Removal of fluoroquinolone antibiotic and sulfonated dye by functionalized Persea americana seed powder: Appraisal on phase transfer kinetics, equilibrium, economics, and applications in rural settings.

The study focuses on reactive orange 16 (RO16), a sulfonated dye, and ciprofloxacin (CiP), a fluoroquinolone antibiotic treatment from aquatic surface by adsorption. The functionalized Persea americana seed powder (PASP) was developed by acid hydrolysis technique and investigated for RO16 and CiP removal in batch scale at different concentrations for CiP and RO16, pH (2-8), contact duration and temperature (303-318K). Utilizing a scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy (EDAX), the generated native PASP were assessed for their morphological characteristics. Fourier transform infrared (FTIR) spectroscopy was applied to examine the performing characteristics of PASP. Experimental findings with four kinetic mathematical models allowed the estimation of the process involved in the biosorption. The most effective agreement was explained by the pseudo-second-order model and Sips isotherm (Cip = 34.603 mg/g and RO16 = 30.357 mg/g) at 303K temperature. For Cip Process economics of the biosorbent was done, and it was observed that it was less than the readily market-available activated carbon.

Titanium dioxide based nanocomposites - Current trends and emerging strategies for the photocatalytic degradation of ruinous environmental pollutants.

Many ruinous pollutants are omnipresent in the environment and among them; pesticides are xenobiotic and pose to be a bio-recalcitrance. Their detrimental ecological and environmental impacts attract attention of environmental excerpts and the surge of stringent regulations have endows the need of a technically feasible treatment. This critical review emphasizes about the occurrence, abundance and fate of structurally distinct pesticides in different environment. The practiced remedial strategies and in particular, the advanced oxidation processes (AOPs) those utilize the photo-catalytic properties of nano-composites for the degradation of pollutants are critically discussed. Photo-catalytic degradation utilizes many composite materials at nano-scale level, wherein synthesis of nano-composites with appropriate precursors and other adjoining functional moieties are of prime importance. Therefore, suitable starter materials along with the reaction conditions are prerequisite for effectively tailoring the nano-composites. The aforementioned aspects and their customized applications are critically discussed. The associated challenges, opportunities and process economics of degradation using photo-catalytic AOP techniques are highlighted and in addition, the review tries to explain how best the photo-degradation can be a stand-alone tool with a societal importance. Conclusively, the future prospects for undertaking new researches in photo-catalytic breakdown of pollutants that can be judiciously sustainable.

Remedial strategies for abating 1,4-dioxane pollution-special emphasis on diverse biotechnological interventions.

1,4-dioxane is a heterocyclic ether used as a polar industrial solvent and are released as waste discharges. 1,4-dioxane deteriorates health and quality, thereby attracts concern by the environment technologists. The need of attaining sustainable development goals have resulted in search of an eco-friendly and technically viable treatment strategy. This extensive review is aimed to emphasis on the (a) characteristics of 1,4-dioxane and their occurrence in the environment as well as their toxicity, (b) remedial strategies, such as physico-chemical treatment and advanced oxidation techniques. Special reference to bioremediation that involves diverse microbial strains and their mechanism are highlighted in this review. The role of macronutrients, stimulants and other abiotic cofactors in the biodegradation of 1,4-dioxane is discussed lucidly. We have critically discussed the inducible enzymes, enzyme-based remediation, distinct instrumental method of analyses to know the fate of intermediates produced from 1,4-dioxane biotransformation. This comprehensive survey also tries to put forth the different toxicity assessment tools used in evaluating the extent of detoxification of 1,4-dioxane achieved through biotransforming mechanism. Conclusively, the challenges, opportunities, techno-economic feasibility and future prospects of implementing 1,4-dioxane through biotechnological interventions are also discussed.

Response surface algorithm for improved biotransformation of 1,4-dioxane using Staphylococcus capitis strain AG.

The present investigation reports the biotransformation of an endrocrine disrupting agent; 1,4-dioxane through bacterial metabolism. Initially, potential bacterial isolates capable of surviving with minimum 1,4-dioxane were screened from industrial wastewater. Thereafter, screening was done to isolate a bacteria which can biotransform higher concentration (1000 mg/L) of 1,4-dioxane. Morphological and biochemical features were examined prior establishing their phylogenetic relationships and the bacterium was identified as Staphylococcus capitis strain AG. Biotransformation experiments were tailored using response surface tool and predictions were made to elucidate the opimal conditions. Critical factors influencing bio-transformation efficiency such as tetrahydrofuran, availability of 1,4-dioxane and inoculum size were varied at three different levels as per the central composite design for ameliorating 1,4-dioxane removal. Functional attenuation of 1,4-dioxane by S. capitis strain AG were understood using spectroscopic techniques were significant changes in the peak positions and chemical shifts were visualized. Mass spectral profile revealed that 1.5 (% v/v) S. capitis strain AG could completely (∼99%) remove 1000 mg/L 1,4-dioxane, when incubated with 2 µg/L tetrahydrofuran for 96 h. The toxicity of 1,4-dioxane and biotransformed products by S. capitis strain AG were tested on Artemia salina. The results of toxicity tests revealed that the metabolic products were less toxic as they exerted minimal mortality rate after 48 h exposure. Thus, this research would be the first to report the response prediction and precise tailoring of 1,4-dioxane biotransformation using S. captis strain AG.

Remediation of phthalate acid esters from contaminated environment-Insights on the bioremedial approaches and future perspectives.

Phthalates are well-known emerging pollutants that are toxic to the environment and human health. Phthalates are lipophilic chemicals used as plasticizers in many of the items for improving their material properties. These compounds are not chemically bound and are released to the surroundings directly. Phthalate acid esters (PAEs) are endocrine disruptors and can interfere with hormones, which can cause issues with development and reproduction, thus there is a huge concern over their existence in various ecological surroundings. The purpose of this review is to explore the occurrence, fate, and concentration of phthalates in various environmental matrices. This article also covers the phthalate degradation process, mechanism, and outcomes. Besides the conventional treatment technology, the paper also aims at the recent advancements in various physical, chemical, and biological approaches developed for phthalate degradation. In this paper, a special focus has been given on the diverse microbial entities and their bioremedial mechanisms executes the PAEs removal. Critically, the analyses method for determining intermediate products generated during phthalate biotransformation have been discussed. Concluisvely, the challenges, limitations, knowledge gaps and future opportunities of bioremediation and their significant role in ecology have also been highlighted.

Conversion of solid wastes and natural biomass for deciphering the valorization of biochar in pollution abatement: A review on the thermo-chemical processes.

This overview addresses the formation of solid trash and the various forms of waste from a variety of industries, which environmentalists have embraced. The paper investigates the negative effects on the environment caused by unsustainable management of municipal solid trash as well as the opportunities presented by the formal system. This examination looks at the origins of solid waste as well as the typical treatment methods. Pyrolysis methods, feedstock pyrolysis, and lignocellulosic biomass pyrolysis were highlighted. Explain in detail the various thermochemical processes that take place during the pyrolysis of biomass. Due to its carbon content, low cost, accessibility, ubiquitousness, renewable nature, and environmental friendliness, biomass waste is a unique biochar precursor. This study looks at the different types of biomass waste that are available for treating wastewater. This study discussed a wide variety of reactors. Adsorption is the standard method that is used the most frequently to remove hazardous organic, dye, and inorganic pollutants from wastewater. These pollutants cause damage to the environment and water supplies, thus it is important to remove them. Adsorption is both simple and inexpensive to utilize. Temperature-dependent conversions explain the kinetic theories of biomaterial biochemical degradation. This article presents a review that explains how pyrolytic breakdown char materials can be used to reduce pollution and improve environmental management.

Environmental health and risk assessment metrics with special mention to biotransfer, bioaccumulation and biomagnification of environmental pollutants.

The environment pollutants, which are landed up in environment because of human activities like urbanization, mining and industrializations, affects human health, plants and animals. The living organisms present in environment are constantly affected by the toxic pollutants through direct contact or bioaccumulation of chemicals from the environment. The toxic and hazardous pollutants are easily transferred to different environmental matrices like land, air and water bodies such as surface and ground waters. This comprehensive review deeply discusses the routes and causes of different environmental pollutants along with their toxicity, impact, occurrences and fate in the environment. Environment health and risk assessment tools that are used to evaluate the harmfulness, exposure of living organisms to pollutants and the amount of pollutant accumulated are explained with help of bio-kinetic models. Biotransfer, toxicity factor, biomagnification and bioaccumulation of different pollutants in the air, water and marine ecosystems are critically addressed. Thus, the presented survey would be collection of correlations those addresses the factors involved in assessing the environmental health and risk impacts of distinct environmental pollutants.

Conductive polymer layered semiconductor for degradation of triclopyr acid and 2,4-dichlorophenoxyacetic acid from aqueous stream using coalesce adsorption-photocatalysis technique.

Polyaniline supported titanium dioxide nanoparticles (PTs) were fabricated using chemical oxidative aniline polymerization in the presence of titanium dioxide with ammonium peroxydisulfate as an oxidant. The synthesized PTs were thoroughly characterized for their morphological and functional features. PTs were employed for the photodegradation of acidic herbicides; 2,4-dichlorophenoxyacetic acid (2,4-D) and triclopyr acid (TCP). PT's surface modifications were imparted and their herbicide removal efficiencies were compared. The best operating conditions for adsorption/photocatalysis were 0.5 g/L photocatalyst, 10 mg/L concentration of individual herbicides resulted in 90.72% removal of TCP at pH 4 and 99.91% removal of 2,4-D at pH 5. Adsorption kinetics of herbicides, onto PT-1 showed the equilibrium attainment within 30 min and experimental data obeyed pseudo-second order model for TCP and 2,4-D removal which was governed by chemisorption. Analysis of TCP and 2,4-D adsorption indicated that the removal followed Sips model for TCP removal while Redlich-Peterson model explained the removal of 2,4-D by PT-1. Rate constants indicated that the amount of TiO2 in the PTs played an important role in removing the herbicides and PT-1 material excellent remarkable activity for three cycles of photodegradation. Thus, this work reports the polymerization of aniline onto TiO2 and their utility as photocatalyst for the expulsion of 2,4-D and TCP from water.

Isolation and screening of sulfur-oxidizing bacteria from coast of Bhavnagar, India, and formulation of consortium for bioremediation.

Reduced sulfur compounds are a nuisance in coastal industries causing heavy economical as well as ecological loss. One such compound, hydrogen sulfide, is proven toxic to aquatic animals as it interferes with their respiration and metabolism as well as overall development, thereby causing direct increase in mortality. Typically, 96-h LC50 values to freshwater and marine fishes are 0-25µM and 525-700µM, respectively. Management of sulfide and other reduced sulfur compounds from aquaculture water and sediment using bioremediating sulfur-oxidizing bacteria as probiotics has attracted attention in recent decades due to its efficiency and minimized environmental effects. In the present study, 201 native and indigenous probiotic candidates were isolated, from various coastal environments. The prospective candidates were screened based on pH reduction and 19 sulfur-oxidizing bacteria were selected and tested for salt tolerance. Further screening was done based on biosafety, ability to produce sulfate by oxidizing thiosulfate, and 16S rRNA-based identification to obtain nine probiotic candidates. Three strains (Enterobacter ludwigii HS1-SOB, Pseudomonas stutzeri B6-SOB, and Cytobacillus firmus C8-SOB) exerting highest sulfate-ion production were selected for formulating a probiotic consortium using mixture design matrix. The optimal composition was determined to be equal ratios of the three isolates that yielded 0.083 mM of sulfate from thiosulfate broth medium at room temperature in 7 days. This is a standalone report of sulfur-oxidizing probiotic consortium composed of the said bacteria. The consortium may be used as a strong tool for remediation of reduced sulfur in aquaculture and associated coastal environments.

Chemo-metric engineering designs for deciphering the biodegradation of polycyclic aromatic hydrocarbons.

Polycyclic aromatic hydrocarbons (PAHs) are non-polar organic compounds that are omnipresent in the environment and released due to anthropogenic activities through emissions and discharges. PAHs, being xenobiotic and exerts health impacts, thus they attract serious concern by the environmentalists. The stringent regulations and the need of sustainable development urges the hunt for a technically feasible and cost-effective wastewater treatment. Although the conventional physico-chemical treatment are widely preferred, they cause secondary pollution problems and demand subsequent treatment options. This comprehensive review intends to address the (a) different PAHs and their associated toxicity, (b) the remedial strategies, particularly biodegradation. The biological wastewater treatment techniques that involve microbial systems are highly influenced by the different physio-chemical and environmental parameters. Therefore, suitable optimization techniques are prerequisite for effective functioning of the biological treatment that sustains judiciously and interpreted in a lesser time. Here we have aimed to discuss (a) different chemo-metric tools involved in the design of experiments (DoE), (b) design equations and models, (c) tools for evaluating the model's adequacy and (d) plots for graphically interpreting the chemo-metric designs. However, to best of our knowledge, this is a first review to discuss the PAHs biodegradation that are tailored by chemo-metric designs. The associated challenges, available opportunities and techno-economic aspects of PAHs degradation using chemo-metric engineering designs are explained. Additionally, the review highlights how well these DoE tools can be suited for the sustainable socio-industrial sectors. Concomitantly, the futuristic scope and prospects to undertake new areas of research exploration were emphasized to unravel the least explored chemo-metric designs.

Evaluation of phase transfer kinetics and thermodynamic equilibria of Reactive Orange 16 sorption onto chemically improved Arachis hypogaea pod powder.

Biosorbent from pods of Arachis hypogaea (AhP) were inducted with sulphuric acid treatment and then the activated materials were employed to sequester a sulphonated textile dye; Reactive Orange 16 (RO16) from water system. The characteristic features of the surface functionalized AhP (Ct-AhP) were analysed using instrumentation techniques. The biosorption influencing variables like operating pH, agitating time, initial RO16 concentration and temperature effects were investigated. One-factor optimization revealed that 0.5 g Ct-AhP was sufficient to achieve maximum removal of RO16 (20-120 mg/L) within 180 min agitation at 150 rpm. The isotherm data were applied to non-linear isotherms viz., Freundlich, Langmuir and Temkin models as well as rate limiting steps were elucidated using kinetic models. Freundlich isotherm showed good fit and pseudo-second order kinetic data explained RO16 removal by Ct-AhP followed chemisorption. The outcome of thermodynamic parametric values infer that RO16 biosorption was spontaneous, feasible and involved exothermic type of heat. Elovich and intraparticle diffusion revealed the biosorption mechanisms. The maximum RO16 biosorption (56.48 mg/g) by 0.5 g Ct-AhP were witnessed in the system containing 120 mg/L RO16 agitated at 150 rpm operating at pH 7.0, 303 K for a span of 180 min. Thus, the Ct-AhP is considered to be a promising biosorbent which can be employed in treating the textile effluents.

Kinetic modelling of high turbid water flocculation using native and surface functionalized coagulants prepared from shed-leaves of Avicennia marina plants.

Coagulation performance of shed-leaves of Avicennia marina plants collected from Alang coastline, Gujarat (India) was scrutinized for the treatment of mud and starch water suspensions. For which, native, hydrochloric acid, sodium hydroxide and sodium chloride treated A. marina shed-leaves were processed with minimum environmental impact. Experiments were accomplished for the concentration of water suspensions (10-50 g/L) at the range of pH 7.0-8.0. The performances of these coagulants were assessed in terms of reduction in turbidity, pH, alkalinity, hardness, electrical conductivity and solids from water suspensions. The removal of bulk impurities was noted due to the floc formation of coagulant through hydrolysing salts, thus, resulted in the highest settlement at pH 7.82, 7.90 for mud and starch water, accordingly. Native and functionalized A. marina coagulants (AMCs) were characterized and interpreted using scanning electron microscopy, elemental analyses, energy dispersive and Fourier transform infrared spectroscopy. HCl treated AMC was relatively effective with good coagulation performance (96.76%), when compared with native and other treated AMCs. The turbidity removal by all AMCs obeyed with World Health Organization (WHO) acceptable limit of finished water, where HCl treated AMC clarified 15.15 and 16.36 NTU of mud and starch water suspensions to produce a clear water of 0.92 and 1.61 NTU, respectively. The proficiency of prepared AMCs were compared with other natural coagulants and surface functionalized (HCl > NaOH > NaCl) AMCs prepared in this study exerted better performance than the native AMC. The critical coagulation rate from the second-order kinetics were evaluated and the results were highly satisfying. Other physico-chemical parameters of water suspensions were evident for the adequate removal of impurities by non-toxic plant-based coagulants.

Chemo-metrically formulated consortium with selectively screened bacterial strains for ameliorated biotransformation and detoxification of 1,4-dioxane.

The biotransformation of 1,4-dioxane, a endrocrine disrupting chemical was achieved using different bacterial strains and their consortia. Three different bacterial isolates were screened on their ability to grow with 50 mg/L 1,4-dioxane in the basal mineral medium. Then the isolates were tested for its efficiency to biotransform 1000 mg/L 1,4-dioxane at varying period of time; 24-120 h. The isolates were distinguished by their morphological features and 16 S rRNA gene sequencing was done to evaluate the phylogenetic relationships. The isolates were identified as Bacillus marisflavi strain MGA, Aeromonas hydrophila strain AG and Shewanella putrefaciens strain AG. The degree of biotransformation was escalated by constructing a bacterial consortium using statistical tool; response-mixture matrix under the design of experiments. The fully grown bacterial strains were used as ingredients in different proportions to formulate the consortium. The biotransformation was analyzed for functional attenuation using spectroscopic techniques and reduction in 1,4-dioxane level was confirmed using mass spectrometry. The precise quantification of biotransformation using mass spectral profile revealed that the consortium removed 31%, 61% and 85% of 1000 mg/L 1,4-dioxane within 96, 120 and 144 h respectively. The activities of inducible laccase were elucidated during biotransformation of 1,4-dioxane. Bio-toxicity of treated and untreated 1,4-dioxane on brine shrimp; Artemia salina showed that the biotransformed products were less toxic. Therefore, this report would be first of its kind to report the biotransformation and detoxification of 1,4-dioxane by a statistically designed bacterial consortium.

Ameliorating process parameters for zeaxanthin yield in Arthrobacter gandavensis MTCC 25325.

The present study aims to escalate the production of prophylactic agent zeaxanthin using a screened potential bacterial isolate. For this purpose, a freshwater bacterium capable of producing zeaxanthin was isolated from Bor Talav, Bhavnagar. The 16S rRNA sequence confirmed the isolate as Arthrobacter gandavensis. The bacterium was also submitted to Microbial Type Culture Collection, CSIR-Institute of Microbial Technology, Chandigarh, India, with the accession number MTCC 25325. The chemo-metric tools were employed to optimise the influencing factors such as pH, temperature, inoculum size, agitation speed, carbon source and harvest time on zeaxanthin yield. Thereafter, six parameters were narrowed down to three factors and were optimised using the central composite design (CCD) matrix. Maximum zeaxanthin (1.51 mg/g) was derived when A. gandavensis MTCC 25325 was grown under pH 6.0, 1.5% (w/v) glucose and 10% (v/v) inoculum size. A high regression coefficient (R2= 0.92) of the developed model indicated the accurateness of the tested parameters. To the best of our knowledge, this is the first report on tailoring the process parameters using chemo-metric optimisation for escalating the zeaxanthin production by A. gandavensis MTCC 25325.

Characterization, analysis, and application of fabricated Fe3O4-chitosan-pectinase nanobiocatalyst.

The investigation on fabrication of Fe3O4-chitosan-pectinase nanobiocatalyst was performed by covalently binding the pectinase onto carboxyl group activated chitosan-coated magnetic nanoparticles (CMNPs). The morphological and size distribution analysis of the different magnetic nanoparticles (MNPs) was done using transmission electron microscopy (TEM), and the average diameter was 11.07 ± 3.04, 11.55 ± 3.16, and 11.59 ± 3.16 nm for MNPs, CMNPs, and fabricated nanobiocatalyst, respectively, suggesting that there was no significant change in the size of MNPs after coating and binding. The characteristic peaks occurred at 2θ of 30.39, 35.43, 43.37, 57.22, and 62.9, and their corresponding indices 220, 311, 400, 520, and 441 for different MNPs from the X-ray diffraction (XRD) studies confirmed the presence of Fe3O4 with the spinel structure, and there was no phase change even after coating and binding. The various required characteristic absorption peaks (575, 585, 1,563, 1,614, 1,651, and 1,653 cm(-1)) from Fourier transform infrared (FT-IR) spectroscopy confirmed the surface modifications and binding of pectinase onto the MNPs. At the weight ratio of about 19.8 × 10(-3) mg bound pectinase/mg activated CMNPs, the activity of fabricated nanobiocatalyst was found to be maximum. In order to monitor their improved activity, the pH, temperature, reusability, storage ability, and kinetic studies were established.

Fabrication, characterization and application of pectin degrading Fe3O4-SiO2 nanobiocatalyst.

The covalent binding of pectinase onto amino functionalized silica-coated magnetic nanoparticles (CSMNPs) through glutaraldehyde activation was investigated for nanobiocatalyst fabrication. The average particle size and morphology of the nanoparticles were characterized using transmission electron microscopy (TEM). The statistical analysis for TEM image suggests that the coating and binding process did not cause any significant change in size of MNPs. The morphological and phase change of the magnetic nanoparticles (MNPs) after various coatings and immobilization were characterized by X-ray diffraction (XRD) studies. The various surface modifications and pectinase binding onto nanoparticles were confirmed by Fourier transform infrared (FT-IR) spectroscopy. The maximum activity of immobilized pectinase was obtained at its weight ratio of 19.0×10(-3) mg bound pectinase/mg CSMNPs. The pH, temperature, reusability, storage ability and kinetic studies were established to monitor their improved stability and activity of the fabricated nanobiocatalyst. Furthermore, the application was extended in the clarification of Malus domestica juice.

Chemometric formulation of bacterial consortium-AVS for improved decolorization of resonance-stabilized and heteropolyaromatic dyes.

A bacterial consortium-AVS, consisting of Pseudomonas desmolyticum NCIM 2112, Kocuria rosea MTCC 1532 and Micrococcus glutamicus NCIM 2168 was formulated chemometrically, using the mixture design matrix based on the design of experiments methodology. The formulated consortium-AVS decolorized acid blue 15 and methylene blue with a higher average decolorization rate, which is more rapid than that of the pure cultures. The UV-vis spectrophotometric, Fourier transform infra red spectrophotometric and high performance liquid chromatographic analysis confirm that the decolorization was due to biodegradation by oxido-reductive enzymes, produced by the consortium-AVS. The toxicological assessment of plant growth parameters and the chlorophyll pigment concentrations of Phaseolus mungo and Triticum aestivum seedlings revealed the reduced toxic nature of the biodegraded products.