[MVT - A Multiprofessional Behavioural Therapy Program for Inpatient Treatment of Depression in Old Age]. / MVT - ein Multiprofessionelles VerhaltensTherapeutisches Programm zur stationären Behandlung von Depressionen im höheren Lebensalter.

OBJECTIVE: Depression in old age is common but patients are rarely treated in specialized units implementing a psychotherapeutic treatment approach. METHODS: A multiprofessional behavioral therapy program (MVT) for inpatient treatment of depressive elderlies was conceptualized, implemented and evaluated at a specialized unit of a hospital for psychiatry and psychotherapy. RESULTS: Preliminary analyses indicated that various behavioral group interventions were well accepted by patients. CONCLUSIONS: The implementation of a psychotherapeutic therapy program specifically designed for depressed elderly inpatients is feasible and could be more broadly applied to improve clinical practice for this patient group.

HOPE--a new fixing technique enables preservation and extraction of high molecular weight DNA and RNA of > 20 kb from paraffin-embedded tissues. Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect.

The growing number of molecular pathologic tools that are currently available require material with good long term preservation of morphology, nucleic acids, and antigenic structures. However, pathologic investigations of tissues done at a molecular level are often hampered by the fixatives in use. We thus endeavored to design a new fixing system, including subsequent paraffin-embedding and sectioning, that makes complete pathologic analyses possible, with special consideration of immunohistochemistry (IHC), in situ hybridization (ISH), and molecular pathology. The optimized HOPE (Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect) fixing technique allows us to preserve and extract high molecular weight DNA and RNA of > 20 kbp suitable for downstream applications, such as PCR and RT-PCR from HOPE-fixed, paraffin-embedded tissues that are up to 5 years old. This technique will most probably lead to new impacts on molecular pathology.