Morphological characterization of antennae and antennal sensilla of Diaphorina citri Kuwayama (Hemiptera: Liviidae) nymphs.

Diaphorina citri Kuwayama is the most economically important citrus pest which is the primary vector of Candidatus Liberibacter spp. causing citrus greening (huanglongbing, HLB) disease. To better understand the developmental and structural changes of antennae and antennal sensilla in D. citri nymphs, we investigated the antennal morphology, structure and sensilla distribution of the five nymphal stages of D. citri using scanning electron microscopy. The antennae of the five different nymphal stages of D. citri were filiform in shape, which consisted of two segments in the first-, second- and third-instar nymphs; three segments in the fourth- and fifth-instar nymphs. The length of their antennae was significantly increased with the increase of the nymphal instar, as well as the total number of antennal sensilla. Ten morphological sensilla types were recorded altogether. They were the long terminal hair (TH1), short terminal hair (TH2), sensilla trichoidea (ST), cavity sensillum 1 (CvS1), cavity sensillum 2 (CvS2), sensilla basiconica 1-3 (SB1-3), sensilla campaniform (SCA) and partitioned sensory organ (PSO). Also, the distribution of antennal sensilla in each nymphal stage of D. citri was asymmetrical. The SBs only occurred on the antennae of the third-, fourth- and fifth-instar nymphs. Only one CvS2 was found in the third- and fifth-instar nymphs, and one SCA in the fourth- and fifth-instar nymphs, respectively. The possible roles of the nymphal antennal sensilla in D. citri were discussed. The results could contribute to a better understanding of the development of the sensory system, and facilitate future studies on the antennal functions in D. citri nymphs.

Combining citizen science and land use data to identify drivers of eutrophication in the Huangpu River system.

In recent years, the massive land use changes and urbanization of Shanghai City have coincided with a growing eutrophication and an overall degradation of Huangpu River, with related risks to the city's drinking water supply and economic development. However, there is only limited information to evaluate the spatial and temporal changes to the Huangpu River and its many tributaries. In the present study, 400 citizen scientists were trained to monitor water quality and environmental conditions on a monthly basis over three years in the lower (high urbanized) Huangpu River catchment. Their data were integrated with high resolution land cover data using GIS techniques to characterize water quality dynamics of the Huangpu River system with respect to main environmental drivers. Environmental driver analysis indicated that up-catchment conditions dominate river dynamics while typical urban impacts (first flush, impermeable land cover…) have only limited influence. According to these results, the city's investments to improve wastewater treatment and mitigate lower river impacts need to be extended throughout the catchment to reduce nutrient concentrations that are near or above thresholds for rivers and streams. The positive impact of in-stream vegetation pointed to the possibilities that local scale ecological remediation activities to reduce runoff could be viable approaches to improve river conditions throughout the catchment.