Eutrophication driven macrophyte-derived organic matter decomposition to methane emission relates to co-metabolism effect in freshwater sediments.

Lakes and wetlands play pivotal roles in global organic matter storage, receiving significant inputs of organic material. However, the co-metabolic processes governing the decomposition of these organic materials and their impact on greenhouse gas emissions remain inadequately understood. This study aims to assess the effects of mixed decomposition involving macrophytes and cyanobacteria on carbon emissions. A series of microcosms was established to investigate the decomposition of macrophyte residues and algae over a period of 216 days. A two-component kinetic model was utilized to estimate methane (CH4) production rates. Gas isotope technology was employed to discern the contributions of CH4 produced by macrophyte residues or algae. Quantitative PCR and analysis of 16S rRNA gene amplicons were employed to assess changes in functional genes and microbial communities. There were significant differences in the cumulative carbon release from the decomposition of different plant types due to the addition of carbon sources. After adding algae, the cumulative emission of CH4 increased significantly. The δ13C-CH4 partitioning indicated that CH4 originated exclusively from the fresh organic carbon of macrophyte residues, while it shifted to algae source after adding algae. The synergistic effect of the mixed decomposition on the CH4 emissions was greater than the sum of the individual decompositions. The microbial community richness was higher in the single plant residue treatment compared to the mixed treatment with algae addition, while microbial evenness in the sediment increased steadily in each treatment. Our findings emphasize the pronounced co-metabolic effect observed during the mixed decomposition of macrophytes and cyanobacteria.

Emission dynamics of greenhouse gases regulated by fluctuation of water level in river-connected wetland.

The application of reservoirs in the upper reaches of rivers will change the hydrological rhythm of river-connected wetlands in the lower reaches, causing changes in the distribution of wetland vegetation. The differences of carbon and nitrogen sequestration and emission potential in different vegetations may lead to the dynamics of greenhouse gas emissions from wetlands during hydrological periods. For a wetland connected to the Yangzi River, China, the dynamic changes of vegetation and water areas were identified by remote sensing, and the water level, the emission fluxes of greenhouse gases and the functional bacteria of carbon and nitrogen in soil were measured in-situ. Compared with drought period, the area of phragmites zone in flooding period increased by 28.2%, while the areas of carex and phalaris zones decreased by 42.9%. The carbon and nitrogen accumulation in the soil of phragmites zone is the highest, while the cumulative amount of phalaris is the lowest. The emission fluxes of CH4 and N2O in mud/water and various vegetations were positively correlated with water level and reached the maximum during flooding period. Although the global warming potential of mud/water was highest than that of vegetations, carex zone had the highest warming potential among vegetation zones. CH4 contributes 8-37 times as much as N2O to global warming potential in the wetland. The increase of flooding time promoted the emissions of CH4 and N2O in the wetland. The anaerobic condition caused by flooding stimulated the activities of denitrifying and methanogenic bacteria, thus increasing the emission of greenhouse gases. The sequestrations and emissions of carbon and nitrogen regulated by a reservoir in the upstream suggest that the operation of water conservancies should be considered to alleviate the greenhouse gas emission from river-connected wetland.

Biofilter-constructed wetland-trophic pond system: A new strategy for effective sewage treatment and agricultural irrigation in rural area.

Artificial ecosystems with high biological complexity are generally considered to be efficient in metabolizing substances and resistant to temperature shock. In this study, a novel near-natural system (BCT system), which consisted of simple biofilter, constructed wetland and trophic biology pond, was conducted to treat rural sewage in situ for irrigation into farmland. Water quality related to carbon and nutrients and microbial community were analyzed along the system to reveal the effect of each unit. The annual average removals of BCT system for TN, NH4+-N, TP and COD could reach 46.53%, 52.18%, 41.48%, and 53.21%, respectively. There was no significant decrease for removal efficiencies from high temperature period (HTP, ≥15 °C) to low temperature period (LTP, <15 °C). In LTP, the trophic pond (TRP) removed 34.85% of TN, 33.93% of NH4+-N, 13.71% of TP and 18.77% of COD, while the removal efficiencies of constructed wetland fluctuated greatly. The TRP facilitated the BCT system to maintain the removal capability during low temperature period. The relative abundance of denitrification functional genes in TRP increased nearly tenfold from HTP to LTP. The effluent quality from the system can meet the agricultural irrigation standards, demonstrating the effect of BCT system on sewage treatment and agricultural irrigation in rural area.

Synergistic Effects of Aquatic Plants and Cyanobacterial Blooms on the Nitrous Oxide Emission from Wetlands.

Wetlands provide a habitat for the symbiosis of multiple plants and play a significant role in global N2O emissions. The metabolic traits and effects on microorganisms, which regulate the conversion of nitrogen to N2O, varies with plant species. The frequent occurrences of cyanobacterial blooms in wetlands can also have a positive or negative effect on denitrification, entangling N2O emissions. In situ observations of the Dongting Lake reveal that the fluxes in N2O emissions vary with the vegetation. Maximum emissions occurred in the mud flat, while the zone with the minimum emissions was populated with carex. In 210-day batch cultures, the addition of cyanobacteria synergistically enhanced N2O production during the degredation of phalaris and reed. The abundance of the nirS and nirK genes decreased over time except in the phalaris-algae group. To mitigate the N2O emissions from wetlands, the macrophyte communities need to be protected, and the cyanobacterial blooms need to be avoided by reducing the nitrogen pollution.

Nitrogen removal enhanced by benthic bioturbation coupled with biofilm formation: A new strategy to alleviate freshwater eutrophication.

Excessive nitrogen input into the water caused eutrophication thereby reducing biodiversity and degrades freshwater function. Nitrogen pollution in sediments is one key reason that makes eutrophication difficult to control. The physicochemical technologies such as dredging and coverage for sediment pollution easily destroyed and homogenized aquatic habitats. To alleviate freshwater eutrophication in ecological way, this work combined the functions of bioturbation and biofilm to test their effect on the removal of nitrogen from sediment and water. The total nitrogen removal by employing the coupled function (bioturbation + biofilm, SCB) was greater than that of the single function (bioturbation or biofilm). The mean efficiency of total nitrogen removal in SCB treatment was 3.19 times that of the control without chironomids nor biofilm medium. Chironomid bioturbation promoted nitrogen release from sediments to the overlying water. Biofilm enhanced the conversion and removal of nitrogen stirred up by chironomids, resulting the lowest concentration of total nitrogen in overlying water of SCB treatment. The enhancement of nitrogen removal may be due to the coupled function increased the abundance of denitrifying and anammox functional bacteria in sediment and biofilm. Therefore, the method of combining benthic animals with biofilm medium is not only a viable solution for reducing sedimentary nitrogen loading in freshwater ecosystems, but also a solution to mitigate eutrophication in the overlying water. The restoration and management for aquatic ecosystems should consider protecting habitat for benthic organisms while maintaining heterogeneity for biofilm.

Dominant Contribution of a Lake's Internal Pollution to Eutrophication During Rapid Urbanization.

Artificial lakes that form during rapid urbanization often fail to achieve their desired functions, and gradually become eutrophic. Whether the external discharge or internal release of nutrients dominates the eutrophication of urban lakes has rarely been reported. In this study, a lake that had been formed during ten years of urbanization had become hyper-eutrophic. TP mainly contributed to the eutrophication and algal bloom in the lake. While the release potential of TP fluctuated, TN, particularly NH3-N, was constantly released from the sediment. Concentrations of anthropogenic metals (Pb, Cu and Cr) increased with the increasing depth of the sediment. Even for a lake that had formed rapidly in a short period, the internal phosphorus released from sediment was 1.9-times higher than that of the external discharge. The dominating contribution of internal pollution from sediment requires more attention to restore and manage these urban waters.

Cyanobacteria decay alters CH4 and CO2 produced hotspots along vertical sediment profiles in eutrophic lakes.

Cyanobacteria-derived organic carbon has been reported to intensify greenhouse gas emissions from lacustrine sediments. However, the specific processes of CH4 and CO2 production and release from sediments into the atmosphere remain unclear, especially in eutrophic lakes. To investigate the influence of severe cyanobacteria accumulation on the production and migration of sedimentary CH4 and CO2, this study examined the different trophic level lakes along the middle and lower reaches of the Yangtze River. The results demonstrated that eutrophication amplified CH4 and CO2 emissions, notably in Lake Taihu, where fluxes peaked at 929.9 and 7222.5 µmol/m2·h, mirroring dissolved gas levels in overlying waters. Increased sedimentary organic carbon raised dissolved CH4 and CO2 concentrations in pore-water, with isotopic tracking showing cyanobacteria-derived carbon specifically elevated CH4 and CO2 in surface sediment pore-water more than in deeper layers. Cyanobacteria-derived carbon deposition on surface sediment boosted organic carbon and moisture levels, fostering an anaerobic microenvironment conducive to enhanced biogenic CH4 and CO2 production in surface sediments. In the microcosm systems with the most severe cyanobacteria accumulation, average CH4 and CO2 concentrations in surface sediments reached 6.9 and 2.3 mol/L, respectively, surpassing the 4.7 and 1.4 mol/L observed in bottom sediments, indicating upward migration of CH4 and CO2 hotspots from deeper to surface layers. These findings enhance our understanding of the mechanisms underlying lake sediment carbon emissions induced by eutrophication and provide a more accurate assessment of lake carbon emissions.

Occurrence of per- and polyfluoroalkyl substances (PFAS) in municipal solid waste landfill leachates from western China.

Landfill leachate has been documented as a significant source of trace organic pollutants, comprising an expansive family of per- and polyfluoroalkyl substances (PFAS). This study presents the findings on the distribution of 13 perfluoroalkyl carboxylates (PFCAs) and 4 perfluoroalkyl sulfonates (PFSAs) in leachates from 6 municipal solid waste (MSW) landfills in western China. The total concentrations of 17 PFAS in sampled leachates ranged from 1805 to 43,310 ng/L, and 15 compounds were detected in all samples. The short-chain compounds perfluorobutane sulfonate (PFBS, mean mass fraction 23.1%) and perfluorobutyric acid (PFBA, mean mass fraction 20.6%) were dominant. There were higher PFAS concentrations in leachates from operating landfills (mean: 12,194 ng/L) compared to closed landfills (mean: 2747 ng/L), but there was no significant difference between young (< 10 years) and old landfills (> 10 years). Moderate to weak correlations were observed between PFAS concentrations and leachate properties, e.g., TN, NH4+-N, TOC, and pH. This is the first report on the distribution of PFAS in landfill leachates from western China. The results have identified landfill leachate as an underestimated source of PFAS in the environment and have contributed to a more comprehensive evaluation on PFAS presence across China.

Dam operation altered profiles of per- and polyfluoroalkyl substances in reservoir.

Information on the impact of dam operation on per- and polyfluoroalkyl substances (PFASs) distribution in reservoirs is very limited. In the present study, water, riparian soils and floating wastes samples were collected from the Three Gorges Reservoir, China during the storage and the discharge periods to characterize the PFASs distribution. The total PFASs concentrations of water samples in the storage period (50.4-146 ng/L) were 4.7 times higher than those in the discharge period (1.40-38.6 ng/L). The main types of PFASs in water samples changed from PFOA in the discharge period to short-chain species in the storage period. The main analogues in riparian soils and floating wastes were PFOA and PFOS. Wastes contributed little to PFASs mass in the reservoir, while PFASs accumulated in soils accounted for 49.7 % of the total mass when the riparian zone was submerged during the storage period. Changes in profiles of PFASs caused by dam operation suggested that the potential water safety and the shift of riparian soils between source and sink of PFASs may vary with the annual operation cycle of dam. The water resources protection in reservoirs needs strategies that consider the variation of dam operation cycle.