RESUMEN
Organically-modified siloxanes were used as host materials to examine the influence of surface chemistry on protein conformation in a crowded environment. The sol-gel materials were prepared from tetramethoxysilane and a series of monosubstituted alkoxysilanes, RSi(OR')(3), featuring alkyl groups of increasing chain length in the R-position. Using circular dichroism spectroscopy in the far-UV region, apomyoglobin was found to transit from an unfolded state to a native-like helical state as the content of the hydrophobic precursor increased from 0 to 15%. At a fixed molar content of 5% RSi(OR')(3), the helical structure of apomyoglobin increased with the chain length of the R-group, i.e. methylAsunto(s)
Interacciones Hidrofóbicas e Hidrofílicas
, Proteínas/química
, Dióxido de Silicio/química
, Dicroismo Circular
, Anteojos
, Microscopía de Fuerza Atómica
, Microscopía Electrónica de Rastreo
, Compuestos Orgánicos/química
, Porosidad