RESUMEN
The article presents the results of investigation of antitumor properties of platinum-arabinogalactan complex. We showed the ability of the complex to inhibit the growth of Ehrlich ascites tumor cells. It is found that the distribution of the platinum-arabinogalactan complex is not specific only for tumor cells in mice. The complex was found in all tissues and organs examined (ascites cells, embryonic cells, kidney, and liver). The mechanism of action of the arabinogalactan-platinum complex may be similar to cisplatin as the complex is able to accumulate in tumor cells.
Asunto(s)
Antineoplásicos/farmacología , Carcinoma de Ehrlich/tratamiento farmacológico , Galactanos/farmacología , Compuestos Organoplatinos/farmacología , Animales , Antineoplásicos/síntesis química , Antineoplásicos/farmacocinética , Ascitis/tratamiento farmacológico , Ascitis/metabolismo , Carcinoma de Ehrlich/metabolismo , Cisplatino/farmacología , Evaluación Preclínica de Medicamentos , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Galactanos/síntesis química , Galactanos/farmacocinética , Riñón/efectos de los fármacos , Riñón/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Linfocitos/efectos de los fármacos , Linfocitos/metabolismo , Masculino , Ratones Endogámicos ICR , Microscopía Fluorescente , Trasplante de Neoplasias , Compuestos Organoplatinos/síntesis química , Compuestos Organoplatinos/farmacocinéticaRESUMEN
A molecular biosensor based on DNA aptamers (aptasensor) for the diagnosis of lung cancer in blood plasma samples was designed. To create the aptasensor, the aptamer 17_80, obtained in the study of postoperative material, was used. The affinity and binding selectivity of the aptamer 17_80 to the lung tumor tissue was confirmed on histological sections of postmortem samples of lung tissue. Using affinity enrichment and mass spectrometry, a possible target molecule of the aptamer 17_80, vimentin, was found.
Asunto(s)
Aptámeros de Nucleótidos/química , Biomarcadores de Tumor/sangre , Técnicas Biosensibles/métodos , Carcinoma de Células Escamosas/sangre , Detección Precoz del Cáncer/métodos , Neoplasias Pulmonares/sangre , Proteínas de Neoplasias/sangre , Adulto , Anciano , Aptámeros de Nucleótidos/farmacología , Biomarcadores de Tumor/metabolismo , Humanos , Persona de Mediana Edad , Proteínas de Neoplasias/metabolismo , Proteínas Oncogénicas , Unión ProteicaRESUMEN
Here we describe a method of forming large arrays (up to 10(9) pieces) of free magnetic Ni-nanodisks 50 nm thick coated on both sides with layers of 5 nm thick Au. The antitumor effect of the magnetic nickel gold-coated nanodisks and DNA aptamer conjugates was evaluated in vivo and in vitro. Under the influence of rotating magnetic field, the studied nanodisks can cause the death of Ehrlich ascites carcinoma cells.
Asunto(s)
Antineoplásicos/administración & dosificación , Aptámeros de Nucleótidos/administración & dosificación , Carcinoma de Ehrlich/tratamiento farmacológico , Nanopartículas del Metal/química , Animales , Antineoplásicos/uso terapéutico , Aptámeros de Nucleótidos/uso terapéutico , Línea Celular Tumoral , Oro/química , Campos Magnéticos , Masculino , Nanopartículas del Metal/efectos adversos , Ratones , Ratones Endogámicos ICR , Níquel/químicaRESUMEN
The effects of cisplatin on [Ca2+]in, pHin, NAD(P)H level, and lymphocyte membrane microviscosity were studied in mice with Ehrlich ascitic carcinoma. The level of free [Ca2+]in in lymphocytes from mice with Ehrlich ascitic carcinoma was 4-fold reduced compared to that in intact animals on day 13 of tumor development, while [H+]in level was elevated. Cisplatin caused no changes in the level of free Ca2+, but reduced cytosol acidification. Lymphocyte membrane fluidity in mice with tumors was increased in the lipid bilayer and in the protein-lipid contact zone and did not depend on cisplatin treatment. The level of NAD(P)H was low in mice with tumors, but increased sharply after cisplatin treatment. It seems that functional activity of lymphocytes decreased at the stage of well-developed tumor, which promoted inhibition of the lymphocyte defense properties. Cisplatin did not modify the structure and functions of lymphocytes and presumably even improved their energy status.