[Individualized treatment of bilateral carotid body tumor].

Objective: To investigate individualized therapeutic strategy for bilateral carotid body tumors. Methods: Clinical data of 16 patients with bilateral carotid body tumor treated from January 2003 to August 2016 were retrospectively studied. Of the 16 patients, 9 were males and 7 were females; 5 were sporadic and 11 were familial; 8 cases were observed, 1 cases was malignant and treated with chemotherapy, and 7 cases were treated with surgery. The treatment course, perioperative complications and clinical efficacy were assessed. Comprehensive evaluation of bilateral carotid body tumors was performed based on the size of bilateral tumor, clinical manifestations, genetic tests and other indicators. Individual treatment strategies included observation, surgery and observation, bilateral surgery, radiotherapy or chemotherapy. Surgical resection of carotid body tumor was unilateral in 3 cases and bilateral in 3 cases; removal of bilateral carotid body tumors plus unilateral jugular bulb in 1 case; and the internal carotid artery was reconstructed with autologous greater saphenous vein in 1 case. Results: All patients were followed up for 3 months to 12 years. There was no patient death during perioperative period. Superior laryngeal nerve injury occurred in 2 case. Baroreceptor failure syndrome occurred in one patient, but it gradually recoverd with medical treatments. Conlusion: It is important to identify whether bilateral carotid body tumors are hereditary and to make an individualized therapeutic strategy for each patient with bilateral carotid body tumors, focusing on the improvement in the quality of life of patient.

Swimming intervention mitigates HFD-induced obesity of rats through PGC-1α-irisin pathway.

OBJECTIVE: Irisin, a newly discovered myokine, can drive the browning of white adipocytes to control body weight or mitigate obesity progression through regulating energy metabolism. However, the underlying mechanisms or specific signal pathways of exercise-induced irisin on the management of obesity are still unclear. MATERIALS AND METHODS: Totally 30 rats were subjected to high fat diet (HFD) feeding for 8 weeks to establish the rat model with obesity successfully. HFD-induced obese model rats were provided with 8 weeks swimming intervention at moderate intensity for exploring the treatment of obesity through exercise intervention. In addition, another 15 rats were subjected to HFD feeding coupled with total 16 weeks swimming intervention at a moderate intensity from the beginning of the experiment, which was used for exploring the prevention of obesity through exercise intervention. Blood and gastrocnemius samples were harvested from obese rats after swimming intervention to explore its specific signal pathways through ELISA analysis and Western blotting. RESULTS: HFD feeding of rats for 8 weeks could lead to the obesity due to the disorders of lipid metabolism. Totally 8 weeks swimming intervention at moderate intensity for rats with obesity could obviously alleviate the progression of obesity and 16 weeks swimming intervention from the beginning of the experiment could significantly inhibit the development of obesity. Meanwhile, swimming intervention could result in an increased phosphorylation of AMPK and up-regulation of irisin and PGC-1α as the biomarkers of energy metabolism. CONCLUSION: Exercise intervention can activate PGC-1α-dependent irisin to induce the browning of white adipocytes, thus inhibiting or alleviating the occurrence and development of obesity.

Effects of high-intensity treadmill training on timeliness and plasticity expression of irisin in mice.

OBJECTIVE: The purpose of this study was to investigate effects of high-intensity aerobic exercise training on timeliness and plasticity expression of irisin in mice and change of FNDC5, ACCß expression, and to explore possible ways to influence its mechanism of fatty acid metabolism. MATERIALS AND METHODS: Adult male mice of specific pathogen-free grade [Kunming mice, (20 ± 2) g] are randomly divided into 4 groups. Wherein the first group is immediately after one-time exercise groups: including control group (CN group 1), 0.5 h exercise group (group 2), 1 h exercise group (group 3), 1.5 h exercise group (group 4) and 2 h exercise group (group 5), each for 10. The second group is rest after one-time 60 min exercise groups: including control group (CN group 1), rest 20 min groups (groups 2), rest 40 min group (group 3), rest 60 min group (groups 4), rest 80 min group (group 5), each for 10. Third group is immediately after long-term exercise groups: including the control group (CN group 1), 0.5 h exercise group (group 2), 1 h exercise group (group 3), 1.5 h exercise group (group 4) and 2 h exercise group (group 5), each for 10. The fourth group is rest after long-term 60 min exercise group: including control group (CN group 1), rest 20 min group (group 2), rest 40 min group (group 3), rest 60 min group (4 groups) and rest 80 min groups (5 groups), each for 10. RESULTS: With the extension of a one-time high-intensity exercise time, the mouse FNDC5 protein, P-ACCß / ACCß ratio showed fluctuations, and opposite trends between the two, its turning points are 1.5 h; FNDC5 protein and P-ACCß / ACCß ratio with long-term exercise in mice at different time produce adaptability; the regulation of exercise induced irisin timeliness and plasticity reflected after a long-term exercise irisin expression in serum showed a steady decline in trend and return to normal levels, compared to a one-time exercise, expression of irisin is more stable. CONCLUSIONS: With the high-intensity exercise a one-time extension of time, the mouse FNDC5 proteins, P-ACCß / ACCß ratio showed fluctuations, and both changes in the opposite trend, its turning points are 1.5 h; the long-term exercise can produce FNDC5 proteins, P-ACCß / ACCß ratios adaptable, more stable expression of the irisin curve after long-term exercise compared to a one-time exercise.