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1.
Zhonghua Yan Ke Za Zhi ; 59(7): 575-580, 2023 Jul 11.
Artículo en Zh | MEDLINE | ID: mdl-37408430

RESUMEN

In addition to the abnormalities of the extraocular muscles themselves and the nervous system innervating the extraocular muscles, the tissue pulley abnormalities around the extraocular muscles may lead to the occurrence of strabismus. In recent years, researchers have shown the intramuscular nerve distribution of the extraocular muscles using the Sihler technique. With the continuous progress of imaging technology, magnetic resonance imaging, computed tomography, anterior segment optical coherence tomography and ultrasound biomicroscopy can be used to observe the attachment sites of the extraocular muscles. This review summarizes the latest research progress on the neuroanatomy of the extraocular muscles, in order to provide reference for the clinical diagnosis and treatment of strabismus.


Asunto(s)
Músculos Oculomotores , Estrabismo , Humanos , Músculos Oculomotores/patología , Estrabismo/diagnóstico , Imagen por Resonancia Magnética , Tomografía de Coherencia Óptica/métodos , Tomografía Computarizada por Rayos X
2.
Langmuir ; 34(20): 5807-5812, 2018 05 22.
Artículo en Inglés | MEDLINE | ID: mdl-29694782

RESUMEN

It is generally recognized that superhydrophobic surfaces in water may be used for corrosion resistance due to the entrapped air in the solid/liquid interface and could find potential applications in the protection of ship hull. For a superhydrophobic surface, as its immersion depth into water increases, the resultant hydrostatic pressure is also increased, and the entrapped air can be squeezed out much more easily. It is therefore predicted that high hydrostatic pressure would cause an unexpected decrease in corrosion resistance for the vessels in deep water (e.g., submarines) because of the unstable entrapped air. In this work, in order to clarify the role of hydrostatic pressure in the corrosion behavior of superhydrophobic surfaces, two typical superhydrophobic surfaces (SHSs) were prepared on bare and oxidized aluminum substrates, respectively, and then were immersed into the NaCl aqueous solutions with different depths of ∼0 cm (hydrostatic pressure ∼0 kPa), 10 cm (1 kPa), and 150 cm (15 kPa). It was found out for the SHSs on the oxidized Al, as the hydrostatic pressure increased, the corrosion behavior became severe. However, for the SHSs on the bare Al, their corrosion behavior was complex due to hydrostatic pressure. It was found that the corrosion resistance under 1 kPa was the highest. Further mechanism analysis revealed that this alleviated corrosion behavior under 1 kPa resulted from suppressing the oxygen diffusion through the liquid and reducing the subsequent corrosion rate as compared with 0 kPa, whereas the relatively low hydrostatic pressure (HP) could stabilize the entrapped air and hence enhance the corrosion resistance, compared with 15 kPa. The present study therefore provided a fundamental understanding for the applications of SHSs to prevent the corrosion, especially for various vessels in deep water.

3.
Beijing Da Xue Xue Bao Yi Xue Ban ; 50(5): 932-936, 2018 Oct 18.
Artículo en Zh | MEDLINE | ID: mdl-30337762

RESUMEN

Congenital fibrinogen deficiency is an autosomal recessive or dominant disorder in which quantitative (afibrinogenaemia or hypofibrinogenaemia) or qualitative (dysfibrinogenaemia) defects in the fibrinogen Aa, Bb or c protein chains that lead to reduced functional fibrinogen. We now report the perioperative management of 4 pregnant women suffering from hypofibrinogenaemia scheduled for elective caesarean section from December 2012 to October 2016 in Peking University First Hospital and review this disease with reference to classification, symptom, replacement therapy, and selection of the modes of pregnancy termination and anesthesia. The four patients were all asymptomatic, whereas there existed recurrent pregnancy loss (case 3), family history (case 2), and offspring heredity (cases 3 and 4). Routine clotting studies revealed low fibrinogen levels and prolonged thrombin time (TT) during pregnancy and on admission. However, the platelet (PLT) count, prothrombin time (PT) and activated partial thromboplastin time (APTT) were normal. All the patients were administered fibrinogen concentrate perioperatively, and underwent uncomplicated combined spinal-epidural anesthesia and uneventful surgical procedure without postpartum hemorrhage. The replacement therapy of fibrinogen or fresh frozen plasma administration was essential to avoid anesthesia and obstetric complications. Regional blockade could safely be offered in the caesarean section, providing that their coagulation defect was corrected by availability of therapeutic products and adequate response to treatment. In addition, the point-of-care rotational thrombelastometry (ROTEM) or thrombelastogram (TEG) could play an important role in an optimal perioperative management for such patients. Management plans must be tailored to each individual, taking into consideration their bleeding risk as well as potential maternal and neonatal complications.


Asunto(s)
Afibrinogenemia , Cesárea , Complicaciones del Embarazo , Afibrinogenemia/complicaciones , Afibrinogenemia/terapia , Pruebas de Coagulación Sanguínea , Femenino , Fibrinógeno , Humanos , Tiempo de Tromboplastina Parcial , Embarazo , Complicaciones del Embarazo/terapia , Tromboelastografía
4.
Transfus Med ; 27(6): 408-412, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28944508

RESUMEN

BACKGROUND: Garzê and Aba form the second largest Tibetan-inhabited area of China. Blood services have never been reported for this region before. OBJECTIVE: To assess the current situation and analyse whether a safe and adequate blood supply has been developed in both Garzê and Aba. METHODS: We conducted a longitudinal survey covering the period 2011-2016. The subjects of interest were recruited from non-remunerated voluntary donation, blood testing, clinical transfusion practices and infrastructure of local blood service systems. RESULTS: The donation rate and blood collection volume were below the average levels of both the Sichuan Province and mainland China. Component therapy was widely used, but inappropriate usage of whole blood existed. A lack of national specific standards for people on the plateaus led to local blood transfusions being conducted without full clinical assessment. Endemic and frequently occurring disease, such as hydatid disease and gastrointestinal disease, were inevitable risks for blood utilisation and safety. The potential influence of religious belief and traditions, like 'male-leaving marriages', of Tibetans on donor recruitment and blood safety requires further research. CONCLUSIONS: A relatively safe and complete blood service system has been developed in this region. However, there is still an urgent need for comprehensive and effective support from the government in terms of policies and finance. As an epidemic area of hydatid disease and sexually transmitted disease, this region needs to emphasise public health measures, such as blood safety and inappropriate usage of blood products.


Asunto(s)
Donantes de Sangre , Atención a la Salud , Encuestas y Cuestionarios , Atención a la Salud/organización & administración , Atención a la Salud/normas , Femenino , Humanos , Estudios Longitudinales , Masculino , Tibet
5.
Beijing Da Xue Xue Bao Yi Xue Ban ; 49(5): 814-818, 2017 10 18.
Artículo en Zh | MEDLINE | ID: mdl-29045961

RESUMEN

OBJECTIVE: To investigate the effects of the recent upper respiratory tract infections (URI) on the incidence of perioperative respiratory adverse events in children scheduled to undergo general anesthesia and elective surgery. METHODS: In the study, 232 children undergoing general anesthesia with laryngeal mask airway (LMA) for elective ophthalmic surgeries at Peking University First Hospital, Beijing, China, from Nov. 1, 2015 to May 10, 2016 were enrolled. On the day of the surgery, the parents of the children were preoperatively asked to fill out a questionnaire regarding the baseline characteristics and medical history of the children, including gender, age, height, weight, history of URI within the last 2 weeks before anesthesia, history of premature, long-term passive smoking exposure, habitual sleep snoring, and history of asthma. In addition, all adverse respiratory events throughout the perioperative periods (oxygen desaturation, cough, copious secretions, laryngospasm and bronchospasm) as well as peri-operative variables (number of attempts to insert the LMA successfully, anesthesia duration and so on) were recorded. Multivariate Logistic regression analysis was applied to identify independent risk factors of perioperative respiratory adverse events. RESULTS: Among the 232 children included in the study, 28.0% (65/232) presented with a history of a recent URI within the last 2 weeks before anesthesia. The presence of the recent URI increased the incidence of oxygen desaturation (23.1% vs.12.0%, P=0.034), copious secretions (15.4% vs. 6.6%, P=0.036) and any of all the adverse respiratory events (32.3% vs. 18.6%, P=0.024). Multivariate Logistic regression analysis identified two independent risk factors of perioperative adverse respiratory events: a history of URI within the last 2 weeks before general anesthesia (OR=2.021, 95%CI: 1.023-3.994, P=0.043) and habitual sleep snoring (OR=3.660, 95%CI: 1.517-8.832, P=0.004). CONCLUSION: A history of a recent URI within 2 weeks before general anesthesia was associated with a higher incidence of oxygen desaturation, copious secretions and the overall respiratory adverse events. For the children with recent URI, we recommend the general anesthesia and elective surgery should be postponed for at least 2 weeks after the URI.


Asunto(s)
Anestesia General , Máscaras Laríngeas , Infecciones del Sistema Respiratorio , Anestesia General/efectos adversos , Niño , China , Procedimientos Quirúrgicos Electivos , Humanos , Incidencia , Estudios Prospectivos
6.
Clin Exp Obstet Gynecol ; 43(5): 678-684, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-30074318

RESUMEN

The aim of the study was to review patients characteristics, describe the exact anatomic locations and size of the endometriosis in the abdominal wall, and discuss the factors that may contribute to mesh use during abdominal wall endometriosis (AWE) resection. MATERIALS AND METHODS: Patients diagnosed with AWE in their surgical scars from January 2008 to December 2014 were documented. Descriptive data was collected and analyzed. RESULTS: A total of 95 patients with an age ranging from 26 to 48 years, with a mean age of 33.5 ?5.0 years at the time of excision were analyzed. The mean diameter of the mass was 3.25 cm in the present series with an average of 4.97 cm in the mesh group by ultrasound. A total of 18 patients had mesh therapy for fascia defect compared with 77 non-mesh therapy patients. The size of the lesions, the mean duration of symptoms for painful mass, and level of the serum CA125 were statistically different between mesh group and non-mesh group (p < 0.05). Cases of endometriosis lesions limited to the adipose layer had significant lower chance of using mesh (p < 0.05). However, adipose layer endometriosis lesions that had penetrated through the fascia layer and invaded into rectus abdominis muscle layer with/without peritoneum layer had significant higher chance of using mesh (p < 0.05). CONCLUSIONS: The more common position for scar endometriosis may be in the adipose layer at the corner of the surgical scar. Mesh therapy should be considered before surgery when the diameter of the abdominal wall mass detected by ultrasound is more than five em and/or when the lesions invade into rectus abdominis muscle with/without peritoneum tissues from adipose and fascia layers.


Asunto(s)
Pared Abdominal/patología , Cesárea/efectos adversos , Endometriosis/etiología , Complicaciones Posoperatorias/etiología , Adulto , Antígeno Ca-125/sangre , Preescolar , Cicatriz/patología , Endometriosis/diagnóstico por imagen , Femenino , Humanos , Proteínas de la Membrana/sangre , Persona de Mediana Edad , Embarazo , Recto del Abdomen/patología , Ultrasonografía
7.
Genet Mol Res ; 14(3): 8229-35, 2015 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-26345748

RESUMEN

Hemophilia A (HA) is an inherited X-linked bleeding disorder caused by mutations in the factor VIII gene. Prenatal detection in female carriers from families with HA is important to reduce the number of HA patients. The purpose of this study was to detect carriers in families with HA from Sichuan, China, using linkage analysis and a direct genotyping method. A total of 18 HA families were studied. Using a combination of intron 22 inversion, intron 1 inversion, the BclI polymorphic site in intron 18, the HindIII polymorphic site in intron 19, and dinucleotide CA-repeat markers in introns 1, 13, 22, and 24, we were able to detect HA in 88.9% (16/18) of the families studied. HA was detected in the remaining two families by direct genotyping. This study gave the participants a good understanding of their genetic condition and gave us a preliminary understanding of the prevalence of each mutation in Sichuan HA patients.


Asunto(s)
Factor VIII/genética , Tamización de Portadores Genéticos , Ligamiento Genético , Hemofilia A/genética , Adulto , China , Inversión Cromosómica/genética , Femenino , Genotipo , Heterocigoto , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Intrones/genética , Masculino , Mutación
8.
Genet Mol Res ; 14(3): 8716-24, 2015 Jul 31.
Artículo en Inglés | MEDLINE | ID: mdl-26345803

RESUMEN

The purpose of this study was to examine the changes of cellular cholesterol efflux from macrophages in patients with type II diabetes mellitus (DM), and to determine the expression of CYP7A1, ABCG5, and LXRß therein. We recruited 30 patients with type II DM (including 15 patients complicated with coronary heart disease and 15 patients with DM only) and 15 normal controls for this study. Peripheral blood monocytes were isolated for macrophage culture. The mRNA and protein expression levels of CYP7A1, ABCG5, and LXRß were determined using real-time polymerase chain reaction and western blot. The macrophage cholesterol efflux rate was determined with 10% autoserum and standard serum as receptors. We determined that the expression levels of macrophage CYP7A1 mRNA and protein in the type II DM group were significantly lower than those in the control group, but no differences were found in the ABCG5 and LXRß expression levels between the groups. The macrophage cholesterol efflux rate in the patients with type II DM was also significantly decreased compared with that of the normal control subjects (P < 0.01). Furthermore, CYP7A1 mRNA expression and macrophage cholesterol efflux rate were significantly positively correlated. In summary, this study demonstrated that the macrophage cholesterol efflux in patients with type II DM was significantly reduced, and that this reduction was associated with the down-regulation of CYP7A1 expression.


Asunto(s)
Colesterol 7-alfa-Hidroxilasa/genética , Colesterol/metabolismo , Enfermedad Coronaria/enzimología , Diabetes Mellitus Tipo 2/enzimología , Macrófagos/enzimología , Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 5 , Transportadoras de Casetes de Unión a ATP/genética , Transportadoras de Casetes de Unión a ATP/metabolismo , Adulto , Estudios de Casos y Controles , Células Cultivadas , Colesterol/sangre , Colesterol 7-alfa-Hidroxilasa/metabolismo , Enfermedad Coronaria/sangre , Enfermedad Coronaria/etiología , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/complicaciones , Regulación hacia Abajo , Represión Enzimática , Femenino , Humanos , Lipoproteínas/genética , Lipoproteínas/metabolismo , Receptores X del Hígado , Macrófagos/metabolismo , Masculino , Persona de Mediana Edad , Receptores Nucleares Huérfanos/genética , Receptores Nucleares Huérfanos/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo
9.
Genet Mol Res ; 14(1): 860-70, 2015 Feb 02.
Artículo en Inglés | MEDLINE | ID: mdl-25730025

RESUMEN

Cohn fraction IV (CFIV) is a byproduct of a plasma fractionation process known as the Cohn process. It is an inexpensive source of protein C, retaining about 90% of protein C (PC) in human plasma. We investigated whether PC is affected during the Cohn process and evaluated correlations among coagulant activity, amidolytic activity and PC antigen during the Cohn process. CFIV was redissolved with citrate-buffered saline for 5 h at 4°C, and then centrifuged at 3500 g for 40 min at 4°C. Functional anticoagulant activity was measured with a one-stage coagulation method based on activated partial thromboplastin time. The functional amidolytic activity of PC was determined using chromogenic substrate assay, and measurement of PC antigen was performed by ELISA. In CFIV, anticoagulant activity declined significantly, with a loss of >80%, while amidolytic activity was not significantly altered, compared to PC antigen. Prior to the Cohn process, high-rank correlations were observed in cryosupernatant, with rs = 0.921 for anticoagulant and amidolytic activities (P = 0.009), 0.896 for anticoagulant activity and antigen (P = 0.014) and 0.832 for amidolytic activity and antigen (P = 0.031). After the Cohn process in CFIV, there was also a high correlation between amidolytic activity and antigen (rs = 0.782, P = 0.038). There were no significant correlations between anticoagulant activity and antigen (rs = 0.223, P = 0.653), or anticoagulant and amidolytic activity (rs = 0.236, P = 0.675). We conclude that the Cohn process significantly influences the anticoagulant activity of PC. Compared to the antigen, PC lost greater than 80% of its anticoagulant activity, but retained its amidolytic activity, during the Cohn process.


Asunto(s)
Anticoagulantes/sangre , Coagulación Sanguínea/genética , Proteínas Sanguíneas/metabolismo , Proteína C/metabolismo , Antígenos/sangre , Proteínas Sanguíneas/genética , Ensayo de Inmunoadsorción Enzimática , Humanos , Proteína C/genética
10.
Genet Mol Res ; 14(4): 16431-7, 2015 Dec 09.
Artículo en Inglés | MEDLINE | ID: mdl-26662440

RESUMEN

Like other developing countries, China was reported to have a relatively high seroprevalence of anti-hepatitis A antibodies (anti-HAV). However, no studies have evaluated the prevalence of anti-HAV and HAV RNA among voluntary blood donors with or without elevated serum alanine transaminase (ALT) levels. Anti-HAV antibodies were detected using an enzyme-linked immunosorbent assay, and reverse transcription quantitative polymerase chain reaction was carried out for detection of HAV RNA. In the current study, we analyzed a total of 450 serum samples with elevated ALT levels (≥40 U/L) and 278 serum samples with non-elevated ALT levels. Seroprevalence rates of anti-HAV were 51.6% in donors with elevated ALT and 41.4% in donors with non-elevated ALT; however, none of the samples was positive for HAV RNA. The results of our study showed lower seroprevalence rates of anti-HAV in blood donors (irrespective of ALT levels) than those in published data on Chinese populations. Although donors with elevated ALT had statistically higher prevalence rates of anti- HAV than did those with non-elevated ALT, none of the serum samples had detectable levels of the active virus. In conclusion, our results demonstrate that the transmission of hepatitis A by blood transfusion will occur rarely.


Asunto(s)
Virus de la Hepatitis A Humana/inmunología , Hepatitis A/epidemiología , Hepatitis A/inmunología , Adolescente , Adulto , China/epidemiología , Femenino , Hepatitis A/virología , Anticuerpos de Hepatitis A/sangre , Humanos , Pruebas de Función Hepática , Masculino , Persona de Mediana Edad , Estudios Seroepidemiológicos , Carga Viral , Adulto Joven
11.
Genet Mol Res ; 13(3): 6848-54, 2014 Aug 29.
Artículo en Inglés | MEDLINE | ID: mdl-25177964

RESUMEN

This study aimed to investigate the value of magnetic resonance spectroscopy (MRS) imaging in assessing nasopharyngeal carcinoma radiotherapy during the early delayed reaction period. Eighty cases of nasopharyngeal cancer treated with radiotherapy within the same period underwent MRS imaging before or after radiotherapy. Of the 80 cases, 47 underwent MRS imaging on the 3rd, 4th, 6th, and 12th months after radiotherapy. The trends of the primary metabolite concentration at different time points were monitored and compared with the corresponding data after radiotherapy. Repeated measures analysis of variance was performed. At the end of radiotherapy, the N-acetyl aspartate (NAA)/creatine (Cr), choline (Cho)/Cr, and NAA/Cho ratios were reduced to the lowest levels after 3 months. However, increasing trends were observed from the 4th to the 12th month. On the 12th month, stable levels were reached with statistically significant differences (F = 316.02, 53.84, 286.68; P < 0.01). MRS reflected the radiation injury-repair process in the brain of a nasopharyngeal cancer patient during early delayed reaction. This non-invasive monitoring of changes in brain tissue metabolite concentrations provides valuable information for prognosis.


Asunto(s)
Lesiones Encefálicas/diagnóstico , Imagen por Resonancia Magnética/métodos , Espectroscopía de Resonancia Magnética/métodos , Neoplasias Nasofaríngeas/radioterapia , Traumatismos por Radiación/diagnóstico , Adulto , Ácido Aspártico/análogos & derivados , Ácido Aspártico/metabolismo , Lesiones Encefálicas/etiología , Lesiones Encefálicas/metabolismo , Colina/metabolismo , Creatina/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pronóstico , Traumatismos por Radiación/etiología , Traumatismos por Radiación/metabolismo , Radioterapia/efectos adversos , Radioterapia/métodos , Factores de Tiempo
12.
Brain Behav ; 14(6): e3583, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38841826

RESUMEN

OBJECTIVE: To investigate the prevalence of neuroimaging in patients with primary headaches and the clinician-based rationale for requesting neuroimaging in China. DATA SOURCES AND STUDY SETTING: This study included patients with primary headaches admitted to hospitals and clinicians in China. We identified whether neuroimaging was requested and the types of neuroimaging conducted. STUDY DESIGN: This was a cross-sectional study, and convenience sampling was used to recruit patients with primary headaches. Clinicians were interviewed using a combination of personal in-depth and topic-selection group interviews to explore why doctors requested neuroimaging. DATA COLLECTION: We searched for the diagnosis of primary headache in the outpatient and inpatient systems according to the International Classification of Diseases-10 code of patients admitted to six hospitals in three provincial capitals by 2022.We selected three public and three private hospitals with neurology specialties that treated a corresponding number of patients. PRINCIPLE FINDINGS: Among the 2263 patients recruited for this study, 1942 (89.75%) underwent neuroimaging. Of the patients, 1157 (51.13%) underwent magnetic resonance imaging (MRI), 246 (10.87%) underwent both head computed tomography (CT) and MRI, and 628 (27.75%) underwent CT. Fifteen of the 16 interviewed clinicians did not issue a neuroimaging request for patients with primary headaches. Furthermore, we found that doctors issued a neuroimaging request for patients with primary headaches mostly, to exclude the risk of misdiagnosis, reduce uncertainty, avoid medical disputes, meet patients' medical needs, and complete hospital assessment indicators. CONCLUSIONS: For primary headaches, the probability of clinicians requesting neuroimaging was higher in China than in other countries. There is considerable room for improvement in determining appropriate strategies to reduce the use of low-value care for doctors and patients.


Asunto(s)
Imagen por Resonancia Magnética , Neuroimagen , Humanos , China , Estudios Transversales , Neuroimagen/métodos , Neuroimagen/estadística & datos numéricos , Masculino , Adulto , Femenino , Persona de Mediana Edad , Cefaleas Primarias/diagnóstico por imagen , Tomografía Computarizada por Rayos X/estadística & datos numéricos , Adulto Joven , Cefalea/diagnóstico por imagen , Adolescente
13.
Actas Urol Esp (Engl Ed) ; 48(4): 295-303, 2024 May.
Artículo en Inglés, Español | MEDLINE | ID: mdl-38160794

RESUMEN

OBJECTIVE: Tislelizumab, a monoclonal antibody against programed death protein-1 (PD-1), has shown encouraging antitumor activity in urothelial cancer. This study was designed to assess the efficacy and safety of tislelizumab in urotelial cancer in a real-world setting. METHODS: The study was a real-world retrospective study undertaken at Liaoning Cancer Hospital & Institute, China. Eligible patients were ≥18 years. Patients received 200-mg tislelizumab monotherapy intravenously every 3 weeks until the disease progressed to intolerable toxicity. Outcomes included an objective response rate (ORR), disease control rate (DCR), progression-free survival (PFS), overall survival (OS) and safety. RESULTS: Between March 2020 and December 2022, 33 patients were enrolled. The median follow-up was 10.17 (IQR 5.73-12.47) months. Of all 33 patients, ORR and DCR were 30.30% (95% CI 15.6%-48.7%) and 42.42% (95% CI 25.48%-60.78%), respectively. The median PFS was 5.73 (95% CI 3.27-13.00) months, with a 12-month PFS rate of 31.90% (95% CI 19.20%-53.00%). The median OS was 17.7 (95% CI 12.80-not reach) months, with a 12-month OS rate of 67.50% (95% CI 52.70%-86.40%). Eleven (33.33%) and 8 (24.24%) experienced ≥grade 3 treatment-related adverse events (TRAEs) and immune-related Aes, respectively. No treatment-related deaths occurred. CONCLUSION: The excellent efficacy and controllable safety of tislelizumab in locally advanced or metastatic urothelial cancer suggest that it may be a promising therapeutic option for this population.


Asunto(s)
Anticuerpos Monoclonales Humanizados , Carcinoma de Células Transicionales , Humanos , Anticuerpos Monoclonales Humanizados/uso terapéutico , Masculino , Femenino , Estudios Retrospectivos , Persona de Mediana Edad , Anciano , Carcinoma de Células Transicionales/tratamiento farmacológico , Receptor de Muerte Celular Programada 1/antagonistas & inhibidores , Resultado del Tratamiento , Neoplasias Urológicas/tratamiento farmacológico , Neoplasias de la Vejiga Urinaria/tratamiento farmacológico , Anciano de 80 o más Años
14.
Endoscopy ; 45(2): 79-85, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23364839

RESUMEN

BACKGROUND AND STUDY AIMS: In vivo molecular imaging represents a powerful tool for the immediate diagnosis of gastric cancer. In this study, the monoclonal antibody MG7, which is a specific molecular marker against gastric cancer, was labeled with fluorescent agents to enable in vivo real-time imaging by confocal laser endomicroscopy (CLE). PATIENTS AND METHODS: In vivo molecular imaging was performed in tumor-bearing mice from two kinds of human gastric cancer cell lines. Xenograft tumors were visualized in vivo first with a whole-body fluorescent imaging device and then by CLE using fluorescently labeled MG7 antibody. Gastric cancerous tissue and noncancerous mucosa from human biopsies or surgical specimens were also examined ex vivo by CLE. RESULTS: Intravital imaging of xenograft tumors revealed a specific cellular signal, whereas no specific signal was observed in control tissue or in mice injected with irrelevant antibodies. An ex vivo experiment on human specimens using a rigid confocal probe showed positive fluorescent staining in 22/23 samples diagnosed as gastric cancer and weak signals in 5/23 noncancerous tissue samples. CLE evaluation correlated well with immunohistochemical findings. CONCLUSIONS: Screening tumors in vivo by CLE may help to detect MG7-Ag-positive tissues, decrease the sampling error by screening the large tumor surface not routinely screened by biopsy or conventional immunohistochemistry, and facilitate early detection of gastric carcinoma.


Asunto(s)
Anticuerpos Monoclonales , Antígenos de Neoplasias/inmunología , Carcinoma/diagnóstico , Imagen Molecular , Neoplasias Gástricas/diagnóstico , Adulto , Anciano , Anciano de 80 o más Años , Animales , Anticuerpos Monoclonales/inmunología , Carcinoma/inmunología , Carcinoma/patología , Línea Celular Tumoral , Femenino , Humanos , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos BALB C , Microscopía Confocal , Microscopía Fluorescente , Persona de Mediana Edad , Método Simple Ciego , Neoplasias Gástricas/inmunología , Neoplasias Gástricas/patología
15.
Eur Rev Med Pharmacol Sci ; 17(22): 3039-45, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24302184

RESUMEN

AIM: To evaluate the value of Lugol's iodine solution staining combined with endoscope on the diagnosis of non-erosive reflux disease (NERD). PATIENTS AND METHODS: A total of 96 gastroesophageal reflux disease patients were selected to participate in this study. The patients were stained on esophageal mucosa by Lugol's iodine solution and examined at routine endoscopy. The shallow staining and/or non-staining group patients were treated with esomeprazole and mosapride citrate, and then the changes in Lugol's iodine staining, Gerd Q (Gerd questionnaire) scoring and histological characters of esophageal mucosa were recorded before and after treatment. RESULTS: As the results, a total of 68 patients were diagnosed as NERD, and 36 of 68 patients were observed with uniform staining and 32 of 68 patients were observed with shallow staining and/or non-staining. After 4 weeks for treatment, 28 of 32 patients with shallow staining and/or non-staining became uniform staining and 4 of 32 patients were still with shallow staining and/or non-staining. Before and after treatment, the Gerd Q scoring of uniform staining groups and shallow staining and/or non-staining groups all had a significant difference (p < 0.05). Compared to the routine endoscopy, the detection rate of abnormal esophageal mucosa was significantly increased by Lugol's iodine solution staining combined with endoscope. CONCLUSIONS: Lugol's iodine solution staining combined with routine endoscopy, Gerd Q scoring and histomorphology can be used to evaluate the diagnosis and therapeutic effect of NERD.


Asunto(s)
Endoscopía/métodos , Reflujo Gastroesofágico/diagnóstico , Yoduros , Adulto , Femenino , Reflujo Gastroesofágico/patología , Humanos , Masculino , Persona de Mediana Edad , Coloración y Etiquetado
16.
Genet Mol Res ; 12(4): 6813-24, 2013 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-24391029

RESUMEN

Human coagulation factor VII (FVII) plays an important role in the blood coagulation process and exists in micro amounts in human plasma; therefore, any attempt at the large-scale production of FVII in significant quantities is challenging. The purpose of this study was to express and obtain biologically active recombinant FVII (rFVII) from Chinese hamster ovary K1 (CHO-K1) cells. The full-length FVII cDNA was isolated from a HepG2 cell line and then subcloned in pcDNA3.1 to construct an expression vector, pcDNA-FVII. CHO-K1 cells were transfected with 1 µg pcDNA-FVII. The cell line that stably expressed secretory FVII was screened using 900 µg/mL G418. The FVII copy number in CHO-K1 cells was detected by quantitative polymerase chain reaction (qPCR). The rFVII was purified in ligand affinity chromatography medium. The purified protein was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The biological activity of the purified FVII protein was determined by a prothrombin time assay. Three cell lines that permanently expressed rFVII were screened. The qPCR results demonstrated that each CHO-K1 cell harbored two FVII DNA copies. The SDS-PAGE and Western blot analysis showed that the purified protein was about 50 kDa. The purity of the target protein was 95%. The prothrombin time assay indicated that the FVII-specific activity of rFVII was 2573 ± 75 IU/mg. This method enabled the fast preparation of high-purity rFVII from CHO-K1 cells, and the purified protein had good biological activity.


Asunto(s)
Clonación Molecular , Factor VII/genética , Proteínas Recombinantes/genética , Animales , Secuencia de Bases , Coagulación Sanguínea/genética , Coagulación Sanguínea/fisiología , Células CHO , Línea Celular , Cricetinae , Cricetulus , Factor VII/biosíntesis , Células Hep G2 , Humanos , Reacción en Cadena en Tiempo Real de la Polimerasa , Proteínas Recombinantes/biosíntesis , Análisis de Secuencia de ADN
17.
Genet Mol Res ; 12(3): 2556-61, 2013 Jul 24.
Artículo en Inglés | MEDLINE | ID: mdl-23315876

RESUMEN

Residual activated coagulation factor XI (FXIa) has been suggested to play an important role in thromboembolic events associated with the use of intravenous immunoglobulin (IVIG) lots. This study investigated the predominant plasma proteases in 42 IVIG lots from 4 Chinese manufacturers. In one-stage clotting assays, the procoagulant activities of factors II, VII, IX, X, XI, and XII were quantified. Non-activated partial thromboplastin time and a modified thrombin generation test served as global and FXIa-specific clotting assays, respectively. We found that coagulation factor clotting activities of the 42 IVIG lots were below the detection limit of the assays, except for the products of manufacturer B (lots of 2010), in which 0.030 to 0.032 IU/mL FXI:C were detected. The peak time of thrombin using a thrombin generation test was greater than 35 min, the relevant amount of FXIa was below 0.37 nM, and non-activated partial thromboplastin time was greater than 200 s. Consequently, the 42 IVIG lots showed non-significant procoagulant potential. Further study is required to determine whether a program for FXIa determination in IVIG products should be launched in China.


Asunto(s)
Contaminación de Medicamentos , Factor XI/análisis , Inmunoglobulinas Intravenosas/química , China , Industria Farmacéutica , Humanos
18.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 34(6): 669-672, 2022 Nov 09.
Artículo en Zh | MEDLINE | ID: mdl-36642912

RESUMEN

Hepatic cystic echinococcosis is a chronic parasitic disease caused by the infection with the larvae of Echinococcus granulosus in human or animal liver tissues. As a chronic active infectious disease, tuberculous empyema mainly invades the pleural space and then causes visceral and parietal pleura thickening. It is rare to present comorbidity for hepatic cystic echinococcosis and tuberculous empyema. This case report presents a case of hepatic cystic echinococcosis complicated with tuberculous empyema misdiagnosed as hepatic and pulmonary cystic echinococcosis, aiming to improve clinicians' ability to distinguish this disorder.


Asunto(s)
Equinococosis Hepática , Equinococosis , Echinococcus granulosus , Empiema Tuberculoso , Animales , Humanos , Empiema Tuberculoso/complicaciones , Equinococosis/diagnóstico , Equinococosis Hepática/complicaciones , Equinococosis Hepática/diagnóstico , Errores Diagnósticos
19.
Biochem Biophys Res Commun ; 407(2): 327-32, 2011 Apr 08.
Artículo en Inglés | MEDLINE | ID: mdl-21382343

RESUMEN

The aim of this study was to construct tissue-engineered composite intervertebral disc (IVD) consisting of demineralized bone matrix gelatin (DBMG) and collagen II/hyaluronate/chondroitin-6-sulfate (CII/HyA-CS) scaffolds seeded with anulus fibrosus (AF) and nucleus pulposus (NP) cells, respectively. The cell-scaffold hybrids were implanted in the subcutaneous space of the dorsum of athymic mice and harvested at 4, 8, and 12 weeks. At each time point, the gross and histological morphology and biochemical properties were evaluated. Our results are as following: the gross morphology and histology of the composite resembled those of native IVD. Morphological studies revealed progressive tissue formation and junction integration between AF and NP regions. Biochemical composition detection indicated that the content of DNA, proteoglycan and hydroxyproline increased with time, and were similar to native tissue at 12 weeks. All these results demonstrated the feasibility of creating a tissue-engineered composite IVD with similar morphological and biochemical properties to the native tissue.


Asunto(s)
Disco Intervertebral/química , Disco Intervertebral/citología , Ingeniería de Tejidos , Andamios del Tejido/química , Animales , Sulfatos de Condroitina/química , Colágeno Tipo II/química , ADN/química , Gelatina/química , Ácido Hialurónico/química , Disco Intervertebral/trasplante , Ratones , Ratones Desnudos , Conejos
20.
Endoscopy ; 43(2): 87-93, 2011 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21038291

RESUMEN

BACKGROUND AND STUDY AIM: The real-time identification and removal of adenomas is a cost-effective strategy to improve the prognosis of colorectal cancer. Confocal laser endomicroscopy (CLE) could provide real-time histological-level observation. We aimed to evaluate the efficacy of CLE diagnosis using a simple classification system that differentiates adenomas from non-neoplastic polyps with intravenous fluorescein staining alone. PATIENTS AND METHODS: An endoscope integrated confocal laser microscopy system was used in this study. CLE images of 35 colonic polyps, including 15 hyperplastic polyps and 20 adenomas confirmed by histology, were first evaluated to develop criteria for diagnosis of neoplastic and non-neoplastic polyps. The diagnostic criteria included goblet cell depletion, villous architecture, and microvascular alterations. We then performed a prospective study of colonic polyps found during CLE and classified them according to the established criteria. A total of 115 patients with 115 colonic polyps were included. The real-time CLE diagnosis was compared with that from histology. The stored CLE images were evaluated later by a blinded observer. RESULTS: The sensitivity, specificity, positive predictive value, and negative predictive value of real-time CLE in identifying colonic adenomas were 93.9 % (95 % confidence interval [CI] 85.4 - 97.6), 95.9 % (95 % CI 86.2 - 98.9), 96.9 % (95 % CI 89 - 99), and 92.2 % (95 % CI 81 - 97), respectively, compared with histological results. Interobserver agreement between real-time and post-CLE still-image evaluation was excellent (kappa = 0.929). Goblet cell depletion alone had a sensitivity of 84.9 % (95 % CI 73 - 92) and a specificity of 87.8 % (95 % CI 75 - 95), as well as excellent interobserver agreement (kappa = 0.824). CONCLUSIONS: Endoscope integrated CLE with fluorescein staining may reliably assist in the real-time identification of colonic adenomas. Among three diagnostic categories, goblet cell depletion can be used to distinguish adenomas and hyperplastic polyps.


Asunto(s)
Adenoma/patología , Neoplasias del Colon/patología , Pólipos del Colon/patología , Colonoscopía/métodos , Microscopía Confocal , Lesiones Precancerosas/patología , Adenoma/clasificación , Adulto , Neoplasias del Colon/clasificación , Pólipos del Colon/clasificación , Pólipos del Colon/diagnóstico , Colonoscopía/instrumentación , Femenino , Colorantes Fluorescentes , Células Caliciformes/patología , Humanos , Masculino , Persona de Mediana Edad , Variaciones Dependientes del Observador , Lesiones Precancerosas/clasificación , Lesiones Precancerosas/diagnóstico , Valor Predictivo de las Pruebas , Estudios Prospectivos , Método Simple Ciego
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