Nickel Oxide Nanoparticles Improve Soybean Yield and Enhance Nitrogen Assimilation.

Nickel (Ni) is a trace element beneficial for plant growth and development and could improve crop yield by stimulating urea decomposition and nitrogen-fixing enzyme activity. A full life cycle study was conducted to compare the long-term effects of soil-applied NiO nanoparticles (n-NiO), NiO bulk (b-NiO), and NiSO4 at 10-200 mg kg-1 on plant growth and nutritional content of soybean. n-NiO at 50 mg kg-1 significantly promoted the seed yield by 39%. Only 50 mg kg-1 n-NiO promoted total fatty acid content and starch content by 28 and 19%, respectively. The increased yield and nutrition could be attributed to the regulatory effects of n-NiO, including photosynthesis, mineral homeostasis, phytohormone, and nitrogen metabolism. Furthermore, n-NiO maintained a Ni2+ supply for more extended periods than NiSO4, reducing potential phytotoxicity concerns. Single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS) for the first time confirmed that the majority of the Ni in seeds is in ionic form, with only 28-34% as n-NiO. These findings deepen our understanding of the potential of nanoscale and non-nanoscale Ni to accumulate and translocate in soybean, as well as the long-term fate of these materials in agricultural soils as a strategy for nanoenabled agriculture.

Insight into the formation and biological effects of natural organic matter corona on silver nanoparticles in water environment using biased cyclical electrical field-flow fractionation.

Natural organic matter (NOM) readily interacts with nanoparticles, leading to the formation of NOM corona structures on their surface. NOM corona formation is closely related to the surface coatings and bioavailability of nanoparticles. However, the mechanism underlying NOM corona formation on silver nanoparticles (AgNPs) remains largely unknown due to the lack of effective analytical methods for identifying the changes in the AgNP surface. Herein, the separation ability of biased cyclical electrical field-flow fractionation (BCyElFFF) for same-sized polyvinyl pyrrolidone-coated and poly(ethylene glycol)-coated silver nanoparticles (AgNPs) with different electrophoretic mobilities was evaluated under various electrical conditions. Then, the mechanism behind the NOM corona formation on these AgNP surfaces was elucidated based on the changes in the elution time and off-line characterization of the collected fractions during their elution time in a BCyElFFF run. Finally, the survival rates of E. coli exposed to polyvinyl pyrrolidone-coated and poly(ethylene glycol)-coated AgNPs with or without NOM collected during repeated BCyElFFF runs were observed to increase with increasing NOM concentration, clearly demonstrating the negative effect of NOM corona structures on the bioavailability of AgNPs. These findings highlight the powerful separation and isolation ability of BCyElFFF in studying the transformation and fate of nanoparticles in aqueous environments.

Engineered Zn-based nano-pesticides as an opportunity for treatment of phytopathogens in agriculture.

People's desire for food has never slowed, despite the deterioration of the global agricultural environment and the threat to food security. People rely on agrochemicals to ensure normal crop growth and to relieve the existing demand pressure. Phytopathogens have acquired resistance to traditional pesticides as a result of pesticdes' abuse. Compared with traditional formulations, nano-pesticides have superior antimicrobial performance and are environmentally friendly. Zn-based nanoparticles (NPs) have shown their potential as strong antipathogen activity. However, their full potential has not been demonstrated yet. Here, we analyzed the prerequisites for the use of Zn-based NPs as nano-pesticides in agriculture including both intrinsic properties of the materials and environmental conditions. We also summarized the mechanisms of Zn-based NPs against phytopathogens including direct and indirect strategies to alleviate plant disease stress. Finally, the current challenges and future directions are highlighted to advance our understanding of this field and guide future studies.

Tracking the dissolution behavior of zinc oxide nanoparticles in skimmed milk powder solutions.

Zinc oxide nanoparticles (ZnO NPs) are recently recommended as food additives owing to their outstanding nutritive function. Therefore, understanding their comprehensive information and stability in food samples is highly necessitated. However, the characterization of ZnO NPs in the complex food matrices remains a great challenge, limiting an in-depth understanding of their transformation during food storage. In this study, the hollow fiber flow field-flow fractionation was combined with UV-Vis absorption spectroscopy and inductively coupled plasma optical emission spectroscopy to assess the dissolution behaviors of ZnO NPs in skimmed milk powder solutions by monitoring the changes in the residual ZnO NPs and the amount of dissolved Zn(II) ions. The simultaneous characterization of these two Zn species in skimmed milk powder solutions was achieved without the need for tedious sample pretreatments, and the dissolution of ZnO NPs in skimmed milk powder solutions had time- and temperature-dependent behaviors.

Microbial electro-Fenton: A promising system for antibiotics resistance genes degradation and energy generation.

The widespread use of antibiotics has accelerated the development of antibiotic resistance genes (ARGs), which are now recognized as emerging environmental contaminants that pose a high risk to public health. In this study, simultaneous antibiotic and ARGs removal and bioelectricity generation was explored in a microbial electro-Fenton system using erythromycin (ERY) as a model antibiotic compound. The results showed that ERY could be degraded, with an average removal efficiency of 88.73% in 48 h, and the average removal efficiency of chemical oxygen demand in the microbial electro-Fenton with 50 µg L-1 ERY reached 86.84% in 48 h, which was lower than that in the control group (89.11%). The produced ARGs were analyzed and degraded in a cathode chamber. The quantity of ermB was significantly reduced, with log removal reaching a value of 1.96. More importantly, all erm genes (ermB, ermC, ermG) showed a tendency to be degraded. Furthermore, the maximum power density obtained with respect to the electrode area was 0.193 W m-2 when ERY was added, corresponding to a current density of 0.583 A m-2 (external resistor = 1000 Ω), which was 14% larger than that of the control group (0.169 W m-2). The results of this study demonstrate the potential of microbial electro-Fenton for ERY and ARGs removal.