Are low birth weight, intrauterine growth restriction, and preterm birth associated with enamel developmental defects?

PURPOSE: The purpose of this study was to estimate the association between low birth weight and its 2 components, intrauterine growth restriction (IUGR) and preterm birth, with developmental defects of dental enamel (DDE). METHODS: A retrospective cohort study was conducted on 205 12- to 60-month-olds born in the municipality of São Luís, Maranhão, Brazil. Clinical examination and interviews with the responsible persons were performed, and the medical records were analyzed. The modified DDE index was used for the diagnosis and classification of enamel defects. Poisson regression analysis was performed, and relative risk (RR) ratios (95% confidence interval, CI) were used for the estimation of associations. RESULTS: Preterm birth was not associated with DDE in nonadjusted or adjusted analysis. In the crude models and after adjusting for confounding factors, IUGR (RR=5.19; CI=2.42-11.13) and low birth weight (RR=3.44; CI=1.72-7.37) continued to be risk factors for the occurrence of DDE. CONCLUSIONS: Low birth weight infants present a higher risk of developing enamel defects. This risk seems to be more related to the IUGR component than to the preterm birth component.

Pharmacognostic and acute toxicological evaluation of Scaptotrigona aff. postica propolis extract in pre-clinical assays.

The propolis of Scaptotrigona aff. postica is popularly used in Maranhão State, Brazil, for treating wounds and respiratory illnesses. Nevertheless, little is known about the chemical composition of this propolis and the adverse effects of its use. Hence, this study is a pharmacognostic characterisation of the propolis hydroalcoholic extract (PHE) from S. aff. postica. The methodology consisted of an evaluation of the sensory and chemical parameters. Chemical analysis of PHE indicated high concentrations of phenolic and triterpens substances, and the absence of steroids. Additionally, we evaluated the acute toxicity of propolis using 48 Swiss male and female mice. The animals received single doses of PHE (1000, 2000 or 4000 mg kg⁻¹) orally and were observed for 14 days. After this period, the mice were sacrificed and the blood was used for biochemical and haematological evaluation. PHE did not induce any death, and the acute treatment significantly reduced serum concentrations of alanine aminotransferase and alkaline phosphatase. The resultant data indicate that PHE from S. aff. postica has low toxicity when used orally, even in high doses.