable additions delocalize the side-chain charge, decrease hydrogen bond capacity, and increase both bulk and hydrophobicity. Methylation is usually mediated by S-adenosylmethionine (SAM)-dependent methyltransferases and reversed by demethylases. Previous studies have identified several ciliary proteins that are subject to methylation including axonemal dynein heavy chains that are modified by a cytosolic methyltransferase. Here, we have performed an extensive proteomic analysis of multiple independently derived cilia samples to assess the potential for SAM metabolism and the extent of methylation in these organelles. We find that cilia contain all the enzymes needed for generation of the SAM methyl donor and recycling of the S-adenosylhomocysteine and tetrahydrofolate byproducts. In addition, we find that at least 155 distinct ciliary proteins are methylated, in some cases at multiple sites. These data provide a comprehensive resource for studying the consequences of methyl marks on ciliary biology.