RESUMEN
A series of 23 nitroxides () was tested for biofilm modulatory activity using a crystal violet staining technique. 3-(Dodecane-1-thiyl)-4-(hydroxymethyl)-2,2,5,5-tetramethyl-1-pyrrolinoxyl () was found to significantly suppress biofilm formation and elicit dispersal events in both Pseudomonas aeruginosa and mixed-culture biofilms. Twitching and swarming motilities were enhanced by nitroxide , leaving the planktonic-specific swimming motility unaffected and suggesting that the mechanism of -mediated biofilm modulation is linked to the hyperactivation of surface-associated cell motilities. Preliminary structure-activity relationship studies identify the dodecanethiyl chain, hydroxymethyl substituent and the free radical moiety to be structural features pertinent to the anti-biofilm activity of .
Asunto(s)
Antibacterianos/síntesis química , Óxidos de Nitrógeno/química , Pseudomonas aeruginosa/metabolismo , Antibacterianos/química , Biopelículas , Movimiento Celular , Relación Dosis-Respuesta a Droga , Diseño de Fármacos , Espectroscopía de Resonancia por Spin del Electrón , Radicales Libres , Violeta de Genciana/química , Espectroscopía de Resonancia Magnética , Óxido Nítrico/química , Presión , Relación Estructura-ActividadRESUMEN
3-(Dodecanethiyl)-4-(hydroxymethyl)-2,2,5,5-tetramethyl-1-pyrrolinoxyl (5) effectively disperses biofilms of relevance to cultural materials while preventing their formation.