Sub-50†fs, 0.5†W average power Nd-doped fiber amplifier at 920†nm.

We develop an all polarization-maintaining (PM) 920 nm Nd-doped fiber amplifier delivering a train of pulses with ∼0.53 W average power and sub-50 fs duration. The sub-50 fs pulse benefits from the pre-chirping management method that allows for over 60 nm broadening spectrum without pulse breaking in the amplification stage. By virtue of the short pulse duration, the pulse peak power can reach to ∼0.31 MW in spite of the moderate average power. These results represent a key step in developing high-peak-power pulse Nd-doped fiber laser systems at 920 nm, which will find important applications in fields such as biomedical imaging, ultrafast optical spectroscopy, and excitation of quantum-dot single photon sources.

Optical focusing inside scattering media with iterative time-reversed ultrasonically encoded near-infrared light.

Focusing light inside scattering media is a long-sought goal in optics. Time-reversed ultrasonically encoded (TRUE) focusing, which combines the advantages of biological transparency of the ultrasound and the high efficiency of digital optical phase conjugation (DOPC) based wavefront shaping, has been proposed to tackle this problem. By invoking repeated acousto-optic interactions, iterative TRUE (iTRUE) focusing can further break the resolution barrier imposed by the acoustic diffraction limit, showing great potential for deep-tissue biomedical applications. However, stringent requirements on system alignment prohibit the practical use of iTRUE focusing, especially for biomedical applications at the near-infrared spectral window. In this work, we fill this blank by developing an alignment protocol that is suitable for iTRUE focusing with a near-infrared light source. This protocol mainly contains three steps, including rough alignment with manual adjustment, fine-tuning with a high-precision motorized stage, and digital compensation through Zernike polynomials. Using this protocol, an optical focus with a peak-to-background ratio (PBR) of up to 70% of the theoretical value can be achieved. By using a 5-MHz ultrasonic transducer, we demonstrated the first iTRUE focusing using near-infrared light at 1053 nm, enabling the formation of an optical focus inside a scattering medium composed of stacked scattering films and a mirror. Quantitatively, the size of the focus decreased from roughly 1 mm to 160 µm within a few consecutive iterations and a PBR up to 70 was finally achieved. We anticipate that the capability of focusing near-infrared light inside scattering media, along with the reported alignment protocol, can be beneficial to a variety of applications in biomedical optics.

Periodic transition between two evolving soliton pulsation states in an Yb-doped fiber laser.

Due to the fascinating features, pulsating solitons attract much attention in the field of nonlinear soliton dynamics and ultrafast lasers. So far, most of the investigations on pulsating soliton are conducted in Er-doped fiber lasers. In this work, we reported the periodic transition between two evolving pulsating soliton states in an Yb-doped fiber laser. By using the real-time measurement techniques, the spectral and temporal characteristics of this transition state were investigated. Two evolving soliton pulsation states have similar evolution process, i.e., from pulsating towards quasi-stable mode-locked states. However, the details of the two processes are different, such as the pulse energy levels, pulsating modulation depths, duration of quasi-stable mode-locked states. The transition between two evolving soliton pulsation states could be attributed to the interaction of the polarizer and the varying polarization states of the pulse inside the laser cavity. The experimental results will contribute to the further understanding of soliton pulsating dynamics in dissipative optical systems.

Auto-setting multi-soliton temporal spacing in a fiber laser by a hybrid GA-PSO algorithm.

Multi-soliton operation in fiber lasers is a promising platform for the investigation of soliton interaction dynamics and high repetition-rate pulse. However, owing to the complex interaction process, precisely manipulating the temporal spacing of multiple solitons in a fiber laser is still challenging. Herein, we propose an automatic way to control the temporal spacing of multi-soliton operation in an ultrafast fiber laser by a hybrid genetic algorithm-particle swarm optimization (GA-PSO) algorithm. Relying on the intelligent adjustment of the electronic polarization controller (EPC), the on-demand temporal spacing of the double solitons can be effectively achieved. In particular, the harmonic mode locking with equal temporal spacing of double solitons is also obtained. Our approach provides a promising way to explore nonlinear soliton dynamics in optical systems and optimize the performance of ultrafast fiber lasers.

Assisting the mode-locking of a figure-9 fiber laser by thermal nonlinearity of graphene-decorated microfiber.

The self-starting performance of a figure-9 fiber laser is critically dependent on the phase shift difference between the counter-propagating beams. Herein, we propose an effective approach to dynamically control the phase shift difference in a figure-9 fiber laser by utilizing the thermal nonlinearity of graphene-decorated microfiber device. With the adjustment of the control laser power injected into the graphene-decorated microfiber, the self-starting mode-locked threshold of the figure-9 fiber laser can be attained in a flexible pump power range, i.e., from 300 mW to 390 mW. These findings demonstrated that the graphene-decorated microfiber could act as a dynamical control device of phase shift difference for improving the performance of figure-9 fiber lasers, and might also open up new possibilities for applications of microfiber photonic devices in the field of ultrafast optics.

Revealing the pulse dynamics in a Mamyshev oscillator: from seed signal to oscillator pulse.

The Mamyshev oscillator (MO) is a promising platform to generate high-peak-power pulse with environmentally stable operation. However, rare efforts have been dedicated to unveil the dynamics from seed signal to oscillator pulse, particularly for the multi-pulse operation. Herein, we investigate the buildup dynamics of the oscillator pulse from the seed signal in a fiber MO. It is revealed that the gain competition among the successively injected seed pulses leads to higher pump power that is required to ignite the MO, hence resulting in the higher optical gain that supports buildup of multiple oscillator pulses. The multiple oscillator pulses are identified to be evolved from the multiple seed pulses. Moreover, the dispersive Fourier transform (DFT) technique is used to reveals the real-time spectral dynamics during the starting process. As a proof-of-concept demonstration, a highly intensity-modulated pulse bunch was employed as the seed signal to reduce the gain competition effect and avoid the multi-pulse starting operation. The experimental results are verified by numerical simulations. These findings would give new insights into the pulse dynamics in MO, which will be meaningful to the communities interested in ultrafast laser technologies and nonlinear optics.

Antimicrobial resistance of Escherichia coli, Enterobacter spp., Klebsiella pneumoniae and Enterococcus spp. isolated from the feces of giant panda.

BACKGROUND: Escherichia coli, Enterobacter spp., Klebsiella pneumoniae and Enterococcus spp., common gut bacteria in giant pandas, include opportunistic pathogens. The giant panda is an endangered species, classified as vulnerable by the World Wildlife Foundation. Continuous monitoring for the emergence of antimicrobial resistance (AMR) among bacterial isolates from giant pandas is vital not only for their protection but also for public health. RESULTS: A total of 166 E. coli, 68 Enterobacter spp., 116 K. pneumoniae and 117 Enterococcus spp. isolates were collected from fecal samples of 166 giant pandas. In the antimicrobial susceptibility tests, 144 E. coli isolates, 66 Enterobacter spp. isolates, 110 K. pneumoniae isolates and 43 Enterococcus spp. isolates were resistant to at least one antimicrobial. The resistant isolates carried antimicrobial resistance genes (ARGs), including sul3, blaTEM, blaSHV and tetA. The differences in the prevalence of the bla types implied that the genetic basis for ß-lactam resistance among the E. coli, Enterobacter spp. and K. pneumoniae isolates was different. The strain K. pneumoniae K85 that was resistant to sixteen antimicrobials was selected for whole genome sequencing. The genome contained Col440I, IncFIBK and IncFIIK plasmids and altogether 258 ARGs were predicted in the genome; 179 of the predicted ARGs were efflux pump genes. The genetic environment of the ß-lactamase genes blaCTX-M-3 and blaTEM-1 in the K. pneumoniae K85 genome was relatively similar to those in other sequenced K. pneumoniae genomes. In comparing the giant panda age groups, the differences in the resistance rates among E. coli, K. pneumoniae and Enterobacter spp. isolates suggested that the infections in giant pandas of different age should be treated differently. CONCLUSIONS: Antimicrobial resistance was prevalent in the bacterial isolates from the giant pandas, implying that the gut bacteria may pose serious health risks for captive giant pandas. The resistance genes in the genome of K. pneumoniae K85 were associated with insertion sequences and integron-integrase genes, implying a potential for the further spread of the antimicrobial resistance.

1.7 µm figure-9 Tm-doped ultrafast fiber laser.

The evolution of multiphoton microscopy is critically dependent on the development of ultrafast laser technologies. The ultrashort pulse laser source at 1.7 µm waveband is attractive for in-depth three-photon imaging owing to the reduced scattering and absorption effects in biological tissues. Herein, we report on a 1.7 µm passively mode-locked figure-9 Tm-doped fiber laser. The nonreciprocal phase shifter that consists of two quarter-wave plates and a Faraday rotator introduces phase bias between the counter-propagating beams in the nonlinear amplifying loop mirror. The cavity dispersion is compensated to be slightly positive, enabling the proposed 1.7 µm ultrafast fiber laser to deliver the dissipative soliton with a 3-dB bandwidth of 20 nm. Moreover, the mode-locked spectral bandwidth could be flexibly tuned with different phase biases by rotating the wave plates. The demonstration of figure-9 Tm-doped ultrafast fiber laser would pave the way to develop the robust 1.7 µm ultrashort pulse laser sources, which could find important application for three-photon deep-tissue imaging.

Metagenomic analysis revealed a wide distribution of antibiotic resistance genes and biosynthesis of antibiotics in the gut of giant pandas.

BACKGROUND: The gut microbiome is essential for the host's health and serves as an essential reservoir of antibiotic resistance genes (ARGs). We investigated the effects of different factors, including the dietary shifts and age, on the functional characteristics of the giant panda's gut microbiome (GPs) through shotgun metagenome sequencing. We explored the association between gut bacterial genera and ARGs within the gut based on network analysis. RESULTS: Fecal samples (n=60) from captive juvenile, adult, and geriatric GPs were processed, and variations were identified in the gut microbiome according to different ages, the abundance of novel ARGs and the biosynthesis of antibiotics. Among 667 ARGs identified, nine from the top ten ARGs had a higher abundance in juveniles. For 102 ARGs against bacteria, a co-occurrence pattern revealed a positive association for predominant ARGs with Streptococcus. A comparative KEGG pathways analysis revealed an abundant biosynthesis of antibiotics among three different groups of GPs, where it was more significantly observed in the juvenile group. A co-occurrence pattern further revealed a positive association for the top ten ARGs, biosynthesis of antibiotics, and metabolic pathways. CONCLUSION: Gut of GPs serve as a reservoir for novel ARGs and biosynthesis of antibiotics. Dietary changes and age may influence the gut microbiome's functional characteristics; however, it needs further studies to ascertain the study outcomes.

Autosetting soliton pulsation in a fiber laser by an improved depth-first search algorithm.

Soliton pulsation is one of the most fascinating phenomena in ultrafast fiber lasers, owing to its rich nonlinear dynamics and potential generation of high peak power pulse. However, it is still a challenge to efficiently search for pulsating soliton in fiber lasers because it requires a fine setting of laser cavity parameters. Here, we report the autosetting soliton pulsation in a passively mode-locked fiber laser. The parameters of electronic polarization controller are intelligently adjusted to search for pulsating soliton state by the improved depth-first search algorithm. Moreover, the intensity modulation depth of pulsating soliton could be flexibly controlled. These findings indicate that the intelligent control of a fiber laser is an effective way to explore on-demand soliton dynamics and is also beneficial to the optimization of ultrafast laser performance.

Mutually induced soliton polarization instability in a bidirectional ultrafast fiber laser.

The bidirectional ultrafast fiber laser is a promising light source for dual-comb applications. The counter-propagating geometry could lead to soliton interaction through gain sharing, as well as the possible outcome of polarization instability. However, the polarization dynamics hidden behind the soliton interaction process in bidirectional fiber lasers were rarely investigated. Herein, we report on the polarization instability induced by the mutual soliton interactions through fiber gain in a bidirectional mode-locked fiber laser. Depending on the adjustment of the intracavity birefringence, the polarization states of two counter-propagating solitons can exhibit similar periodical polarization switching behaviors with a polarization-rotating transition state. The successive interactions of the bidirectional solitons mediated by the polarization cross-saturation effect of gain fiber could be responsible for the soliton polarization instability. These findings, in addition to the fundamental interest of the soliton nonlinear dynamics in dissipative optical systems, also open up new possibilities for creating dynamical control of the soliton polarization state and performance improvement in bidirectional ultrafast fiber lasers.

The Fecal and Serum Metabolomics of Giant Pandas Based on Untargeted Metabolomics.

Little is comprehensively known or understood about giant panda fecal and serum metabolites, which could serve as important indicators of the physiological metabolism of giant pandas. Therefore, we determined the contents of fecal and serum metabolites of giant pandas based on an untargeted metabolome. Four hundred and 955 metabolites were detected in the feces and serum of giant panda, respectively. Glycerophospholipid and choline metabolism were the main metabolic pathways in feces and serum. A significant correlation between the gut microbiota and fecal metabolites was found (P < 0.01). Fecal metabolites were not greatly affected by the age or gender of giant pandas, but serum metabolites were significantly affected by age and gender. The majority of different metabolites caused by age were higher in serum of younger giant pandas, including fatty acids, lipids, metabolites of bile acids, and intermediate products of vitamin D3. The majority of different metabolites caused by gender included fatty acids, phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylethanolamine (PE). A separate feeding diet should be considered according to different ages and genders of giant panda. Therefore, our results could provide helpful suggestions to further protect captive giant pandas.

Diversity and Composition of Gut Bacterial Community in Giant Panda with Anorexia.

The giant panda (GP) is the most precious animal in China. Gastrointestinal tract disease, especially associated with dysbiosis of gut microbiota, is the leading cause of death in GPs. Here, we performed 16S rRNA high-throughput sequencing to investigate the gut microbiota of GPs having symptoms of anorexia. Results showed that gut microbiota of GP with anorexia had lower richness (Chao1 index) than the healthy GP. However, no significant differences in alpha diversity were observed. There is a significance in the microbial structure between anorexia and healthy GPs. The abundance of phylum Firmicutes (99.23% ± 7.1%), unidentified genus Clostridiales (24.75% ± 2.5%), was significantly higher in the subadult anorexia group (P < 0.01), and that of the unidentified genus Clostridiales (4.53% ± 1.2%) was also significantly higher in the adult anorexia group (P < 0.01). Weissella and Streptococcus were found to be decreased in both anorexia groups. The decreased abundance of Weissella (0.02% ± 0.0%, 0.08% ± 0.0%) and Streptococcus (73.89% ± 4.3%, 91.15% ± 7.6%) and increase in Clostridium may cause symptoms of anorexia in giant pandas. The correlation analysis indicated that there is a symbiotic relationship among Streptococcus, Leuconostoc, Weissella, and Bacillus which are classified as probiotics (r > 0.6, P < 0.05). Importantly, a negative correlation has been found between Streptococcus and unidentified_Clostridium in two groups (r > 0.6, P < 0.05). Our results suggested that Streptococcus might be used as probiotics to control the growth of Clostridium causing the anorexia.

Effects of proanthocyanidins on the pasting, rheological and retrogradation properties of potato starch.

BACKGROUND: Proanthocyanidins (PAS) were complexed with potato starch (PS) to prepare polyphenol-starch complexes. The pasting, rheological and retrogradation properties of the complexes were investigated. RESULTS: The addition of PAS markedly affected the pasting, rheological and retrogradation properties of PS, especially at a concentration of 5% (w/w). Rapid viscosity analysis indicated that PAS significantly changed the viscosity, breakdown and setback value of PS. The rheological results showed that PAS decreased the flow behavior index and consistency coefficient, but increased the viscoelasticity of PS. Differential scanning calorimetry and X-ray diffraction indicated that PAS delayed the retrogradation of PS. Furthermore, scanning electron microscopy indicated that the morphologies of retrograded PS gels were greatly altered to a less compact structure with the presence of PAS. Moreover, Fourier transform infrared spectroscopy elucidated that PAS interacted with PS via a noncovalent interaction, and inhibited the retrogradation of PS. CONCLUSIONS: The findings suggested that supplementing PS with PAS might be an effective and convenient method for modifying the physicochemical properties of PS. © 2021 Society of Chemical Industry.

Vector features of pulsating soliton in an ultrafast fiber laser.

Pulsating soliton in ultrafast fiber lasers has interesting non-stationary dynamics, which is one of the hot topics in field of nonlinear soliton. So far, most researchers only focused on the spectral and temporal characteristics of pulsating soliton. However, the vector features of pulsating soliton were rarely studied. In this work, we experimentally studied the pulsating vector solitons in an ultrafast fiber laser. Three categories of vector solitons with different polarization evolution characteristics could be obtained by adjusting the pump power and polarization controller, such as pulsating polarization-locked vector soliton (PLVS), pulsating polarization-rotation vector soliton (PRVS) and progressive pulsating PRVS. Interestingly, besides the basic polarization rotation with a period of 2 roundtrips, the polarization angle also evolves with progressive mode in the progressive pulsating PRVS state. The abundant results of pulsating vector solitons demonstrate that investigating vector features of nonlinear soliton dynamics is necessary and significant and would greatly enrich the research of soliton dynamics.

Buildup dynamics in an all-polarization-maintaining Yb-doped fiber laser mode-locked by nonlinear polarization evolution.

Soliton buildup dynamics in ultrafast fiber lasers are one of the most significant topics in both the fundamental and industrial fields. In this work, by using the dispersive Fourier transformation technique, the real-time spectral evolution of soliton buildup dynamics were investigated in the all-polarization-maintaining Yb-doped fiber laser, which is mode-locked by nonlinear polarization evolution technique through the cross splicing method. It was experimentally confirmed that the same stable soliton state could be achieved through different soliton starting processes because of the initial random noises. In one case, the maximum pulse energy during the soliton starting process could reach ∼15 times the stable pulse energy, which results in the spectral chaotic state and temporal shift. We also provide another soliton buildup case with the same cavity parameters, which illustrates more moderate evolution. It involves smaller energy variation and no complex transition state. These results would deepen our understanding of soliton buildup dynamics and be beneficial for the applications of ultrafast fiber lasers.

Selective versus non-selective removal for dental caries: a systematic review and meta-analysis.

OBJECTIVE: Selective and non-selective methods for caries removal were controversial so far, thus we aimed to compare the efficacy of selective and non-selective caries removal by conducting meta-analysis of randomized controlled trials (RCTs). MATERIALS AND METHODS: Eligible RCTs studies comparing selective caries removal with non-selective caries removal were retrieved by searching PubMed, EMBASE and Cochrane Library till 15 July 2017. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated for outcome indictors, including pulpal exposure, pulpal symptoms and failure using Inverse variance-random effects or Mantel-Haenszel-fixed effects models. RESULTS: Totally, seven studies were eligible for the meta-analysis. Compared with the non-selective caries removal group, the risk of pulpal exposure was significantly reduced in the selective caries removal group (OR = 0.11, 95% CI: 0.04-0.30). No significant difference was observed in pulpal symptoms (OR = 0.79, 95% CI: 0.30-2.12) and failure (OR = 1.40, 95% CI: 0.69-2.84) between the groups. CONCLUSIONS: The efficacy of selective caries removal appears comparable to that of non-selective caries removal in children, with similar pulpal symptoms and failure, but selective caries removal may result in a low incidence of pulpal exposure. However, larger-scale RCTs with long-term follow-up are required to confirm this conclusion.

Comparison of bioactivities and phenolic composition of Choerospondias axillaris peels and fleshes.

BACKGROUND: Choerospondias axillaris is both an edible and medicinal fruit. It has a growing popularity and economic importance due to its nutritive value and medicinal effects, but comprehensive information on the chemical composition and bioactivity of its fruits is still lacking. Therefore the aim of this study was to investigate the antioxidant, antimicrobial and antiproliferative effects and chemical composition of peel polyphenolic (PP) and flesh polyphenolic (FP) extracts from C. axillaris. RESULTS: The phenolics and flavonoids of peel were significantly higher than those of flesh. Ultra-performance liquid chromatography (UPLC) and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight-mass spectrometry (UPLC/ESI-QTOF-MS(2) ) analysis revealed that (+)-catechin and oligomeric procyanidins were the most abundant compounds in PP and FP. Both extracts exhibited strong ferric-reducing antioxidant power, total antioxidant activity and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH(•) )-scavenging ability. PP showed a significantly higher antimicrobial effect against tested strains than that of FP, in a dose-dependent manner. Furthermore, both extracts inhibited the growth of HepG2 and Caco-2 cells in a dose- and time-dependent manner, with IC50 values of 39.31 and 47.49 µg mL(-1) to HepG2 cells and 101.90 and 102.61 µg mL(-1) to Caco-2 cells respectively. CONCLUSION: This is the first detailed report on the chemical composition and bioactivities of C. axillaris fruits. © 2015 Society of Chemical Industry.

Pectin modifications: a review.

In recent years, the interest in studying modification of pectin has increased. A number of hydroxyl and carboxyl groups distributed along the backbone as well as a certain amount of neutral sugars presented as side chains make pectin capable of preparing a broad spectrum of derivatives. By forming pectin derivatives, their properties may be modified and some other new functional properties may be created. This article attempts to review the information about various methods used for pectin modification, including substitution (alkylation, amidation, quaternization, thiolation, sulfation, oxidation, etc.), chain elongation (cross-linking and grafting) and depolymerization (chemical, physical, and enzymatic degradation). Characteristics and applications of some pectin derivatives are also presented. In addition, the safety and regulatory status of pectin and its derivatives were reviewed.

[Determination of Mineral Elements in Choerospondias Axillaris and Its Extractives by ICP-AES].

Nine elements in Choerospondias axillaris flesh, peels, aqueous extractives and gastric digesta were determined by the inductively coupled plasma atomic emission spectrometry (ICP-AES) in the present study. The results showed that the contents of Fe, Ca, Zn, Mn, Al, Mg, Cu, K and P in the flesh were 27.37, 269.88, 1.51, 2.45, 1.95, 195.30, 2.45, 2,970.11, and 133.94 µg · g(-1), respectively. They are lower than that in the peels, about 40.31%, 11.70%, 21.68%, 4.27%, 10.58%, 15.76%, 68.72%, 42.04%, and 22.59%, respectively. For microwave assistant extraction, the release rate of Mn was highest (81.68%), while Fe was lowest (4.42%) in the flesh. The release rate of Zn was the highest (79.00%), while that of A1 was the lowest (4.94%) in the peels. Except Fe, Cu and Zn, the release rates of the other elements in flesh were higher than those in the peels. After gastric digestion, the release rates of nine elements were 3.25%-87.51% in the flesh and 7.11%-50.69% in the peels. The release rates of minerals in the flesh were found to be higher than those in the peels except Fe and Cu. Microwave assistant extraction can more efficiently release Fe, Ca, Mn, Mg and K from the flesh than the gastric digestion do. While gastric digestion had a significant effect on the peels, the release rates of elements, except Zn, were higher than those in microwave assistant extraction. Therefore, the difference of distribution and release of mineral elements between peels and flesh of Choerospondias axillaris was understood, which will provide a positive guide for further study of bioavailability of minerals for human body.