RESUMEN
OBJECTIVE: This study aimed to detect novel mesenchymal stem cell peptides/biomarkers of bronchopulmonary dysplasia (BPD) in the tracheal aspirate fluid (TAF) of preterm infants. STUDY DESIGN: Participants included infants less than 32 weeks' gestational age or birth weight under 1500 grams who required endotracheal intubation and mechanical ventilation within first 24 hours of life. TAF sample collection was performed at the time of the first clinically indicated routine suctioning. Standardization curves for human levels of osteopontin (Opn), macrophage colony stimulating factor 1 (Csf1), transforming growth factor beta 1 (TGF-ß1), and secretory immunoglobulin A (sIgA) were generated for 15 enrolled participants. RESULTS: We demonstrated that stem cell biomarkers are secreted into the TAF of preterm infants and their concentrations can be easily measured during the first week of life. CONCLUSIONS: Further studies are warranted to determine a causal relationship between these biomarkers and BPD development and severity.
Asunto(s)
Inmunoglobulina A/metabolismo , Factor Estimulante de Colonias de Macrófagos/metabolismo , Células Madre Mesenquimatosas/metabolismo , Osteopontina/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo , Biomarcadores/metabolismo , Peso al Nacer , Líquidos Corporales/metabolismo , Displasia Broncopulmonar/metabolismo , Estudios de Factibilidad , Femenino , Edad Gestacional , Humanos , Recién Nacido , Recien Nacido Prematuro , Intubación Intratraqueal , Masculino , Proyectos Piloto , Respiración Artificial , TráqueaRESUMEN
Transfusion-related acute lung injury (TRALI) is a life-threatening complication of blood product transfusion characterized by sudden onset hypoxemic respiratory failure with bilateral lung infiltrates and non-cardiogenic pulmonary edema developing within 6 hours of transfusion. It is believed to be under-recognized, particularly among preterm neonates in whom co-existing developmental lung disease adds diagnostic complexity. Here we report the case of a preterm neonate who developed TRALI during a blood transfusion following PDA ligation.