Biochar amendment for reducing the environmental impacts of reclaimed polluted sediments.

Dredging activities produce large amounts of polluted sediments that require adequate management strategies. Sediment reuse and relocation can involve several environmental issues, such as the release of CO2 and nitrogen compounds in the environment, the transfer of metals to plant tissues and the persistence of phytotoxic compounds. In this framework, the aim of the present work is to evaluate the use of biochar at different doses, in combination with plant growth, to reduce the environmental impacts polluted dredged sediments. Irrespective to the plant treatment, the amendment of the sediment with the lowest dose of biochar (3%) reduced by 25% the CO2 emissions of the substrate, by 89% the substrate carbon loss and by 35% the amount of nitrogen released into the environment (average values of the three plant treatments). The negative priming effect of biochar on organic matter mineralization can be responsible for the beneficial reduction of carbon and nitrogen release in the environment. The lack of similar effects observed at the higher biochar doses can depend on the low albedo of the biochar particles, causing the substrate warming (+1 °C for highest biochar dose) and accelerating the organic matter mineralization. Finally, shrub growth in combination with 3% biochar was able to offset the CO2 emission of the sediment and to reduce the amount of nitrogen lost. This work provides new insight on the potential benefit related to the biochar amendment of organic matter-rich dredged sediments, suggesting that the use of moderate dose of wood biochar in combination with shrub plantation can reduce the release of CO2 and nitrogen compounds in the environment.

Enhancement of ecosystem carbon uptake in a dry shrubland under moderate warming: The role of nitrogen-driven changes in plant morphology.

Net ecosystem CO2 exchange is the result of net carbon uptake by plant photosynthesis and carbon loss by soil and plant respiration. Temperature increases due to climate change can modify the equilibrium between these fluxes and trigger ecosystem-climate feedbacks that can accelerate climate warming. As these dynamics have not been well studied in dry shrublands, we subjected a Mediterranean shrubland to a 10-year night-time temperature manipulation experiment that analyzed ecosystem carbon fluxes associated with dominant shrub species, together with several plant parameters related to leaf photosynthesis, leaf morphology, and canopy structure. Under moderate night-time warming (+0.9°C minimum daily temperature, no significant reduction in soil moisture), Cistus monspeliensis formed shoots with more leaves that were relatively larger and denser canopies that supported higher plant-level photosynthesis rates. Given that ecosystem respiration was not affected, this change in canopy morphology led to a significant enhancement in net ecosystem exchange (+47% at midday). The observed changes in shoot and canopy morphology were attributed to the improved nutritional state of the warmed plants, primarily due to changes in nitrogen cycling and higher nitrogen resorption efficiency in senescent leaves. Our results show that modifications in plant morphology triggered by moderate warming affected ecosystem CO2  fluxes, providing the first evidence for enhanced daytime carbon uptake in a dry shrubland ecosystem under experimental warming.

Lead transfer into the vegetation layer growing naturally in a Pb-contaminated site.

The lead was one of the main elements in the glazes used to colour ceramic tiles. Due to its presence, ceramic sludge has been a source of environmental pollution since this dangerous waste has been often spread into the soil without any measures of pollution control. These contaminated sites are often located close to industrial sites in the peri-urban areas, thus representing a considerable hazard to the human and ecosystem health. In this study, we investigated the lead transfer into the vegetation layer (Phragmites australis, Salix alba and Sambucus nigra) growing naturally along a Pb-contaminated ditch bank. The analysis showed a different lead accumulation among the species and their plant tissues. Salix trees were not affected by the Pb contamination, possibly because their roots mainly develop below the contaminated deposit. Differently, Sambucus accumulated high concentrations of lead in all plant tissues and fruits, representing a potential source of biomagnification. Phragmites accumulated large amounts of lead in the rhizomes and, considering its homogeneous distribution on the site, was used to map the contamination. Analysing the Pb concentration within plant tissues, we got at the same time information about the spread, the history of the contamination and the relative risks. Finally, we discussed the role of natural recolonizing plants for the soil pollution mitigation and their capacity on decreasing soil erosion and water run-off.

Effects of rain shortage on carbon allocation, pools and fluxes in a Mediterranean shrub ecosystem - a 13C labelling field study.

Hydrological cycle is expected to become the primary cause of ecosystem's degradation in near future under changing climate. Rain manipulation experiments under field conditions provide accurate picture on the responses of biotic processes to changed water availability for plants. A field experiment, mimicking expected changes in rain patterns, was established in a Mediterranean shrub community at Porto Conte, Italy, in 2001. In November 2011 Cistus monspeliensis, one of the dominating shrub species in the Mediterranean basin, was 13C labelled on plots subjected to extended rain shortage period and on control non manipulated plots. Carbon (C) allocation was traced by 13C dynamics in shoots, shoot-respired CO2, roots, microbial biomass, K2SO4-extractable C and CO2 respired from soil. Most of the recovered 13C (60%) was respired by shoots within 2weeks in control plots. In rain shortage treatment, 13C remained incorporated in aboveground plant parts. Residence time of 13C in leaves was longer under the rain shortage because less 13C was lost by shoot respiration and because 13C was re-allocated to leaves from woody tissues. The belowground C sink was weak (3-4% of recovered 13C) and independent on rain manipulation. Extended rain shortage promoted C exudation into rhizosphere soil in expense of roots. Together with lowered photosynthesis, this "save" economy of new C metabolites reduces the growing season under rain shortage resulting in decrease of shrub cover and C losses from the system on the long-term.

Shrubland primary production and soil respiration diverge along European climate gradient.

Above- and belowground carbon (C) stores of terrestrial ecosystems are vulnerable to environmental change. Ecosystem C balances in response to environmental changes have been quantified at individual sites, but the magnitudes and directions of these responses along environmental gradients remain uncertain. Here we show the responses of ecosystem C to 8-12 years of experimental drought and night-time warming across an aridity gradient spanning seven European shrublands using indices of C assimilation (aboveground net primary production: aNPP) and soil C efflux (soil respiration: Rs). The changes of aNPP and Rs in response to drought indicated that wet systems had an overall risk of increased loss of C but drier systems did not. Warming had no consistent effect on aNPP across the climate gradient, but suppressed Rs more at the drier sites. Our findings suggest that above- and belowground C fluxes can decouple, and provide no evidence of acclimation to environmental change at a decadal timescale. aNPP and Rs especially differed in their sensitivity to drought and warming, with belowground processes being more sensitive to environmental change.

Increased sensitivity to climate change in disturbed ecosystems.

Human domination of the biosphere includes changes to disturbance regimes, which push many ecosystems towards early-successional states. Ecological theory predicts that early-successional ecosystems are more sensitive to perturbations than mature systems, but little evidence supports this relationship for the perturbation of climate change. Here we show that vegetation (abundance, species richness and species composition) across seven European shrublands is quite resistant to moderate experimental warming and drought, and responsiveness is associated with the dynamic state of the ecosystem, with recently disturbed sites responding to treatments. Furthermore, most of these responses are not rapid (2-5 years) but emerge over a longer term (7-14 years). These results suggest that successional state influences the sensitivity of ecosystems to climate change, and that ecosystems recovering from disturbances may be sensitive to even modest climatic changes. A research bias towards undisturbed ecosystems might thus lead to an underestimation of the impacts of climate change.