RESUMEN
Reports on the incidence of persistent left superior vena cava (PLSVC) in the normal population are limited to studies involving pacemaker implantation candidates and cadavers. The incidence in patients with congenital heart diseases (CHDs) is estimated to be higher than that in the normal population; however, the details are unclear. To investigate the incidence of PLSVC in the normal population and in patients with CHDs, subjects were examined prospectively using echocardiography. Normal subjects consisted of 2841 successive neonates without intra-cardiac or congenital anomalies born in Gifu Prefectural General Medical Center. Additionally, 1920 patients with CHDs were evaluated. The incidence of PLSVC in normal neonates was 0.21% (95% confidence interval 0.042-0.38%). A high incidence (more than 7.0 times the incidence in normal subjects) was observed in all CHD patients. The high incidence group included coarctation of the aorta (CoA) (23.7%) and double outlet right ventricle (DORV) patients (24.6%). The second group consisted of CHD patients with ventricular septal defect (VSD), with an incidence ranging from 5.1 to 6.1%. The low incidence group comprised patients with other CHDs, with an incidence between 1.5 and 3.1%. The incidence of PLSVC in trisomy 21 and atrial septal defect patients was significantly higher than that in normal neonates. The incidence of PLSVC in the normal population and in patients with CHDs was systematically evaluated for the first time. The incidence in CHD patients appeared to be positively influenced by the type of CHD, particularly by DORV, CoA, and VSD.
Asunto(s)
Ecocardiografía/métodos , Cardiopatías Congénitas/complicaciones , Malformaciones Vasculares/epidemiología , Vena Cava Superior/anomalías , Femenino , Cardiopatías Congénitas/diagnóstico por imagen , Humanos , Incidencia , Recién Nacido , Masculino , Estudios Prospectivos , Malformaciones Vasculares/complicaciones , Malformaciones Vasculares/diagnóstico por imagen , Vena Cava Superior/diagnóstico por imagenRESUMEN
Beckwith-Wiedemann syndrome (BWS) is a congenital overgrowth syndrome that is occasionally associated with hyperinsulinemic hypoglycemia (HH) in the neonatal period. Sotos syndrome (SS) and Kabuki syndrome (KS) are other malformation syndromes that may be complicated with HH, however, the detailed clinical characteristics of HH accompanied with these syndromes remain unclear. We herein conducted a nationwide questionnaire survey in Japan. We sent a primary questionnaire concerning the clinical experience for these syndromes to 347 perinatal care institutions. As a result, 222 departments or hospitals returned the questionnaires and the total numbers of BWS, SS, and KS patients were 113, 88, and 51, respectively. We sent a secondary questionnaire to 31 institutions where patients with these syndromes presented with HH during infancy. The secondary questionnaires were returned from the institutions and the numbers of patients were 16 for BWS, 9 for SS, and 3 for KS, respectively. Then, we compared the clinical characteristics of infants suffering from transient HH with and without these dysmorphic syndromes. As a result, BWS, SS, and KS patients showed significantly larger body size, lower Apgar scores, higher insulin levels at HH, and shorter durations of HH than non-dysmorphic infants with transient HH. We propose that a careful observation for the signs of HH, even if not specific to the syndromes, is important for the diagnosis of patients with BWS, SS, and KS in the postnatal period. © 2016 Wiley Periodicals, Inc.
Asunto(s)
Anomalías Múltiples/sangre , Síndrome de Beckwith-Wiedemann/sangre , Cara/anomalías , Enfermedades Hematológicas/sangre , Hiperinsulinismo/sangre , Hipoglucemia/sangre , Síndrome de Sotos/sangre , Enfermedades Vestibulares/sangre , Anomalías Múltiples/diagnóstico , Anomalías Múltiples/epidemiología , Puntaje de Apgar , Síndrome de Beckwith-Wiedemann/diagnóstico , Síndrome de Beckwith-Wiedemann/epidemiología , Femenino , Pruebas Genéticas , Enfermedades Hematológicas/diagnóstico , Enfermedades Hematológicas/epidemiología , Pruebas Hematológicas , Humanos , Recién Nacido , Japón/epidemiología , Masculino , Fenotipo , Vigilancia de la Población , Embarazo , Complicaciones del Embarazo/epidemiología , Síndrome de Sotos/diagnóstico , Síndrome de Sotos/epidemiología , Encuestas y Cuestionarios , Enfermedades Vestibulares/diagnóstico , Enfermedades Vestibulares/epidemiologíaRESUMEN
Vertebrate eggs are surrounded by an egg coat, which is a specific extracellular egg matrix consisting of several glycoproteins with a conserved zona pellucida (ZP) domain. Two mammalian egg coat subunits, ZP2 and ZP3, have been suggested to act as sperm receptors. In bird eggs, however, ZP2 has never been identified in the egg coat of mature oocytes and ovulated eggs. Here we report that chicken ZP2 is expressed in immature small follicles and remains as an egg-coat component locally in the germinal disc region of mature eggs. RT-PCR analysis indicated marked expression of the ZP2 and ZP4 genes in the granulosa cells of immature white follicles, whereas the ZP3 and ZPD genes showed marked expression in the cells of maturing yellow follicles. ZP2 was identified in the egg coat isolated from immature follicles as a heavily N-glycosylated glycoprotein of â¼200 kDa, which was enzymatically converted to a 70-kDa deglycosylated form. Immunoblotting and immunohistological analyses showed that ZP2 was localized around the germinal disc region of mature follicles. ZP2 was accumulated in the egg coat of immature white follicles at the earlier stages of oocyte development and became a minor component in the egg coat of maturing yellow follicles, except for the germinal disc region. Localization of ZP2 in the germinal disc region of mature eggs, where sperm bind to the egg coat at high density, suggests some role for ZP2 in the preferential binding and penetration of sperm in the germinal disc region of bird eggs.
Asunto(s)
Blastodisco/metabolismo , Membrana Celular/metabolismo , Pollos , Proteínas del Huevo/metabolismo , Glicoproteínas de Membrana/metabolismo , Oocitos/metabolismo , Óvulo/metabolismo , Receptores de Superficie Celular/metabolismo , Animales , Pollos/genética , Pollos/metabolismo , Proteínas del Huevo/genética , Cáscara de Huevo/metabolismo , Femenino , Expresión Génica , Glicosilación , Glicoproteínas de Membrana/genética , Oogénesis/fisiología , Receptores de Superficie Celular/genética , Interacciones Espermatozoide-Óvulo/genética , Distribución Tisular , Glicoproteínas de la Zona PelúcidaRESUMEN
Several methods for evaluating left ventricular stroke volume (SV) in neonates using echocardiography have been reported. However, no studies on methodologic comparison of SV with three-dimensional (3D) echocardiography are available. This is the first detailed report on a methodologic comparison of SV in the early neonatal period. The study group included 70 normal neonates (35 boys and 35 girls). An iE33 echocardiograph and Q-LAB supplied by Philips Electronics were used to examine and calculate volumes. Comparisons of SV were performed using Teichholz (T), the velocity time integral (VTI), Pombo (P), modified Simpson (MS), and 3D methods with normal neonates who had no persistent ductus arteriosus less than 7 days after birth. The mean SVs were 33.7 mL/m(2) (T), 30.6 mL/m(2) (VTI), 22.0 mL/m(2) (P), 17.5 mL/m(2) (3D), and 14.9 mL/m(2) (MS) using Haycock's formula of body surface area. The stroke volumes differed significantly depending on the different methods. The correlation coefficient was highest between the MS and 3D methods. The SVs of the T and VTI methods were significantly greater than those previously reported, and it seemed inappropriate to evaluate volumes in neonates. The 3D and MS methods were appropriate for measuring SV in neonates during the early neonatal period.
Asunto(s)
Ecocardiografía/métodos , Recién Nacido/fisiología , Volumen Sistólico/fisiología , Función Ventricular Izquierda/fisiología , Ecocardiografía Tridimensional/métodos , Femenino , Humanos , Masculino , Estadística como AsuntoRESUMEN
Avidin from egg white was migrated toward a cathode of nondenaturing electrophoresis and then immobilized on a polyvinylidene difluoride membrane. Adrenocorticotropic hormone (ACTH) was specifically captured after the biotinylated anti-ACTH antibody was bound to the membrane-immobilized avidin, and the captured ACTH was digested by the biotinylated trypsin on the membrane after extraction. The digested polypeptides from the ACTH were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). These results indicate that target substances can be specifically trapped and digested on membrane-immobilized avidin.
Asunto(s)
Complejo Antígeno-Anticuerpo/química , Antígenos/análisis , Avidina/química , Proteínas Inmovilizadas/química , Membranas/química , Proteolisis , Hormona Adrenocorticotrópica/química , Anticuerpos/química , Antígenos/química , Avidina/análisis , Clara de Huevo/química , Electrodos , Activación Enzimática , Humanos , Técnicas de Inmunoadsorción , Péptido Hidrolasas/química , Polivinilos/química , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Coloración y Etiquetado , Tripsina/químicaRESUMEN
Adrenocorticotropic hormone (ACTH) and transferrin were trapped by biotinylated anti-ACTH antibody and anti-transferrin antibody, respectively, bound to membrane-immobilized avidin. Polypeptides with the sequences SYSMEHFR, SYSMEHFRWGKPVGK and SYSMEHFRWGKPVGKK were bound to the biotinylated anti-ACTH antibody on the membrane-immobilized avidin after the trapped ACTH was digested with trypsin on the membrane and non-binding polypeptides were washed from the membrane. Further, the polypeptides with the sequence SYSMEHFRWGKPVGK and SYSMEHFRWGKPVGKK were trapped by anti-ACTH antibody bound to membrane-immobilized protein A. The antibody recognized the WGKPVGK region of the antigen, ACTH. Polypeptide with the sequence SMGGKEDLIWELLNQAQEHFGKDK was bound to the biotinylated anti-transferrin antibody on the membrane-immobilized avidin after the trapped transferrin was digested with trypsin on the membrane and non-binding polypeptides were washed from the membrane. Further, the polypeptide with the sequence SMGGKEDLIWELLNQAQEHFGKDK was trapped by anti-transferrin antibody bound to membrane-immobilized protein A. The antibody recognized the SMGGKEDLIWELLNQAQEHFGKDK region of the antigen, transferrin. These results thus indicate that the combined methods of membrane-immobilized avidin and protein A can be applied to examine the epitopes of antigens.
Asunto(s)
Avidina/metabolismo , Epítopos/análisis , Proteínas Inmovilizadas/metabolismo , Proteína Estafilocócica A/metabolismo , Hormona Adrenocorticotrópica/análisis , Hormona Adrenocorticotrópica/química , Hormona Adrenocorticotrópica/metabolismo , Secuencia de Aminoácidos , Animales , Anticuerpos/metabolismo , Avidina/química , Epítopos/química , Epítopos/metabolismo , Humanos , Proteínas Inmovilizadas/química , Membranas Artificiales , Datos de Secuencia Molecular , Fragmentos de Péptidos/análisis , Fragmentos de Péptidos/química , Fragmentos de Péptidos/metabolismo , Polivinilos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Proteína Estafilocócica A/química , Porcinos , Transferrina/análisis , Transferrina/química , Transferrina/metabolismo , Tripsina/metabolismoRESUMEN
BACKGROUND: Indomethacin is used to treat the hemodynamically significant patent ductus arteriosus in premature infants. Some infants show ductus arteriosus reopening after effective constriction by the drug. The purpose of this study was to examine the clinical characteristics of such infants. METHODS: We studied 57 very-low-birthweight infants with effective constriction of patent ductus arteriosus by the initial course of indomethacin. They were classified into the reopened group if they developed hemodynamically significant patent ductus arteriosus again or into the closed group if they showed complete closure. Clinical characteristics were compared between the two groups. RESULTS: Ductus arteriosus reopening was shown in 15 (26%) of the 57 infants. These 15 infants had successful clinical ductal closure after a subsequent course of indomethacin or oral mefenamic acid treatment or surgical ligation without any severe complications. Infants in the reopened group showed significantly higher rates of developing chronic lung disease at 36 weeks of gestation than those in the closed group (53% vs 18%; P= 0.009). Furthermore, multivariate logistic regression analysis revealed ductus arteriosus reopening was the only independent risk factor for developing chronic lung disease at 36 postconceptional weeks in this population (adjusted odds ratio, 6.1; 95% confidence interval, 1.4-31.2; P= 0.02). CONCLUSIONS: Incomplete closure of the ductus arteriosus is associated with recurrence of a clinically significant patent ductus arteriosus and reopening of the ductus after initial closure with indomethacin is associated with chronic lung disease.
Asunto(s)
Conducto Arterioso Permeable/tratamiento farmacológico , Conducto Arterial/efectos de los fármacos , Hemodinámica/efectos de los fármacos , Indometacina/administración & dosificación , Recién Nacido de muy Bajo Peso , Administración Oral , Antiinflamatorios no Esteroideos/administración & dosificación , Relación Dosis-Respuesta a Droga , Conducto Arterial/fisiopatología , Conducto Arterioso Permeable/epidemiología , Conducto Arterioso Permeable/fisiopatología , Femenino , Estudios de Seguimiento , Humanos , Recién Nacido , Masculino , Recurrencia , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
Idiopathic perinatal cerebral infarction is recognized to be more common in the preterm infant than previously realized. However, the pathogenic mechanisms and the onset time remain unclear. We encountered an extremely low birth weight female infant with severe intrauterine growth retardation and brain infarction localized on the left inferior temporal gyrus. The onset of the infarction and the precise sequential changes were evaluated with blood data, cranial ultrasound imaging, computed tomography, magnetic resonance imaging and magnetic resonance angiography. This is the first published fetal case with brain infarction specifically localized on the left inferior temporal gyrus. Careful observation with serial brain imaging is indispensable for high-risk infarction groups, such as preterm infants with severe intrauterine growth retardation, to detect neurological abnormality earlier and precisely.
Asunto(s)
Infarto Encefálico/patología , Enfermedades del Prematuro/patología , Lóbulo Temporal/patología , Adulto , Infarto Encefálico/diagnóstico por imagen , Cesárea , Femenino , Retardo del Crecimiento Fetal/patología , Humanos , Recién Nacido , Recien Nacido Prematuro , Enfermedades del Prematuro/diagnóstico por imagen , Embarazo , Lóbulo Temporal/diagnóstico por imagen , UltrasonografíaRESUMEN
We report the case of a boy with a de novo partial monosomy 16p13-pter and partial trisomy 16q22-qter detected by fluorescence in situ hybridization using subtelomeric probes for 16p and 16q. The boy had facial characteristics, skeletal features, congenital heart defects, an imperforate anus, urogenital malformations, pre/postnatal growth retardation, and psychomotor retardation, most of which have been reported both in partial monosomy 16p and partial trisomy 16q. In addition, he suffered from upper airway stenosis due to possible laryngeal stenosis with subglottic webs. The upper airway stenosis could be a rare complication of partial monosomy 16p or partial trisomy 16q, or a nonspecific malformation resulting from chromosomal abnormalities.
Asunto(s)
Obstrucción de las Vías Aéreas/genética , Cromosomas Humanos Par 16/genética , Monosomía , Estenosis Traqueal/genética , Trisomía , Adulto , Obstrucción de las Vías Aéreas/diagnóstico , Femenino , Humanos , Hibridación Fluorescente in Situ , Recién Nacido , Cariotipificación , Masculino , Estenosis Traqueal/diagnósticoRESUMEN
OBJECTIVE: The mean closure time of the ductus arteriosus (DA) in full-term neonates is presumed to be 1-2â days after birth; however, whether this rate is accurate throughout the neonatal period is still unclear. In addition, the clinical determinants that influence DA closure remain unknown. METHODS: Echocardiography was performed 1826 times (897 in boys, 929 in girls) in 1442 participants (732 boys, 710 girls). An iE33 colour Doppler echocardiograph supplied by Philips Electronics was employed to examine DA flow. Data regarding sex, birth date, examination date, method of delivery, mother's age, past deliveries, neonatal body weight and body height were also collected. The Statistical Analysis System makes statistical clarification of these queries possible. We examined the persistence of DA in full-term neonates and appropriate for gestational age (AGA) neonates in the early neonatal period using colour Doppler echocardiography, and a subsequent analysis with SAS. RESULTS: After performing multivariable analyses, the median DA persistency times were 27.42 and 45.10â h after birth in boys and girls, respectively. A statistically significant sex difference was observed (p<0.0001). Additionally, significant time differences were observed between vaginal and scheduled caesarean deliveries, at 26.97 and 28.93â h, respectively (p=0.0245). No significant differences were observed in the other variables. CONCLUSIONS: Spontaneous DA closure time curves were clarified for the first time throughout the early neonatal period in full-term and AGA neonates. It was revealed that both sex and delivery method play important roles in time to DA closure.
RESUMEN
Fertilization begins with interaction between the sperm and the egg. The surface of the vertebrate oocyte is covered with the egg envelope, which is composed of ZP (zona pellucida) glycoproteins. We have identified two glycoproteins, ZP1/gp97 and ZPC/gp42, as the major components of the chicken egg envelope. In the present study, another 42 kDa protein, designated ZPD, has been found as a new major component of the chicken egg envelope. ZPD was specifically released from the egg envelope by ultrasonication treatment without urea. ZPD cDNA was cloned using a chicken granulosa cell cDNA pool. The deduced amino acid sequence showed that preproprotein of ZPD is composed of 418 amino acid residues with four potential N-glycosylation sites and includes a ZP domain, common in vertebrate ZP glycoproteins, and a transmembrane domain. ZPD belongs phylogenetically to a distinct group from known ZP glycoprotein subfamilies, ZPA, ZPB, and ZPC. In two-dimensional gel electrophoresis ZPD proteins were identified to be several isoforms with different pI values between 5 and 7. ZP1, ZPC and the newly identified ZPD were confirmed to be the major components of chicken egg envelope by MS of proteolytic digests of whole egg envelope. The in vitro incubation of chicken sperm with calcium ionophore A23187 induced sperm activation, resulting in the fragmentation and release of a 41 kDa PNA (peanut agglutinin)-positive glycoprotein and the decrease or loss of sperm PNA-stainability. The incubation with ZPD and dimeric ZP1, but not ZPC and monomeric ZP1, also induced the decrease or loss of sperm PNA-stainability, suggesting the in vitro sperm activation by these ZP components. Collectively, ZPD might bind loosely to egg envelope matrix and play a key role in the sperm activation on avian sperm-egg interaction.
Asunto(s)
Pollos , Proteínas del Huevo/genética , Glicoproteínas de Membrana/genética , Receptores de Superficie Celular/genética , Secuencia de Aminoácidos/genética , Animales , Secuencia de Bases/genética , ADN Complementario/genética , Dimerización , Proteínas del Huevo/química , Proteínas del Huevo/fisiología , Femenino , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/fisiología , Datos de Secuencia Molecular , Folículo Ovárico/química , Folículo Ovárico/metabolismo , Receptores de Superficie Celular/química , Receptores de Superficie Celular/fisiología , Interacciones Espermatozoide-Óvulo/fisiología , Glicoproteínas de la Zona PelúcidaRESUMEN
Initiator codon mutations are relatively uncommon and less well characterized compared to other types of mutations. We identified a novel initiator codon mutation (c.2T>C) heterozygously in a Japanese patient (Patient GK30) with mitochondrial acetoacetyl-CoA thiolase (T2) gene deficiency (ACAT1 deficiency); c.149delC was on the other allele. We examined translation efficiencies of nine mutant T2 cDNAs harboring one-base substitutions at the initiator methionine codon using in vivo transient expression analysis. We found that all the mutants produced wild-type T2 polypeptide, to various degrees (wild type (100%) > c.1A>C (66%) > c.2T>C, c.3G>C, c.3G>T (22%) > c3G>A, c.1A>G (11%) > c.2T>A, c.2T>G, c.1A>T (7.4%)). T2 mRNA expression levels in Patient GK08 (a homozygote of c.2T>A) and Patient GK30 fibroblasts, respectively, were almost the same as in control fibroblasts, when examined using semiquantitative PCR. This means that initiator codon mutations did not affect T2 mRNA levels. We propose that all one-base substitutions at the initiator methionine codon in the T2 gene could be mutations, which retain some residual T2 activity.
Asunto(s)
Acetil-CoA C-Acetiltransferasa/genética , Codón Iniciador/genética , Mitocondrias/enzimología , Mitocondrias/genética , Péptidos/genética , Mutación Puntual/genética , Acetil-CoA C-Acetiltransferasa/biosíntesis , Acetil-CoA C-Acetiltransferasa/deficiencia , Línea Celular , Análisis Mutacional de ADN , ADN Complementario/genética , Femenino , Heterocigoto , Humanos , Lactante , Japón , Masculino , Datos de Secuencia Molecular , Biosíntesis de Proteínas , ARN Mensajero/genética , ARN Mensajero/metabolismoRESUMEN
BACKGROUND: The indications for ductus arteriosus ligation in very-low-birth-weight infants (VLBWIs) with persistent ductus arteriosus (PDA) are unclear. Increased left ventricular end-diastolic dimension (LVDd) is commonly found in patients with PDA. Here, the enlargement of LVDd in term and preterm neonates without congenital heart disease was estimated by two-dimensional echocardiography. METHODS: The value of the measured LVDd was divided by the normal LVDd as an index (LVDd ratio) to compare 30 patients who underwent PDA ligation with 30 patients treated with indomethacin and 30 patients who did not undergo radical therapy. RESULTS: An LVDd ratio between 122% and 197% (mean, 142%) was considered to be an indication for the ligation procedure. The proportion of patients exceeding 130% in the LVDd ratio was 87% (26/30) in those patients who underwent ligation. Catecholamines and/or vasodilators were required in 83% patients for the treatment of low ejection fraction or hypertension after operations, suggesting that patients had been in preload and/or afterload remodeling failure during the operation. The percentage of patients with less than 115% in the LVDd ratio was 90% in the non-radical-therapy patients. The LVDd ratios of 130% and 115% were regarded as cut-off values for surgical ligation and indomethacin treatment. CONCLUSION: The LVDd ratio is a useful measure to determine the treatment of VLBWIs with PDA.
Asunto(s)
Conducto Arterioso Permeable/diagnóstico por imagen , Ventrículos Cardíacos/diagnóstico por imagen , Enfermedades del Prematuro/diagnóstico por imagen , Fármacos Cardiovasculares/uso terapéutico , Conducto Arterioso Permeable/tratamiento farmacológico , Ecocardiografía , Femenino , Humanos , Indometacina/uso terapéutico , Recién Nacido , Recien Nacido Prematuro , Enfermedades del Prematuro/cirugía , Recién Nacido de muy Bajo Peso , Ligadura , MasculinoRESUMEN
Spondylocostal dysostosis (SCD) is a very rare syndrome characterized by vertebral malformation and rib deformity. Some of the patients with SCD have other birth defects in the central nervous system, the genitourinary tract, diaphragm or heart and so forth. There have been reported SCD with complex congenital heart disease, such as pulmonary atresia, double outlet right ventricle, and d-transposition of great arteries. However, there have been no reported SCD patients with confirmed tetralogy of Fallot (TOF). Here, a patient with SCD having a very rare combination of rib defects on the right side and left-sided scoliosis, tetralogy of Fallot, and diaphragmatic spleen herniation, which had not been reported before, was described.
Asunto(s)
Anomalías Múltiples/diagnóstico por imagen , Cardiopatías Congénitas/diagnóstico por imagen , Hernia Diafragmática/diagnóstico por imagen , Tetralogía de Fallot/diagnóstico por imagen , Diafragma/anomalías , Diafragma/diagnóstico por imagen , Resultado Fatal , Humanos , Lactante , Recién Nacido , Masculino , Radiografía , Bazo/anomalías , Bazo/diagnóstico por imagenRESUMEN
Cerebro-costo-mandibular syndrome (CCMS) is a very rare syndrome characterized by micrognathia and posterior rib gap, with a poor prognosis. To date, only 75 cases have been reported worldwide. The overall survival rate for patients with this disorder has not been reported, and a classification of the patients on the basis of the prognosis is not yet available. The present study analyzed the figures and prognoses of past patients and documented a new case of CCMS. Formerly published case reports and personal communications were used to reveal the prognosis and classification of CCMS. The occurrence ratios of rib gap defects and of missing ribs were examined. Patients were divided into the following three groups according to their life span: lethal type, where the patients died before 1 month; severe type, where the patients lived for 1-12 months; and mild type, where they survived for more than 1 year. A comparison was made of the number of rib gaps, missing ribs, and the rib gap ratio (defined as the number of rib gaps divided by the number of all existing ribs) among these three groups. A significant difference in the number of rib defects between the lethal type and other types was noted. Short life span of severe type patients, compared to mild type, was attributed to their subjection to severe respiratory infection. CCMS can be classified into three categories--lethal, severe, and mild--according to the severity of the symptoms and prognosis.
Asunto(s)
Anomalías Múltiples/clasificación , Anomalías Craneofaciales/clasificación , Costillas/anomalías , Anomalías Múltiples/diagnóstico , Preescolar , Femenino , Humanos , Lactante , Recién Nacido , Micrognatismo/clasificación , Micrognatismo/diagnóstico , Pronóstico , SíndromeRESUMEN
Neonatal disseminated herpes simplex virus (HSV) infection with acute liver failure (ALF) and neonatal hemophagocytic lymphohistiocytosis (HLH) are severe diseases. We recently experienced a male infant with HLH and ALF induced by HSV type 1 (HSV-1). The infant, born at 39 weeks of gestation by normal delivery, developed a fever on day 4. On day 9, laboratory investigations showed progressive liver dysfunction and coagulopathy, and the serum ferritin was excessively elevated. Furthermore, the blood levels of interleukin (IL)-6, IL-10, and interferon-gamma were also elevated. HSV-1 DNA was detected in the serum and cerebrospinal fluid by the real-time PCR method. A diagnosis of HLH was established based upon the following criteria: fever, splenomegaly, cytopenia (two cell lines), serum ferritin (> 500 mug/l) and hypofibrinogenemia (< 150 mg/dl). High-dose acyclovir therapy, steroid pulse therapy using methylprednisolone, high-dose gamma globulin therapy and a blood transfusion were given. The patient recovered without neurological deficit. Neonatal disseminated HSV infections may be complicated by the development of HLH and hypercyokinemia. If HLH is suspected, not only high-dose acyclovir therapy but also anti-cytokine therapy should be considered.
Asunto(s)
Herpes Simple/complicaciones , Herpes Simple/terapia , Herpesvirus Humano 1 , Fallo Hepático Agudo/complicaciones , Fallo Hepático Agudo/terapia , Linfohistiocitosis Hemofagocítica/complicaciones , Linfohistiocitosis Hemofagocítica/terapia , Aciclovir/uso terapéutico , Antiinflamatorios/uso terapéutico , Antivirales/uso terapéutico , Transfusión Sanguínea , Coma/complicaciones , Citocinas/antagonistas & inhibidores , Citocinas/sangre , ADN Viral/sangre , ADN Viral/genética , Escala de Coma de Glasgow , Humanos , Recién Nacido , Pruebas de Función Hepática , Masculino , Metilprednisolona/uso terapéutico , gammaglobulinas/uso terapéuticoRESUMEN
In the phylogenetic tree, selenoproteins and the corresponding translation machinery are found in Archaea, Eubacteria, and animals, but not in fungi and higher plants. As very little is known about Protozoa, we searched for the presence of selenoproteins in the primitive dinoflagellate Oxyrrhis marina, belonging to the Protoctista kingdom. Four selenoproteins could be obtained from O. marina cells cultured in the presence of 75Se. Using O. marina or bovine liver cytosolic extracts, we could serylate and selenylate in vitro total O. marina tRNAs. Moreover, the existence of a tRNA(Sec) could be deduced from in vivo experiments. Lastly, an anti-serum against the specialized mammalian translation elongation factor mSelB reacted with a protein of 48-kDa molecular mass. Altogether, our data showed that O. marina contains selenoproteins and suggests that the corresponding translation machinery is related to that found in animals.
Asunto(s)
Dinoflagelados/genética , Dinoflagelados/metabolismo , Proteínas/genética , Proteínas/metabolismo , Animales , Bovinos , Técnicas In Vitro , Biosíntesis de Proteínas , ARN Protozoario/genética , ARN Protozoario/metabolismo , Aminoacil-ARN de Transferencia/genética , Aminoacil-ARN de Transferencia/metabolismo , Selenocisteína/metabolismo , SelenoproteínasRESUMEN
Mitochondrial acetoacetyl-CoA thiolase (T2) deficiency is an inborn error of ketone body and isoleucine metabolism. We identified and characterized 6 mutations, DelE85, K124R, A127V, Q145E, G152A, and E345V in 5 Spanish T2-deficient patients. Transient expression of mutant cDNAs was done at 37 and at 30 degrees C. Expression of the Q145E mutant cDNA resulted in about 12.5% normal amount at 37 degrees C and it retained 15% residual T2, indicating that specific activity of Q145E mutant protein was almost normal. This mutation reduced the heat stability of T2 activity. Although no significant residual activity was detected in either the G152A and A127V substitution, mutant proteins were detected, at 12.5% the normal amount at 37 degrees C and one-half normal at 30 degrees C for A127V, and 25 % only at 30 degrees C for G152A. Mutant proteins with Q145E, G152A, or A127V accumulated at 30 degrees C expression were stable for 48 h at 37 degrees C after cycloheximide treatment. Expression of DelE85, K124R, and E345V cDNAs gave neither residual T2 protein nor T2 activity. We constructed an improved tertiary structural model of T2 based on the X-ray crystal structure of acetoacetyl-CoA thiolase of Zoogloea ramigera. On the basis of this model, K124, A127, and G152 are located near the active site, mutations of which might affect catalytic function whereas Q145E, De185E, and E345V are distant from the active site with mutants being expected to destabilize the tertiary structure, especially during protein folding and dimerization.
Asunto(s)
Acetil-CoA C-Aciltransferasa/deficiencia , Errores Innatos del Metabolismo/genética , Mitocondrias/enzimología , Acetil-CoA C-Aciltransferasa/química , Acetil-CoA C-Aciltransferasa/genética , Secuencia de Aminoácidos , Sustitución de Aminoácidos , ADN/química , ADN/genética , Análisis Mutacional de ADN , ADN Complementario/química , ADN Complementario/genética , Estabilidad de Enzimas , Femenino , Humanos , Immunoblotting , Lactante , Errores Innatos del Metabolismo/enzimología , Modelos Moleculares , Datos de Secuencia Molecular , Mutación , Estructura Terciaria de Proteína , Homología de Secuencia de AminoácidoRESUMEN
Propionic acidemia (PA) is an inborn error of organic acid metabolism caused by a deficiency of propionyl-CoA carboxylase. This enzyme is composed of two non-identical subunits, alpha and beta, which are encoded by the PCCA and PCCB genes, respectively. An enzyme deficiency can result from mutations in either PCCA or PCCB. To elucidate the mutation spectrum in Japanese patients, we have performed a mutation analysis of 30 patients with PA, which included nine previously reported patients. The study revealed that 15 patients were alpha-subunit deficient and 15 patients were beta-subunit deficient. Seven novel mutations were found (IVS18-6C >G, 1746G >A, C398R, G197E and IVS18+1G >A in the PCCA; A153P and IVS9+1G >T in the PCCB). Among these Japanese patients with alpha-subunit deficiencies, 923-924insT, IVS18-6C >G, and R399Q mutations were frequent and the total allelic frequency of these three mutations combined was 56% (17/30). This is in sharp contrast to the mutation spectrum found in Caucasian patients, where no prevalent mutations have been identified. Among the beta-subunit deficiencies, there were three frequent mutations; R410W, T428I, and A153P, whose allelic frequencies were 30, 26.7, and 13.3%, respectively. In conclusion, a limited number of mutations are predominant in both PCCA and PCCB genes among Japanese patients with propionic acidemia.