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1.
Cell ; 166(2): 506-516, 2016 Jul 14.
Artículo en Inglés | MEDLINE | ID: mdl-27419874

RESUMEN

Because of cellular heterogeneity, the analysis of endogenous molecules from single cells is of significant interest and has major implications. While micromanipulation or cell sorting followed by cell lysis is already used for subsequent molecular examinations, approaches to directly extract the content of living cells remain a challenging but promising alternative to achieving non-destructive sampling and cell-context preservation. Here, we demonstrate the quantitative extraction from single cells with spatiotemporal control using fluidic force microscopy. We further present a comprehensive analysis of the soluble molecules withdrawn from the cytoplasm or the nucleus, including the detection of enzyme activities and transcript abundances. This approach has uncovered the ability of cells to withstand extraction of up to several picoliters and opens opportunities to study cellular dynamics and cell-cell communication under physiological conditions at the single-cell level.


Asunto(s)
Microscopía de Fuerza Atómica/métodos , Nanotecnología/métodos , Análisis de la Célula Individual/métodos , Extractos Celulares/análisis , Células HeLa , Humanos , Microscopía Electrónica de Transmisión , Transcriptoma
2.
EMBO J ; 38(10)2019 05 15.
Artículo en Inglés | MEDLINE | ID: mdl-30877094

RESUMEN

Bacteria have evolved macromolecular machineries that secrete effectors and toxins to survive and thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine that is related to Myoviridae phages. It is composed of a phage tail-like structure inserted in the bacterial cell envelope by a membrane complex (MC) comprising the TssJ, TssL and TssM proteins. We previously reported the low-resolution negative-stain electron microscopy structure of the enteroaggregative Escherichia coli MC and proposed a rotational 5-fold symmetry with a TssJ:TssL:TssM stoichiometry of 2:2:2. Here, cryo-electron tomography analyses of the T6SS MC confirm the 5-fold symmetry in situ and identify the regions of the structure that insert into the bacterial membranes. A high-resolution model obtained by single-particle cryo-electron microscopy highlights new features: five additional copies of TssJ, yielding a TssJ:TssL:TssM stoichiometry of 3:2:2, an 11-residue loop in TssM, protruding inside the lumen of the MC and constituting a functionally important periplasmic gate, and hinge regions. Based on these data, we propose an updated model on MC structure and dynamics during T6SS assembly and function.


Asunto(s)
Sistemas de Secreción Tipo VI/química , Sistemas de Secreción Tipo VI/metabolismo , Sistemas de Secreción Bacterianos/química , Sistemas de Secreción Bacterianos/metabolismo , Membrana Celular/química , Membrana Celular/metabolismo , Microscopía por Crioelectrón , Escherichia coli/metabolismo , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Proteínas de la Membrana/química , Proteínas de la Membrana/metabolismo , Modelos Moleculares , Unión Proteica , Estructura Cuaternaria de Proteína
3.
Chem Soc Rev ; 43(2): 506-17, 2014 Jan 21.
Artículo en Inglés | MEDLINE | ID: mdl-24161958

RESUMEN

MicroRNAs are being considered as a novel type of bio-markers and potential therapeutic targets for various diseases. Diverse chemical tools are being developed for the detection or regulation of microRNAs with bio-medical implications. Chemical probes have been developed for use in combination with in situ signal amplification strategies to realize sensitive detection of microRNAs of low abundance. Regulation of microRNAs aberrantly expressed in tumours represents a new approach to cancer chemotherapy. Synthetic oligonucleotides including antisense oligonucleotides and microRNA mimics have been successfully delivered into cells or tissues to inhibit or enhance the function of specific endogenous microRNAs. Small-molecule modifiers of microRNAs that modify the expression or function of endogenous microRNAs are emerging not only as useful probes to explore microRNA-involved regulatory networks, but also as potential therapeutic reagents. In this tutorial review, we discuss the strategies developed by chemists in recent years for microRNA detection and regulation, with a focus on the potential of these chemical tools in microRNA-related biomedical applications.


Asunto(s)
Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , MicroARNs/genética , Sondas Moleculares , Neoplasias/diagnóstico , Oligonucleótidos Antisentido/genética , Animales , Colorantes Fluorescentes/química , Grafito/química , Humanos , MicroARNs/agonistas , MicroARNs/antagonistas & inhibidores , Técnicas de Sonda Molecular , Sondas Moleculares/química , Nanopartículas/química , Nanopartículas/uso terapéutico , Neoplasias/genética , Neoplasias/terapia , Oligonucleótidos Antisentido/síntesis química , Oligonucleótidos Antisentido/uso terapéutico , Óxidos
4.
Front Microbiol ; 11: 1373, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32670237

RESUMEN

The cell biology of Chloroflexota is poorly studied. We applied cryo-focused ion beam milling and cryo-electron tomography to study the ultrastructural organization of thermophilic Roseiflexus castenholzii and Chloroflexus aggregans, and mesophilic "Ca. Viridilinea mediisalina." These species represent the three main lineages within a group of multicellular filamentous anoxygenic phototrophic Chloroflexota bacteria belonging to the Chloroflexales order. We found surprising structural complexity in the Chloroflexales. As with filamentous cyanobacteria, cells of C. aggregans and "Ca. Viridilinea mediisalina" share the outer membrane-like layers of their intricate multilayer cell envelope. Additionally, cells of R. castenholzii and "Ca. Viridilinea mediisalina" are connected by septal channels that resemble cyanobacterial septal junctions. All three strains possess long pili anchored close to cell-to-cell junctions, a morphological feature comparable to that observed in cyanobacteria. The cytoplasm of the Chloroflexales bacteria is crowded with intracellular organelles such as different types of storage granules, membrane vesicles, chlorosomes, gas vesicles, chemoreceptor-like arrays, and cytoplasmic filaments. We observed a higher level of complexity in the mesophilic strain compared to the thermophilic strains with regards to the composition of intracellular bodies and the organization of the cell envelope. The ultrastructural details that we describe in these Chloroflexales bacteria will motivate further cell biological studies, given that the function and evolution of the many discovered morphological traits remain enigmatic in this diverse and widespread bacterial group.

5.
Nat Commun ; 11(1): 2041, 2020 04 27.
Artículo en Inglés | MEDLINE | ID: mdl-32341341

RESUMEN

How complex, multi-component macromolecular machines evolved remains poorly understood. Here we reveal the evolutionary origins of the chemosensory machinery that controls flagellar motility in Escherichia coli. We first identify ancestral forms still present in Vibrio cholerae, Pseudomonas aeruginosa, Shewanella oneidensis and Methylomicrobium alcaliphilum, characterizing their structures by electron cryotomography and finding evidence that they function in a stress response pathway. Using bioinformatics, we trace the evolution of the system through γ-Proteobacteria, pinpointing key evolutionary events that led to the machine now seen in E. coli. Our results suggest that two ancient chemosensory systems with different inputs and outputs (F6 and F7) existed contemporaneously, with one (F7) ultimately taking over the inputs and outputs of the other (F6), which was subsequently lost.


Asunto(s)
Sustancias Macromoleculares/química , Methylococcaceae/fisiología , Pseudomonas aeruginosa/fisiología , Shewanella/fisiología , Vibrio cholerae/fisiología , Evolución Biológica , Quimiotaxis , Biología Computacional , Tomografía con Microscopio Electrónico , Escherichia coli/fisiología , Proteínas de Escherichia coli , Flagelos/fisiología , Gammaproteobacteria/fisiología , Genoma Bacteriano , Proteínas Quimiotácticas Aceptoras de Metilo/química , Filogenia
6.
Curr Opin Struct Biol ; 52: 64-70, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30223223

RESUMEN

The need for bacteria to interact with their environment has driven the evolution of elaborate secretion systems. By virtue of their function, secretion systems are macromolecular complexes associated with the cell envelope and therefore inherently difficult to study by conventional structural biology techniques. Cryo-electron microscopy (cryoEM) has become an invaluable technique to study large membrane-embedded complexes and led to major advances in the mechanistic understanding of secretion systems. CryoEM comprises of two main modalities, namely single particle analysis and tomography. Here, we review how detailed structures retrieved by single particle analysis combine elegantly with tomography experiments in which the secretion systems are observed in their native cellular context.


Asunto(s)
Sistemas de Secreción Bacterianos , Microscopía por Crioelectrón , Microscopía por Crioelectrón/métodos , Sustancias Macromoleculares/química , Sustancias Macromoleculares/metabolismo , Proteínas de Transporte de Membrana/química , Proteínas de Transporte de Membrana/metabolismo , Modelos Biológicos
7.
Ultramicroscopy ; 190: 1-11, 2018 07.
Artículo en Inglés | MEDLINE | ID: mdl-29655973

RESUMEN

Electron cryotomography is able to visualize macromolecular complexes in their cellular context, in a frozen-hydrated state, and in three dimensions. The method, however, is limited to relatively thin samples. Cryo-focused ion beam (FIB) milling is emerging as a powerful technique for sample thinning prior to cryotomography imaging. Previous cryo-FIB milling reports utilized custom-built non-standard equipment. Here we present a workflow and the required commercially available instrumentation to either implement the method de novo, or as an upgrade of pre-existing dual beam milling instruments. We introduce two alternative protocols and the respective sample holders for milling. The "bare grid holder" allows for milling on plain grids, having the advantage of enabling relatively shallow milling angles for wedge geometries. The "Autogrid holder" is designed for milling grids clamped into a mechanical support ring (Autogrid), resulting in increased stability for lamella geometries. We applied the workflow to prepare samples and record high-quality tomograms of diverse model organisms, including infected and uninfected HeLa cells, amoebae, yeast, multicellular cyanobacteria, Pseudomonas aeruginosa and Escherichia coli cells. The workflow will contribute to the dissemination of electron cryotomography of cryo-FIB milled samples in the biological sciences.


Asunto(s)
Microscopía por Crioelectrón/métodos , Tomografía con Microscopio Electrónico/métodos , Iones/química , Línea Celular Tumoral , Electrones , Células HeLa , Humanos , Microscopía Electrónica de Transmisión/métodos , Flujo de Trabajo
8.
Chem Commun (Camb) ; 50(28): 3722-4, 2014 Apr 11.
Artículo en Inglés | MEDLINE | ID: mdl-24577244

RESUMEN

A supramolecular hydrogel formed by dipeptide Gly-Ala linked with biphenyl-substituted tetrazole serves not only as a 3D matrix for live cells, but also as a carrier to deliver microRNA into the encapsulated cells.


Asunto(s)
Hidrogeles/administración & dosificación , MicroARNs/administración & dosificación , Supervivencia Celular/efectos de los fármacos , Dipéptidos/química , Células Hep G2 , Humanos , Hidrogeles/química , MicroARNs/química , Tetrazoles/química
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