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1.
Molecules ; 29(12)2024 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-38930789

RESUMEN

The aerobic oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) plays a pivotal role in the synthesis of renewable, biodegradable plastics and sustainable chemicals. Although supported gold nanoclusters (NCs) exhibit significant potential in this process, they often suffer from low selectivity. To address this challenge, a series of gold-M (M means Ni, Fe, Cu, and Pd) bimetallic NCs catalysts were designed and synthesized to facilitate the selective oxidation of HMF to FDCA. Our findings indicate that the introduction of doped metals, particularly Ni and Pd, not only improves the reaction rates for HMF tandem oxidation but also promotes high yields of FDCA. Various characterizations techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), in situ diffuse reflectance infrared Fourier transform spectroscopy of CO adsorption (CO-DRIFTS), and temperature-programmed desorption of oxygen (O2-TPD), were employed to scrutinize the structural and electronic properties of the prepared catalysts. Notably, an electronic effect was observed across the Au-based bimetallic catalysts, facilitating the activation of reactant molecules and enhancing the catalytic performance. This study provides valuable insights into the alloy effects, aiding in the development of highly efficient Au-based bimetallic catalysts for biomass conversions.

2.
Angew Chem Int Ed Engl ; 63(27): e202402693, 2024 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-38586976

RESUMEN

As an effective method to modulate the physicochemical properties of materials, crystal phase engineering, especially hetero-phase, plays an important role in developing high-performance photocatalysts. However, it is still a huge challenge but significant to construct porous hetero-phase nanostructures with adjustable band structures. As a kind of unique porous crystalline materials, metal-organic frameworks (MOFs) might be the appropriate candidate, but the MOF-based hetero-phase is rarely reported. Herein, we developed a secondary building unit (SBU) regulating strategy to prepare two crystal phases of Ti-MOFs constructed by titanium and 1,4-dicarboxybenzene, i.e., COK and MIL-125. Besides, COK/MIL-125 hetero-phase was further constructed. In the photocatalytic hydrogen evolution reaction, COK/MIL-125 possessed the highest H2 yield compared to COK and MIL-125, ascribing to the Z-Scheme homojunction at hetero-phase interface. Furthermore, by decorating with amino groups (i.e., NH2-COK/NH2-MIL-125), the light absorbing capacity was broadened to visible-light region, and the visible-light-driven H2 yield was greatly improved. Briefly, the MOF-based hetero-phase possesses periodic channel structures and molecularly adjustable band structures, which is scarce in traditional organic or inorganic materials. As a proof of concept, our work not only highlights the development of MOF-based hetero-phase nanostructures, but also paves a novel avenue for designing high-performance photocatalysts.

3.
J Opt Soc Am A Opt Image Sci Vis ; 40(1): 10-20, 2023 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-36607070

RESUMEN

Diffuse optical tomography (DOT) is a non-invasive imaging modality that uses near-infrared light to probe the optical properties of tissue. In conventionally used deterministic methods for DOT inversion, the measurement errors were not taken into account, resulting in unsatisfactory noise robustness and, consequently, affecting the DOT image reconstruction quality. In order to overcome this defect, an extended Kalman filter (EKF)-based DOT reconstruction algorithm was introduced first, which improved the reconstruction results by incorporating a priori information and measurement errors to the model. Further, to mitigate the instability caused by the ill-condition of the observation matrix in the tomographic imaging problem, a new, to the best of our knowledge, estimation algorithm was derived by incorporating Tikhonov regularization to the EKF method. To verify the effectiveness of the EKF algorithm and Tikhonov regularization-based EKF algorithm for DOT imaging, a series of numerical simulations and phantom experiments were conducted, and the experimental results were quantitatively evaluated and compared with two conventionally used deterministic methods involving the algebraic reconstruction technique and Levenberg-Marquardt algorithm. The results show that the two EKF-based algorithms can accurately estimate the location and size of the target, and the imaging accuracy and noise robustness are obviously improved. Furthermore, the Tikhonov regularization-based EKF obtained optimal parameter estimations, especially under the circumstance of low absorption contrast (1.2) and high noise level (10%).


Asunto(s)
Procesamiento de Imagen Asistido por Computador , Tomografía Óptica , Procesamiento de Imagen Asistido por Computador/métodos , Tomografía Óptica/métodos , Algoritmos
4.
J Environ Manage ; 331: 117299, 2023 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-36642053

RESUMEN

Advanced nitrogen removal faces the challenges of high operational cost resulted from the additional carbon source and secondary pollution caused by inaccurate carbon source dosage in municipal wastewater. To address these problems, a novel carbon source was developed, which was the oxidation products of refractory organic matters in the secondary effluent of municipal wastewater treatment plant (MWWTP) by in-situ generated biogenic manganese oxides (BMOs) in the denitrifying filter. In the steady phase, the effluent chemical oxygen demand (CODcr), NO3--N and total nitrogen (TN) in the denitrifying filter 2# with BMOs was 11.27, 9.03 and 10.36 mg/L, and the corresponding removal efficiency was 54.79%, 51.85% and 48.03%, respectively, which was significantly higher than those in the control denitrifying filter 1# that the removal efficiency of CODcr, NO3--N and TN was only 32.30%, 28.58% and 29.36%, respectively. Kinds of denitrifying bacteria (Candidatus Competibacter, Defluviicoccus, Dechloromonas, Candidatus Competibacter, Dechloromonas, Pseudomonas, Thauera, Acinetobacter, Denitratisoma, Anaerolineae and Denitratisoma) and anammox bacteria (Pirellula, Gemmata, Anammoximicrobium and Brocadia) were identified in the denitrifying filters 1# and 2#, which explained why the actual CODcr consumption (1.55 and 1.44 mg) of reducing 1 mg NO3--N was much lower than the theoretical CODcr consumption. While manganese oxidizing bacteria (MnOB, Bacillus, Crenothrix and Pedomicrobium) was only identified in the denitrifying filter 2#. This novel technology presented the advantages of no additional carbon source, low operational cost and no secondary pollution. Therefore, the novel technology has superlative application value and broad application prospect.


Asunto(s)
Manganeso , Microbiota , Desnitrificación , Nitrógeno , Óxidos , Oxidación-Reducción , Bacterias , Carbono , Reactores Biológicos/microbiología , Aguas del Alcantarillado
5.
Chem Biodivers ; 19(7): e202200218, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35689671

RESUMEN

Three new alkamides, achilleamide B-D (1-3) along with five known alkamides (4-8) were isolated from the aerial parts of Achillea alpina L. Structures were elucidated by spectroscopic analysis. Modified Mosher's method and electronic circular dichroism (ECD) calculations were introduced for the absolute configuration of 3. The neuroprotective effects of all the compounds were evaluated by 6-hydroxydopamine (6-OHDA)-induced cell death in human neuroblastoma SH-SY5Y cells, with concentration for 50 % of maximal effect (EC50 ) values of 3.16-24.75 µM, and the structure-activity relationship was conducted.


Asunto(s)
Achillea , Neuroblastoma , Fármacos Neuroprotectores , Achillea/química , Humanos , Estructura Molecular , Fármacos Neuroprotectores/química , Componentes Aéreos de las Plantas/química
6.
Zhongguo Zhong Yao Za Zhi ; 47(18): 4919-4926, 2022 Sep.
Artículo en Zh | MEDLINE | ID: mdl-36164901

RESUMEN

The present study designed and prepared near-infrared responsive sinomenine hydrochloride(SIN) reservoir microneedles and evaluated the feasibility of this type of microneedles in increasing the drug loading and transdermal absorption by characterizing their mechanical properties and in vitro release characteristics.SIN was selected as the model drug, and methoxy poly(ethylene glycol) poly(caprolactone)(mPEG-PCL) copolymers and indocyanine green(ICG) were employed as amphiphilic block copolymers and light inductor to prepare near-infrared responsive nanoparticles.Based on the preparation principle of bubble microneedles, near-infrared responsive SIN reservoir microneedles were designed and prepared.The features of the near-infrared responsive SIN reservoir microneedles were characterized by measuring the morphology, length, mechanical properties, and skin penetration of microneedles.Meanwhile, the drug release performance of reservoir microneedles was evaluated by in vitro release assay.The results showed that the prepared SIN microneedles were conical, with an exposed tip height of about 650 µm.Each needle could load about 0.5 mg of drugs per square centi-meter, and this type of microneedle showed good mechanical properties and performance in skin penetration.The results of the in vitro release assay showed that the 24 h cumulative release per unit area and release rate of the microneedle were 825.61 µg·cm~(-2) and 74.3%, respectively, which indicated that its release kinetics was in line with the first-order kinetic model.This study preliminarily proved that the reservoir microneedle could effectively increase the drug loading with good mechanical properties and release perfor-mance.


Asunto(s)
Verde de Indocianina , Morfinanos , Sistemas de Liberación de Medicamentos/métodos , Liberación de Fármacos , Agujas , Polietilenglicoles
7.
Zhongguo Zhong Yao Za Zhi ; 45(9): 2138-2143, 2020 May.
Artículo en Zh | MEDLINE | ID: mdl-32495563

RESUMEN

This study aims to establish an HPLC method for the simultaneous determination of 6 main components, including chlorogenic acid, 3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid, pellitorine and neopellitorine B in Achil-leae Herba. HPLC analysis was performed on a Merck Purospher STAR RP-18 endcapped(4.6 mm×250 mm, 5 µm), with a mobile phase consisting of 0.05% phosphoric acid solution(A)-acetonitrile(B) at a flow rate of 1 mL·min~(-1)(0-7 min,12%-14% B;7-10 min,14%-17% B;10-25 min,17%-22% B;25-35 min,22%-35% B;35-51 min,35%-80% B;51-60 min,80%-90% B). The detection wavelength was 254 nm and the column temperature maintained at 30 ℃, and the injection volume was 5 µL. The standard curves revealed a good linear relationship. The contents of 6 components were 0.404%-2.116% for chlorogenic acid, 0.160%-0.892% for 3,4-dicaffeoylquinic acid, 0.608%-1.464% for 3,5-dicaffeoylquinic acid, 0.168%-0.868% for 4,5-dicaffeoylquinic acid, 0.122%-1.234% for pellitorine, 0.065%-0.312% for neopellitorine B, respectively. Both cluster and principal component analysis can distinguish the research data in anthesis and pre-anthesis by software Chempattern. There were obviously differences in the different harvest time. Therefore, attention should be paid to the harvesting time of the herb. The method can be used to determine the contents of six main components, and can provide reference for the improvement of quality standard of Achilleae Herba.


Asunto(s)
Medicamentos Herbarios Chinos , Ácido Clorogénico , Cromatografía Líquida de Alta Presión , Análisis de Componente Principal
8.
Zhongguo Zhong Yao Za Zhi ; 45(4): 910-915, 2020 Feb.
Artículo en Zh | MEDLINE | ID: mdl-32237493

RESUMEN

This work aims to establish an HPLC specific chromatogram and determine six components of Vernonia anthelmintica with chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, scutellarein and luteolin as index components. HPLC analysis was performed on a Waters Xbridge C_(18) column(4.6 mm×250 mm, 5 µm) with gradient elution of acetonitrile-0.05% trifluoroacetic acid solution at a flow rate of 1.0 mL·min~(-1). The detection wave length was 360 nm and the column temperature was 30 ℃. Chemometrics software Chempattern was employed to analyze the data. HPLC specific chromatogram of V. anthelmintica was established and six characteristic peaks were marked. Six characteristic peaks were simultaneously determined by HPLC within 50 min. The contents of the six components in 13 batch samples of V. anthelmintica were 0.14%-0.68%, 0.44%-0.74%, 0.63%-1.01%, 0.14%-0.71%, 0.15%-0.26% and 0.010%-0.030%, respectively. The HPLC specific chromatogram of V. anthelmintica, together with determination of six components showed strong specificity, and it can be used for the quality control of the crude drug.


Asunto(s)
Medicamentos Herbarios Chinos/química , Fitoquímicos/análisis , Vernonia/química , Cromatografía Líquida de Alta Presión , Control de Calidad
9.
Zhongguo Zhong Yao Za Zhi ; 43(8): 1662-1666, 2018 Apr.
Artículo en Zh | MEDLINE | ID: mdl-29751714

RESUMEN

The present study is to develop an HPLC-ELSD method for simultaneous determination of three pairs of triterpenoid isomers, Ilexsaponin A1, Ilexhainanoside D, Ilexgenin A, 3ß, 19α-dihydroxyolean-12-ene-24, 28-dioic acid (ilexhainanin E) ursolic acid and oleanic acid in the leaf of Ilex hainanensis, which could provide evidence to the quality control of this herb. The six constituents were measured on a Waters XBridge C18 column (4.6 mm×250 mm, 5 µm), with a mobile phase consisting of methanol (A)- 0.5% formic acid in water (B) at a flow rate of 1.0 mL·min⁻¹ (0-18 min,70%-85% A,18-20 min,85%-95% A;20-35 min,95% A). The carrier gas was N2, and the pressure was 2.8 L·min⁻¹. The drift tube in this experiment were set at 70 °C. The injection volume was 10 µL. The contents of the six triterpenoids in 6 samples were 3.7-8.5, 10.3 -22.1, 2.8-5.9, 7.8-14.1, 2.6-3.8 and 8.8-11.9 mg·g⁻¹, respectively. The established method is proved to be accurate and sensitive for the determination of triterpenoids in Ilicis Hainanensis Folium, and may be used for the quality improvement of this herb.


Asunto(s)
Ilex , Cromatografía Líquida de Alta Presión , Hojas de la Planta
10.
Naunyn Schmiedebergs Arch Pharmacol ; 397(10): 8009-8022, 2024 10.
Artículo en Inglés | MEDLINE | ID: mdl-38775855

RESUMEN

This research aimed to explore the impact of nodosin on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) in rats. The study involved administering nodosin orally at doses of 2 and 4 mg/kg body weight orally to rats for 7 days before induction of AKI. Toward the end of the study, urine, blood, and kidneys were gathered from the rats to undergo biochemical and molecular examination after sacrificing them. Serum Scr, BUN, urine NGAL, and KIM-1 levels were significantly decreased in nodosin-treated AKI rats. Besides, nodosin administration resulted in a significant reduction in kidney MDA and 4-HNE levels. In contrast, antioxidant enzymes such as SOD, CAT, GPx, and GST levels increased, as well as Nrf2, NQO1, and HO-1 levels increased, while Keap-1 mRNA levels decreased in AKI rats. In addition, AKI rats treated with nodosin reversed excessive ferroptosis in the kidneys of LPS-induced AKI rats, as evidenced by increased mRNA and protein levels of GPX4, SLC7A11, and FTH-1. The administration of nodosin significantly reduced levels of inflammatory markers including TLR4, MYD88, NF-κB p65, IkKß, and IL-1ß, while IL-10 levels increased in the AKI-induced rats. Besides, histopathological changes were reduced in AKI-induced rats treated with nodosin. Nodosin proves highly beneficial in safeguarding the kidney from AKI by regulating oxidative stress, inflammation, and ferroptosis. The treatment of AKI could greatly benefit from this option.


Asunto(s)
Lesión Renal Aguda , Ferroptosis , Inflamación , Lipopolisacáridos , Estrés Oxidativo , Animales , Estrés Oxidativo/efectos de los fármacos , Lesión Renal Aguda/inducido químicamente , Lesión Renal Aguda/metabolismo , Lesión Renal Aguda/prevención & control , Lesión Renal Aguda/patología , Lesión Renal Aguda/tratamiento farmacológico , Lipopolisacáridos/toxicidad , Masculino , Ferroptosis/efectos de los fármacos , Ratas , Inflamación/metabolismo , Inflamación/tratamiento farmacológico , Riñón/efectos de los fármacos , Riñón/patología , Riñón/metabolismo , Antioxidantes/farmacología , Ratas Wistar
11.
Immun Inflamm Dis ; 12(3): e1227, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38533915

RESUMEN

BACKGROUND: End-stage renal disease (ESRD) is the final stage of chronic kidney disease (CKD). AIMS: We aimed to analyze the expression differences of serum thrombomodulin (TM), platelet-activating factor (PAF), and P-selectin (CD62P) in patients with autologous arteriovenous fistula (AVF) and the correlation with vascular access function. METHODS: The case data were retrospectively analyzed. Moreover, 160 patients with AVF maintenance hemodialysis were selected as the AVF group, and 150 healthy participants were selected as the healthy control group. According to the function of vascular access, patients in the AVF group were divided into Group A (n = 50, after the first establishment of AVF), Group B (n = 64, normal vascular access function after hemodialysis treatment), and Group C (n = 46, vascular access failure). Pearson analysis was conducted to explore the correlation between serum TM, PAF, CD62P content, and vascular pathological examination indicators, to evaluate the value of TM, PAF, and CD62P levels in predicting vascular access failure in patients with AVF. RESULTS AND DISCUSSION: The serum levels of TM, PAF, and CD62P were positively correlated with the expressions of CD68 and MCP-1, respectively (p < .001). Serum TM was positively correlated with the levels of PAF and CD62P (p < .001), and PAF was positively correlated with the levels of CD62P (p < .001), respectively. Serum levels of TM, PAF and CD62P were risk factors for vascular access failure in AVF patients (p < .05). The area under the curve of serum TM, PAF and CD62P levels in predicting vascular access failure in AVF patients was 0.879. CONCLUSION: The serum levels of TM, PAF, and CD62P in AVF patients were correlated with the vascular access function of AVF patients, which was very important for maintaining the stability of vascular access function, and had certain value in predicting vascular access failure/disorder in AVF patients, and could be popularized and applied.


Asunto(s)
Fístula Arteriovenosa , Derivación Arteriovenosa Quirúrgica , Humanos , Estudios Retrospectivos , Selectina-P , Factor de Activación Plaquetaria , Trombomodulina , Derivación Arteriovenosa Quirúrgica/efectos adversos , Derivación Arteriovenosa Quirúrgica/métodos , Diálisis Renal/efectos adversos , Diálisis Renal/métodos , Fístula Arteriovenosa/etiología
12.
Sci Total Environ ; 898: 165478, 2023 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-37451437

RESUMEN

Mechanical stability of soil aggregates is important for resisting external disturbances in slope soils. Biochar (BC) is widely used in slope remediation. However, biochar application may not be conducive to the formation of mechanical-stable soil aggregates, and the effects of biochar additions on the mechanical stability of soil aggregates in slope restoration remain largely unclear. In this context, an incubation experiment was conducted in this study with four biochar levels added to artificial soil, namely 0 % (BC0), 1.5 % (BC1), 3 % (BC2), and 4.5 % (BC3), corresponding approximately to 0, 0.77, 1.53 and 2.30 M ha-1, respectively. The contributions of different soil aggregate fractions to maintaining the mechanical stability of aggregates, as well as the main influencing factors and pathways of biochar additions on soil aggregate stability in a dynamic renewal process of aggregates, were investigated in this study. The results showed a decreasing trend in the mean weight diameter (MWD) with increasing biochar levels and BC1 has no significant difference with BC0, showing MWD values of 2.74 and 2.75, respectively. In contrast, BC3 is significantly lower MWD value of 2.18. The BC3 exhibited negative impact on the mechanical stability of the aggregates. Redundancy analysis (RDA) showed that large macroaggregates (>5 mm) exhibited a stronger contribution on the aggregate mechanical stability between all soil aggregate fractions. The random forest (RF) algorithm and structural equation modeling (SEM) indicated that microaggregate-associated soil organic carbon (SOC) contents and soil pH values were the main factors driving the changes in the aggregate mechanical stability caused by biochar applications. Indeed, the biochar level of 1.5 % maintained the stability of macroaggregates and increased the microaggregate-associated SOC content by 35.7 %, which was conducive to the formation of microaggregates within macroaggregates. Our study suggests that the application of biochar at a level of 1.5 % is more beneficial for maintaining the mechanical stability of artificial soil aggregates.

13.
Pediatr Investig ; 7(3): 163-167, 2023 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-37736370

RESUMEN

Importance: Nagashima-type palmoplantar keratosis (NPPK) is a hereditary dermatosis mostly caused by a nonsense mutation in SERPINB7. Despite the increasing interest in readthrough gentamicin treatment of NPPK, clinical evidence for this treatment is limited. Objective: This study aimed to provide further evidence for the use of topical gentamicin in the treatment of NPPK in children with nonsense mutations. Methods: We designed a bilaterally controlled study of topical gentamicin ointment. Children diagnosed with NPPK carrying nonsense mutations were enrolled in this study. A 0.1% gentamicin ointment was applied to one hand and an emollient to the other for 3 months. A bilateral comparison of the visual analog scale scores for clinical manifestations and safety was performed. Results: Ten children with NPPK were included in this study. In comparison with the emollient side, the topical gentamicin side showed significant improvements in hyperkeratosis, erythema, maceration, and desquamation after 1 and 3 months of treatment (P < 0.05). However, hyperhidrosis and odor did not improve significantly. No adverse events were observed during the systemic safety monitoring examinations. Interpretation: Topical gentamicin ointment showed good safety in the treatment of NPPK with nonsense mutations, indicating that it is a promising therapeutic choice in children with NPPK.

14.
Environ Sci Pollut Res Int ; 29(34): 51717-51731, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35246795

RESUMEN

The objective of this study was to compare the transformation of by-products between single dielectric barrier discharge (SDBD) and double dielectric barrier discharge (DDBD), to optimize the preparation of graphene-based catalysts and apply them in combination with DBD for volatile organic compound degradation. We compared the degradation performance of SDBD and DDBD, prepared, and characterized graphene-based catalysts. SEM, BET, XRD, and FTIR analyses showed that the morphologies and internal structures of the three catalysts were the best when 0.25 mL of [BMIM]PF6 was added. When MnOx/rGO, FeOx/rGO, and TiOx/rGO were used in combination with DDBD, the degradation rates of benzene were found to be 83.5%, 77.2%, and 63.8%, respectively, whereas the O3 transformation rates were 60%, 79%, and 40%, respectively. Moreover, the NO2 transformation rates were 70%, 55%, and 42.5%, respectively, whereas the NO transformation rates were 69%, 39%, and 33.5%, respectively. The CO2 selectivity was 62%, 51%, and 49%, respectively. MnOx/rGO exhibited superior performance in the degradation of benzene series, NO transformation, NO2 transformation, CO2 selectivity, and energy efficiency. On the other hand, FeOx/rGO exhibited superior performance for O3 transformation. Based upon the XPS analysis, it was found that Mn3O4 and Fe3O4 played a leading role in promoting the degradation of benzene series and the transformation of by-products.


Asunto(s)
Grafito , Compuestos Orgánicos Volátiles , Benceno/química , Dióxido de Carbono , Grafito/química , Dióxido de Nitrógeno
15.
World J Pediatr ; 17(6): 568-575, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34787828

RESUMEN

BACKGROUND: The clinical use of gentamicin always lies in its antimicrobial activity in the past as an aminoglycoside antibiotic. However, in the past decade, there were considerable interests in therapeutic approaches in treating hereditary diseases. Some of the genodermatosis is caused by nonsense mutations that create premature termination codons and lead to the production of truncated or non-functional proteins. Gentamicin could induce readthrough of nonsense mutations and enable the synthesis of full-length proteins. We focus on previous publications on topical application of gentamicin and review its utility in genetic skin diseases. DATA SOURCES: We search the MEDLINE through PubMed, EMBASE databases, and the Clinical Trials Registry Platform from January 1960 to July 2020 using the key search terms "gentamicin, topical gentamicin, genodermatosis, genetic skin diseases". RESULTS: The application of gentamicin in genodermatosis yielded promising results, both in vivo and in vitro, including Nagashima-type palmoplantar keratosis, epidermolysis bullosa, Hailey-Hailey disease, hereditary hypotrichosis simplex of the scalp, etc. CONCLUSIONS: Topical gentamicin is a potential treatment option for genodermatosis caused by nonsense mutation.


Asunto(s)
Gentamicinas , Hipotricosis , Antibacterianos/uso terapéutico , Codón sin Sentido , Gentamicinas/uso terapéutico , Humanos
16.
World J Clin Cases ; 9(16): 3848-3857, 2021 Jun 06.
Artículo en Inglés | MEDLINE | ID: mdl-34141741

RESUMEN

BACKGROUND: Conventional recanalization techniques may fail in patients with completely occluded superior vena cava (SVC). AIM: To analyze the effectiveness and complications of sharp recanalization for completely occluded SVC. METHODS: This was a retrospective study of patients that underwent puncture and recanalization of the SVC between January 2016 and December 2017 at our hospital. Sharp recanalization was performed using the RUPS-100 system. The patients were followed for 12 mo. The main outcomes were the patency rate of SVC and arteriovenous fistula flow during dialysis. RESULTS: The procedure was successful in all 14 patients (100%). Blood pressure in the distal SVC decreased in all 14 cases (100%) from 26.4 ± 2.7 cmH2O to 14.7 ± 1.3 cmH2O (P < 0.05). The first patency rates of the SVC at 24 h and at 3, 6, 9 and 12 mo after sharp recanalization were 100%, 92.9%, 85.7%, 78.6% and 71.4%, respectively. There were two (14.3%) severe, one (7.1%) moderate and one (7.1%) minor complication. The severe complications included one case of pericardial tamponade and one case of hemothorax. CONCLUSION: The results suggest that sharp recanalization can be an additional tool to extend or renew the use of an occluded upper extremity access for hemodialysis. This could be of use in patients with long-term maintenance hemodialysis in whom the maintenance of central venous access is often a challenge.

17.
Bioresour Technol ; 316: 123968, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-32781387

RESUMEN

Test water temperature (TWT) is a significant operational parameter in biofilter. In this study, a pilot-scale biofilter was established to investigate the removal efficiency of CODMn and Mn2+ and the microbial community structure at different TWT. When CODMn and Mn2+ in the influent were 6-8 and 0.9-1.2 mg/L, respectively, the removal rates were 22.61% and 94.28% at the low TWT, while 69.42% and 97.85% at the high TWT, respectively. Biological CODMn and Mn2+ removal followed the first-order reaction, and at the low and high TWT, the k value was 0.00704 and 0.0738 and 0.0313 and 0.113 min-1, respectively. Organic matter oxidizing bacteria (OMOB, Sphingopyxis, Sphingomonas, Amphiplicatus, Novosphingobium, Gemmatimonas, Chryseolinea and Sphingobium) and manganese oxidizing bacteria (MnOB, Hyphomicrobium, Pedomicrobium and Pseudomonas) were coexisted in 0-1.5 m of the biofilter bed at the low and high TWT, and the abundances were not the main factor affecting the removal efficiency, however the activity.


Asunto(s)
Filtración , Microbiota , Hierro , Manganeso , Temperatura
19.
Science ; 384(6699): 967, 2024 May 31.
Artículo en Inglés | MEDLINE | ID: mdl-38815011
20.
Phytomedicine ; 63: 153039, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31387054

RESUMEN

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is becoming a major health concern worldwide. Ilex hainanensis Merr. extract was proved to have anti-inflammation effect on NAFLD, and Ilexhainanoside D (IhD) and ilexsaponin A1 (IsA) were the main triterpenoid saponins extracted from it. PURPOSES: To investigate the hepatoprotective effect of the combination of IhD and IsA (IIC) against NAFLD and discuss the potential mechanisms. METHODS: Male C57BL/6 mice were fed a high-fat diet (HFD) to induce NAFLD and were treated with IIC (60, 120 or 240 mg/kg) for 8 weeks. Growth parameters, abdominal fat content, serum biochemical markers, hepatic lipid accumulation and insulin tolerance were assessed. Quantitative real-time PCR was used to determine the hepatic gene expression of TLR2, TLR4, TNF-α, IL-6, and IL-1ß. Western blot analysis was performed to determine the expression of the epidermal tight junction proteins ZO-1 and occludin. Gut microbiota profiles were established via high-throughput sequencing of the V3-V4 region of the bacterial 16S rRNA gene. RESULTS: IIC significantly reduced the severity of NAFLD induced by HFD in a dose-dependent manner. IIC decreased the ratio of Firmicutes/Bacteroidetes, reduced the relative abundance of Desulfovibrio and increased the relative abundance of Akkermansia. The intestinal barrier was improved as evidenced by the upregulation of the expression of ZO-1 and occludin in the ileum. IIC thus reduced the entry of LPS into the circulation and decreased the hepatic gene expression levels of proinflammatory cytokines. CONCLUSION: This approach demonstrated the positive effects of IIC in a mouse model of NAFLD, indicating that it possibly acts by reducing inflammation and improving the intestinal barrier function.


Asunto(s)
Microbioma Gastrointestinal/efectos de los fármacos , Compuestos Heterocíclicos de Anillo en Puente/farmacología , Enfermedad del Hígado Graso no Alcohólico/tratamiento farmacológico , Saponinas/farmacología , Triterpenos/farmacología , Animales , Citocinas/metabolismo , Dieta Alta en Grasa/efectos adversos , Relación Dosis-Respuesta a Droga , Quimioterapia Combinada , Microbioma Gastrointestinal/genética , Hepatitis Animal/tratamiento farmacológico , Compuestos Heterocíclicos de Anillo en Puente/administración & dosificación , Intestinos/efectos de los fármacos , Masculino , Ratones Endogámicos C57BL , Enfermedad del Hígado Graso no Alcohólico/etiología , Sustancias Protectoras/farmacología , ARN Ribosómico 16S , Saponinas/administración & dosificación , Triterpenos/administración & dosificación , Proteína de la Zonula Occludens-1/genética , Proteína de la Zonula Occludens-1/metabolismo
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